Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,910,487 C→A 12.1% A83A (GCG→GCT)  atpD ← F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,910,4870CA12.1% 854.5 / 62.1 244A83A (GCG→GCT) atpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (103/111);  new base A (16/14);  total (119/125)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

ACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAGCTGCTGCTGAACTTCCAGCACCAGACGCTCATTACCATT  >  CP009273/3910338‑3910636
                                                                                                                                                     |                                                                                                                                                     
aCTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTc                                                                                                                                                       <  2:2904048/150‑1 (MQ=255)
aCTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTc                                                                                                                                                       <  2:714378/150‑1 (MQ=255)
 cTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCg                                                                                                                                                      <  1:1198889/150‑1 (MQ=255)
 cTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCg                                                                                                                                                      >  1:3842399/1‑150 (MQ=255)
      aTACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTta                                                                                                                                                 >  2:3711926/1‑150 (MQ=255)
         ccGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtacct                                                                                                                                              >  2:2281570/1‑150 (MQ=255)
          cGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtaccta                                                                                                                                             <  1:3910800/150‑1 (MQ=255)
          cGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtaccta                                                                                                                                             >  2:665822/1‑150 (MQ=255)
           ggTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtacctac                                                                                                                                            >  1:326920/1‑150 (MQ=255)
           ggTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTtacctaa                                                                                                                                            >  2:48178/1‑149 (MQ=255)
              ttCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCgg                                                                                                                                         <  2:4046125/150‑1 (MQ=255)
                   gcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCAAGTACGTTCATGATACGGCCCAGAGTAGCTTTACCTACCGGGACtt                                                                                                                                    <  2:1082587/150‑1 (MQ=255)
                       ttCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAAt                                                                                                                                <  2:3097547/150‑1 (MQ=255)
                         ccTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCg                                                                                                                              >  2:4570314/1‑150 (MQ=255)
                          cTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCgg                                                                                                                             <  1:1574087/150‑1 (MQ=255)
                          cTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCgg                                                                                                                             <  2:3932470/150‑1 (MQ=255)
                           tGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCggg                                                                                                                            <  2:3579056/150‑1 (MQ=255)
                            gagaGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGgt                                                                                                                           <  2:4348885/150‑1 (MQ=255)
                              gagTTTGGCAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGgtgt                                                                                                                         >  2:144776/1‑150 (MQ=255)
                                gTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTc                                                                                                                       <  2:906394/150‑1 (MQ=255)
                                 tttGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCg                                                                                                                      <  1:4502158/150‑1 (MQ=255)
                                 tttGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTAGCTTTACCTACCGGGACTTCAATCGGGTGTTCg                                                                                                                      <  2:2599134/150‑1 (MQ=255)
                                   tGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAg                                                                                                                    <  1:2992429/150‑1 (MQ=255)
                                      cAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTc                                                                                                                 <  2:418171/150‑1 (MQ=255)
                                          tctTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTAACGACCGGGACTTCAAACGGGTGTTCGAGGTCtttt                                                                                                             >  1:3920405/1‑150 (MQ=255)
                                             tCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACa                                                                                                          <  1:690286/150‑1 (MQ=255)
                                              cGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACAt                                                                                                         <  2:2177937/150‑1 (MQ=255)
                                                 aGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCa                                                                                                      <  2:1521256/150‑1 (MQ=255)
                                                 aGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCa                                                                                                      <  1:2351501/150‑1 (MQ=255)
                                                 aGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAACCGGGACGTCAATCGGGTGTTCGAGGTCTTTTTCATCCa                                                                                                      >  2:465180/1‑150 (MQ=255)
                                                      ggTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTAACTACCGGGACATCAATCGGGTGTTCGAGGTGTTTTACATACAGACcg                                                                                                 >  2:3890377/1‑150 (MQ=255)
                                                       gTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTAGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACcgc                                                                                                <  1:1290119/150‑1 (MQ=255)
                                                          ctgcGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTAGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGAc                                                                                             >  2:1628756/1‑150 (MQ=255)
                                                          ctgcGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTAGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGAc                                                                                             <  2:2523851/150‑1 (MQ=255)
                                                             cgcgGTGAATCGCCCAACGCTCTTCTTCACCGTTCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCa                                                                                          <  1:65627/150‑1 (MQ=255)
                                                             cgcgGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCa                                                                                          <  2:20693/150‑1 (MQ=255)
                                                             cgcgGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCa                                                                                          <  2:768898/150‑1 (MQ=255)
                                                              gcgGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAg                                                                                         <  2:2723040/150‑1 (MQ=255)
                                                               cgGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCGACCGGGACATCAAACGGGTGTTAGAGGTCTTTTACATACAGACCGCGACCCAGa                                                                                        >  1:4103193/1‑150 (MQ=255)
                                                               cgGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAACCGGGACTTAAATCGGGTGTTGGAGGTGTTTTACATCCAGACCCCGACGCAGa                                                                                        >  1:827385/1‑150 (MQ=255)
                                                                ggTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTAGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGAc                                                                                       >  1:4114652/1‑150 (MQ=255)
                                                                ggTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTAGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGAc                                                                                       >  1:1406086/1‑150 (MQ=255)
                                                                 gTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGAcc                                                                                      <  2:3281972/150‑1 (MQ=255)
                                                                 gTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGAcc                                                                                      <  1:3783666/150‑1 (MQ=255)
                                                                   gAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGt                                                                                    <  2:2704815/150‑1 (MQ=255)
                                                                   gAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAACCCGGACTTCAATAGGGTGTTCGAGGTCTTTTACATACAGACCCCGACGCAGACCGt                                                                                    >  2:4028991/1‑150 (MQ=255)
                                                                   gAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTAACAACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTAAATACAGACCGCGACGCAGAccct                                                                                    >  1:929459/1‑148 (MQ=255)
                                                                    aaTCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACTGCCCAGAGTAGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTc                                                                                   <  2:1371505/150‑1 (MQ=255)
                                                                     aTCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCg                                                                                  <  1:1505631/150‑1 (MQ=255)
                                                                      tCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCgg                                                                                 <  2:4373061/150‑1 (MQ=255)
                                                                        gCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTGTTTTACATCCAGACCGCGACGCCGACCGTCggag                                                                               >  1:2582523/1‑150 (MQ=255)
                                                                               gCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCa                                                                        <  2:937904/150‑1 (MQ=255)
                                                                               gCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCa                                                                        <  1:3537414/150‑1 (MQ=255)
                                                                                   ttcttcACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTAACGACCCGGCCTTCAAACGGGTGTTCGAGGTCGTTTTCAACCAGACCGCGACCCAGACCGGCGGCGGAACCCCtttc                                                                    >  1:3477564/1‑148 (MQ=255)
                                                                                    tcttcACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTAAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCAATGCg                                                                   >  2:3497243/1‑150 (MQ=255)
                                                                                       tcACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAACCGGGACGTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCAATGCGATg                                                                >  1:4632246/1‑150 (MQ=255)
                                                                                        cACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCCTCGGAGGAACCCATTGCGATgg                                                               >  2:1768885/1‑150 (MQ=255)
                                                                                          ccGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGgta                                                             <  2:1947156/150‑1 (MQ=255)
                                                                                           cGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGgtac                                                            <  1:963991/150‑1 (MQ=255)
                                                                                            gATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTAACGACCGGGACATCAATCGGGGGTTAGCGGTCGTTTACATACAGACCGCGACGCAGACCGTCGGAGGGACCCATTGCGATGgttcg                                                           >  1:2045483/1‑150 (MQ=255)
                                                                                             aTCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCGACCGGGACGTCAATCGGGTGTTAGAGGGCGTTTAAATCCAGACCCCGACGCCGAACCGCGGAGGAAACCCTTGCGAAGgtacgt                                                          >  1:1861033/1‑150 (MQ=255)
                                                                                              tctcGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGgtacgta                                                         <  2:4001665/150‑1 (MQ=255)
                                                                                               ctcGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTAGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGgtacgtac                                                        >  1:3373577/1‑150 (MQ=255)
                                                                                                  gCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGAt                                                     <  2:414024/150‑1 (MQ=255)
                                                                                                  gCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGAt                                                     <  1:2209281/150‑1 (MQ=255)
                                                                                                   ccTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATa                                                    <  2:53836/150‑1 (MQ=255)
                                                                                                   ccTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAAACGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATa                                                    >  2:369752/1‑150 (MQ=255)
                                                                                                   ccTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCTACCGGGACGTCAATAGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGGACCCATTGCGATGGTACGTACGATa                                                    >  2:4578531/1‑150 (MQ=255)
                                                                                                    ctgtCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATAc                                                   <  2:4031992/147‑1 (MQ=255)
                                                                                                    cTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCGAACGGGCCTTCAAACCGGTGTTCGAGGTCTTTTACATCCATACCGCGACGCAGACCGTGGGAGGAACCCCATGCGATGGGACGTACGATAc                                                   >  1:1679096/1‑150 (MQ=255)
                                                                                                     tttCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTAAATCGGGTTTTCGAGGTCTTTTAAAACCAGAACGCGAAGCAGACCGTCGGAGGAACCCAATTCGATGGTACGTTCCATaac                                                  >  1:1912714/1‑148 (MQ=255)
                                                                                                      ttCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATAccg                                                 <  1:1034825/150‑1 (MQ=255)
                                                                                                        cATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTAGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATAccgcc                                               <  1:3501236/150‑1 (MQ=255)
                                                                                                         aTGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTAGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATAccgccg                                              >  1:2636806/1‑150 (MQ=255)
                                                                                                           gTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATAccgccgcc                                            <  2:488193/150‑1 (MQ=255)
                                                                                                             cGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGa                                          <  2:285055/150‑1 (MQ=255)
                                                                                                                ccGGTTCACCCAGTACGTTCATTATACGGCCCAGAGTCGCTTTACCAACCCGGACTTCAATAGGGTGTTCGAGGTCTTTTACAAACAGACCGCGAAGCAGAACGACGGAGGAAACCAATGCGATGGTACGTACCAAACACCCGCCGatca                                       >  2:4435114/1‑147 (MQ=255)
                                                                                                                ccGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAgct                                       <  2:407902/150‑1 (MQ=255)
                                                                                                                ccGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAgct                                       <  2:4225999/150‑1 (MQ=255)
                                                                                                                 cGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCAATGCGATGGTACGTACGATACCGCCGCCGAgcag                                      >  2:4193131/1‑148 (MQ=255)
                                                                                                                 cGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATACAGACCGCCACGCAGACCGTCGGAGGAAACCAATGCGATTGTACGTACGATACCGCCGCCGAgctg                                      >  2:1257501/1‑150 (MQ=255)
                                                                                                                  ggTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTACAACCGGGACGTAAAAAGGGGGTTATAGGTGTTTTTAAAAAAGACCCCCACGCCGACCCGACGAGGAACCCAATGCGATTGGACGTAAGATAACGCCCCCGCgcggc                                     >  1:1556745/1‑150 (MQ=255)
                                                                                                                  ggTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCAACCGGGACTTACAAAGGGTGTTCGAGGTGTTTTAAAACCACACCGCGACGCAGACCCTCGGAGGAAACCAAAGCGATGGTTCGGACGATAACGCCCCCGCgcggc                                     >  1:2399755/1‑150 (MQ=255)
                                                                                                                    ttCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAgctgctg                                   <  1:2666694/150‑1 (MQ=255)
                                                                                                                         ccATTACTTTCATGAGACGTCCCAGAGTCGCTTTACCGACCGTGACTTGTATCTTGTGTTCGAGGTCTTTTACATCCAGACCGCGTCGCAGACCGTCGGAGGAACCCTTTGCGTTGGTACGTACGTTTCAGACGCCGAgctgctgattaa                              <  2:2305099/150‑6 (MQ=255)
                                                                                                                               cGTTCATGATACGGCCCAGAGTCGCGTTTCCTACCCGGGCGTCAAACGGGTGTTAGAGGTCTTTTTCATCCAGACCCCCACGCCGACCGTCGGCGGAACCCAATGCGCTGGTACGTACGATAACCCCGCCCAGCTGCTGGTGAACTTCCa                        >  1:4353290/1‑150 (MQ=255)
                                                                                                                                gTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAGCTGCTGCTGAACTTCCAg                       <  1:442960/150‑1 (MQ=255)
                                                                                                                                gTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTAAAACGGGTGTTCGAGGTATTTTACAACCAGACCGCGACGCAGACCCTCGGAGGAACCCCTTGCGATGGTACGTACGATACCGCCGCCGAGCTGCTGCTGAACATCCAg                       >  2:3721360/1‑150 (MQ=255)
                                                                                                                                 ttCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTAAATCCAGACCGCGACGCAGACCGGCGGAGGAACCCATTGCGATGGTACGTACGATACCCCCGCCGAGCTGCTGCTGAACTTCCAGc                      >  1:2651879/1‑150 (MQ=255)
                                                                                                                                   cATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATAGGGTGTTCCAGGTATTTTACATACAGAACGCGAACCAGACCCTCGGAGGAACCCAATGCGATGGTAACGACCATACCGCCCCCGAGCAGCAGCAGAACATCCAGCAc                    >  2:2004169/1‑150 (MQ=255)
                                                                                                                                   cATGATACGGCCCAGAGTCGCTTTACCGACCGGGACTTAAATCGGGTGTTCGAGGTATTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAGCTGCTGCTGAACTTCCAGCAc                    >  2:1856729/1‑150 (MQ=255)
                                                                                                                                     tGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAGCTGCTGCTGAACTTCCAGCACCa                  <  2:245843/150‑1 (MQ=255)
                                                                                                                                                 gagTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAGCTGCTGCTGAACTTCCAGCACCAGACGCTCATTAc      >  2:1308800/1‑150 (MQ=255)
                                                                                                                                                 gagTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAGCTGCTGCTGAACTTCCAGCACCAGACGCTCATTAc      >  1:313312/1‑150 (MQ=255)
                                                                                                                                                  agTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAGCTGCTGCTGAACTTCCAGCACCAGACGCTCATTAcc     >  1:3094696/1‑150 (MQ=255)
                                                                                                                                                   gTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAGCTGCTGCTGAACTTCCAGCACCAGACGCTCATTACCa    <  1:135906/150‑1 (MQ=255)
                                                                                                                                                    tCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAGCTGCTGCTGAACTTCCAGCACCAGACGCTCATTACCAt   <  1:957591/150‑1 (MQ=255)
                                                                                                                                                    tCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAGCTGCTGCTGAACTTCCAGCACCAGACGCTCATTACCAt   <  1:2052305/150‑1 (MQ=255)
                                                                                                                                                    tCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAGCTGCTGCTGAACTTCCAGCACCAGACGCTCATTACCAt   <  1:2145991/150‑1 (MQ=255)
                                                                                                                                                     cGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAGCTGCTGCTGAACTTCCAGCACCAGACGCTCATTACCAtt  >  2:2604650/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCCGAGCTGCTGCTGAACTTCCAGCACCAGACGCTCATTACCATT  >  CP009273/3910338‑3910636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑