Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,911,056 C→A 10.9% P190P (CCG→CCT)  atpG ← F1 sector of membrane‑bound ATP synthase, gamma subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,911,0560CA10.9% 1077.2 / 65.7 299P190P (CCG→CCT) atpGF1 sector of membrane‑bound ATP synthase, gamma subunit
Reads supporting (aligned to +/- strand):  ref base C (138/127);  new base A (18/15);  total (156/143)
Fisher's exact test for biased strand distribution p-value = 8.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 304 total aligned reads displayed.

TGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCC  >  CP009273/3910907‑3911205
                                                                                                                                                     |                                                                                                                                                     
tGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAAc                                                                                                                                                       >  1:2797862/1‑150 (MQ=255)
 gCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACg                                                                                                                                                      <  1:1599106/150‑1 (MQ=255)
 gCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACg                                                                                                                                                      <  1:2244356/150‑1 (MQ=255)
   tCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGgc                                                                                                                                                    >  2:4431524/1‑150 (MQ=255)
        ggCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGcagcag                                                                                                                                               >  1:4164653/1‑150 (MQ=255)
        ggCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAAAGGcagcag                                                                                                                                               <  2:2662149/150‑1 (MQ=255)
            aGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAgctgg                                                                                                                                           >  1:1043398/1‑150 (MQ=255)
               ttttCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAgctggctg                                                                                                                                        <  1:3117173/150‑1 (MQ=255)
               ttttCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAgctggctg                                                                                                                                        >  2:2842317/1‑150 (MQ=255)
                 ttCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGAt                                                                                                                                      >  1:4342257/1‑150 (MQ=255)
                    aaCCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGt                                                                                                                                   >  2:4414588/1‑150 (MQ=255)
                      ccACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAAAGGCAGCAGCTGGCTGATGGTCg                                                                                                                                 <  2:4100580/150‑1 (MQ=255)
                       cACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCgg                                                                                                                                >  1:1011700/1‑150 (MQ=255)
                          gCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAAc                                                                                                                             <  2:274188/150‑1 (MQ=255)
                          gCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAAAGGCAGCAGCTGGCTGATGGTCGGAAc                                                                                                                             <  2:193506/150‑1 (MQ=255)
                           cccTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAAcc                                                                                                                            <  1:3856535/150‑1 (MQ=255)
                             cTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGTATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAAAGGCAGCAGCTGGCTGATGGTCGGAACCTg                                                                                                                          <  2:1605922/150‑1 (MQ=255)
                             cTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTg                                                                                                                          >  2:2922747/1‑150 (MQ=255)
                             cTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTg                                                                                                                          <  1:4059584/150‑1 (MQ=255)
                              tGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAAAGGCAGCAGCTGGCTGATGGTCGGAACCTga                                                                                                                         <  1:3235785/150‑1 (MQ=255)
                              tGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGAACGTGTTCGTACAGGTAATCCCAGGATATATGATTCAGATCATCATCATCTGATGCCGGTAACGGCAGAAGAAGGCTGATGGTCGGAACCTga                                                                                                                         >  1:3384175/1‑150 (MQ=255)
                                aTAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTgaga                                                                                                                       >  1:2385717/1‑150 (MQ=255)
                                 tAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGAc                                                                                                                      >  1:698150/1‑150 (MQ=255)
                                 tAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGAc                                                                                                                      >  1:1114345/1‑150 (MQ=255)
                                 tAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGAc                                                                                                                      <  2:466023/150‑1 (MQ=255)
                                  aaaCCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACa                                                                                                                     <  2:1313831/150‑1 (MQ=255)
                                   aaCCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAAAGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACAt                                                                                                                    >  1:873485/1‑150 (MQ=255)
                                        gagaTTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGgtgt                                                                                                               <  2:2247576/150‑1 (MQ=255)
                                        gagaTTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGgtgt                                                                                                               <  2:3148800/150‑1 (MQ=255)
                                           aTTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTtaa                                                                                                            <  1:65695/150‑1 (MQ=255)
                                           aTTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTtaa                                                                                                            >  2:1175018/1‑150 (MQ=255)
                                            ttCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTtaat                                                                                                           <  1:1678328/150‑1 (MQ=255)
                                             tCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAAAGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTtaata                                                                                                          >  1:4330048/1‑150 (MQ=255)
                                              cGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTtaataa                                                                                                         >  1:3894266/1‑150 (MQ=255)
                                               gACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATaaa                                                                                                        <  1:2425884/150‑1 (MQ=255)
                                               gACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATaaa                                                                                                        >  2:388217/1‑150 (MQ=255)
                                                 cATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAAtt                                                                                                      >  2:1347443/1‑150 (MQ=255)
                                                  aTAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAAAGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAAttt                                                                                                     >  2:842386/1‑150 (MQ=255)
                                                   tAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATttg                                                                                                    >  1:4495194/1‑150 (MQ=255)
                                                       gacgCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGc                                                                                                >  2:4006915/1‑150 (MQ=255)
                                                         cgCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTg                                                                                              <  2:3188073/150‑1 (MQ=255)
                                                           cagcagGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCACAGACATGGTGTTAATAAATTTGTTGCTGAc                                                                                            >  1:2973008/1‑150 (MQ=255)
                                                            agcagGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACa                                                                                           >  2:536284/1‑150 (MQ=255)
                                                            agcagGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAAAGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACa                                                                                           <  2:2517023/150‑1 (MQ=255)
                                                              cagGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAAt                                                                                         <  2:932139/150‑1 (MQ=255)
                                                                    aTCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAg                                                                                   <  2:1511133/150‑1 (MQ=255)
                                                                     tCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGc                                                                                  <  2:797720/150‑1 (MQ=255)
                                                                      ccAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCt                                                                                 >  1:4226153/1‑150 (MQ=255)
                                                                       cAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCtt                                                                                >  2:3447989/1‑150 (MQ=255)
                                                                        aGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTg                                                                               >  1:3630534/1‑150 (MQ=255)
                                                                          cAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTc                                                                             <  1:1829864/150‑1 (MQ=255)
                                                                           aaCGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTcc                                                                            >  2:321185/1‑150 (MQ=255)
                                                                            aCGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCa                                                                           <  1:3546817/150‑1 (MQ=255)
                                                                             cGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAg                                                                          >  1:231399/1‑150 (MQ=255)
                                                                                cTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACg                                                                       <  2:352673/150‑1 (MQ=255)
                                                                                    ggATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCt                                                                   <  1:3822922/150‑1 (MQ=255)
                                                                                    ggATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCt                                                                   >  1:4119563/1‑150 (MQ=255)
                                                                                      aTCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTtc                                                                 <  1:1344789/150‑1 (MQ=255)
                                                                                       tCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTtcg                                                                >  2:1781843/1‑150 (MQ=255)
                                                                                         gggTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTtcgtc                                                              >  1:3924285/1‑150 (MQ=255)
                                                                                         gggTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTtcgtc                                                              <  2:4265063/150‑1 (MQ=255)
                                                                                           gTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTcgtcgt                                                            <  1:468449/150‑1 (MQ=255)
                                                                                           gTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAAAGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTcgtcgt                                                            >  1:4256820/1‑150 (MQ=255)
                                                                                                tACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGcc                                                       >  1:777677/1‑150 (MQ=255)
                                                                                                 aCAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCt                                                      >  1:3467640/1‑150 (MQ=255)
                                                                                                 aCAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCt                                                      >  1:2861947/1‑150 (MQ=255)
                                                                                                 aCAGGTAATCCCAGGATGTATGTGTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGTCATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCt                                                      <  2:4139281/150‑1 (MQ=255)
                                                                                                  cAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTg                                                     <  2:2571072/150‑1 (MQ=255)
                                                                                                       aaTCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACa                                                >  2:68807/1‑150 (MQ=255)
                                                                                                       aaTCCACGGAATTATGTTTCAGATCATCATCATCAGAAGCAGGAAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGATAATAAAATTGAAGATTACAATGTAAAGCTTGTCCAGACGTCCATCGTCGTAGGCCTGCAACa                                                >  1:324256/1‑150 (MQ=255)
                                                                                                        aTCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACAt                                               >  2:3507500/1‑150 (MQ=255)
                                                                                                             aGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCAGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACtt                                          <  1:3771245/150‑1 (MQ=255)
                                                                                                              ggATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACttt                                         <  2:2393472/150‑1 (MQ=255)
                                                                                                                   tATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCg                                    <  1:1419211/150‑1 (MQ=255)
                                                                                                                   tATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCg                                    <  2:3969729/150‑1 (MQ=255)
                                                                                                                   tATGTTTCAGATCATCATCATCTGATGCCGGTAAAGTCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAATCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCg                                    >  1:2732015/1‑150 (MQ=255)
                                                                                                                     tGTTTCAGATCATCATCATCTGATGCCGGTAAAGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGa                                  >  1:1388097/1‑150 (MQ=255)
                                                                                                                        ttCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCg                               <  1:4407090/150‑1 (MQ=255)
                                                                                                                        ttCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCg                               <  1:911122/150‑1 (MQ=255)
                                                                                                                        ttCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCg                               >  2:1477742/1‑150 (MQ=255)
                                                                                                                        ttCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCg                               <  1:1050011/150‑1 (MQ=255)
                                                                                                                        ttCAGATCATCATCATCTGATGCCGGTAAAGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCg                               <  1:2493358/150‑1 (MQ=255)
                                                                                                                         tCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGa                              >  1:1708019/1‑150 (MQ=255)
                                                                                                                         tCAGATCATCATCATCTGATGCCGGTAAAGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGa                              >  2:3096223/1‑150 (MQ=255)
                                                                                                                           aGATCATCATCATCTGATGCCGGTAAAGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATc                            >  1:586502/1‑150 (MQ=255)
                                                                                                                            gATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCa                           <  2:3125354/150‑1 (MQ=255)
                                                                                                                             atcatcatcatcTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACACTGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAg                          <  1:216813/150‑1 (MQ=255)
                                                                                                                              tcatcatcatcTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGt                         >  1:2931430/1‑150 (MQ=255)
                                                                                                                              tcatcatcatcTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGt                         <  1:4100072/150‑1 (MQ=255)
                                                                                                                              tcatcatcatcTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGt                         <  1:1792416/150‑1 (MQ=255)
                                                                                                                                atcatcatcTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTc                       >  2:428164/1‑150 (MQ=255)
                                                                                                                                 tcatcatcTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCg                      <  2:126268/150‑1 (MQ=255)
                                                                                                                                  catcatcTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGGTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCgg                     <  2:2639610/150‑1 (MQ=255)
                                                                                                                                   atcatcTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGa                    >  1:554169/1‑150 (MQ=255)
                                                                                                                                          gATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGaa             >  1:2994865/1‑150 (MQ=255)
                                                                                                                                          gATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCAGAGACTTGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAAAATCACTTTTACCGTAACGATCAGTTCGGACAGGGaa             <  2:279605/150‑1 (MQ=255)
                                                                                                                                              ccGGTAAAGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGt         >  2:2096020/1‑150 (MQ=255)
                                                                                                                                                 gTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTAt      <  1:1923659/150‑1 (MQ=255)
                                                                                                                                                 gTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGGTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTAt      >  2:1113891/1‑150 (MQ=255)
                                                                                                                                                  tAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATc     <  2:3733055/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCATCTGATGCCGGTAACGGCAGCAGCTGGCTGATGGTCGGAACCTGAGACATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCC  >  CP009273/3910907‑3911205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑