Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,960,036 G→A 100% R87H (CGC→CAC)  rho → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,960,0360GA96.2% 728.2 / 12.8 212R87H (CGC→CAC) rhotranscription termination factor
Reads supporting (aligned to +/- strand):  ref base G (4/4);  new base A (97/107);  total (101/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 212 total aligned reads displayed.

CTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACT  >  CP009273/3959891‑3960182
                                                                                                                                                 |                                                                                                                                                  
cTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGt                                                                                                                                                >  1:4260132/1‑150 (MQ=255)
cTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGt                                                                                                                                                <  2:3802553/150‑1 (MQ=255)
cTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGt                                                                                                                                                >  1:99789/1‑150 (MQ=255)
 tGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGtt                                                                                                                                               <  2:4062454/150‑1 (MQ=255)
  gAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGttt                                                                                                                                              >  1:2884777/1‑150 (MQ=255)
    agcagcACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCa                                                                                                                                            >  2:780439/1‑150 (MQ=255)
    agcagcACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCa                                                                                                                                            >  1:2786595/1‑150 (MQ=255)
      cagcaCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAAc                                                                                                                                          <  2:613768/150‑1 (MQ=255)
        gcaCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCt                                                                                                                                        >  1:33807/1‑150 (MQ=255)
            gCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGc                                                                                                                                    >  2:666306/1‑150 (MQ=255)
             cAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCa                                                                                                                                   >  2:15948/1‑150 (MQ=255)
               aaGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACt                                                                                                                                 >  2:2943502/1‑150 (MQ=255)
               aaGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACt                                                                                                                                 >  2:2219613/1‑150 (MQ=255)
                agagTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTg                                                                                                                                >  2:3520374/1‑150 (MQ=255)
                  agTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGt                                                                                                                              <  2:3317079/150‑1 (MQ=255)
                    tGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGa                                                                                                                            >  1:2279663/1‑150 (MQ=255)
                     ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGAt                                                                                                                           <  2:4510170/150‑1 (MQ=255)
                       cGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATAc                                                                                                                         >  2:4267224/1‑150 (MQ=255)
                        gAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                        <  1:668310/150‑1 (MQ=255)
                        gAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                        >  2:1269057/1‑150 (MQ=255)
                         aaGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCa                                                                                                                       >  1:3765249/1‑150 (MQ=255)
                         aaGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCa                                                                                                                       <  1:1669853/150‑1 (MQ=255)
                           gATATCTTTGGTGATGGCGTACTGGAGATAGTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCAtc                                                                                                                     <  2:22289/150‑1 (MQ=255)
                            atatCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCAtct                                                                                                                    >  1:1001340/1‑150 (MQ=255)
                                cTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTgg                                                                                                                >  2:2814237/1‑150 (MQ=255)
                                cTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTgg                                                                                                                >  2:2472058/1‑150 (MQ=255)
                                cTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTgg                                                                                                                <  1:859963/150‑1 (MQ=255)
                                cTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTgg                                                                                                                <  1:2958607/150‑1 (MQ=255)
                                 tttGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGt                                                                                                               >  1:550979/1‑150 (MQ=255)
                                    ggTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAg                                                                                                            <  1:1903820/150‑1 (MQ=255)
                                     gTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGa                                                                                                           >  2:4110669/1‑150 (MQ=255)
                                     gTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGa                                                                                                           >  1:3992892/1‑150 (MQ=255)
                                      tGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAt                                                                                                          <  2:4546711/150‑1 (MQ=255)
                                       gATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAtt                                                                                                         >  1:842150/1‑150 (MQ=255)
                                            cGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgccc                                                                                                    <  1:3479363/150‑1 (MQ=255)
                                            cGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgccc                                                                                                    <  2:2026352/150‑1 (MQ=255)
                                            cGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgccc                                                                                                    <  2:2421632/150‑1 (MQ=255)
                                             gTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccg                                                                                                   <  1:3113471/150‑1 (MQ=255)
                                                    agaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAaag                                                                                            <  2:1586546/150‑1 (MQ=255)
                                                    agaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAaag                                                                                            <  2:2549890/150‑1 (MQ=255)
                                                     gaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAaaga                                                                                           <  2:2673709/150‑1 (MQ=255)
                                                     gaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAaaga                                                                                           <  2:3693452/150‑1 (MQ=255)
                                                      atatTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAaagaa                                                                                          <  2:3701533/150‑1 (MQ=255)
                                                      atatTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAaagaa                                                                                          >  2:117470/1‑150 (MQ=255)
                                                           gCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGa                                                                                     <  1:30777/150‑1 (MQ=255)
                                                           gCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGa                                                                                     <  1:30686/150‑1 (MQ=255)
                                                            cAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGaa                                                                                    >  1:1618169/1‑150 (MQ=255)
                                                                atggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCt                                                                                >  1:4303587/1‑150 (MQ=255)
                                                                 tggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTa                                                                               >  1:1436177/1‑150 (MQ=255)
                                                                 tggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTa                                                                               <  1:4426738/150‑1 (MQ=255)
                                                                 tggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTa                                                                               <  1:2099053/150‑1 (MQ=255)
                                                                   gatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAtt                                                                             <  2:893684/150‑1 (MQ=255)
                                                                    atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAttt                                                                            >  2:1453228/1‑150 (MQ=255)
                                                                    atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAttt                                                                            >  1:2591449/1‑150 (MQ=255)
                                                                    atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAttt                                                                            >  1:1792538/1‑150 (MQ=255)
                                                                        ggTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcg                                                                        <  1:484355/150‑1 (MQ=255)
                                                                        ggTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcg                                                                        >  1:3317341/1‑150 (MQ=255)
                                                                         gTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcgc                                                                       >  2:2158115/1‑150 (MQ=255)
                                                                         gTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcgc                                                                       >  2:3961650/1‑150 (MQ=255)
                                                                         gTTTCCTCCGTTCCGCAGACAGCTCATACCTCGCCGGTCCTGATGACATCTACGTATCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcgc                                                                       >  2:1888220/1‑150 (MQ=255)
                                                                          tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgct                                                                      >  1:3757188/1‑150 (MQ=255)
                                                                           ttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctg                                                                     >  2:3257877/1‑150 (MQ=255)
                                                                             cctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgct                                                                   <  1:984475/150‑1 (MQ=255)
                                                                                 cGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaaa                                                               >  1:1695237/1‑150 (MQ=255)
                                                                                 cGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaaa                                                               <  1:598502/150‑1 (MQ=255)
                                                                                  gTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAg                                                              >  2:1443405/1‑150 (MQ=255)
                                                                                  gTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAg                                                              >  1:4341508/1‑150 (MQ=255)
                                                                                   ttCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGt                                                             >  2:3657609/1‑150 (MQ=255)
                                                                                    tCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt                                                            <  2:2413769/150‑1 (MQ=255)
                                                                                          gACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGaa                                                      >  1:2391310/1‑150 (MQ=255)
                                                                                           aCAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAg                                                     >  2:2873826/1‑150 (MQ=255)
                                                                                           aCAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAg                                                     >  1:3182918/1‑150 (MQ=255)
                                                                                              gCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTa                                                  <  2:2094084/150‑1 (MQ=255)
                                                                                               cTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTaa                                                 <  1:981271/150‑1 (MQ=255)
                                                                                                 ccTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACt                                               >  1:4162170/1‑150 (MQ=255)
                                                                                                  cTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACtt                                              >  1:4542932/1‑150 (MQ=255)
                                                                                                  cTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACtt                                              >  1:530303/1‑150 (MQ=255)
                                                                                                   tACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTc                                             <  1:1521926/150‑1 (MQ=255)
                                                                                                    aCCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCg                                            >  2:3141486/1‑150 (MQ=255)
                                                                                                    aCCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCg                                            >  2:3175874/1‑150 (MQ=255)
                                                                                                       tCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACa                                         >  2:3962611/1‑150 (MQ=255)
                                                                                                        cGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACaa                                        <  1:1385643/150‑1 (MQ=255)
                                                                                                        cGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACaa                                        <  1:3725290/150‑1 (MQ=255)
                                                                                                            ggTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCt                                    >  1:864559/1‑150 (MQ=255)
                                                                                                                      aCATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGccc                          >  2:349881/1‑150 (MQ=255)
                                                                                                                          cTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaa                      <  2:217568/150‑1 (MQ=255)
                                                                                                                           tACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGTTAAGATTCGCCCGCCGAAAGAAGGTTAACGCTATTTTGCGCTGCTTAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaac                     <  1:2606557/150‑1 (MQ=255)
                                                                                                                              gTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaa                  <  2:1379955/150‑1 (MQ=255)
                                                                                                                                 tCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATc               >  2:4313621/1‑150 (MQ=255)
                                                                                                                                 tCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATc               >  2:2969189/1‑150 (MQ=255)
                                                                                                                                 tCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATc               >  2:3776209/1‑150 (MQ=255)
                                                                                                                                   cccTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCct             <  2:3476969/150‑1 (MQ=255)
                                                                                                                                        gCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTg        >  2:3283456/1‑150 (MQ=255)
                                                                                                                                          cAAATCCACCGTTTCAACCTCCGCACTGGTTATACCATCTCTGGTAAGATTCGCCCGCCTAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACTAATTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgag      <  1:3907455/150‑1 (MQ=255)
                                                                                                                                          cAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgag      <  1:4058649/150‑1 (MQ=255)
                                                                                                                                          cAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgag      <  2:4566202/150‑1 (MQ=255)
                                                                                                                                          cAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgag      <  2:657023/150‑1 (MQ=255)
                                                                                                                                          cAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgag      >  1:3504912/1‑150 (MQ=255)
                                                                                                                                          cAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgag      <  2:2070723/150‑1 (MQ=255)
                                                                                                                                              tCCACCGTTTCAACCTCCGCACTGTTTATACCATCTCTGGTAAGATTCGCCCGCCGAAAGATGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGATGTTAATTTCGAAAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACt  <  1:907391/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACT  >  CP009273/3959891‑3960182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑