Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,050,102 G→T 9.4% A431A (GCC→GCA)  glnA ← glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,050,1020GT 9.4% 1151.9 / 56.2 310A431A (GCC→GCA) glnAglutamine synthetase
Reads supporting (aligned to +/- strand):  ref base G (117/163);  new base T (12/18);  total (129/181)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

AGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCTTGATACC  >  CP009273/4049954‑4050251
                                                                                                                                                    |                                                                                                                                                     
aGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCgg                                                                                                                                                      >  2:4453229/1‑150 (MQ=255)
   cGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCtt                                                                                                                                                   <  2:2804633/150‑1 (MQ=255)
   cGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCtt                                                                                                                                                   <  2:1680600/150‑1 (MQ=255)
       aaGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAg                                                                                                                                               >  2:3642015/1‑150 (MQ=255)
         gTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGa                                                                                                                                             >  2:2633305/1‑150 (MQ=255)
         gTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGa                                                                                                                                             >  1:153956/1‑150 (MQ=255)
         gTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGa                                                                                                                                             >  2:1074517/1‑150 (MQ=255)
           ttCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAAc                                                                                                                                           <  2:143451/150‑1 (MQ=255)
            tCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACt                                                                                                                                          <  1:961448/150‑1 (MQ=255)
             ccACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTc                                                                                                                                         <  2:1725502/150‑1 (MQ=255)
                   cAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTc                                                                                                                                   <  1:2564493/150‑1 (MQ=255)
                    aaCTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTcc                                                                                                                                  >  2:1675923/1‑150 (MQ=255)
                      cTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAg                                                                                                                                >  2:763072/1‑150 (MQ=255)
                             acTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAgt                                                                                                                         <  2:4165905/150‑1 (MQ=255)
                               ttAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAgttc                                                                                                                       <  2:722165/150‑1 (MQ=255)
                                  gACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAgttcgtt                                                                                                                    <  2:284850/150‑1 (MQ=255)
                                    cGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCa                                                                                                                  <  2:3834591/150‑1 (MQ=255)
                                     gCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAg                                                                                                                 >  1:738691/1‑150 (MQ=255)
                                        gtagtaCAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGc                                                                                                              >  2:1177432/1‑150 (MQ=255)
                                        gtagtaCAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGc                                                                                                              >  2:1643969/1‑150 (MQ=255)
                                          agtaCAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGctt                                                                                                            >  1:530417/1‑150 (MQ=255)
                                           gtaCAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGcttc                                                                                                           <  1:4402127/150‑1 (MQ=255)
                                            taCAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGcttct                                                                                                          <  2:4397278/150‑1 (MQ=255)
                                             aCAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGcttctt                                                                                                         <  1:1921817/150‑1 (MQ=255)
                                             aCAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGcttctt                                                                                                         >  2:2557814/1‑150 (MQ=255)
                                              cAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCttcttc                                                                                                        <  2:1606555/150‑1 (MQ=255)
                                                gCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCa                                                                                                      <  1:1601959/150‑1 (MQ=255)
                                                gCTCAAACTCGACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCa                                                                                                      <  2:1797739/150‑1 (MQ=255)
                                                gCTCAAACTATACCGGATGCGGAGTCATACGCACGCGGTCAACTTCTTCGAGACGCAGAGCGATGTACTCATCAATTGCTTCGTCAGTTAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGATCAGTGATACTTCCa                                                                                                      >  1:1290301/1‑150 (MQ=255)
                                                  tCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCaga                                                                                                    >  2:3557766/1‑150 (MQ=255)
                                                       ctctACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGcc                                                                                               <  2:559452/150‑1 (MQ=255)
                                                        tctACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCt                                                                                              <  1:2282772/150‑1 (MQ=255)
                                                        tctACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCt                                                                                              <  2:2689023/150‑1 (MQ=255)
                                                             cGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAAc                                                                                         >  1:3017922/1‑150 (MQ=255)
                                                                aTGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCtg                                                                                      <  1:2619673/150‑1 (MQ=255)
                                                                aTGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCtg                                                                                      <  1:4301051/150‑1 (MQ=255)
                                                                 tGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCtgt                                                                                     <  2:2023625/150‑1 (MQ=255)
                                                                     gAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGa                                                                                 <  2:2644692/150‑1 (MQ=255)
                                                                        tCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGAtct                                                                              <  2:3600559/150‑1 (MQ=255)
                                                                        tCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGAtct                                                                              <  1:1592737/150‑1 (MQ=255)
                                                                         cATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGAtctc                                                                             <  1:406355/150‑1 (MQ=255)
                                                                         cATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGAtctc                                                                             >  2:3664905/1‑150 (MQ=255)
                                                                          aTACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATctct                                                                            <  2:4089261/150‑1 (MQ=255)
                                                                              gcacgcGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCg                                                                        <  1:4628318/150‑1 (MQ=255)
                                                                               cacgcGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGc                                                                       >  1:4452962/1‑150 (MQ=255)
                                                                                   cgGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGcttct                                                                   <  1:342161/150‑1 (MQ=255)
                                                                                    ggTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGcttctt                                                                  >  2:1332195/1‑150 (MQ=255)
                                                                                     gTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCttcttc                                                                 <  1:1032327/150‑1 (MQ=255)
                                                                                      tCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTtcttct                                                                >  1:1298316/1‑150 (MQ=255)
                                                                                      tCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTtcttct                                                                <  2:626952/150‑1 (MQ=255)
                                                                                       cATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTg                                                               >  1:2684603/1‑150 (MQ=255)
                                                                                          cttcttCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTggcg                                                            <  1:3148343/150‑1 (MQ=255)
                                                                                                cgcgACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGt                                                      <  1:1675923/150‑1 (MQ=255)
                                                                                                 gcgACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTc                                                     <  1:36724/150‑1 (MQ=255)
                                                                                                   gACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCAt                                                   >  2:4239518/1‑150 (MQ=255)
                                                                                                   gACGCAGAGAGATGTACGCATCAATTGCTTCGTCAGTGAACAAGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCAt                                                   <  1:1167569/150‑1 (MQ=255)
                                                                                                    aCGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATa                                                  >  2:1747939/1‑150 (MQ=255)
                                                                                                    aCGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATa                                                  <  2:4298046/150‑1 (MQ=255)
                                                                                                     cGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACTGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATAc                                                 <  1:3061908/150‑1 (MQ=255)
                                                                                                     cGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATAc                                                 >  1:1827364/1‑150 (MQ=255)
                                                                                                       cAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAg                                               >  2:1396106/1‑150 (MQ=255)
                                                                                                       cAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAg                                               >  2:3427533/1‑150 (MQ=255)
                                                                                                           gCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGttt                                           <  2:2750677/150‑1 (MQ=255)
                                                                                                             gATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGttttt                                         <  2:4566486/150‑1 (MQ=255)
                                                                                                             gATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGttttt                                         <  1:2178441/150‑1 (MQ=255)
                                                                                                              aTGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTg                                        <  1:4064545/150‑1 (MQ=255)
                                                                                                              aTGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCCTCTTCTGGCGGCAGGTCATACAGGTTTTTg                                        <  1:2378328/150‑1 (MQ=255)
                                                                                                               tGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGt                                       >  1:4177620/1‑150 (MQ=255)
                                                                                                               tGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGt                                       >  2:649403/1‑150 (MQ=255)
                                                                                                                gTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTc                                      >  1:898113/1‑150 (MQ=255)
                                                                                                                gTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTc                                      >  2:251852/1‑150 (MQ=255)
                                                                                                                gTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTc                                      <  1:2889579/150‑1 (MQ=255)
                                                                                                                 tACGCATCAATTGCTTAGTCAGTGAACACGCCTCCGGCTTTCAGGTTCTCGCTGTGCAGTTCCAGTTAGTTCTGTGCTTCTTCCTGTGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCTGCAGGTATTACAGGTTTTTGTcc                                     <  2:2289980/150‑1 (MQ=255)
                                                                                                                  aCGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCa                                    <  2:1148829/150‑1 (MQ=255)
                                                                                                                  aCGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCa                                    >  2:1139083/1‑150 (MQ=255)
                                                                                                                  aCGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCa                                    <  2:1902200/150‑1 (MQ=255)
                                                                                                                   cGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCAt                                   <  2:2300526/150‑1 (MQ=255)
                                                                                                                    gCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATg                                  <  2:3363220/150‑1 (MQ=255)
                                                                                                                     cATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATgg                                 <  1:2833411/150‑1 (MQ=255)
                                                                                                                      aTCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGc                                <  1:3471236/150‑1 (MQ=255)
                                                                                                                       tCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCt                               >  2:3942225/1‑150 (MQ=255)
                                                                                                                        cAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCtt                              >  1:2415381/1‑150 (MQ=255)
                                                                                                                        cAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCtt                              >  2:1215063/1‑150 (MQ=255)
                                                                                                                         aaTTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTc                             >  2:3258801/1‑150 (MQ=255)
                                                                                                                          aTTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGACCTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCg                            >  1:4575544/1‑150 (MQ=255)
                                                                                                                          aTTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCATTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCg                            <  1:1381642/150‑1 (MQ=255)
                                                                                                                              cTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCg                        <  1:3710074/150‑1 (MQ=255)
                                                                                                                                  gTCAGTGAACACGCCACCGGCTTTCAGGAANTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCggatg                    >  1:4280408/1‑150 (MQ=255)
                                                                                                                                        gAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCtt              <  1:3335474/150‑1 (MQ=255)
                                                                                                                                        gAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTGTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTAGTCCATGGCTTCGCCCGGATGGATCtt              >  2:2196942/1‑150 (MQ=255)
                                                                                                                                          acacGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGt            >  1:1175662/1‑150 (MQ=255)
                                                                                                                                          acacGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGt            <  2:874009/150‑1 (MQ=255)
                                                                                                                                            acGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTc          <  1:490407/150‑1 (MQ=255)
                                                                                                                                              gCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCtt        <  1:91587/150‑1 (MQ=255)
                                                                                                                                               ccACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCTTg       >  2:3491889/1‑150 (MQ=255)
                                                                                                                                                cACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCTTGa      >  2:2373818/1‑150 (MQ=255)
                                                                                                                                                 aCCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCTTGAt     >  2:3011460/1‑150 (MQ=255)
                                                                                                                                                  ccGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCTTGATa    <  1:2722590/150‑1 (MQ=255)
                                                                                                                                                  ccGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCTTGATa    <  1:3597063/150‑1 (MQ=255)
                                                                                                                                                    ggCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCTTGATAcc  <  1:3856429/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCTTGATACC  >  CP009273/4049954‑4050251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑