Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,054,222 T→A 18.2% V139D (GTT→GAT)  yihL → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,054,2220TA18.2% 689.6 / 143.1 270V139D (GTT→GAT) yihLputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (103/118);  new base A (20/29);  total (123/147)
Fisher's exact test for biased strand distribution p-value = 5.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 287 total aligned reads displayed.

AATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGG  >  CP009273/4054073‑4054371
                                                                                                                                                     |                                                                                                                                                     
aaTTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGt                                                                                                                                                       >  2:2905362/1‑150 (MQ=255)
 aTTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGtt                                                                                                                                                      >  1:4384327/1‑150 (MQ=255)
 aTTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGtt                                                                                                                                                      >  1:3045624/1‑150 (MQ=255)
  ttGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGatt                                                                                                                                                     <  1:3365985/150‑1 (MQ=255)
   tGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGattt                                                                                                                                                    >  1:1030786/1‑150 (MQ=255)
   tGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGattt                                                                                                                                                    >  1:3026244/1‑150 (MQ=255)
    gAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTc                                                                                                                                                   >  2:1604169/1‑150 (MQ=255)
    gAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTc                                                                                                                                                   >  2:1293123/1‑150 (MQ=255)
     aGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCt                                                                                                                                                  <  1:1019398/150‑1 (MQ=255)
     aGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCt                                                                                                                                                  <  1:4339159/150‑1 (MQ=255)
      gCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGATTTCTa                                                                                                                                                 <  2:4172900/150‑1 (MQ=255)
       cAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTAt                                                                                                                                                <  1:2950289/150‑1 (MQ=255)
        aaGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATc                                                                                                                                               >  2:921628/1‑150 (MQ=255)
        aaGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGATTTCTATc                                                                                                                                               <  1:1178313/150‑1 (MQ=255)
          gggCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCAc                                                                                                                                             >  1:4212037/1‑150 (MQ=255)
          gggCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCAc                                                                                                                                             >  1:3808790/1‑150 (MQ=255)
              gagaGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGaaa                                                                                                                                         >  1:371580/1‑150 (MQ=255)
               agagCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAAc                                                                                                                                        >  1:3162084/1‑150 (MQ=255)
               agagCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAAc                                                                                                                                        <  2:2654973/150‑1 (MQ=255)
                 agCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACat                                                                                                                                      <  1:1634238/150‑1 (MQ=255)
                   ccTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACatat                                                                                                                                    <  1:1295765/150‑1 (MQ=255)
                      tCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATAtt                                                                                                                                 <  1:1567916/150‑1 (MQ=255)
                         tGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAAt                                                                                                                              <  2:765464/150‑1 (MQ=255)
                         tGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAAt                                                                                                                              >  2:1820111/1‑150 (MQ=255)
                         tGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAAt                                                                                                                              <  2:434461/150‑1 (MQ=255)
                             ggTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTg                                                                                                                          >  2:3213937/1‑150 (MQ=255)
                              gTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGa                                                                                                                         <  2:2409819/150‑1 (MQ=255)
                               tttACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGaa                                                                                                                        <  2:4222810/150‑1 (MQ=255)
                                 tACCTAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGAGCCACTGATTGCATTGACGCCATCACCTGATCTCTATCGCATAACCGGCTGGGGGGAGCTGGAAGGACATAAAGATTTCTATCACGAAACATATATTAATCCTGAAGt                                                                                                                      <  2:2068713/150‑1 (MQ=255)
                                   ccGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTg                                                                                                                    >  1:3265072/1‑150 (MQ=255)
                                   ccGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTg                                                                                                                    >  1:4033995/1‑150 (MQ=255)
                                     gagaAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAGGTTGCt                                                                                                                  <  1:3375040/150‑1 (MQ=255)
                                     gagaAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCt                                                                                                                  <  2:1177687/150‑1 (MQ=255)
                                     gagaAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGATTTCTATCACGAAACATATATTAATCCTGAAGTTGCt                                                                                                                  <  2:352042/150‑1 (MQ=255)
                                     gagaAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGATTTCTATCACGAAACATATATTAATCCTGAAGTTGCt                                                                                                                  >  1:453168/1‑150 (MQ=255)
                                       gaAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTcc                                                                                                                <  1:1497870/150‑1 (MQ=255)
                                       gaAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTcc                                                                                                                <  1:799597/150‑1 (MQ=255)
                                            aCCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCt                                                                                                           <  2:4530384/150‑1 (MQ=255)
                                              cGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCttt                                                                                                         >  1:2887008/1‑150 (MQ=255)
                                               gTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTa                                                                                                        <  1:2152380/150‑1 (MQ=255)
                                                 aCCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGATTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTAtt                                                                                                      >  2:4466484/1‑150 (MQ=255)
                                                        atatTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAACGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGaacaac                                                                                               <  2:3116776/150‑1 (MQ=255)
                                                        atatTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGaacaac                                                                                               >  2:1366049/1‑150 (MQ=255)
                                                         tatTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACt                                                                                              <  2:3072413/150‑1 (MQ=255)
                                                          atTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACtt                                                                                             <  1:1247628/150‑1 (MQ=255)
                                                          atTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACtt                                                                                             <  1:1406538/150‑1 (MQ=255)
                                                          atTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACtt                                                                                             >  2:1220986/1‑150 (MQ=255)
                                                             cccGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGATTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGaa                                                                                          <  1:930345/150‑1 (MQ=255)
                                                                 agaCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAAcc                                                                                      >  1:3958550/1‑150 (MQ=255)
                                                                  gaCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCa                                                                                     <  2:2524530/150‑1 (MQ=255)
                                                                  gaCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCa                                                                                     >  2:1085017/1‑150 (MQ=255)
                                                                    cGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACt                                                                                   <  1:1264236/150‑1 (MQ=255)
                                                                            ccACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCt                                                                           <  1:2301174/150‑1 (MQ=255)
                                                                               cTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCa                                                                        >  2:594275/1‑150 (MQ=255)
                                                                                tGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAg                                                                       >  1:3113280/1‑150 (MQ=255)
                                                                                    tGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTGTCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTg                                                                   >  2:2350680/1‑150 (MQ=255)
                                                                                      cAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTggg                                                                 >  2:3638375/1‑150 (MQ=255)
                                                                                       aGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGa                                                                >  1:2491689/1‑150 (MQ=255)
                                                                                        gTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAGAACCACTCATTTTCTGCAGTCTGGGaa                                                               <  2:3740779/150‑1 (MQ=255)
                                                                                        gTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGaa                                                               <  1:3441230/150‑1 (MQ=255)
                                                                                         tGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGaaa                                                              <  2:2830382/150‑1 (MQ=255)
                                                                                            cGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGt                                                           >  2:1441649/1‑150 (MQ=255)
                                                                                             gCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTg                                                          >  2:4404376/1‑150 (MQ=255)
                                                                                             gCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTg                                                          >  1:535577/1‑150 (MQ=255)
                                                                                               cATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGATTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCt                                                        <  1:1694892/150‑1 (MQ=255)
                                                                                                        aaCTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGATTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAag                                               <  2:724985/150‑1 (MQ=255)
                                                                                                          ctctATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAagag                                             <  1:1006001/150‑1 (MQ=255)
                                                                                                             tATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGACAAGTGCTACCAAAAAGAGACg                                          >  1:4411405/1‑150 (MQ=255)
                                                                                                             tATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACg                                          >  1:3873340/1‑150 (MQ=255)
                                                                                                              aTCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACgg                                         <  2:3958550/150‑1 (MQ=255)
                                                                                                                  cATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGgtagt                                     >  2:2759378/1‑150 (MQ=255)
                                                                                                                  cATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGgtagt                                     <  1:4569691/150‑1 (MQ=255)
                                                                                                                    tCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTaa                                   <  2:1585987/150‑1 (MQ=255)
                                                                                                                     cACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTaaa                                  >  2:4456192/1‑150 (MQ=255)
                                                                                                                      aCCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTaaaa                                 >  2:4000525/1‑150 (MQ=255)
                                                                                                                       ccGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTaaaaa                                <  1:9980/150‑1 (MQ=255)
                                                                                                                           cTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAAtt                            <  2:4140804/150‑1 (MQ=255)
                                                                                                                           cTGGGGGGCGCTGGAAGGACATAAAGATTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAAtt                            >  2:18288/1‑150 (MQ=255)
                                                                                                                            tGGGGGGCGCTGGAAGGACATAAAGATTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTg                           <  1:638172/150‑1 (MQ=255)
                                                                                                                             ggggggCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGa                          >  2:3430826/1‑150 (MQ=255)
                                                                                                                              gggggCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGAt                         <  2:3783156/150‑1 (MQ=255)
                                                                                                                              gggggCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGT‑AAAAAATTGAtt                        <  1:679919/150‑1 (MQ=255)
                                                                                                                                   cgcTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCa                    <  2:1331576/150‑1 (MQ=255)
                                                                                                                                    gcTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCaa                   >  1:1036709/1‑150 (MQ=255)
                                                                                                                                     cTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCaaa                  <  1:1880331/150‑1 (MQ=255)
                                                                                                                                      tGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAAc                 >  1:3328051/1‑150 (MQ=255)
                                                                                                                                       ggAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAAcc                >  2:1643315/1‑150 (MQ=255)
                                                                                                                                        gAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAAccc               <  1:2372622/150‑1 (MQ=255)
                                                                                                                                         aaGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCg              >  1:4095076/1‑150 (MQ=255)
                                                                                                                                          aGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGt             >  1:558172/1‑150 (MQ=255)
                                                                                                                                          aGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGt             <  1:32837/150‑1 (MQ=255)
                                                                                                                                           ggACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTc            >  1:2643701/1‑150 (MQ=255)
                                                                                                                                            gACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCa           <  2:3376822/150‑1 (MQ=255)
                                                                                                                                            gACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCa           <  1:2217350/150‑1 (MQ=255)
                                                                                                                                             aCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAg          >  1:2324595/1‑150 (MQ=255)
                                                                                                                                              cATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGa         >  2:678484/1‑150 (MQ=255)
                                                                                                                                               aTAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGaa        <  2:3563068/150‑1 (MQ=255)
                                                                                                                                                 aaaGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATg      <  1:3213937/150‑1 (MQ=255)
                                                                                                                                                     ttttCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCgg  <  1:3556045/150‑1 (MQ=255)
                                                                                                                                                     ttttCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCgg  <  1:3556089/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGG  >  CP009273/4054073‑4054371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑