Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,058,809 G→T 10.1% I106I (ATC→ATA)  yihO ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,058,8090GT10.1% 1018.8 / 55.2 276I106I (ATC→ATA) yihOputative transporter
Reads supporting (aligned to +/- strand):  ref base G (134/114);  new base T (13/15);  total (147/129)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

GCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAG  >  CP009273/4058660‑4058958
                                                                                                                                                     |                                                                                                                                                     
 ccGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGa                                                                                                                                                      >  1:3908281/1‑150 (MQ=255)
    cgAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATaa                                                                                                                                                   <  1:3099062/150‑1 (MQ=255)
      aGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAAc                                                                                                                                                 <  2:1373525/150‑1 (MQ=255)
            gcgcgcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGc                                                                                                                                           <  1:234178/150‑1 (MQ=255)
            gcgcgcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGc                                                                                                                                           <  1:1855794/150‑1 (MQ=255)
             cgcgcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCg                                                                                                                                          >  1:593830/1‑150 (MQ=255)
              gcgcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGt                                                                                                                                         >  2:2353290/1‑150 (MQ=255)
               cgcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGtt                                                                                                                                        <  1:1435094/150‑1 (MQ=255)
                   cATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCaa                                                                                                                                    >  2:3138944/1‑150 (MQ=255)
                   cATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACT‑GAGCGTTGCAAt                                                                                                                                   >  2:3138798/1‑150 (MQ=255)
                    aTTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAAt                                                                                                                                   >  2:3515182/1‑150 (MQ=255)
                    aTTCGGG‑TTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAAtt                                                                                                                                  >  1:1685379/1‑150 (MQ=255)
                     ttCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAAtt                                                                                                                                  <  1:1389203/150‑1 (MQ=255)
                     ttCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAAtt                                                                                                                                  <  2:518758/150‑1 (MQ=255)
                     ttCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAAtt                                                                                                                                  <  2:2191715/150‑1 (MQ=255)
                     ttCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAAtt                                                                                                                                  <  1:2963472/150‑1 (MQ=255)
                      tCGTGTTTTTTGTATTTGCCGGGATCATCGCACCATACGAGCATTTCATCAGACTGTATGAAAGTCCAAACATCTTAAACAGAGAGGTGGCAATCGTCGTTTTAACCTTCAAACAAAAGGTGTTGGCGATAAACTTTTGCGTTGGAATTa                                                                                                                                 <  2:200555/150‑1 (MQ=255)
                          gTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAAcgc                                                                                                                             >  2:567324/1‑150 (MQ=255)
                           tttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAAcgcc                                                                                                                            <  2:4490981/150‑1 (MQ=255)
                           tttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAAcgcc                                                                                                                            <  1:1308711/150‑1 (MQ=255)
                            ttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAAcgccg                                                                                                                           <  2:3328328/150‑1 (MQ=255)
                              ttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACgccgcc                                                                                                                         <  2:3792414/150‑1 (MQ=255)
                               tGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGccgccg                                                                                                                        >  2:320791/1‑150 (MQ=255)
                                 gTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGa                                                                                                                      >  1:2815480/1‑150 (MQ=255)
                                  tAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGaa                                                                                                                     >  1:3687026/1‑150 (MQ=255)
                                  tAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGaa                                                                                                                     >  1:2881557/1‑150 (MQ=255)
                                    aTTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAAcc                                                                                                                   <  1:1553329/150‑1 (MQ=255)
                                     ttGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCg                                                                                                                  <  1:87701/150‑1 (MQ=255)
                                      tGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGc                                                                                                                 >  1:457383/1‑150 (MQ=255)
                                      tGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGc                                                                                                                 >  1:4111106/1‑150 (MQ=255)
                                           ggATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCat                                                                                                            <  1:4460156/150‑1 (MQ=255)
                                            gATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGTCGGAACCGCTGCata                                                                                                           >  2:4221275/1‑150 (MQ=255)
                                              tcatcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCAtata                                                                                                         >  2:4011939/1‑150 (MQ=255)
                                              tcatcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCAtata                                                                                                         >  1:2593771/1‑150 (MQ=255)
                                                atcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaa                                                                                                       >  2:3059121/1‑150 (MQ=255)
                                                atcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATaaaaa                                                                                                       >  2:2137616/1‑150 (MQ=255)
                                                 tcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaa                                                                                                      <  2:1540968/150‑1 (MQ=255)
                                                   gCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaatt                                                                                                    >  1:4341965/1‑149 (MQ=255)
                                                   gCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaata                                                                                                    >  1:2014536/1‑150 (MQ=255)
                                                    cACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaataa                                                                                                   <  1:3593377/150‑1 (MQ=255)
                                                       cATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAgg                                                                                                <  2:3189435/150‑1 (MQ=255)
                                                        aTACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAggg                                                                                               >  1:2248574/1‑150 (MQ=255)
                                                            gAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGa                                                                                           <  2:4241255/150‑1 (MQ=255)
                                                              gCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGaaa                                                                                         <  2:950861/150‑1 (MQ=255)
                                                               cAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTTGAGCGTTGCAATTAAAGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAt                                                                                        >  1:1024746/1‑150 (MQ=255)
                                                                aGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAtt                                                                                       >  1:849080/1‑150 (MQ=255)
                                                                 gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAttt                                                                                      <  1:4580471/150‑1 (MQ=255)
                                                                    catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGcc                                                                                   >  1:1154562/1‑150 (MQ=255)
                                                                    catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGcc                                                                                   >  1:1506486/1‑150 (MQ=255)
                                                                     atcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCt                                                                                  >  2:1001287/1‑150 (MQ=255)
                                                                      tcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCtt                                                                                 <  2:1439031/150‑1 (MQ=255)
                                                                          aCTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAgg                                                                             <  2:1817092/150‑1 (MQ=255)
                                                                            tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGcc                                                                           <  1:1711495/150‑1 (MQ=255)
                                                                            tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGcc                                                                           <  1:3376861/150‑1 (MQ=255)
                                                                            tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGcc                                                                           >  2:4559529/1‑150 (MQ=255)
                                                                             gTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGccc                                                                          <  2:3163885/150‑1 (MQ=255)
                                                                             gTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGccc                                                                          >  2:703263/1‑150 (MQ=255)
                                                                             gTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTATAGGccc                                                                          >  2:2770239/1‑150 (MQ=255)
                                                                               aTGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCaa                                                                        >  2:2824884/1‑150 (MQ=255)
                                                                                 gAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAACATAAAGGGACGAAATTTGCCTTTAGGCCCAata                                                                      <  2:499912/150‑1 (MQ=255)
                                                                                  aaaGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAatat                                                                     <  2:911069/150‑1 (MQ=255)
                                                                                   aaGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAtt                                                                    >  1:2636181/1‑150 (MQ=255)
                                                                                       ccAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTc                                                                >  2:1248942/1‑150 (MQ=255)
                                                                                        cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTcc                                                               >  2:3337080/1‑150 (MQ=255)
                                                                                                 tAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcga                                                      <  2:2998042/150‑1 (MQ=255)
                                                                                                 tAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcga                                                      <  2:4028658/150‑1 (MQ=255)
                                                                                                  aaaCAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcgag                                                     >  2:1417973/1‑150 (MQ=255)
                                                                                                  aaaCAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcgag                                                     >  1:757240/1‑150 (MQ=255)
                                                                                                   aaCAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcgagt                                                    >  2:1365375/1‑150 (MQ=255)
                                                                                                      aGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTaaa                                                 <  1:654710/150‑1 (MQ=255)
                                                                                                        cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTaaaaa                                               <  1:4148760/150‑1 (MQ=255)
                                                                                                         gcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAAt                                              <  1:3760241/150‑1 (MQ=255)
                                                                                                          cgGTGGAAATAGTCGTTTTTACCTGCAAACAAAAGGTGGTGTCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAATGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATc                                             <  1:2104662/150‑1 (MQ=255)
                                                                                                           ggTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATcc                                            <  1:3485582/150‑1 (MQ=255)
                                                                                                           ggTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATcc                                            >  1:761192/1‑150 (MQ=255)
                                                                                                               gCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTg                                        >  2:2630802/1‑150 (MQ=255)
                                                                                                               gCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTg                                        >  2:1660852/1‑150 (MQ=255)
                                                                                                                 aaTCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAg                                      >  1:4374959/1‑150 (MQ=255)
                                                                                                                 aaTCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAg                                      <  2:3433417/150‑1 (MQ=255)
                                                                                                                 aaTCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAg                                      <  1:4152383/150‑1 (MQ=255)
                                                                                                                    cgtcgtTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCat                                   <  1:2988034/150‑1 (MQ=255)
                                                                                                                     gtcgtTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCata                                  <  1:3942888/150‑1 (MQ=255)
                                                                                                                        gtTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCg                               >  1:3196019/1‑150 (MQ=255)
                                                                                                                        gtTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCg                               >  2:871248/1‑150 (MQ=255)
                                                                                                                          tttAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGt                             <  1:2482008/150‑1 (MQ=255)
                                                                                                                             aaCCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTaaa                          >  2:4139042/1‑150 (MQ=255)
                                                                                                                                 ggCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAcgcg                      <  2:3649602/150‑1 (MQ=255)
                                                                                                                                 ggCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAcgcg                      >  2:808181/1‑150 (MQ=255)
                                                                                                                                  gCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCgg                     <  2:869443/150‑1 (MQ=255)
                                                                                                                                   caaacaaaAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGt                    >  1:1097562/1‑150 (MQ=255)
                                                                                                                                   caaacaaaAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGt                    >  2:2248156/1‑150 (MQ=255)
                                                                                                                                    aaacaaaAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTa                   >  1:3534310/1‑150 (MQ=255)
                                                                                                                                     aacaaaAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaa                  <  1:1930285/150‑1 (MQ=255)
                                                                                                                                     aacaaaAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaa                  <  1:4538083/150‑1 (MQ=255)
                                                                                                                                     aacaaaAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaa                  <  1:4257734/150‑1 (MQ=255)
                                                                                                                                     aacaaaAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaa                  <  1:2382259/150‑1 (MQ=255)
                                                                                                                                              tggtggCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCg         <  1:3996268/150‑1 (MQ=255)
                                                                                                                                              tggtggCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCg         >  1:3728684/1‑150 (MQ=255)
                                                                                                                                                gtggCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGcc       <  2:1675397/150‑1 (MQ=255)
                                                                                                                                                     gATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAg  <  2:569816/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAG  >  CP009273/4058660‑4058958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑