BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,210,835	G→C	14.3%	C527W (TGC→TGG)	arpA ←	ankyrin repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,210,835	0	G	C	14.3%	765.0 / 117.8	224	C527W (TGC→TGG)	arpA	ankyrin repeat protein
Reads supporting (aligned to +/- strand): ref base G (107/85); new base C (15/17); total (122/102)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 232 total aligned reads displayed.

CGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCTGGA  >  CP009273/4210688‑4210984

    tCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCa                                                                                                                                                 <  1:3752989/150‑1 (MQ=255)

     cgcgcgCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCAt                                                                                                                                                >  2:3388175/1‑150 (MQ=255)

            aaGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATc                                                                                                                                         <  2:3535269/150‑1 (MQ=255)

                   ttAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGcc                                                                                                                                  <  2:1357713/150‑1 (MQ=255)

                   ttAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGcc                                                                                                                                  <  2:4455526/150‑1 (MQ=255)

                   ttAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGcc                                                                                                                                  <  1:1667729/150‑1 (MQ=255)

                    tAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCt                                                                                                                                 >  2:217482/1‑150 (MQ=255)

                    tAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCt                                                                                                                                 >  2:218432/1‑150 (MQ=255)

                    tAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCt                                                                                                                                 <  1:3453744/150‑1 (MQ=255)

                    tAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCt                                                                                                                                 <  1:3081113/150‑1 (MQ=255)

                      gCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTc                                                                                                                               <  1:996170/150‑1 (MQ=255)

                        ggTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAg                                                                                                                             <  1:3418128/150‑1 (MQ=255)

                          tCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGga                                                                                                                           >  1:1303768/1‑150 (MQ=255)

                          tCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGga                                                                                                                           <  1:1556171/150‑1 (MQ=255)

                           cAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGgag                                                                                                                          >  2:4157815/1‑150 (MQ=255)

                            agaagaTCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGgaga                                                                                                                         <  2:3688018/150‑1 (MQ=255)

                             gaagaTCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTAGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGAt                                                                                                                        >  2:2509461/1‑150 (MQ=255)

                               agaTCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCg                                                                                                                      >  1:4213225/1‑150 (MQ=255)

                                gaTCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGa                                                                                                                     >  1:4265365/1‑150 (MQ=255)

                                       aaTATCGGCAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAAAGCTTCAAGAATAACGTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTCCCTg                                                                                                              >  2:287594/1‑150 (MQ=255)

                                       aaTATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTg                                                                                                              >  2:287662/1‑150 (MQ=255)

                                        atatCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGt                                                                                                             >  2:626929/1‑150 (MQ=255)

                                         tatCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGtt                                                                                                            <  1:2211083/150‑1 (MQ=255)

                                            cGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCtt                                                                                                         <  1:1418282/150‑1 (MQ=255)

                                            cGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCtt                                                                                                         >  1:4246320/1‑150 (MQ=255)

                                              gAATCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTa                                                                                                       <  2:2407430/150‑1 (MQ=255)

                                              gAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTa                                                                                                       <  1:2431438/150‑1 (MQ=255)

                                                aGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGt                                                                                                     <  1:1196884/150‑1 (MQ=255)

                                                            aaTTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACAtgt                                                                                         >  2:1401335/1‑150 (MQ=255)

                                                               ttCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCg                                                                                      <  2:3152226/150‑1 (MQ=255)

                                                                 ccTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGaa                                                                                    >  2:29966/1‑150 (MQ=255)

                                                                  cTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAAt                                                                                   >  1:3357900/1‑150 (MQ=255)

                                                                  cTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAAt                                                                                   <  1:4160101/150‑1 (MQ=255)

                                                                  cTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAAt                                                                                   <  1:2397570/150‑1 (MQ=255)

                                                                   tGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAAtt                                                                                  <  2:512583/150‑1 (MQ=255)

                                                                    gggCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTa                                                                                 >  2:2868423/1‑150 (MQ=255)

                                                                       cTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACa                                                                              >  1:883182/1‑150 (MQ=255)

                                                                         aGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACaaa                                                                            >  2:2566896/1‑150 (MQ=255)

                                                                         aGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACaaa                                                                            >  2:2894940/1‑150 (MQ=255)

                                                                            cTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGt                                                                         >  2:2732929/1‑150 (MQ=255)

                                                                             tGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTa                                                                        >  2:2664031/1‑150 (MQ=255)

                                                                               ggCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAAc                                                                      <  2:1013496/150‑1 (MQ=255)

                                                                               ggCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAAc                                                                      <  1:148698/150‑1 (MQ=255)

                                                                                gCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAAcg                                                                     >  1:2020970/1‑150 (MQ=255)

                                                                                 cAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAAcgc                                                                    >  2:3387976/1‑150 (MQ=255)

                                                                                      cTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCa                                                               >  2:4496929/1‑150 (MQ=255)

                                                                                       ttCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCaa                                                              >  1:1006572/1‑150 (MQ=255)

                                                                                        tCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCaaa                                                             <  1:1953980/150‑1 (MQ=255)

                                                                                          aaGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAAc                                                           <  2:1940029/150‑1 (MQ=255)

                                                                                          aaGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAAc                                                           >  2:122987/1‑150 (MQ=255)

                                                                                           aGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACa                                                          <  1:3562989/150‑1 (MQ=255)

                                                                                           aGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACa                                                          <  1:251288/150‑1 (MQ=255)

                                                                                             aaTAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAAt                                                        >  1:3255877/1‑150 (MQ=255)

                                                                                               tAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAAttt                                                      >  1:236663/1‑150 (MQ=255)

                                                                                                 aCTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTc                                                    >  2:1186811/1‑150 (MQ=255)

                                                                                                  cTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCa                                                   <  1:3122397/150‑1 (MQ=255)

                                                                                                   tttCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAg                                                  <  2:3206989/150‑1 (MQ=255)

                                                                                                      cACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCAt                                               >  1:1195198/1‑150 (MQ=255)

                                                                                                         gATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTc                                            >  1:1115994/1‑150 (MQ=255)

                                                                                                          atatCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCg                                           <  2:2335880/150‑1 (MQ=255)

                                                                                                          atatCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCg                                           <  1:1296217/150‑1 (MQ=255)

                                                                                                          atatCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCg                                           >  2:1517407/1‑150 (MQ=255)

                                                                                                           tatCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCgg                                          >  2:3864863/1‑150 (MQ=255)

                                                                                                            atCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGc                                         >  2:4300182/1‑150 (MQ=255)

                                                                                                            atCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGc                                         >  1:2099285/1‑150 (MQ=255)

                                                                                                            atCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGc                                         >  1:3450347/1‑150 (MQ=255)

                                                                                                               gCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATc                                      <  1:1474153/150‑1 (MQ=255)

                                                                                                                  aTGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCAcc                                   >  1:2882881/1‑150 (MQ=255)

                                                                                                                  aTGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCAcc                                   >  1:3506056/1‑150 (MQ=255)

                                                                                                                     tCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCAtt                                >  2:2682906/1‑150 (MQ=255)

                                                                                                                      ccATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTc                               >  1:1030477/1‑150 (MQ=255)

                                                                                                                       cATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCg                              >  1:1450688/1‑150 (MQ=255)

                                                                                                                       cATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCg                              <  1:4334631/150‑1 (MQ=255)

                                                                                                                        aTTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGc                             >  1:1812682/1‑150 (MQ=255)

                                                                                                                        aTTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGc                             >  1:4051200/1‑150 (MQ=255)

                                                                                                                          tttGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCa                           <  2:2393377/150‑1 (MQ=255)

                                                                                                                             gCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGGTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATaa                        >  2:228240/1‑150 (MQ=255)

                                                                                                                              cATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAAc                       >  1:4008983/1‑150 (MQ=255)

                                                                                                                                tCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACAt                     >  2:402293/1‑150 (MQ=255)

                                                                                                                                 cGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATg                    >  1:735075/1‑150 (MQ=255)

                                                                                                                                 cGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATg                    <  1:4523374/150‑1 (MQ=255)

                                                                                                                                 cGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATg                    >  1:2615187/1‑150 (MQ=255)

                                                                                                                                  gCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACCTgg                   >  2:1071179/1‑150 (MQ=255)

                                                                                                                                   ccAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGt                  <  1:4097256/150‑1 (MQ=255)

                                                                                                                                     aGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGtat                >  1:4096765/1‑150 (MQ=255)

                                                                                                                                     aGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGtat                >  1:642987/1‑150 (MQ=255)

                                                                                                                                       atatAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATaa              <  1:3267702/150‑1 (MQ=255)

                                                                                                                                        tataGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATaaa             >  2:2057050/1‑150 (MQ=255)

                                                                                                                                        tataGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATaaa             >  2:1919750/1‑150 (MQ=255)

                                                                                                                                           aGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACa          <  1:4459899/150‑1 (MQ=255)

                                                                                                                                           aGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACa          >  1:3986719/1‑150 (MQ=255)

                                                                                                                                            gTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACAt         <  1:2134971/150‑1 (MQ=255)

                                                                                                                                            gTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACAGGGTATAAAACAt         >  2:187611/1‑150 (MQ=255)

                                                                                                                                             tCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATg        <  1:2702533/150‑1 (MQ=255)

                                                                                                                                              ccTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATgg       >  1:4602286/1‑150 (MQ=255)

                                                                                                                                                tGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAATACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCt     >  1:2816644/1‑150 (MQ=255)

                                                                                                                                                tGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCt     >  1:440885/1‑150 (MQ=255)

                                                                                                                                                 gggCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCTg    <  1:217482/150‑1 (MQ=255)

                                                                                                                                                 gggCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCTg    >  1:1676814/1‑150 (MQ=255)

                                                                                                                                                  ggCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCTgg   <  1:1091913/150‑1 (MQ=255)

CGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCTGGA  >  CP009273/4210688‑4210984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`