Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,211,576 G→T 100% S280R (AGC→AGA)  arpA ← ankyrin repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,211,5760GT98.5% 948.1 / 4.0 274S280R (AGC→AGA) arpAankyrin repeat protein
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (146/124);  total (148/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 279 total aligned reads displayed.

TTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGAT  >  CP009273/4211429‑4211722
                                                                                                                                                   |                                                                                                                                                  
ttttAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTctct                                                                                                                                                  <  2:912319/150‑1 (MQ=255)
ttttAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTctct                                                                                                                                                  <  2:2093789/150‑1 (MQ=255)
 tttAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTtctctc                                                                                                                                                 <  1:413495/150‑1 (MQ=255)
   tAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCAt                                                                                                                                               >  2:3343751/1‑150 (MQ=255)
   tAACATCAATAGCTGTTCTTTCGGAAGTGATGGTATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCAt                                                                                                                                               <  1:4150996/150‑1 (MQ=255)
      cATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAAt                                                                                                                                            >  1:3808465/1‑150 (MQ=255)
        acAATAGCTGTTCTTTCGGAAGTGATGGAATTATTGCAACAGAATGATTAATAACATGATCATTTGACCAATTCAGGAGGCTATAATATACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTc                                                                                                                                          >  2:3797024/2‑150 (MQ=255)
         cAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCt                                                                                                                                         >  2:1361528/1‑150 (MQ=255)
            tAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTAt                                                                                                                                      <  2:1689654/150‑1 (MQ=255)
               cTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTAtttt                                                                                                                                   >  2:448996/1‑150 (MQ=255)
                tGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTa                                                                                                                                  >  1:2539690/1‑150 (MQ=255)
                  ttCTTTCGGAATTGCTTGAATTATTGCAACTTATTTATTAATAACATGATCATTTGACGATTTCAGGAGGCTATATTCTACATTCATAAAATGCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCTTAATTCTTATTTtctc                                                                                                                                <  2:3169900/150‑1 (MQ=255)
                    cTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgt                                                                                                                              >  1:647721/1‑150 (MQ=255)
                    cTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgt                                                                                                                              >  1:2815737/1‑150 (MQ=255)
                     tttCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgtt                                                                                                                             <  1:1402332/150‑1 (MQ=255)
                      ttCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgtta                                                                                                                            <  1:3319182/150‑1 (MQ=255)
                      ttCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgtta                                                                                                                            >  1:3577000/1‑150 (MQ=255)
                         ggAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgttagtt                                                                                                                         <  1:4173328/150‑1 (MQ=255)
                           aaGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTAc                                                                                                                       >  2:3956559/1‑150 (MQ=255)
                             gTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACAt                                                                                                                     >  1:4055717/1‑150 (MQ=255)
                             gTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACAt                                                                                                                     <  1:4489957/150‑1 (MQ=255)
                               gCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTa                                                                                                                   <  2:3064921/150‑1 (MQ=255)
                                 tGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAgg                                                                                                                 >  2:32525/1‑150 (MQ=255)
                                    aaTTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATc                                                                                                              >  2:893286/1‑150 (MQ=255)
                                     attattGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCa                                                                                                             <  2:2137288/150‑1 (MQ=255)
                                     attattGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCa                                                                                                             <  2:4268983/150‑1 (MQ=255)
                                     attattGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCa                                                                                                             >  1:3452935/1‑150 (MQ=255)
                                      ttattGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCAt                                                                                                            >  1:3686165/1‑150 (MQ=255)
                                        attGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATaa                                                                                                          <  1:325555/150‑1 (MQ=255)
                                        attGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATaa                                                                                                          >  1:4049485/1‑150 (MQ=255)
                                        attGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATaa                                                                                                          <  2:3638682/150‑1 (MQ=255)
                                              aCAGATTGATTAATAACATGATCATTTGACNATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGaa                                                                                                    >  1:4283278/1‑150 (MQ=255)
                                              aCAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGaa                                                                                                    <  1:3947705/150‑1 (MQ=255)
                                              aCAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGaa                                                                                                    <  1:1623807/150‑1 (MQ=255)
                                               cAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGaaa                                                                                                   <  1:4416796/150‑1 (MQ=255)
                                                aGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAAc                                                                                                  >  1:3191818/1‑150 (MQ=255)
                                                      attAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTa                                                                                            <  1:1575104/150‑1 (MQ=255)
                                                      attAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTa                                                                                            >  1:1941751/1‑150 (MQ=255)
                                                       ttAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTaa                                                                                           <  1:734596/150‑1 (MQ=255)
                                                       ttAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTaa                                                                                           >  1:4057529/1‑150 (MQ=255)
                                                        taataaCATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAAt                                                                                          >  2:4129550/1‑150 (MQ=255)
                                                         aataaCATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATg                                                                                         >  2:2988146/1‑150 (MQ=255)
                                                           taaCATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATggg                                                                                       >  2:1510727/1‑150 (MQ=255)
                                                           taaCATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATggg                                                                                       <  1:1148472/150‑1 (MQ=255)
                                                           taaCATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATggg                                                                                       >  2:709548/1‑150 (MQ=255)
                                                               aTGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACa                                                                                   >  2:3299622/1‑150 (MQ=255)
                                                                  aTCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATcc                                                                                <  2:3111306/150‑1 (MQ=255)
                                                                    cATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCtt                                                                              <  2:623877/150‑1 (MQ=255)
                                                                    cATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCtt                                                                              <  2:2481003/150‑1 (MQ=255)
                                                                     aTTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTc                                                                             <  2:2299882/150‑1 (MQ=255)
                                                                      tttGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCa                                                                            <  2:2824411/150‑1 (MQ=255)
                                                                           ccATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTtgt                                                                       <  2:2168081/150‑1 (MQ=255)
                                                                            cATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTtgtg                                                                      <  2:555292/150‑1 (MQ=255)
                                                                            cATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTtgtg                                                                      <  2:3223448/150‑1 (MQ=255)
                                                                             aTTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTgtgt                                                                     >  1:2069415/1‑150 (MQ=255)
                                                                             aTTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTgtgt                                                                     >  1:1275810/1‑150 (MQ=255)
                                                                                 cAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCtt                                                                 >  1:2302172/1‑150 (MQ=255)
                                                                                 cAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCtt                                                                 >  2:497708/1‑150 (MQ=255)
                                                                                 cAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCtt                                                                 <  2:474786/150‑1 (MQ=255)
                                                                                  aggaggCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCttt                                                                >  2:2420826/1‑150 (MQ=255)
                                                                                  aggaggCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCttt                                                                <  1:1994266/150‑1 (MQ=255)
                                                                                  aggaggCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCttt                                                                <  1:3103185/150‑1 (MQ=255)
                                                                                  aggaggCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCttt                                                                <  2:2004157/150‑1 (MQ=255)
                                                                                     aggCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTAtt                                                             <  2:4519191/150‑1 (MQ=255)
                                                                                           taATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGc                                                       >  1:1035576/1‑150 (MQ=255)
                                                                                               cTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGa                                                   <  1:1211784/150‑1 (MQ=255)
                                                                                                tACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGAc                                                  <  2:1580759/150‑1 (MQ=255)
                                                                                                tACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGAc                                                  >  1:4564903/1‑150 (MQ=255)
                                                                                                 aCATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTACTGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGAcc                                                 >  2:3614648/1‑150 (MQ=255)
                                                                                                 aCATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGAcc                                                 >  2:2607968/1‑150 (MQ=255)
                                                                                                 aCATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGAcc                                                 >  2:2679092/1‑150 (MQ=255)
                                                                                                 aCATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGAcc                                                 >  1:264907/1‑150 (MQ=255)
                                                                                                   aTTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGt                                               <  2:1254981/150‑1 (MQ=255)
                                                                                                         aaaaTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGc                                         >  1:3448677/1‑150 (MQ=255)
                                                                                                          aaaTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCa                                        >  1:135738/1‑150 (MQ=255)
                                                                                                          aaaTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCa                                        >  2:1779710/1‑150 (MQ=255)
                                                                                                           aaTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCAt                                       >  2:3388176/1‑150 (MQ=255)
                                                                                                             tCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATgg                                     >  1:3794348/1‑150 (MQ=255)
                                                                                                                 tCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTAt                                 >  1:3756513/1‑150 (MQ=255)
                                                                                                                   agagAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATcc                               <  2:1033690/150‑1 (MQ=255)
                                                                                                                   agagAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATcc                               >  1:163868/1‑150 (MQ=255)
                                                                                                                    gagaATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATcca                              >  2:3710847/1‑150 (MQ=255)
                                                                                                                        aTAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAg                          >  2:2777837/1‑150 (MQ=255)
                                                                                                                           gtgtTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTaa                       <  1:484989/150‑1 (MQ=255)
                                                                                                                             gtTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAata                     <  1:4344389/150‑1 (MQ=255)
                                                                                                                               ttAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCTTTAGTTACATTAGGATCATAAACCGAAACCACGTATTGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATg                   <  1:3763148/150‑1 (MQ=255)
                                                                                                                               ttAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATg                   >  1:2803253/1‑150 (MQ=255)
                                                                                                                                tAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATgg                  <  1:1334543/150‑1 (MQ=255)
                                                                                                                                  atatTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCt                >  1:1743599/1‑150 (MQ=255)
                                                                                                                                  atatTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCt                <  2:4352860/150‑1 (MQ=255)
                                                                                                                                  atatTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCt                >  1:4068581/1‑150 (MQ=255)
                                                                                                                                   tatTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTg               >  1:1001144/1‑150 (MQ=255)
                                                                                                                                      tctcTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTCCATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCa            >  2:101911/1‑150 (MQ=255)
                                                                                                                                      tctcTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCa            <  1:3229026/150‑1 (MQ=255)
                                                                                                                                        tctTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATa          >  1:4302903/1‑150 (MQ=255)
                                                                                                                                          tttGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAac        <  1:1821889/150‑1 (MQ=255)
                                                                                                                                           ttGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAaca       <  1:3409261/150‑1 (MQ=255)
                                                                                                                                               tttCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGa   >  1:2811125/1‑150 (MQ=255)
                                                                                                                                               tttCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGa   <  1:549755/150‑1 (MQ=255)
                                                                                                                                               tttCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGa   >  2:139426/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGAT  >  CP009273/4211429‑4211722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑