Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,211,803 G→A 100% P205S (CCT→TCT)  arpA ← ankyrin repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,211,8030GA97.8% 974.9 / 6.7 278P205S (CCT→TCT) arpAankyrin repeat protein
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base A (143/129);  total (147/131)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 278 total aligned reads displayed.

CTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTC  >  CP009273/4211657‑4211949
                                                                                                                                                  |                                                                                                                                                  
    aTTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTg                                                                                                                                             <  1:1510727/150‑1 (MQ=255)
     ttCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTgg                                                                                                                                            <  2:2539690/150‑1 (MQ=255)
       cTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGaa                                                                                                                                          >  1:503126/1‑150 (MQ=255)
          aGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAAcc                                                                                                                                       <  2:729193/150‑1 (MQ=255)
            ccGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCaa                                                                                                                                     >  2:3018374/1‑150 (MQ=255)
            ccGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCaa                                                                                                                                     >  2:1355666/1‑150 (MQ=255)
             cGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAAc                                                                                                                                    <  2:2302172/150‑1 (MQ=255)
              ggACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACTGCTGGAAACCAACg                                                                                                                                   >  1:1758604/1‑150 (MQ=255)
              ggACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACg                                                                                                                                   >  1:3636636/1‑150 (MQ=255)
                aCCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGcc                                                                                                                                 <  2:4338007/150‑1 (MQ=255)
                    tCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAg                                                                                                                             >  1:4264732/1‑150 (MQ=255)
                      aaaGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTc                                                                                                                           >  1:3094202/1‑150 (MQ=255)
                       aaGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCt                                                                                                                          >  1:3043563/1‑150 (MQ=255)
                       aaGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCt                                                                                                                          <  2:3808465/150‑1 (MQ=255)
                         gCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCACAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTgg                                                                                                                        <  2:2246599/150‑1 (MQ=255)
                                 aTCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCa                                                                                                                >  1:913809/1‑150 (MQ=255)
                                   ccaccaGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCAtt                                                                                                              <  2:1035576/150‑1 (MQ=255)
                                   ccaccaGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCAtt                                                                                                              <  2:136170/150‑1 (MQ=255)
                                    caccaGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCAttt                                                                                                             <  2:1540262/150‑1 (MQ=255)
                                      ccaGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCAttttt                                                                                                           <  1:709548/150‑1 (MQ=255)
                                      ccaGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCAttttt                                                                                                           <  2:3758449/150‑1 (MQ=255)
                                       caGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCAtttttt                                                                                                          >  1:662962/1‑150 (MQ=255)
                                        aGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTttg                                                                                                         <  1:2420826/150‑1 (MQ=255)
                                         gTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTATGGTTTTTGCATTTTttgt                                                                                                        >  1:4589330/1‑150 (MQ=255)
                                             tatGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGaa                                                                                                    >  1:3910233/1‑150 (MQ=255)
                                              ctGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAg                                                                                                   <  2:1653770/149‑1 (MQ=255)
                                                  cTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCg                                                                                               <  1:842341/150‑1 (MQ=255)
                                                   tGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGt                                                                                              >  1:2911638/1‑150 (MQ=255)
                                                    gCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTg                                                                                             >  2:2994715/1‑150 (MQ=255)
                                                     ccATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTga                                                                                            <  2:4326316/150‑1 (MQ=255)
                                                           cacTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAAtt                                                                                      >  1:2111736/1‑150 (MQ=255)
                                                           cacTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAAtt                                                                                      <  1:1960290/150‑1 (MQ=255)
                                                             cTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAAtttt                                                                                    >  2:2062475/1‑150 (MQ=255)
                                                             cTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAAtttt                                                                                    <  1:1051907/150‑1 (MQ=255)
                                                             cTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAAtttt                                                                                    >  1:1709256/1‑150 (MQ=255)
                                                                aTGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCa                                                                                 <  2:4057529/150‑1 (MQ=255)
                                                                 tGCCATAAGATTCCATCTTGTCAAAATATGCTTTCAGAACTAGCCCGAAATCACCGCAGGCAGCACCGCAACGCCCATCAGAACGGCTGGAAACCAACGCCCAAGTCTGGGTTTTGCAGTTTTTGGTGAAGTGCGTGAGAAATTTTTCAg                                                                                >  2:1231925/1‑150 (MQ=255)
                                                                  gCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGc                                                                               >  2:4380468/1‑150 (MQ=255)
                                                                    cATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTg                                                                             >  1:2691438/1‑150 (MQ=255)
                                                                    cATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCGGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTg                                                                             >  1:924051/1‑150 (MQ=255)
                                                                     aTTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGa                                                                            >  2:3322951/1‑150 (MQ=255)
                                                                          aTTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGt                                                                       <  2:587380/150‑1 (MQ=255)
                                                                           ttCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTa                                                                      <  1:325828/150‑1 (MQ=255)
                                                                            tCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTaa                                                                     <  2:656403/150‑1 (MQ=255)
                                                                              cATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGTAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAatc                                                                   >  1:3249237/1‑150 (MQ=255)
                                                                              cATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAatc                                                                   >  2:3923642/1‑150 (MQ=255)
                                                                                tCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAatcat                                                                 <  2:3686165/150‑1 (MQ=255)
                                                                                    gTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTg                                                             <  2:3756845/150‑1 (MQ=255)
                                                                                        aaaaATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATgg                                                         <  2:1743599/150‑1 (MQ=255)
                                                                                        aaaTATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGGGTAATCATCTTGATgg                                                         >  2:3024706/1‑150 (MQ=255)
                                                                                        aaaTATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATgg                                                         >  2:3367060/1‑150 (MQ=255)
                                                                                        aaaTATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTATGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATgg                                                         >  2:1126502/1‑150 (MQ=255)
                                                                                         aaTATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATggg                                                        <  1:4447401/150‑1 (MQ=255)
                                                                                         aaTATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATggg                                                        >  1:2497018/1‑150 (MQ=255)
                                                                                          atatGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATgggg                                                       >  2:2103601/1‑150 (MQ=255)
                                                                                          atatGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATgggg                                                       >  2:878767/1‑150 (MQ=255)
                                                                                           tatGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGt                                                      <  1:2861225/150‑1 (MQ=255)
                                                                                           tatGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGt                                                      >  2:3195682/1‑150 (MQ=255)
                                                                                                 ttCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTaa                                                <  1:4072231/150‑1 (MQ=255)
                                                                                                 ttCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTaa                                                <  1:2268733/150‑1 (MQ=255)
                                                                                                 ttCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTaa                                                >  2:2788566/1‑150 (MQ=255)
                                                                                                 ttCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTaa                                                >  2:1830336/1‑150 (MQ=255)
                                                                                                  tCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAAt                                               <  2:1001144/150‑1 (MQ=255)
                                                                                                  tCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAAt                                               <  1:419010/150‑1 (MQ=255)
                                                                                                    aTAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCa                                             <  2:687978/150‑1 (MQ=255)
                                                                                                     tAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCaa                                            >  1:3486943/1‑150 (MQ=255)
                                                                                                       aCTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACa                                          <  2:2803266/150‑1 (MQ=255)
                                                                                                        cTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACAt                                         >  1:1885357/1‑150 (MQ=255)
                                                                                                        cTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACAt                                         >  1:13707/1‑150 (MQ=255)
                                                                                                         tAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGGTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACAtt                                        >  2:1284398/1‑150 (MQ=255)
                                                                                                          aGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTc                                       >  2:4463668/1‑150 (MQ=255)
                                                                                                           gCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCt                                      <  2:3970170/150‑1 (MQ=255)
                                                                                                           gCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCt                                      <  1:3387993/150‑1 (MQ=255)
                                                                                                            cccGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTg                                     <  2:2569005/150‑1 (MQ=255)
                                                                                                            cccGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTg                                     <  1:452977/150‑1 (MQ=255)
                                                                                                            cccGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTg                                     <  1:3718176/150‑1 (MQ=255)
                                                                                                              cGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGAt                                   <  2:357327/150‑1 (MQ=255)
                                                                                                               gAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATa                                  >  2:412947/1‑150 (MQ=255)
                                                                                                               gAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATa                                  >  2:2227942/1‑150 (MQ=255)
                                                                                                               gAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATa                                  <  2:1628939/150‑1 (MQ=255)
                                                                                                               gAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATa                                  <  1:1361528/150‑1 (MQ=255)
                                                                                                                   tCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAata                              >  1:4057498/1‑150 (MQ=255)
                                                                                                                   tCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAata                              >  1:2707185/1‑150 (MQ=255)
                                                                                                                    cACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAatat                             >  2:3049241/1‑150 (MQ=255)
                                                                                                                     aCCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATc                            >  2:4591832/1‑150 (MQ=255)
                                                                                                                       cGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCAt                          >  1:2169420/1‑150 (MQ=255)
                                                                                                                          aggcagCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTaa                       >  2:2337527/1‑150 (MQ=255)
                                                                                                                          aggcagCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTaa                       >  2:1772805/1‑150 (MQ=255)
                                                                                                                           ggcagCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAat                      <  1:3831004/150‑1 (MQ=255)
                                                                                                                                     caacgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTATGGTTTTTGCATATTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCAACTTGATGGGGTTTGTAATCAACATTCTGATAAAAATCATTAATATATAGTgca            >  1:1237775/1‑150 (MQ=255)
                                                                                                                                      aacgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTgcag           >  1:2149931/1‑150 (MQ=255)
                                                                                                                                      aacgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTgcag           <  2:337505/150‑1 (MQ=255)
                                                                                                                                       acgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTgcagc          >  1:2772086/1‑150 (MQ=255)
                                                                                                                                         gcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCaa        <  2:4177340/150‑1 (MQ=255)
                                                                                                                                            ccTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAAtgt     >  1:4141688/1‑150 (MQ=255)
                                                                                                                                             cTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAAtgtg    >  2:2454324/1‑150 (MQ=255)
                                                                                                                                             cTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAAGTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAAtgtg    >  2:2454221/1‑150 (MQ=255)
                                                                                                                                              tCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATgtgt   <  2:2811199/150‑1 (MQ=255)
                                                                                                                                               cAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTc  <  2:4049498/150‑1 (MQ=255)
                                                                                                                                               cAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTc  <  2:2864487/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTC  >  CP009273/4211657‑4211949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑