Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,274,748 C→A 18.0% R28L (CGC→CTC)  actP ← acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,274,7480CA18.0% 874.9 / 125.6 289R28L (CGC→CTC) actPacetate transporter
Reads supporting (aligned to +/- strand):  ref base C (137/100);  new base A (34/18);  total (171/118)
Fisher's exact test for biased strand distribution p-value = 3.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 307 total aligned reads displayed.

CCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGT  >  CP009273/4274599‑4274897
                                                                                                                                                     |                                                                                                                                                     
    cccGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGctc                                                                                                                                                   >  1:3701128/1‑150 (MQ=255)
       gTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTAc                                                                                                                                                >  1:2908387/1‑150 (MQ=255)
       gTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTAc                                                                                                                                                <  1:916342/150‑1 (MQ=255)
         tCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACgg                                                                                                                                              >  1:3353908/1‑150 (MQ=255)
         tCTGGAAGCCAGTGATAGTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACgg                                                                                                                                              <  1:2028049/150‑1 (MQ=255)
                 ccAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTa                                                                                                                                      >  2:3561501/1‑150 (MQ=255)
                         atTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATc                                                                                                                              >  2:4308079/1‑150 (MQ=255)
                          ttGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATcc                                                                                                                             <  2:225275/150‑1 (MQ=255)
                           tGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCcg                                                                                                                            >  2:2996206/1‑150 (MQ=255)
                           tGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCcg                                                                                                                            >  1:4630031/1‑150 (MQ=255)
                              cgccTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCgcgg                                                                                                                         >  2:1219807/1‑150 (MQ=255)
                               tccTGCGGTGTAGTAGTCGCTTCGAGAACGTACGCGTTTTGATGACCAGTAGGTAATGCCGAGCGTAAACACGTCGATAATCATGAACATAATAATCGCCTGCCAGTTCTTTGGCTTGCGCTCTAAGGCCCCGCTAATAGCATCCgcggc                                                                                                                        <  1:2287144/149‑1 (MQ=255)
                               gccTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCgcggc                                                                                                                        >  2:3292395/1‑150 (MQ=255)
                               gccTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCgcggc                                                                                                                        >  2:3292561/1‑150 (MQ=255)
                                ccTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCgcggcg                                                                                                                       >  2:1746889/1‑150 (MQ=255)
                                ccTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCgcggcg                                                                                                                       <  2:309652/150‑1 (MQ=255)
                                 cTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGt                                                                                                                      >  1:454032/1‑150 (MQ=255)
                                  tGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGtt                                                                                                                     >  1:1365097/1‑150 (MQ=255)
                                     ggTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGc                                                                                                                  <  1:100901/150‑1 (MQ=255)
                                        gtagtaGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGc                                                                                                               >  2:163602/1‑150 (MQ=255)
                                          agtagtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGa                                                                                                             >  1:2184223/1‑150 (MQ=255)
                                          agtagtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGa                                                                                                             >  2:4637247/1‑150 (MQ=255)
                                          agtagtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGa                                                                                                             >  1:591120/1‑150 (MQ=255)
                                           gtagtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGaa                                                                                                            >  1:1802786/1‑150 (MQ=255)
                                           gtagtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGaa                                                                                                            >  1:2353816/1‑150 (MQ=255)
                                           gtagtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGaa                                                                                                            <  2:3359226/150‑1 (MQ=255)
                                             agtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAg                                                                                                          >  2:4426975/1‑150 (MQ=255)
                                              gtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAgg                                                                                                         >  1:2520357/1‑150 (MQ=255)
                                              gtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAgg                                                                                                         >  1:3586144/1‑150 (MQ=255)
                                                  cTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAgt                                                                                                     <  1:3875947/150‑1 (MQ=255)
                                                   tACGAGTACGTACGCGTTTTGATGCCCAGTTGGTACTGCCGAGCGTAAACACGACGAAAATCAGGCACATACTAAGCGCCTGCCCGTTCGGGGGCTGGCGCTCTACGGCCCCGCTCATAGCATCCGCGGCGTGAGCTGCGCCAGGGAgtg                                                                                                    >  2:4561398/1‑150 (MQ=255)
                                                   tACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAgtg                                                                                                    >  2:839229/1‑150 (MQ=255)
                                                      gagaACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTgg                                                                                                 >  1:1162950/1‑150 (MQ=255)
                                                       agaACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTggc                                                                                                >  1:1065030/1‑150 (MQ=255)
                                                        gaACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTggcg                                                                                               >  1:1666109/1‑150 (MQ=255)
                                                           cGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCa                                                                                            <  2:844364/150‑1 (MQ=255)
                                                           cGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCa                                                                                            >  2:4329344/1‑150 (MQ=255)
                                                            gTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCaa                                                                                           <  1:2423240/150‑1 (MQ=255)
                                                            gTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCaa                                                                                           >  2:1228549/1‑150 (MQ=255)
                                                                  gTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCg                                                                                     <  1:548310/150‑1 (MQ=255)
                                                                  gTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCg                                                                                     >  2:1389715/1‑150 (MQ=255)
                                                                     ttGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCa                                                                                  <  1:3211176/150‑1 (MQ=255)
                                                                     ttGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCa                                                                                  >  2:919587/1‑150 (MQ=255)
                                                                      tGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAg                                                                                 <  1:3485274/150‑1 (MQ=255)
                                                                       gATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGa                                                                                <  2:1855382/150‑1 (MQ=255)
                                                                             cAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCtt                                                                          <  2:41617/150‑1 (MQ=255)
                                                                               gTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCtttt                                                                        >  2:4090231/1‑150 (MQ=255)
                                                                                tAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTc                                                                       >  1:2252685/1‑150 (MQ=255)
                                                                                tAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTc                                                                       >  1:4254656/1‑150 (MQ=255)
                                                                                  ggTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCat                                                                     >  1:4019155/1‑150 (MQ=255)
                                                                                     aaTGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCatgat                                                                  >  2:2118137/1‑150 (MQ=255)
                                                                                         ccGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCtt                                                              <  2:2939116/150‑1 (MQ=255)
                                                                                            aGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTa                                                           >  1:3529958/1‑150 (MQ=255)
                                                                                              cGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCGCTACGGCCCCGCTAAGAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTAcc                                                         <  2:1098930/150‑1 (MQ=255)
                                                                                                tAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTc                                                       <  1:2952174/150‑1 (MQ=255)
                                                                                                 aaaCACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCa                                                      >  1:4118692/1‑150 (MQ=255)
                                                                                                   acacGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATg                                                    >  1:4028118/1‑150 (MQ=255)
                                                                                                    cacGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGc                                                   >  2:2325585/1‑150 (MQ=255)
                                                                                                    cacGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGc                                                   >  2:680974/1‑150 (MQ=255)
                                                                                                     acgacgAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCa                                                  <  1:4318/150‑1 (MQ=255)
                                                                                                     acgacgAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCa                                                  >  1:809951/1‑150 (MQ=255)
                                                                                                        acgaAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGa                                               >  2:3674519/1‑150 (MQ=255)
                                                                                                        acgaAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGa                                               >  2:1681159/1‑150 (MQ=255)
                                                                                                         cgaAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGAc                                              <  2:3505869/150‑1 (MQ=255)
                                                                                                          gaAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACt                                             <  1:713536/150‑1 (MQ=255)
                                                                                                            aaaTCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTc                                           >  2:978540/1‑150 (MQ=255)
                                                                                                                cAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCatta                                       >  1:3330810/1‑150 (MQ=255)
                                                                                                                cAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCatta                                       <  2:1414379/150‑1 (MQ=255)
                                                                                                                 aGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCattat                                      >  1:2566620/1‑150 (MQ=255)
                                                                                                                   gAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCAttatta                                    <  2:2667149/150‑1 (MQ=255)
                                                                                                                     aCATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAg                                  <  2:4027558/150‑1 (MQ=255)
                                                                                                                        taataaTCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACg                               <  1:3062506/150‑1 (MQ=255)
                                                                                                                        taataaTCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACg                               >  1:3082680/1‑150 (MQ=255)
                                                                                                                          ataatCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGt                             <  2:1186586/150‑1 (MQ=255)
                                                                                                                             atCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGa                          >  2:1286507/1‑150 (MQ=255)
                                                                                                                             atCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGa                          >  2:3988804/1‑150 (MQ=255)
                                                                                                                                gCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAAtt                       <  1:952808/150‑1 (MQ=255)
                                                                                                                                 ccTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTc                      >  1:1505222/1‑150 (MQ=255)
                                                                                                                                 ccTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTc                      >  2:613526/1‑150 (MQ=255)
                                                                                                                                 ccTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTc                      >  2:4596637/1‑150 (MQ=255)
                                                                                                                                 ccTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTc                      >  1:2114955/1‑150 (MQ=255)
                                                                                                                                 ccTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCAAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGAAGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAAGTc                      >  2:196095/1‑150 (MQ=255)
                                                                                                                                  cTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCg                     >  1:338421/1‑150 (MQ=255)
                                                                                                                                  cTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCg                     >  1:1556735/1‑150 (MQ=255)
                                                                                                                                  cTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCg                     <  1:1650147/150‑1 (MQ=255)
                                                                                                                                  cTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCAGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCg                     <  1:95793/150‑1 (MQ=255)
                                                                                                                                   tGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGc                    >  1:4512615/1‑150 (MQ=255)
                                                                                                                                    gCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAACTCGcc                   <  2:1794272/150‑1 (MQ=255)
                                                                                                                                        gTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTc               <  2:1535650/150‑1 (MQ=255)
                                                                                                                                          tCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgc             <  2:3181577/150‑1 (MQ=255)
                                                                                                                                           cGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgcc            <  1:2824990/150‑1 (MQ=255)
                                                                                                                                            gTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgccc           >  2:1843116/1‑150 (MQ=255)
                                                                                                                                            gTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgccc           >  1:666079/1‑150 (MQ=255)
                                                                                                                                            gTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgccc           >  1:1243854/1‑150 (MQ=255)
                                                                                                                                              tggctggcGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgcccgc         >  2:209106/1‑150 (MQ=255)
                                                                                                                                               ggctggcGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgcccgcc        >  2:2463507/1‑150 (MQ=255)
                                                                                                                                                gctggcGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCa       <  1:1890824/150‑1 (MQ=255)
                                                                                                                                                   ggcGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGAt    <  2:25772/150‑1 (MQ=255)
                                                                                                                                                    gcgcTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATg   <  2:3976373/150‑1 (MQ=255)
                                                                                                                                                     cgcTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGt  <  2:934979/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGT  >  CP009273/4274599‑4274897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑