Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,276,940 G→T 22.2% F120L (TTC→TTA)  acs ← acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,276,9400GT22.2% 810.5 / 169.0 302F120L (TTC→TTA) acsacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (132/103);  new base T (33/34);  total (165/137)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 311 total aligned reads displayed.

GCTTCCGGCGAGAAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTC  >  CP009273/4276791‑4277089
                                                                                                                                                     |                                                                                                                                                     
gCTTCCGGCGAGAAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCg                                                                                                                                                       >  1:4264262/1‑150 (MQ=255)
     cGGCGAGAAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCg                                                                                                                                                  <  1:3043358/150‑1 (MQ=255)
       gCGAGAAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGc                                                                                                                                                <  1:3054082/150‑1 (MQ=255)
        cGAGAAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCa                                                                                                                                               >  2:3090508/1‑150 (MQ=255)
         gagaAGCCGCCGAAAATCAACGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAg                                                                                                                                              >  2:4496056/1‑150 (MQ=255)
          agaAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGa                                                                                                                                             >  2:1043834/1‑150 (MQ=255)
           gaAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGAc                                                                                                                                            >  2:1989186/1‑150 (MQ=255)
            aaGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACg                                                                                                                                           >  1:3326357/1‑150 (MQ=255)
             aGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGt                                                                                                                                          >  2:956899/1‑150 (MQ=255)
                cgccgAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTcgc                                                                                                                                       <  1:3198344/150‑1 (MQ=255)
                 gccgAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTcgcg                                                                                                                                      <  1:25048/150‑1 (MQ=255)
                  ccgAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCgg                                                                                                                                     >  2:3822793/1‑150 (MQ=255)
                   cgAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGt                                                                                                                                    <  2:1088228/150‑1 (MQ=255)
                      aaaTCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCa                                                                                                                                 >  1:3870965/1‑150 (MQ=255)
                      aaaTCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAAGGCCCAGCTCGAGCAGGGTATTGGTGAAGCGGCAGACGTCGCGGTGCa                                                                                                                                 <  1:162901/150‑1 (MQ=255)
                             cGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCttt                                                                                                                          <  2:2386248/150‑1 (MQ=255)
                               aaTGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTtat                                                                                                                        <  2:2667200/150‑1 (MQ=255)
                                aTGCACCGCGCCAATTCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGAACCATCGGCATATAAATCGCCACCACATCACCTTTTTTATTGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCATACGTCGCGGTGCAGCTCTTtata                                                                                                                       >  1:311694/1‑150 (MQ=255)
                                aTGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTtata                                                                                                                       >  2:864064/1‑150 (MQ=255)
                                   cACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCt                                                                                                                    >  2:4159285/1‑150 (MQ=255)
                                    aCCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTg                                                                                                                   >  2:333934/1‑150 (MQ=255)
                                      cgcgCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGat                                                                                                                 <  2:4378681/150‑1 (MQ=255)
                                        cgcCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGTGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGatat                                                                                                               >  1:1234344/1‑150 (MQ=255)
                                           cAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGtt                                                                                                            >  2:2052273/1‑150 (MQ=255)
                                            aaTGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGAGATGttt                                                                                                           >  1:2380282/1‑150 (MQ=255)
                                              tGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGc                                                                                                         >  1:4194729/1‑150 (MQ=255)
                                              tGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGc                                                                                                         >  1:4489722/1‑150 (MQ=255)
                                               gCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGGATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGct                                                                                                        >  2:520935/1‑150 (MQ=255)
                                                  ggCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTctg                                                                                                     <  1:713365/150‑1 (MQ=255)
                                                  ggCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTctg                                                                                                     <  1:4087310/150‑1 (MQ=255)
                                                    cgcAGGCCAGCATCGCATCCTCGGCTTCCGGCACAATCGGCATTTTAATCGCCACAAGATAACCATTTTTAATGCCCATCTCGATGAGGGTATTGTCGTAGCTGCAGACGTCGCGGTGCAGCTCATTATTGCGGATATGTCTTCTctggc                                                                                                   <  2:1169132/150‑1 (MQ=255)
                                                      cAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTctggctg                                                                                                 >  1:4411987/1‑150 (MQ=255)
                                                       aGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTctggctgg                                                                                                >  1:4037875/1‑150 (MQ=255)
                                                       aGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCGGATAGGTTTGCTctggctgg                                                                                                >  1:2710135/1‑150 (MQ=255)
                                                         gCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGcg                                                                                              <  2:4358723/150‑1 (MQ=255)
                                                           cAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGcgtc                                                                                            <  1:215224/150‑1 (MQ=255)
                                                           cAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGcgtc                                                                                            <  2:305789/150‑1 (MQ=255)
                                                            aGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGcgtcg                                                                                           <  1:3879228/150‑1 (MQ=255)
                                                            aGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGcgtcg                                                                                           >  2:122015/1‑150 (MQ=255)
                                                                 cGCAACCGCGTCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGAGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGcc                                                                                      <  1:682694/150‑1 (MQ=255)
                                                                 cGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGcc                                                                                      >  2:3771161/1‑150 (MQ=255)
                                                                   cAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCtt                                                                                    <  1:1095470/150‑1 (MQ=255)
                                                                       cgcgGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCa                                                                                >  1:2325782/1‑150 (MQ=255)
                                                                        gcgGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAg                                                                               <  2:3119186/150‑1 (MQ=255)
                                                                            cTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAgatga                                                                           >  2:2458229/1‑150 (MQ=255)
                                                                                cGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGc                                                                       >  1:4186961/1‑150 (MQ=255)
                                                                                cGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGc                                                                       >  1:4171953/1‑150 (MQ=255)
                                                                                 ggCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCg                                                                      >  2:888097/1‑150 (MQ=255)
                                                                                    aCCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTa                                                                   >  1:4325801/1‑150 (MQ=255)
                                                                                     ccATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTAc                                                                  >  2:428067/1‑150 (MQ=255)
                                                                                         cGGCATATAAATCGCCACCACATCACCTTTTTTAATGTCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATc                                                              <  2:3490786/150‑1 (MQ=255)
                                                                                            cATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGcc                                                           <  1:3785320/150‑1 (MQ=255)
                                                                                            cATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGcc                                                           >  2:3516426/1‑150 (MQ=255)
                                                                                            cATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGcc                                                           <  1:771811/150‑1 (MQ=255)
                                                                                             atatAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCg                                                          >  2:1468150/1‑150 (MQ=255)
                                                                                              tataAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGt                                                         >  2:367133/1‑150 (MQ=255)
                                                                                                taAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGttt                                                       >  1:3703860/1‑150 (MQ=255)
                                                                                                   aTCGCCACCACATCACCTTTTTTAATGCCCCGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCt                                                    >  2:560490/1‑150 (MQ=255)
                                                                                                   aTCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCt                                                    >  2:3686746/1‑150 (MQ=255)
                                                                                                    tCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCtt                                                   >  2:4436189/1‑150 (MQ=255)
                                                                                                    tCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCtt                                                   >  1:2221421/1‑150 (MQ=255)
                                                                                                    tCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCtt                                                   >  2:185849/1‑150 (MQ=255)
                                                                                                     cGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTg                                                  <  1:2623292/150‑1 (MQ=255)
                                                                                                      gCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGc                                                 >  2:2844724/1‑150 (MQ=255)
                                                                                                        caccacATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAg                                               >  1:3463588/1‑150 (MQ=255)
                                                                                                         accacATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGa                                              >  1:1460217/1‑150 (MQ=255)
                                                                                                         accacATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGa                                              <  1:3282442/150‑1 (MQ=255)
                                                                                                          ccacATCACCTTTTTTAATGCCCAGCTCGAGCCGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGAt                                             <  2:2171701/150‑1 (MQ=255)
                                                                                                            acaTCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATgg                                           <  1:3627638/150‑1 (MQ=255)
                                                                                                              aTCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCg                                         <  1:1007512/150‑1 (MQ=255)
                                                                                                              aTCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCg                                         >  2:180012/1‑150 (MQ=255)
                                                                                                                cACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGt                                       >  2:2659036/1‑150 (MQ=255)
                                                                                                                 aCCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTc                                      <  2:3384262/150‑1 (MQ=255)
                                                                                                                 aCCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTc                                      <  1:3528169/150‑1 (MQ=255)
                                                                                                                     tttttAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCATACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTAGTCGCCTTCCCAGATGATGGCGGTACTATCGCAGTTTTCTTGCAGATGGCGGTCAAgg                                  <  1:196027/150‑1 (MQ=255)
                                                                                                                     tttttAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAgg                                  <  1:3242285/150‑1 (MQ=255)
                                                                                                                      ttttAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGTAGATGGCGGTCAAGGc                                 <  1:3656875/150‑1 (MQ=255)
                                                                                                                      ttttAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGc                                 <  1:2154855/150‑1 (MQ=255)
                                                                                                                       tttAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCa                                <  1:2677201/150‑1 (MQ=255)
                                                                                                                       tttAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGACTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCa                                <  1:3084053/150‑1 (MQ=255)
                                                                                                                          aaTGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGtt                             >  1:291580/1‑150 (MQ=255)
                                                                                                                           aTGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGttt                            >  2:2805442/1‑150 (MQ=255)
                                                                                                                            tGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTg                           <  2:904506/150‑1 (MQ=255)
                                                                                                                               ccAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTgccg                        >  2:4070362/1‑150 (MQ=255)
                                                                                                                                      gAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGAtt                 >  2:1591882/1‑150 (MQ=255)
                                                                                                                                      gAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGAtt                 >  1:1887607/1‑150 (MQ=255)
                                                                                                                                      gAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGAtt                 >  1:193656/1‑150 (MQ=255)
                                                                                                                                      gAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGAtt                 <  2:4215865/150‑1 (MQ=255)
                                                                                                                                      gAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGAtt                 >  2:2196550/1‑150 (MQ=255)
                                                                                                                                      gAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGAtt                 <  2:2294046/150‑1 (MQ=255)
                                                                                                                                       aGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTc                >  2:3856307/1‑150 (MQ=255)
                                                                                                                                       aGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTc                >  2:2959453/1‑150 (MQ=255)
                                                                                                                                            ggTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGt           <  1:4366899/150‑1 (MQ=255)
                                                                                                                                             gTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTg          <  2:4568058/150‑1 (MQ=255)
                                                                                                                                               aTTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGcc        <  2:3230666/150‑1 (MQ=255)
                                                                                                                                                ttGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCg       <  1:2803450/150‑1 (MQ=255)
                                                                                                                                                     gAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTc  <  1:3422295/150‑1 (MQ=255)
                                                                                                                                                     gAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTc  <  2:682201/150‑1 (MQ=255)
                                                                                                                                                     gAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTc  >  1:2051732/1‑150 (MQ=255)
                                                                                                                                                     gAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTc  <  1:584509/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTTCCGGCGAGAAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTC  >  CP009273/4276791‑4277089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑