Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,290,080 G→T 100% I256I (ATC→ATA)  mdtP ← outer membrane factor of efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,290,0800GT97.1% 815.6 / 11.7 245I256I (ATC→ATA) mdtPouter membrane factor of efflux pump
Reads supporting (aligned to +/- strand):  ref base G (5/2);  new base T (111/127);  total (116/129)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 245 total aligned reads displayed.

TTGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTC  >  CP009273/4289933‑4290226
                                                                                                                                                   |                                                                                                                                                  
ttGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTAt                                                                                                                                                  <  1:4243014/150‑1 (MQ=255)
   cAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTg                                                                                                                                               <  2:1656665/150‑1 (MQ=255)
     gATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGcc                                                                                                                                             <  2:150065/150‑1 (MQ=255)
     gATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGcc                                                                                                                                             <  1:3374917/150‑1 (MQ=255)
      aTCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGccc                                                                                                                                            <  1:2114104/150‑1 (MQ=255)
          gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTg                                                                                                                                        <  1:1092647/150‑1 (MQ=255)
          gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTg                                                                                                                                        >  2:1523953/1‑150 (MQ=255)
          gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTg                                                                                                                                        <  2:3833789/150‑1 (MQ=255)
          gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTg                                                                                                                                        >  1:1766484/1‑150 (MQ=255)
          gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTg                                                                                                                                        <  2:4591922/150‑1 (MQ=255)
          gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTg                                                                                                                                        >  1:2218117/1‑150 (MQ=255)
           ggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGa                                                                                                                                       <  2:3129909/150‑1 (MQ=255)
            gCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGAc                                                                                                                                      <  1:109402/150‑1 (MQ=255)
            gCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGAc                                                                                                                                      <  1:2806444/150‑1 (MQ=255)
              gTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACgg                                                                                                                                    >  2:1742899/1‑150 (MQ=255)
                 tGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGcaa                                                                                                                                 <  1:55664/150‑1 (MQ=255)
                      aGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAAtt                                                                                                                            <  1:3028259/150‑1 (MQ=255)
                           cTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGttt                                                                                                                       <  2:1825263/150‑1 (MQ=255)
                            tCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTa                                                                                                                      >  1:455848/1‑150 (MQ=255)
                                aaGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTTATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGa                                                                                                                  <  1:1023321/150‑1 (MQ=255)
                                   agagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCg                                                                                                               <  1:3043615/150‑1 (MQ=255)
                                         tCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTTATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGaa                                                                                                         >  1:854843/1‑150 (MQ=255)
                                         tCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGaa                                                                                                         <  1:732444/150‑1 (MQ=255)
                                              ggAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtgt                                                                                                    >  2:3905202/1‑150 (MQ=255)
                                               gAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtgtg                                                                                                   >  2:1705889/1‑150 (MQ=255)
                                               gAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtgtg                                                                                                   >  2:2989818/1‑150 (MQ=255)
                                                 aTGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGcc                                                                                                 >  2:3426148/1‑150 (MQ=255)
                                                 aTGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGcc                                                                                                 >  1:4062534/1‑150 (MQ=255)
                                                    ccGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcgc                                                                                              >  1:1398521/1‑150 (MQ=255)
                                                       gTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGACCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcgcgcg                                                                                           >  2:4143228/1‑150 (MQ=255)
                                                        tCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCgcgcgc                                                                                          >  1:2562995/1‑150 (MQ=255)
                                                             aCTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCACCAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTgg                                                                                     >  2:365613/1‑150 (MQ=255)
                                                             aCTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTgg                                                                                     >  2:2202164/1‑150 (MQ=255)
                                                               ttGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGaa                                                                                   <  2:498629/150‑1 (MQ=255)
                                                                   ggTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGc                                                                               >  2:776281/1‑150 (MQ=255)
                                                                   ggTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGc                                                                               >  2:2569829/1‑150 (MQ=255)
                                                                   ggTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGc                                                                               >  2:161941/1‑150 (MQ=255)
                                                                      aaTGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACt                                                                            >  2:829726/1‑150 (MQ=255)
                                                                      aaTGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCACGCGCGCCGTGGAAAGGCACt                                                                            <  1:2843503/150‑1 (MQ=255)
                                                                       aTGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCTTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACtt                                                                           <  1:4029645/150‑1 (MQ=255)
                                                                       aTGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACtt                                                                           >  2:1943872/1‑150 (MQ=255)
                                                                         gCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTgc                                                                         <  2:4237629/150‑1 (MQ=255)
                                                                         gCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTgc                                                                         <  2:3960735/150‑1 (MQ=255)
                                                                         gCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTgc                                                                         <  2:4118961/150‑1 (MQ=255)
                                                                         gCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTgc                                                                         <  1:2275809/150‑1 (MQ=255)
                                                                           cACCGGTCTTATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGATATTTGCCCTTTGACGGCAACAATTAGATAAGCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTgcgc                                                                       >  2:3465496/1‑150 (MQ=255)
                                                                               ggTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTcc                                                                   <  2:1371457/150‑1 (MQ=255)
                                                                                gTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCa                                                                  >  1:4382961/1‑150 (MQ=255)
                                                                                gTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCa                                                                  <  2:3414442/150‑1 (MQ=255)
                                                                                gTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCa                                                                  <  1:1800/150‑1 (MQ=255)
                                                                                 tCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAg                                                                 <  2:1508325/150‑1 (MQ=255)
                                                                                  cTGATCTCCGGCATCTCGCTGGCTCCAGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACTATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCTCTTGCGCTTCCAGa                                                                <  1:201074/150‑1 (MQ=255)
                                                                                    gATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGAcc                                                              <  2:669301/150‑1 (MQ=255)
                                                                                    gATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGAcc                                                              <  2:412145/150‑1 (MQ=255)
                                                                                    gATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGAcc                                                              <  2:899570/150‑1 (MQ=255)
                                                                                     aTCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCg                                                             <  2:3494829/150‑1 (MQ=255)
                                                                                     aTCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCg                                                             >  1:2661518/1‑150 (MQ=255)
                                                                                      tctcCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGt                                                            <  2:3902379/150‑1 (MQ=255)
                                                                                      tctcCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGt                                                            <  2:4489620/150‑1 (MQ=255)
                                                                                         ccGGCATATCGCAGGCTCCCGCGCCAAACAATGCACGCAGAGATTCTCGAGTTTCGGATATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCCCTTGCGCTTCCAGACCGTgcg                                                         >  2:509522/1‑150 (MQ=255)
                                                                                           ggCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGcc                                                       >  2:400355/1‑150 (MQ=255)
                                                                                                   gCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTAct                                               <  2:2753055/150‑1 (MQ=255)
                                                                                                    cTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTActc                                              >  1:918234/1‑150 (MQ=255)
                                                                                                     tGGCTCCCGCTCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTActct                                             <  2:3032618/150‑1 (MQ=255)
                                                                                                     tGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTActct                                             <  2:141208/150‑1 (MQ=255)
                                                                                                     tGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTActct                                             >  1:1279764/1‑150 (MQ=255)
                                                                                                     tGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCACTTTGACGGCAACAATTTGTTTATAGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTActct                                             <  1:1242310/150‑1 (MQ=255)
                                                                                                     tGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTActct                                             >  1:3260283/1‑150 (MQ=255)
                                                                                                        cTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGt                                          <  1:1454469/150‑1 (MQ=255)
                                                                                                        cTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGt                                          >  1:4128970/1‑150 (MQ=255)
                                                                                                         tCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTg                                         >  2:2117985/1‑150 (MQ=255)
                                                                                                           ccGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTgcg                                       >  1:4625906/1‑150 (MQ=255)
                                                                                                             gcgcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCt                                     >  1:4201429/1‑150 (MQ=255)
                                                                                                              cgcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCtt                                    >  2:3153690/1‑150 (MQ=255)
                                                                                                                 caatcaatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCa                                 <  1:3226303/150‑1 (MQ=255)
                                                                                                                  aatcaatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCAc                                >  2:572963/1‑150 (MQ=255)
                                                                                                                   atcaatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCAcc                               <  1:1082958/150‑1 (MQ=255)
                                                                                                                   atcaatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCAcc                               >  2:1128471/1‑150 (MQ=255)
                                                                                                                   atcaatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCAcc                               <  1:451810/150‑1 (MQ=255)
                                                                                                                     caatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGAGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACcgc                             >  2:3707823/1‑150 (MQ=255)
                                                                                                                      aatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACcgcg                            >  2:3857772/1‑150 (MQ=255)
                                                                                                                      aatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACcgcg                            >  2:1091407/1‑150 (MQ=255)
                                                                                                                        tGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTa                          <  1:3806320/150‑1 (MQ=255)
                                                                                                                         gCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaa                         <  2:4155812/150‑1 (MQ=255)
                                                                                                                         gCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaa                         <  1:3416899/150‑1 (MQ=255)
                                                                                                                          cACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaat                        <  1:2592406/150‑1 (MQ=255)
                                                                                                                            cGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatca                      >  2:3975276/1‑150 (MQ=255)
                                                                                                                            cGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatca                      >  1:690572/1‑150 (MQ=255)
                                                                                                                            cGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCGTTCACCGCGTaatca                      >  1:3772328/1‑150 (MQ=255)
                                                                                                                              cAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatcaat                    >  2:2267204/1‑150 (MQ=255)
                                                                                                                                   aTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACAt               <  2:3788446/150‑1 (MQ=255)
                                                                                                                                   aTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACAt               <  1:1889724/150‑1 (MQ=255)
                                                                                                                                     tctcGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCg             <  1:2647157/150‑1 (MQ=255)
                                                                                                                                     tctcGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCg             <  2:3330391/150‑1 (MQ=255)
                                                                                                                                       tcGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTg           >  1:1838326/1‑150 (MQ=255)
                                                                                                                                           gTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGtt       <  1:2145745/150‑1 (MQ=255)
                                                                                                                                            tttCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGttt      <  2:360555/150‑1 (MQ=255)
                                                                                                                                             ttCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTg     <  1:1679619/150‑1 (MQ=255)
                                                                                                                                                ggTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTc  >  2:4283181/1‑150 (MQ=255)
                                                                                                                                                ggTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCCGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTc  >  1:1129327/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTC  >  CP009273/4289933‑4290226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑