BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,297,485	G→C	90.6%	G44G (GGC→GGG)	alsK ←	D‑allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,297,485	0	G	C	90.6%	1047.7 / 93.2	288	G44G (GGC→GGG)	alsK	D‑allose kinase
Reads supporting (aligned to +/- strand): ref base G (18/9); new base C (125/136); total (143/145)
Fisher's exact test for biased strand distribution p-value = 7.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 290 total aligned reads displayed.

CCGCCGCTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGC  >  CP009273/4297337‑4297631

 cgccgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGccc                                                                                                                                                   >  1:364107/1‑150 (MQ=255)

 cgccgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGcc                                                                                                                                                   <  2:4369150/150‑1 (MQ=255)

       tGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGc                                                                                                                                             >  1:2119521/1‑150 (MQ=255)

          tAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAAt                                                                                                                                          <  2:625062/150‑1 (MQ=255)

          tAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAAt                                                                                                                                          >  2:1095156/1‑150 (MQ=255)

          tAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAAt                                                                                                                                          <  2:1558788/150‑1 (MQ=255)

           aaCGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGACGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTAGCCGATACCCGACACCAGCCCGGGAGCAATg                                                                                                                                         >  2:3353666/1‑150 (MQ=255)

             cGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGAc                                                                                                                                       >  2:4612322/1‑150 (MQ=255)

             cGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGAc                                                                                                                                       >  2:2479422/1‑150 (MQ=255)

              ggCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACt                                                                                                                                      >  1:4103523/1‑150 (MQ=255)

                   gTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGc                                                                                                                                 <  2:1931658/150‑1 (MQ=255)

                   gTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGc                                                                                                                                 >  2:3038776/1‑150 (MQ=255)

                   gTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGc                                                                                                                                 >  1:83107/1‑150 (MQ=255)

                    ttAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCg                                                                                                                                <  1:613570/150‑1 (MQ=255)

                      aGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGt                                                                                                                              <  1:3743638/150‑1 (MQ=255)

                      aGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGt                                                                                                                              >  1:813476/1‑150 (MQ=255)

                        gCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTcc                                                                                                                            <  1:1511840/150‑1 (MQ=255)

                          gTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGc                                                                                                                          >  1:37162/1‑150 (MQ=255)

                            aGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑tt                                                                                                                       <  1:3976712/150‑1 (MQ=255)

                             gAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑ttt                                                                                                                      <  1:1378230/150‑1 (MQ=255)

                              aaaTAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑tttt                                                                                                                     >  2:3956414/1‑150 (MQ=255)

                              aaaTAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑tttt                                                                                                                     >  1:3552027/1‑150 (MQ=255)

                              aaaTAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑tttt                                                                                                                     >  1:556124/1‑150 (MQ=255)

                               aataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑ttttt                                                                                                                    <  2:4399702/150‑1 (MQ=255)

                                ataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑tttttt                                                                                                                   >  2:3711848/1‑150 (MQ=255)

                                ataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑tttttt                                                                                                                   <  2:870959/150‑1 (MQ=255)

                                 taatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑ttttttt                                                                                                                  >  2:1737455/1‑150 (MQ=255)

                                    tGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGc                                                                                                               <  1:276577/150‑1 (MQ=255)

                                     ggTGCGTTTATCTTTAATGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCa                                                                                                              >  2:2783372/1‑150 (MQ=255)

                                      gTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAg                                                                                                             >  2:4243150/1‑150 (MQ=255)

                                          gTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTa                                                                                                         >  2:1872929/1‑150 (MQ=255)

                                          gTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTa                                                                                                         <  1:2175018/150‑1 (MQ=255)

                                          gTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTa                                                                                                         >  2:4192015/1‑150 (MQ=255)

                                           tttATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAg                                                                                                        <  1:318786/150‑1 (MQ=255)

                                              aTCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGt                                                                                                     >  2:1485803/1‑150 (MQ=255)

                                                 tttACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTc                                                                                                  <  1:3339462/150‑1 (MQ=255)

                                                 tttACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTc                                                                                                  <  1:2716447/150‑1 (MQ=255)

                                                  ttACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCa                                                                                                 >  2:3687059/1‑150 (MQ=255)

                                                  ttACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCa                                                                                                 >  1:4244440/1‑150 (MQ=255)

                                                  ttACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCa                                                                                                 >  2:1044022/1‑150 (MQ=255)

                                                   tACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCAc                                                                                                >  2:2065556/1‑150 (MQ=255)

                                                   tACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCAc                                                                                                >  1:2635774/1‑150 (MQ=255)

                                                   tACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCAc                                                                                                <  1:1564909/150‑1 (MQ=255)

                                                    aCTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCAcc                                                                                               <  2:1323719/150‑1 (MQ=255)

                                                     cTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCt                                                                                              <  2:3970339/150‑1 (MQ=255)

                                                       gACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTc                                                                                            <  2:1533605/150‑1 (MQ=255)

                                                        aCCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTct                                                                                           >  1:4155452/1‑150 (MQ=255)

                                                        aCCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTct                                                                                           <  1:2414811/150‑1 (MQ=255)

                                                         ccAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTctg                                                                                          >  2:2603979/1‑150 (MQ=255)

                                                           aGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTctgct                                                                                        <  2:2351576/150‑1 (MQ=255)

                                                           aGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTctgct                                                                                        <  1:1787880/150‑1 (MQ=255)

                                                           aGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTctgct                                                                                        >  1:855654/1‑150 (MQ=255)

                                                               ccGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTcc                                                                                    >  2:4058466/1‑150 (MQ=255)

                                                                cGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCg                                                                                   >  1:1669074/1‑150 (MQ=255)

                                                                cGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCg                                                                                   >  2:3265776/1‑150 (MQ=255)

                                                                  gAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCa                                                                                 <  1:3059705/150‑1 (MQ=255)

                                                                       cccATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACaa                                                                            <  2:3202012/150‑1 (MQ=255)

                                                                               cAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGat                                                                    >  1:1985975/1‑150 (MQ=255)

                                                                               cAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGat                                                                    <  1:526854/150‑1 (MQ=255)

                                                                                aGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGata                                                                   >  2:1975157/1‑150 (MQ=255)

                                                                                          agcgagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGccc                                                         >  2:4530943/1‑150 (MQ=255)

                                                                                          agcgagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGccc                                                         <  2:1850896/150‑1 (MQ=255)

                                                                                           gcgagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGcccc                                                        <  1:1053202/150‑1 (MQ=255)

                                                                                            cgagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGccccc                                                       <  1:3853373/150‑1 (MQ=255)

                                                                                             gagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCa                                                      <  1:4367696/150‑1 (MQ=255)

                                                                                             gagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCa                                                      <  1:2605089/150‑1 (MQ=255)

                                                                                             gagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTGTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGAGTTGCCCCCa                                                      >  2:138792/1‑150 (MQ=255)

                                                                                                 gTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATc                                                  >  2:1319426/1‑150 (MQ=255)

                                                                                                   tAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATcc                                                 >  1:4339561/1‑150 (MQ=255)

                                                                                                    aaaGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCAc                                               >  1:1111023/1‑150 (MQ=255)

                                                                                                     aaGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACg                                              >  2:2400177/1‑150 (MQ=255)

                                                                                                        cgcCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGccc                                           <  2:2837634/150‑1 (MQ=255)

                                                                                                        cgcCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGccc                                           <  2:45453/150‑1 (MQ=255)

                                                                                                          ccTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGc                                         >  2:2798769/1‑150 (MQ=255)

                                                                                                          ccTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGc                                         >  2:2249494/1‑150 (MQ=255)

                                                                                                           cTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCt                                        >  2:407021/1‑150 (MQ=255)

                                                                                                            tGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTa                                       <  2:3643162/150‑1 (MQ=255)

                                                                                                                   cTCGTCAATCATTTCGCCGATACCCGACACCCGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGtt                                <  2:3500927/150‑1 (MQ=255)

                                                                                                                       tCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGc                            >  1:697209/1‑150 (MQ=255)

                                                                                                                        cAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCt                           <  1:1408382/150‑1 (MQ=255)

                                                                                                                        cAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCt                           <  1:1993296/150‑1 (MQ=255)

                                                                                                                         aaTCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTg                          >  1:2877771/1‑150 (MQ=255)

                                                                                                                         aaTCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTg                          >  2:3514938/1‑150 (MQ=255)

                                                                                                                             aTTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGtttt                      <  1:2484052/150‑1 (MQ=255)

                                                                                                                                 cGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCa                  <  2:92458/150‑1 (MQ=255)

                                                                                                                                  gCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCAt                 <  1:2280053/150‑1 (MQ=255)

                                                                                                                                   ccGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATg                >  2:1136626/1‑150 (MQ=255)

                                                                                                                                     gATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAg              <  1:3978503/150‑1 (MQ=255)

                                                                                                                                     gATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAg              >  2:599097/1‑150 (MQ=255)

                                                                                                                                      aTACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAgg             >  1:3452702/1‑150 (MQ=255)

                                                                                                                                       tACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGc            >  2:3422663/1‑150 (MQ=255)

                                                                                                                                       tACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGc            >  1:1323136/1‑150 (MQ=255)

                                                                                                                                       tACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGc            >  2:794756/1‑150 (MQ=255)

                                                                                                                                         cccGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTg          <  1:2931066/150‑1 (MQ=255)

                                                                                                                                            gACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTAc       <  2:3596935/150‑1 (MQ=255)

                                                                                                                                            gACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTAc       <  2:2702871/150‑1 (MQ=255)

                                                                                                                                              cacCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCt     <  1:2197148/150‑1 (MQ=255)

                                                                                                                                               acCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTc    >  2:4417121/1‑150 (MQ=255)

                                                                                                                                                 cAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGc  >  2:2140068/1‑150 (MQ=255)

                                                                                                                                                 cAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGc  >  1:1151842/1‑150 (MQ=255)

CCGCCGCTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGC  >  CP009273/4297337‑4297631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`