Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 362,159 G→T 94.0% I165I (ATC→ATA)  mhpR ← mhp operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273362,1590GT94.0% 708.1 / 32.3 236I165I (ATC→ATA) mhpRmhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (8/7);  new base T (99/122);  total (107/129)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

GGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATT  >  CP009273/362012‑362303
                                                                                                                                                   |                                                                                                                                                
ggCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTAt                                                                                                                                                <  2:4636539/150‑1 (MQ=255)
ggCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAt                                                                                                                                                <  1:2471360/150‑1 (MQ=255)
ggCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAt                                                                                                                                                >  1:4198362/1‑150 (MQ=255)
   gATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTaa                                                                                                                                             <  1:1977269/150‑1 (MQ=255)
     tAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAAtt                                                                                                                                           <  1:1164250/150‑1 (MQ=255)
     tAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAAtt                                                                                                                                           >  1:273683/1‑150 (MQ=255)
        agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCt                                                                                                                                        >  1:1462892/1‑150 (MQ=255)
        agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCt                                                                                                                                        <  2:4486806/150‑1 (MQ=255)
        agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCt                                                                                                                                        <  1:4571018/150‑1 (MQ=255)
         ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCtt                                                                                                                                       >  2:1052950/1‑150 (MQ=255)
          gCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTg                                                                                                                                      <  2:310753/150‑1 (MQ=255)
            gATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCg                                                                                                                                    <  1:785461/150‑1 (MQ=255)
            gATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCGTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTACTTCCTTGCg                                                                                                                                    <  2:2846335/150‑1 (MQ=255)
                 tctcCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCtt                                                                                                                               <  2:445248/150‑1 (MQ=255)
                    cctcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAAGTCCTTGCGGTCTTGtt                                                                                                                            <  2:4613313/150‑1 (MQ=255)
                     ctcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTc                                                                                                                           <  1:3124617/150‑1 (MQ=255)
                       cctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCg                                                                                                                         >  2:23550/1‑150 (MQ=255)
                         tGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCggg                                                                                                                       >  2:1777973/1‑150 (MQ=255)
                         tGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCggg                                                                                                                       <  2:3520443/150‑1 (MQ=255)
                          gATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGc                                                                                                                      >  1:1800488/1‑150 (MQ=255)
                          gATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGc                                                                                                                      >  1:455668/1‑150 (MQ=255)
                           aTCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCa                                                                                                                     >  2:4263592/1‑150 (MQ=255)
                            tCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCaa                                                                                                                    <  2:1456369/150‑1 (MQ=255)
                             cccAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCaaa                                                                                                                   >  2:1302229/1‑150 (MQ=255)
                             cccAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCaaa                                                                                                                   >  1:2665177/1‑150 (MQ=255)
                             cccAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCaaa                                                                                                                   <  1:1284684/150‑1 (MQ=255)
                                   cgcgGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgcca                                                                                                             <  1:2239821/150‑1 (MQ=255)
                                      ggTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcc                                                                                                          >  2:1820449/1‑150 (MQ=255)
                                      ggTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcc                                                                                                          <  1:4149535/150‑1 (MQ=255)
                                      ggTAGTACTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTAAAAGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATATCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagca                                                                                                          >  1:2360546/1‑149 (MQ=255)
                                       gTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcca                                                                                                         >  1:2857233/1‑150 (MQ=255)
                                       gTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcca                                                                                                         <  1:416445/150‑1 (MQ=255)
                                        tAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGccagccag                                                                                                        >  1:1447974/1‑150 (MQ=255)
                                        tAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGccagccag                                                                                                        >  1:2354951/1‑150 (MQ=255)
                                          gTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGt                                                                                                      >  2:3071581/1‑150 (MQ=255)
                                                tCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCGTCCAGCTTTAACGGTTCCCGTGCCAGTTGAGAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGcc                                                                                                <  2:37903/150‑1 (MQ=255)
                                                 ccGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGGTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGccc                                                                                               <  2:4406074/150‑1 (MQ=255)
                                                   gTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGa                                                                                             >  1:3785139/1‑150 (MQ=255)
                                                             ttGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTc                                                                                   >  2:4351976/1‑150 (MQ=255)
                                                             ttGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTc                                                                                   <  1:2139222/150‑1 (MQ=255)
                                                                cgcgcgcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGa                                                                                >  1:4245412/1‑150 (MQ=255)
                                                                    cgcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCg                                                                            >  1:4466244/1‑150 (MQ=255)
                                                                    cgcgcgcCAGAATGGCTCCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCg                                                                            <  1:4262450/150‑1 (MQ=255)
                                                                      cgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGc                                                                          >  2:3881989/1‑150 (MQ=255)
                                                                         gcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCaaa                                                                       >  1:1095806/1‑150 (MQ=255)
                                                                          ccAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAAGTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAAc                                                                      <  1:2320701/150‑1 (MQ=255)
                                                                          ccAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAAc                                                                      <  1:1552973/150‑1 (MQ=255)
                                                                              aaTGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCg                                                                  <  2:2705017/150‑1 (MQ=255)
                                                                                tGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGcc                                                                <  2:4130989/150‑1 (MQ=255)
                                                                                   cTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGa                                                             <  1:870414/150‑1 (MQ=255)
                                                                                    ttCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGAc                                                            >  2:3934406/1‑150 (MQ=255)
                                                                                       cAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCAt                                                         <  2:2756043/150‑1 (MQ=255)
                                                                                         gCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTg                                                       >  1:1971557/1‑150 (MQ=255)
                                                                                          cTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGc                                                      >  1:2459908/1‑150 (MQ=255)
                                                                                               aCGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGt                                                 <  2:1230494/150‑1 (MQ=255)
                                                                                                 ggTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGa                                               <  2:1154776/150‑1 (MQ=255)
                                                                                                 ggTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGa                                               <  2:3557893/150‑1 (MQ=255)
                                                                                                 ggTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGa                                               >  2:2469450/1‑150 (MQ=255)
                                                                                                   ttCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGaaa                                             >  2:2589171/1‑150 (MQ=255)
                                                                                                       cGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGAt                                         <  1:2062937/150‑1 (MQ=255)
                                                                                                        gTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCCAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGACGCGGCAAACGTCGCCCGACCATTGCCCGGTGACAGGATa                                        >  2:3659115/1‑150 (MQ=255)
                                                                                                        gTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATa                                        >  2:3248469/1‑150 (MQ=255)
                                                                                                        gTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATa                                        >  2:4200817/1‑150 (MQ=255)
                                                                                                        gTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAAAATTTCGATTAATTCCTTGCGGTCATGTTCCGGGCAAAAGGCCAGCCATGTCAGGCCCTAGGCGGTTTTCAGAAGAGGCAAACGTCGCCCGAACAATGCCCGGTGAAAGGATa                                        >  2:1872306/1‑150 (MQ=255)
                                                                                                         tGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATaa                                       <  2:3669578/150‑1 (MQ=255)
                                                                                                          gCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAg                                      <  2:564812/150‑1 (MQ=255)
                                                                                                          gCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAg                                      <  2:2138640/150‑1 (MQ=255)
                                                                                                           ccAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGc                                     >  1:699226/1‑150 (MQ=255)
                                                                                                               ttGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCt                                 <  1:1257567/150‑1 (MQ=255)
                                                                                                               ttGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCt                                 <  2:1940716/150‑1 (MQ=255)
                                                                                                                tGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTg                                <  1:1666832/150‑1 (MQ=255)
                                                                                                                 gATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGa                               >  2:2473059/1‑150 (MQ=255)
                                                                                                                   tAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGaaa                             <  2:347953/150‑1 (MQ=255)
                                                                                                                    aGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGaaat                            <  1:3446944/150‑2 (MQ=255)
                                                                                                                        atcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTg                        <  2:1668576/150‑1 (MQ=255)
                                                                                                                        atcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTg                        <  2:2439704/150‑1 (MQ=255)
                                                                                                                         tcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGa                       <  1:1315744/150‑1 (MQ=255)
                                                                                                                           tccGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGGTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGt                     <  1:2954518/149‑1 (MQ=255)
                                                                                                                           aCCGGGGCGGGAGTCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGt                     <  2:2659567/150‑1 (MQ=255)
                                                                                                                           aCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGt                     >  2:2004589/1‑150 (MQ=255)
                                                                                                                           aCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGt                     >  2:2288332/1‑150 (MQ=255)
                                                                                                                           aCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGt                     >  2:2398172/1‑150 (MQ=255)
                                                                                                                           aCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGt                     >  1:3005631/1‑150 (MQ=255)
                                                                                                                            ccGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTg                    <  2:4546389/150‑1 (MQ=255)
                                                                                                                            ccGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTg                    <  1:1842825/150‑1 (MQ=255)
                                                                                                                             cGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTgg                   <  1:2994064/150‑1 (MQ=255)
                                                                                                                             cGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTgg                   <  2:4038099/150‑1 (MQ=255)
                                                                                                                              ggggCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGt                  >  1:3098199/1‑150 (MQ=255)
                                                                                                                              ggggCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGt                  >  1:1121993/1‑150 (MQ=255)
                                                                                                                                 gCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTc               <  2:1476857/150‑1 (MQ=255)
                                                                                                                                 gCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTc               <  2:187017/150‑1 (MQ=255)
                                                                                                                                  cGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAATCGGCTGAAACGGTGAGTGGTTTcg              >  1:3472896/1‑150 (MQ=255)
                                                                                                                                  cGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTcg              <  2:3144354/150‑1 (MQ=255)
                                                                                                                                   gggAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTcgc             >  1:391728/1‑150 (MQ=255)
                                                                                                                                   gggAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTcgc             <  1:949030/150‑1 (MQ=255)
                                                                                                                                   gggAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTcgc             >  2:2505889/1‑150 (MQ=255)
                                                                                                                                     gAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGt           >  2:374521/1‑150 (MQ=255)
                                                                                                                                      aGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTa          >  1:616798/1‑150 (MQ=255)
                                                                                                                                         cTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTacca       >  1:669737/1‑150 (MQ=255)
                                                                                                                                              aTTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCAtt  <  2:531360/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATT  >  CP009273/362012‑362303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑