Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,335,003 A→T 16.9% Y125N (TAC→AAC)  yjdF ← DUF2238 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,335,0030AT16.9% 805.7 / 146.5 301Y125N (TAC→AAC) yjdFDUF2238 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (119/130);  new base T (24/27);  total (144/157)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 302 total aligned reads displayed.

TCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGACTTTCGCGTAGGTGTATTGTCCGC  >  CP009273/4334855‑4335152
                                                                                                                                                    |                                                                                                                                                     
 cccACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACa                                                                                                                                                     <  2:4082170/150‑1 (MQ=255)
     cTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTTCATccc                                                                                                                                                 <  1:4454384/150‑1 (MQ=255)
        gTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCcgcg                                                                                                                                              <  2:4406004/150‑1 (MQ=255)
          cGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCTCCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCcgcgta                                                                                                                                            <  1:3845034/150‑3 (MQ=255)
          cGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGaa                                                                                                                                            >  2:1435510/1‑150 (MQ=255)
           gCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAAc                                                                                                                                           >  1:3589729/1‑150 (MQ=255)
           gCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAAc                                                                                                                                           >  1:79910/1‑150 (MQ=255)
            cccTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACg                                                                                                                                          >  2:2339037/1‑150 (MQ=255)
             ccTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACga                                                                                                                                         >  2:25539/1‑150 (MQ=255)
             ccTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACga                                                                                                                                         >  2:25279/1‑150 (MQ=255)
                     ccGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGACCGAGAATTtct                                                                                                                                 >  2:4220430/1‑150 (MQ=255)
                       gagaAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCg                                                                                                                               >  2:1540586/1‑150 (MQ=255)
                       gagaAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCg                                                                                                                               >  2:796586/1‑150 (MQ=255)
                           aaaTCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGcc                                                                                                                           <  2:4626776/150‑1 (MQ=255)
                            aaTCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGcca                                                                                                                          <  1:3996272/150‑1 (MQ=255)
                            aaTCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGcca                                                                                                                          <  2:3646846/150‑1 (MQ=255)
                              tcatcCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATGTCTCGTGccacc                                                                                                                        >  1:955485/1‑150 (MQ=255)
                                  ccGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGTTTAATTCATCCATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGAACGTCCATCCCGCGAACGAGAATTTCACGTCCCACCAGTg                                                                                                                    >  1:606328/1‑150 (MQ=255)
                                   agCTCCCTGACCCATGGCCTGGTCTGCCCAAAACTCTATTAATTCATAAATCGCGATTATCGCAAGCGCCACGCAGCAGACCAGGAAAGACACCATTTTACGTCAGCTCACGTACATCCCGCGAACTAGAATTTCTCGTGCCACCAGTga                                                                                                                   <  1:3204270/149‑2 (MQ=255)
                                    gCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCa                                                                                                                  <  2:3981301/150‑1 (MQ=255)
                                        ccTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCa                                                                                                              >  2:4235023/1‑150 (MQ=255)
                                         cTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCAc                                                                                                             <  2:4393878/150‑1 (MQ=255)
                                          tGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCAcc                                                                                                            <  2:3697030/150‑1 (MQ=255)
                                          tGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCAcc                                                                                                            <  2:1376931/150‑1 (MQ=255)
                                          tGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCAcc                                                                                                            >  2:3301992/1‑150 (MQ=255)
                                           gACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCa                                                                                                           <  2:1209414/150‑1 (MQ=255)
                                                agtGCCAGCGCTGCCCCCCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGcccc                                                                                                      <  1:185591/148‑1 (MQ=255)
                                                  tGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTg                                                                                                    >  2:1947365/1‑150 (MQ=255)
                                                   gCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTgg                                                                                                   >  1:1572514/1‑150 (MQ=255)
                                                       gcgcTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGaaaa                                                                                               <  1:2851388/150‑1 (MQ=255)
                                                         gcTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAg                                                                                             <  2:3868229/150‑1 (MQ=255)
                                                          cTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGt                                                                                            <  2:3449434/150‑1 (MQ=255)
                                                          cTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGt                                                                                            <  2:3536844/150‑1 (MQ=255)
                                                           tGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTg                                                                                           <  2:302516/150‑1 (MQ=255)
                                                            gCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGc                                                                                          <  2:106599/150‑1 (MQ=255)
                                                            gCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGc                                                                                          <  2:1747554/150‑1 (MQ=255)
                                                              ccaccaCTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGccc                                                                                        >  1:2245030/1‑150 (MQ=255)
                                                              ccaccaCTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGccc                                                                                        >  2:1669228/1‑150 (MQ=255)
                                                               caccacTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGcccc                                                                                       <  2:1586595/150‑1 (MQ=255)
                                                                   acTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCt                                                                                   <  1:1363319/150‑1 (MQ=255)
                                                                   acTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCt                                                                                   <  1:3147085/150‑1 (MQ=255)
                                                                    cTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCtt                                                                                  >  1:4315194/1‑150 (MQ=255)
                                                                      cGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGt                                                                                <  2:2458272/150‑1 (MQ=255)
                                                                       gATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTc                                                                               >  2:3940169/1‑150 (MQ=255)
                                                                        aTTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAANGTGCCCCAGCTTGTCa                                                                              <  2:3170266/150‑1 (MQ=255)
                                                                        aTTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCa                                                                              >  2:765548/1‑150 (MQ=255)
                                                                        aTTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCa                                                                              <  1:3366217/150‑1 (MQ=255)
                                                                            aTTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACg                                                                          <  1:1692519/150‑1 (MQ=255)
                                                                             ttCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACgg                                                                         <  2:3515901/150‑1 (MQ=255)
                                                                                aTACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGAtt                                                                      <  1:1016738/150‑1 (MQ=255)
                                                                                aTACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCA‑TTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTa                                                                     <  1:2005977/150‑1 (MQ=255)
                                                                                   cATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACg                                                                   <  2:88472/150‑1 (MQ=255)
                                                                                   cATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACg                                                                   >  2:1152578/1‑150 (MQ=255)
                                                                                    aTCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGa                                                                  >  1:1475452/1‑150 (MQ=255)
                                                                                       gcgcTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTc                                                               <  1:2968706/150‑1 (MQ=255)
                                                                                          cTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAAc                                                            >  2:1471536/1‑150 (MQ=255)
                                                                                           ttATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAAcc                                                           >  2:3793182/1‑150 (MQ=255)
                                                                                           ttATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAAcc                                                           <  1:3659448/150‑1 (MQ=255)
                                                                                             aTCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAAcccc                                                         <  2:3381455/150‑1 (MQ=255)
                                                                                              tCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCa                                                        >  2:523957/1‑150 (MQ=255)
                                                                                              tCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCa                                                        <  1:4539938/150‑1 (MQ=255)
                                                                                               cGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAg                                                       >  1:66657/1‑150 (MQ=255)
                                                                                                   aGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCAt                                                   >  2:2611530/1‑150 (MQ=255)
                                                                                                     cgcCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTc                                                 <  2:601018/150‑1 (MQ=255)
                                                                                                      gcCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTcc                                                >  1:3925653/1‑150 (MQ=255)
                                                                                                      gcCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAAATTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGCAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTcc                                                >  1:1601912/1‑150 (MQ=255)
                                                                                                         aCGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGc                                             >  1:2529130/1‑150 (MQ=255)
                                                                                                         aCGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGc                                             >  1:2781770/1‑150 (MQ=255)
                                                                                                             agcagACCAGGAACGCCACCATTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCt                                         <  1:1173364/150‑1 (MQ=255)
                                                                                                             agcagACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCt                                         >  1:1960244/1‑150 (MQ=255)
                                                                                                             agcagACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCt                                         >  2:2185080/1‑150 (MQ=255)
                                                                                                             agcagACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCt                                         >  2:4513464/1‑150 (MQ=255)
                                                                                                              gcagACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTc                                        <  1:71087/150‑1 (MQ=255)
                                                                                                               cagACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCa                                       <  1:324232/150‑1 (MQ=255)
                                                                                                               cagACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCa                                       >  2:683870/1‑150 (MQ=255)
                                                                                                                agACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCaa                                      >  2:4432961/1‑150 (MQ=255)
                                                                                                                   ccAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAAc                                   >  2:2599263/1‑150 (MQ=255)
                                                                                                                   ccAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAAc                                   <  2:1900684/150‑1 (MQ=255)
                                                                                                                     aGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCa                                 <  1:544563/150‑1 (MQ=255)
                                                                                                                     aGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCa                                 >  2:2071585/1‑150 (MQ=255)
                                                                                                                      ggAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCaa                                >  2:2087967/1‑150 (MQ=255)
                                                                                                                      ggAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCaa                                >  2:4254422/1‑150 (MQ=255)
                                                                                                                       gAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAAc                               <  1:3641298/150‑1 (MQ=255)
                                                                                                                        aaCGCCACCATTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACg                              >  1:842576/1‑150 (MQ=255)
                                                                                                                          cGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACggg                            <  2:2844614/150‑1 (MQ=255)
                                                                                                                           gCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACgggg                           <  1:4515053/150‑1 (MQ=255)
                                                                                                                           gCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACgggg                           <  1:205075/150‑1 (MQ=255)
                                                                                                                            ccaccaTTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGa                          >  2:4205603/1‑150 (MQ=255)
                                                                                                                               ccaTTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGACtt                       <  1:2373083/150‑1 (MQ=255)
                                                                                                                               ccaTTTTACGTCCGCGCACGTTCATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGACtt                       <  1:1419005/150‑1 (MQ=255)
                                                                                                                                 aTTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGACTTTc                     <  2:1797875/150‑1 (MQ=255)
                                                                                                                                  ttttACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGACTTTcg                    <  1:1537101/150‑1 (MQ=255)
                                                                                                                                  ttttACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGACTTTcg                    <  2:2968355/150‑1 (MQ=255)
                                                                                                                                  ttttACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGACTTTcg                    <  2:2680036/150‑1 (MQ=255)
                                                                                                                                    ttACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGACTTTcgcg                  <  1:1947365/150‑1 (MQ=255)
                                                                                                                                       cGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGACTTTCGCGTAg               <  1:2922605/150‑1 (MQ=255)
                                                                                                                                        gTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGACTTTCGCGTAgg              <  1:2308030/150‑1 (MQ=255)
                                                                                                                                               cACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGACTTTCGCGTAGGTGTATTg       <  1:2434658/150‑1 (MQ=255)
                                                                                                                                                aCGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGACTTTCGCGTAGGTGTATTGt      >  1:4365058/1‑150 (MQ=255)
                                                                                                                                                 cGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGACTTTCGCGTAGGTGTATTGTc     <  1:1779274/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCGCACGTACATCCCGCGAACGAGAATTTCTCGTGCCACCAGTGCAGGCACCAGCCCCTGGAAAAAGTGCCCCAGCTTGTCATACGGATTACGACTCAACCCCAGCCATTCCTGCACCTCAAAACCAACGGGGACTTTCGCGTAGGTGTATTGTCCGC  >  CP009273/4334855‑4335152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑