Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,394,391 G→T 6.0% T63T (ACG→ACT)  yjeT → DUF2065 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,394,3910GT 6.0% 873.4 / 20.1 229T63T (ACG→ACT) yjeTDUF2065 family protein
Reads supporting (aligned to +/- strand):  ref base G (97/117);  new base T (5/10);  total (102/127)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

GTACTGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATGGG  >  CP009273/4394242‑4394540
                                                                                                                                                     |                                                                                                                                                     
gTACTGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACg                                                                                                                                                       <  1:1094448/150‑1 (MQ=255)
   cTGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGAtt                                                                                                                                                    <  1:459136/150‑1 (MQ=255)
    tGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTg                                                                                                                                                   <  1:3534241/150‑1 (MQ=255)
      gAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGc                                                                                                                                                 <  1:526523/150‑1 (MQ=255)
       aaGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCt                                                                                                                                                >  1:628944/1‑150 (MQ=255)
            ttAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACa                                                                                                                                           >  2:260667/1‑150 (MQ=255)
             tAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACaa                                                                                                                                          >  2:886563/1‑150 (MQ=255)
               gggCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACaaaa                                                                                                                                        >  2:903206/1‑150 (MQ=255)
               gggCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACaaaa                                                                                                                                        >  2:2303945/1‑150 (MQ=255)
                ggCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACaaaaa                                                                                                                                       >  1:2035628/1‑150 (MQ=255)
                   cGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACa                                                                                                                                    <  1:1281408/150‑1 (MQ=255)
                    gATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAg                                                                                                                                   >  2:1987802/1‑150 (MQ=255)
                      tGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGAc                                                                                                                                 <  1:4457248/150‑1 (MQ=255)
                      tGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGAc                                                                                                                                 <  2:2397038/150‑1 (MQ=255)
                       gCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACt                                                                                                                                <  2:3616467/150‑1 (MQ=255)
                       gCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACt                                                                                                                                <  2:1304940/150‑1 (MQ=255)
                        cTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTg                                                                                                                               <  1:792890/150‑1 (MQ=255)
                         tttACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGa                                                                                                                              >  1:1719974/1‑150 (MQ=255)
                           tACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATc                                                                                                                            <  1:3981505/150‑1 (MQ=255)
                            aCCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCg                                                                                                                           <  2:1239136/150‑1 (MQ=255)
                            aCCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCg                                                                                                                           >  2:3284059/1‑150 (MQ=255)
                               cGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAgg                                                                                                                        >  1:4589728/1‑150 (MQ=255)
                                gAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGt                                                                                                                       <  2:204786/150‑1 (MQ=255)
                                      aTGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCAtttt                                                                                                                 >  1:266257/1‑150 (MQ=255)
                                       tGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTAGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCAttttt                                                                                                                >  2:3400819/1‑150 (MQ=255)
                                          aagaagATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAg                                                                                                             >  2:3743048/1‑150 (MQ=255)
                                          aagaagATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAg                                                                                                             <  1:4396606/150‑1 (MQ=255)
                                            gaagaTGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTg                                                                                                           <  1:2552234/150‑1 (MQ=255)
                                             aagaTGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGTGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGc                                                                                                          >  1:3464504/1‑150 (MQ=255)
                                             aagaTGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGTGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGc                                                                                                          >  1:1144605/1‑150 (MQ=255)
                                             aagaTGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGc                                                                                                          >  1:647957/1‑150 (MQ=255)
                                             aagaTGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGc                                                                                                          <  2:2496571/150‑1 (MQ=255)
                                                    tctcTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGt                                                                                                   <  2:1501954/150‑1 (MQ=255)
                                                     ctctGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTg                                                                                                  <  2:470407/150‑1 (MQ=255)
                                                         gCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGt                                                                                              <  2:4426069/150‑1 (MQ=255)
                                                           gATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGACATTTTTGAGTGCAAAAAGTGCTGTaa                                                                                            >  2:3174756/1‑150 (MQ=255)
                                                              gACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAActc                                                                                         >  1:4352671/1‑150 (MQ=255)
                                                               aCCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAActct                                                                                        <  1:2236820/150‑1 (MQ=255)
                                                                ccAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTg                                                                                       <  2:3108761/150‑1 (MQ=255)
                                                                 cAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGa                                                                                      <  1:1648631/150‑1 (MQ=255)
                                                                  aaTTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGaa                                                                                     >  1:2348171/1‑150 (MQ=255)
                                                                   aTTTGCCCTATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGaaa                                                                                    >  1:4234780/1‑150 (MQ=255)
                                                                   aTTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGaaa                                                                                    <  2:2517804/150‑1 (MQ=255)
                                                                    tttGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGaaaa                                                                                   >  2:2556157/1‑150 (MQ=255)
                                                                     ttGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGaaaaa                                                                                  >  2:1607469/1‑150 (MQ=255)
                                                                       gCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGc                                                                                <  1:659443/150‑1 (MQ=255)
                                                                        cccGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCg                                                                               >  1:1201133/1‑150 (MQ=255)
                                                                         ccGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGa                                                                              >  1:1385596/1‑150 (MQ=255)
                                                                          cGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGAt                                                                             <  2:769203/150‑1 (MQ=255)
                                                                             taataTTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGt                                                                          <  1:4502857/150‑1 (MQ=255)
                                                                                tatTTTACGTCGTTTTGGCGGTGGCCTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGa                                                                       <  1:1845298/150‑1 (MQ=255)
                                                                                tatTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGa                                                                       >  2:2618549/1‑150 (MQ=255)
                                                                                 atTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTCGaa                                                                      >  2:4362963/1‑150 (MQ=255)
                                                                                    ttACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATcc                                                                   <  1:4117526/150‑1 (MQ=255)
                                                                                    ttACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATcc                                                                   <  1:319626/150‑1 (MQ=255)
                                                                                    ttACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATcc                                                                   >  2:4174481/1‑150 (MQ=255)
                                                                                     tACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCa                                                                  >  2:3020607/1‑150 (MQ=255)
                                                                                         tcgtTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCAtttt                                                              <  2:642699/150‑1 (MQ=255)
                                                                                         tcgtTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCAtttt                                                              <  2:4131235/150‑1 (MQ=255)
                                                                                          cgtTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCAttttt                                                             <  2:722286/150‑1 (MQ=255)
                                                                                          cgtTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCAttttt                                                             >  2:1845596/1‑150 (MQ=255)
                                                                                            ttttGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTaa                                                           <  2:2575611/150‑1 (MQ=255)
                                                                                              ttGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGc                                                         >  1:3802224/1‑150 (MQ=255)
                                                                                                   ggTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTTCATGTTGAGGAAAACGATTGGCTGAACAAAATACAGACTGATCGAGGTCATTTTTGAGTGCAAAATGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACg                                                    <  2:4460432/150‑1 (MQ=255)
                                                                                                       gACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGa                                                <  2:571106/150‑1 (MQ=255)
                                                                                                        aCTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGAt                                               >  2:3483592/1‑150 (MQ=255)
                                                                                                         cTTGTGTTTGCGGGCGTTGTGGTCTACTTCATGTTGATGTAAAATATTGGCTGAACTAAAATCATTCTGCTCGATGTCTTTTTTGTGTGCAAATTGTGATGTATCTCTGAAAAAGCTATTGTATATTCCATTTTTAATCAAACGTTGttt                                              <  2:1215416/150‑1 (MQ=255)
                                                                                                           tgtgGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGAtttt                                            <  1:309875/150‑1 (MQ=255)
                                                                                                            gtgGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTg                                           <  2:3595825/150‑1 (MQ=255)
                                                                                                               gTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGaaa                                        <  1:335158/150‑1 (MQ=255)
                                                                                                                  gCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAAt                                     <  2:131007/150‑1 (MQ=255)
                                                                                                                    gggCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATgg                                   >  1:3254102/1‑150 (MQ=255)
                                                                                                                    gggCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATgg                                   <  2:3973768/150‑1 (MQ=255)
                                                                                                                     ggCGTTGTGGTCTTCTACATGTTGAGGAAATCGTTTGTCTTAACATATAACAGACTGTTCGAGTTCATTTTTTCGTGGATAAATTGCTGTATATATGACATATCGATGGTTGTATCCTTTTTTTAGCAAACGGTGATTTTGAAAAATgtg                                  <  2:3324160/150‑3 (MQ=255)
                                                                                                                        gTTGTGGTCTACTACATGTTGATGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGATAAATGGGTaa                               <  2:3234485/150‑1 (MQ=255)
                                                                                                                        gTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTaa                               >  2:2122854/1‑150 (MQ=255)
                                                                                                                          tgtgGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTaaca                             >  2:1647658/1‑150 (MQ=255)
                                                                                                                           gtgGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTaacaa                            <  2:1319993/150‑1 (MQ=255)
                                                                                                                            tgGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTaacaac                           <  2:976768/150‑1 (MQ=255)
                                                                                                                             ggTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcg                          >  1:2619719/1‑150 (MQ=255)
                                                                                                                             ggTCCACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACACcg                          <  1:2860297/150‑1 (MQ=255)
                                                                                                                               tCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcgtc                        <  1:805366/150‑1 (MQ=255)
                                                                                                                                 tactacATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGGGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcgtcgt                      >  2:535029/1‑150 (MQ=255)
                                                                                                                                 tactacATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGGGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcgtcgt                      >  2:534331/1‑150 (MQ=255)
                                                                                                                                 tactacATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcgtcgt                      <  2:3668799/150‑1 (MQ=255)
                                                                                                                                  actacATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcgtcgtc                     <  1:564808/150‑1 (MQ=255)
                                                                                                                                   ctacATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcgtcgtcg                    <  1:1504123/150‑1 (MQ=255)
                                                                                                                                      cATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTAc                 <  1:123159/150‑1 (MQ=255)
                                                                                                                                       aTGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACt                <  2:4530799/150‑1 (MQ=255)
                                                                                                                                       aTGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACt                >  2:2865059/1‑150 (MQ=255)
                                                                                                                                        tGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTg               <  2:4066620/150‑1 (MQ=255)
                                                                                                                                            gAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCa           >  2:2019416/1‑150 (MQ=255)
                                                                                                                                               gAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCAccc        >  2:303460/1‑150 (MQ=255)
                                                                                                                                               gAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCAccc        >  2:303231/1‑150 (MQ=255)
                                                                                                                                                  aaCTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAAt     >  1:765650/1‑150 (MQ=255)
                                                                                                                                                   aCGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCCATg    >  2:2773403/1‑150 (MQ=255)
                                                                                                                                                    cGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATgg   >  1:3006763/1‑150 (MQ=255)
                                                                                                                                                     gATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATggg  >  2:4574525/1‑150 (MQ=255)
                                                                                                                                                     gATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATggg  <  2:2184126/150‑1 (MQ=255)
                                                                                                                                                     gATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATggg  <  1:1768086/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTACTGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATGGG  >  CP009273/4394242‑4394540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑