Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,396,598 A→T 100% E43V (GAA→GTA)  rnr → exoribonuclease R, RNase R

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,396,5980AT97.8% 976.1 / 7.5 279E43V (GAA→GTA) rnrexoribonuclease R, RNase R
Reads supporting (aligned to +/- strand):  ref base A (2/5);  new base T (159/113);  total (161/118)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 279 total aligned reads displayed.

ATGACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCC  >  CP009273/4396451‑4396744
                                                                                                                                                   |                                                                                                                                                  
 tGACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAAGCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACt                                                                                                                                                 <  1:2990449/150‑1 (MQ=255)
  gACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGACTTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTg                                                                                                                                                >  2:1443095/1‑150 (MQ=255)
  gACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGACTTTATCCTCGAACATTTAACCAAACGGGAAAAACCGGCCAGCCGGGATGAGCTGGCGGTCGTACTg                                                                                                                                                >  2:2340909/1‑150 (MQ=255)
  gACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTg                                                                                                                                                >  2:1261206/1‑150 (MQ=255)
     aaCGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGACTTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGcac                                                                                                                                             >  1:4331021/1‑150 (MQ=255)
     aaCGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGcac                                                                                                                                             >  2:1425/1‑150 (MQ=255)
        ggaggaACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACAtt                                                                                                                                          <  1:2482541/150‑1 (MQ=255)
        ggaggaACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACAtt                                                                                                                                          <  2:1128713/150‑1 (MQ=255)
         gaggaACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTg                                                                                                                                         >  1:258093/1‑150 (MQ=255)
         gaggaACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTg                                                                                                                                         >  1:3657089/1‑150 (MQ=255)
            gaACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAg                                                                                                                                      >  1:35773/1‑150 (MQ=255)
               ccGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCg                                                                                                                                   <  2:881307/150‑1 (MQ=255)
                 gagaTGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGaa                                                                                                                                 >  2:644019/1‑150 (MQ=255)
                  agaTGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAag                                                                                                                                >  2:3093297/1‑150 (MQ=255)
                        cacaAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGagcagc                                                                                                                          >  1:1383497/1‑150 (MQ=255)
                         acaAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCt                                                                                                                         >  1:980851/1‑150 (MQ=255)
                         acaAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCt                                                                                                                         >  2:67816/1‑150 (MQ=255)
                          caAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCtt                                                                                                                        <  2:4561701/150‑1 (MQ=255)
                              aTCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAg                                                                                                                    >  2:1845948/1‑150 (MQ=255)
                                    tCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGc                                                                                                              <  1:1707171/150‑1 (MQ=255)
                                       aGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTc                                                                                                           >  1:3404370/1‑150 (MQ=255)
                                          aaCGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgcc                                                                                                        >  1:3502091/1‑150 (MQ=255)
                                          aaCGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgcc                                                                                                        >  2:1753174/1‑150 (MQ=255)
                                             gcgAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCgccgcc                                                                                                     >  1:450083/1‑150 (MQ=255)
                                             gcgAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCgccgcc                                                                                                     <  2:3831461/150‑1 (MQ=255)
                                              cgAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCt                                                                                                    >  1:3429960/1‑150 (MQ=255)
                                               gAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTg                                                                                                   <  2:986040/150‑1 (MQ=255)
                                                  gCTGAAAAATACGCGCATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgcgc                                                                                                <  2:2455012/150‑1 (MQ=255)
                                                    tGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgcgcgc                                                                                              >  1:150208/1‑150 (MQ=255)
                                                        aaaTACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATg                                                                                          <  1:421593/150‑1 (MQ=255)
                                                             cgcgAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAgcg                                                                                     <  2:2633905/150‑1 (MQ=255)
                                                              gcgAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTGGACCTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGAGGGAgcgc                                                                                    >  2:2612777/1‑150 (MQ=255)
                                                                gAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGa                                                                                  >  1:2957691/1‑150 (MQ=255)
                                                                gAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGa                                                                                  <  1:2065852/150‑1 (MQ=255)
                                                                gAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGa                                                                                  >  1:720497/1‑150 (MQ=255)
                                                                 aaTCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGAt                                                                                 <  1:877949/150‑1 (MQ=255)
                                                                 aaTCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGAt                                                                                 <  1:3940465/150‑1 (MQ=255)
                                                                      catcccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCa                                                                            >  2:4407061/1‑150 (MQ=255)
                                                                       atcccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCaa                                                                           <  1:881032/150‑1 (MQ=255)
                                                                       atcccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCaa                                                                           >  1:1321494/1‑150 (MQ=255)
                                                                        tcccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAAc                                                                          >  2:2935553/1‑150 (MQ=255)
                                                                         cccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACt                                                                         <  2:3889974/150‑1 (MQ=255)
                                                                          ccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTg                                                                        >  1:3760039/1‑150 (MQ=255)
                                                                           cTAGTCGGGAATTTATCCTCGAACATTGACCCAAACGTGAAAAACCGGCCAGCCGTGAGGAGCGGGCGGTAGTACTGCACATTGAAGGCGTAGAGCAGCTTGAAGGCGTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTgg                                                                       <  2:819272/150‑1 (MQ=255)
                                                                              gTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCt                                                                    >  1:3865653/1‑150 (MQ=255)
                                                                              gTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCt                                                                    >  1:2030080/1‑150 (MQ=255)
                                                                               tCGGGAATTTATCCTCTAACATTTTACCAAACGTGAAAAACAGGCCAGCCGTGATGAGCTGGCGTTATTACTGCACATTGAAGGCTAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGTAGCGCGATGGTCAACTGGTCtt                                                                   <  1:3657236/150‑1 (MQ=255)
                                                                               tCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCtt                                                                   <  1:4009651/150‑1 (MQ=255)
                                                                                cGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTc                                                                  >  1:3507943/1‑150 (MQ=255)
                                                                                cGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTc                                                                  <  1:2613625/150‑1 (MQ=255)
                                                                                cGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTc                                                                  <  2:2600101/150‑1 (MQ=255)
                                                                                 gggAATTTATCCTCGAACATTTAACCAACCGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCa                                                                 <  1:2214106/150‑1 (MQ=255)
                                                                                 gggAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCa                                                                 >  2:130378/1‑150 (MQ=255)
                                                                                 gggAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCa                                                                 >  1:2324596/1‑150 (MQ=255)
                                                                                   gAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACt                                                               >  1:273506/1‑150 (MQ=255)
                                                                                       ttATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACtcgtc                                                           >  2:3385066/1‑150 (MQ=255)
                                                                                          tCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCgtcgtc                                                        <  2:1934530/150‑1 (MQ=255)
                                                                                          tCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCgtcgtc                                                        >  2:3598393/1‑150 (MQ=255)
                                                                                            cTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAg                                                      >  2:2138793/1‑150 (MQ=255)
                                                                                             tCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGt                                                     <  2:3738388/150‑1 (MQ=255)
                                                                                               gAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGc                                                   >  1:961085/1‑150 (MQ=255)
                                                                                                aaCATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCt                                                  >  2:2245708/1‑150 (MQ=255)
                                                                                                aaCATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCt                                                  <  2:3990160/150‑1 (MQ=255)
                                                                                                aaCATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCt                                                  <  1:2707662/150‑1 (MQ=255)
                                                                                                  cATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTAt                                                >  2:2070082/1‑150 (MQ=255)
                                                                                                  cATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTAt                                                <  1:2282161/150‑1 (MQ=255)
                                                                                                   aTTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATg                                               >  1:526005/1‑150 (MQ=255)
                                                                                                     ttAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATgcg                                             <  2:3171221/150‑1 (MQ=255)
                                                                                                       aaCCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCt                                           >  1:488016/1‑150 (MQ=255)
                                                                                                       aaCCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCt                                           >  1:3447849/1‑150 (MQ=255)
                                                                                                       aaCCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCt                                           >  1:4110076/1‑150 (MQ=255)
                                                                                                        aCCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTg                                          >  1:2158938/1‑150 (MQ=255)
                                                                                                          cAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGcc                                        >  1:2810190/1‑150 (MQ=255)
                                                                                                            aaCGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTAACTCGTCGTCAGTGCTATGCGCTGACgg                                      >  2:242000/1‑150 (MQ=255)
                                                                                                             aCGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGa                                     <  2:3679713/150‑1 (MQ=255)
                                                                                                                tGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACg                                  <  1:4605611/150‑1 (MQ=255)
                                                                                                                    aaaCCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTc                              <  2:4337/150‑1 (MQ=255)
                                                                                                                    aaaCCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTc                              <  2:230093/150‑1 (MQ=255)
                                                                                                                     aaCCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCg                             <  2:1350094/150‑1 (MQ=255)
                                                                                                                      aCCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGa                            >  2:4624431/1‑150 (MQ=255)
                                                                                                                        cGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGAcc                          <  1:1580156/150‑1 (MQ=255)
                                                                                                                        cGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGAcc                          >  2:2268407/1‑150 (MQ=255)
                                                                                                                             aGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTg                     <  1:1845948/150‑1 (MQ=255)
                                                                                                                             aGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTg                     <  1:2771062/150‑1 (MQ=255)
                                                                                                                             aGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTg                     >  1:2420694/1‑150 (MQ=255)
                                                                                                                             aGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTg                     <  2:4191690/150‑1 (MQ=255)
                                                                                                                             aGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTg                     >  2:1634487/1‑150 (MQ=255)
                                                                                                                                cGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGaaa                  >  1:3294276/1‑150 (MQ=255)
                                                                                                                                 gTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGACTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAg                 >  1:1020276/1‑150 (MQ=255)
                                                                                                                                   gatgaGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGt               <  1:924653/150‑1 (MQ=255)
                                                                                                                                    atgaGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTa              >  2:736011/1‑150 (MQ=255)
                                                                                                                                    atgaGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTa              <  1:3044733/150‑1 (MQ=255)
                                                                                                                                      gaGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGCTGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTAcc            <  2:590185/150‑1 (MQ=255)
                                                                                                                                      gaGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTAcc            >  1:1996736/1‑150 (MQ=255)
                                                                                                                                       aGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCg           >  1:4380191/1‑150 (MQ=255)
                                                                                                                                         cTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGtt         <  1:1258549/150‑1 (MQ=255)
                                                                                                                                           ggCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTAt       <  1:4557072/150‑1 (MQ=255)
                                                                                                                                           ggCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTAt       >  1:1895016/1‑150 (MQ=255)
                                                                                                                                           ggCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTAt       >  1:2530095/1‑150 (MQ=255)
                                                                                                                                                tAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTAGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGACAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGcc  >  1:1106784/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCC  >  CP009273/4396451‑4396744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑