Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,399,304 C→A 13.9% I71I (ATC→ATA)  rlmB → 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,399,3040CA13.9% 974.4 / 85.6 286I71I (ATC→ATA) rlmB23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (129/117);  new base A (22/18);  total (151/135)
Fisher's exact test for biased strand distribution p-value = 8.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

TTTCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGC  >  CP009273/4399155‑4399451
                                                                                                                                                     |                                                                                                                                                   
tttCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATc                                                                                                                                                     >  2:1795103/1‑150 (MQ=255)
 ttCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCg                                                                                                                                                    >  1:1285726/1‑150 (MQ=255)
 ttCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCg                                                                                                                                                    >  1:2029992/1‑150 (MQ=255)
    aGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGccc                                                                                                                                                 >  2:1375863/1‑150 (MQ=255)
       aaGTCTTTATTTTAAAAGGCCGTGAATATAACCGTCTTTTACCGCTTATTCACGCCCTTGTGTCTCCGGGCGTGGTTATCCAGTGGGCAAACCGCAAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCcgcg                                                                                                                                              <  1:3930931/150‑1 (MQ=255)
       aaGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCcgcg                                                                                                                                              <  2:4019691/150‑1 (MQ=255)
            tttATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAg                                                                                                                                         <  2:232624/150‑1 (MQ=255)
              tATTTTAAAAGGCCGTTAATAAAACCGTCTGTTACCGCTTATTCACGCCCTTGAGTCTCTGGGCGTGGTTACCCAGTTGGCAACCCGCCAATATCCCCACGACAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGcc                                                                                                                                       <  1:776136/150‑1 (MQ=255)
               aTTTTAAAAGGCCGTGAAGATAACCTTCTGTTACCGCTGATTCCCGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAACCCGCCAATATCCCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCa                                                                                                                                      <  1:925614/150‑1 (MQ=255)
               aTTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCa                                                                                                                                      >  1:590346/1‑150 (MQ=255)
                ttttAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAg                                                                                                                                     <  2:1536969/150‑1 (MQ=255)
                ttttAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAg                                                                                                                                     <  1:1904536/150‑1 (MQ=255)
                   tAAACGGCCGTGAATATAACCGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCCGGGCGTGTTTATCCTGTTGGCAAACCGCAAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGAc                                                                                                                                  <  1:4349842/150‑1 (MQ=255)
                        ggCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAg                                                                                                                             <  2:806836/150‑1 (MQ=255)
                        ggCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAg                                                                                                                             >  2:4281227/1‑150 (MQ=255)
                          ccGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCTGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTa                                                                                                                           <  1:2576984/150‑1 (MQ=255)
                            gTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATc                                                                                                                         >  1:3847567/1‑150 (MQ=255)
                            gTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATc                                                                                                                         >  2:1709615/1‑150 (MQ=255)
                              gAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAg                                                                                                                       <  2:111559/150‑1 (MQ=255)
                                aGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGa                                                                                                                     >  1:680247/1‑150 (MQ=255)
                                  aTAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGaaa                                                                                                                   >  1:3769692/1‑150 (MQ=255)
                                    aaaCGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAAc                                                                                                                 >  1:2455748/1‑150 (MQ=255)
                                    aaaCGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAAc                                                                                                                 >  1:2652213/1‑150 (MQ=255)
                                    aaaCGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAAc                                                                                                                 >  1:3593120/1‑150 (MQ=255)
                                     aaCGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACg                                                                                                                >  1:2358450/1‑150 (MQ=255)
                                      aCGTCTGTTACCGCATATTCACGCCCTTGAGTCTCCGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGa                                                                                                               <  1:1972590/150‑1 (MQ=255)
                                       cGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGTTTATCCAGTGGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGAt                                                                                                              <  2:873378/150‑1 (MQ=255)
                                        gTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATc                                                                                                             >  1:918495/1‑150 (MQ=255)
                                          cTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTg                                                                                                           >  1:3991996/1‑150 (MQ=255)
                                           tGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGc                                                                                                          <  2:3164348/150‑1 (MQ=255)
                                             ttACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCg                                                                                                        <  2:1270552/150‑1 (MQ=255)
                                             ttACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCg                                                                                                        >  1:4392707/1‑150 (MQ=255)
                                                  gCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCt                                                                                                   >  1:853955/1‑150 (MQ=255)
                                                    tGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGa                                                                                                 <  2:3012650/150‑1 (MQ=255)
                                                    tGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGa                                                                                                 >  1:1513098/1‑150 (MQ=255)
                                                           cGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGAtcgcttc                                                                                          <  2:2927230/150‑1 (MQ=255)
                                                           cGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGAtcgcttc                                                                                          <  2:3833123/150‑1 (MQ=255)
                                                            gCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGAtcgcttcg                                                                                         >  2:2451465/1‑150 (MQ=255)
                                                             cccTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGAtcgcttcgc                                                                                        >  2:2219786/1‑150 (MQ=255)
                                                              ccTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGAtcgcttcgct                                                                                       <  2:2755598/150‑1 (MQ=255)
                                                                 tGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGa                                                                                    <  2:3250339/150‑1 (MQ=255)
                                                                  gAGTCTCCGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGAt                                                                                   <  1:349661/150‑1 (MQ=255)
                                                                  gAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGAt                                                                                   <  1:3827516/150‑1 (MQ=255)
                                                                      ctcAGGGCGTGGTTATCCTGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAAc                                                                               <  1:4106648/150‑1 (MQ=255)
                                                                        cAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCg                                                                             <  1:2876562/150‑1 (MQ=255)
                                                                         aGGGCGTGTTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGt                                                                            >  1:2766941/1‑150 (MQ=255)
                                                                         aGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGt                                                                            >  1:3043157/1‑150 (MQ=255)
                                                                         aGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGt                                                                            >  1:1615837/1‑150 (MQ=255)
                                                                              gTGGTTATCCTGTTGGCAAACCGCCAATATCCCTACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCctg                                                                       <  1:800278/150‑1 (MQ=255)
                                                                              gTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCctg                                                                       <  2:2359506/150‑1 (MQ=255)
                                                                               tGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCctgc                                                                      >  2:3956888/1‑150 (MQ=255)
                                                                               tGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCctgc                                                                      >  2:63765/1‑150 (MQ=255)
                                                                                ggTTATCCAGTTGGCACACCGCCAATATCTCGACGATAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCctgct                                                                     <  1:823668/150‑1 (MQ=255)
                                                                                ggTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCctgct                                                                     <  2:4059644/150‑1 (MQ=255)
                                                                                ggTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCctgct                                                                     >  2:1896161/1‑150 (MQ=255)
                                                                                  ttATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGa                                                                   >  2:4475077/1‑150 (MQ=255)
                                                                                   tATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGAt                                                                  >  2:310407/1‑150 (MQ=255)
                                                                                    aTCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATc                                                                 <  1:1310751/150‑1 (MQ=255)
                                                                                    aTCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATc                                                                 <  2:2488083/150‑1 (MQ=255)
                                                                                    aTCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATc                                                                 <  1:2353028/150‑1 (MQ=255)
                                                                                       cAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTc                                                              <  1:1699799/150‑1 (MQ=255)
                                                                                          ttGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGAc                                                           >  2:658436/1‑150 (MQ=255)
                                                                                          ttGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGAc                                                           >  2:2520800/1‑150 (MQ=255)
                                                                                           tGGCAAACCGCCAATATCTCTACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTCTCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACg                                                          <  2:3070905/150‑1 (MQ=255)
                                                                                            ggCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACgg                                                         >  2:419892/1‑150 (MQ=255)
                                                                                            ggCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACgg                                                         >  2:471518/1‑150 (MQ=255)
                                                                                             gCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGc                                                        <  2:4563099/150‑1 (MQ=255)
                                                                                                  ccGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAAc                                                   >  2:4408912/1‑150 (MQ=255)
                                                                                                    gCCAATCTCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCg                                                 <  1:2073777/150‑1 (MQ=255)
                                                                                                      cAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGAt                                               >  2:3333720/1‑150 (MQ=255)
                                                                                                        atatCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATcc                                             <  1:1081321/150‑1 (MQ=255)
                                                                                                        atatCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATcc                                             >  1:4241096/1‑150 (MQ=255)
                                                                                                        atatCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATcc                                             >  2:1843195/1‑150 (MQ=255)
                                                                                                           tctcGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGca                                          <  2:159763/150‑1 (MQ=255)
                                                                                                               gacgaGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAAc                                      <  1:2260052/150‑1 (MQ=255)
                                                                                                               gacgaGAAAAGCGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAAc                                      >  2:3782097/1‑150 (MQ=255)
                                                                                                                  gataaaaGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTc                                   <  1:1206204/147‑1 (MQ=255)
                                                                                                                  gagaAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTc                                   >  2:526712/1‑150 (MQ=255)
                                                                                                                   agaAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCg                                  >  1:2228695/1‑150 (MQ=255)
                                                                                                                    gaAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCgg                                 >  2:1931396/1‑150 (MQ=255)
                                                                                                                     aaaaGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAAACGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGgc                                >  2:280510/1‑150 (MQ=255)
                                                                                                                      aaaGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGgcg                               <  1:332791/150‑1 (MQ=255)
                                                                                                                        aGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGcgcg                             >  1:498750/1‑150 (MQ=255)
                                                                                                                          cGACGGTGCCGTGCATCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTg                           <  2:3392290/150‑1 (MQ=255)
                                                                                                                           gACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGc                          >  2:3574308/1‑150 (MQ=255)
                                                                                                                           gACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGc                          >  1:3619708/1‑150 (MQ=255)
                                                                                                                                tGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCg                     >  2:70424/1‑150 (MQ=255)
                                                                                                                                   cGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAg                  <  1:2038010/150‑1 (MQ=255)
                                                                                                                                    gTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGt                 >  1:3452382/1‑150 (MQ=255)
                                                                                                                                     tGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTg                >  2:4347039/1‑150 (MQ=255)
                                                                                                                                     tGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTg                <  1:1752510/150‑1 (MQ=255)
                                                                                                                                       cATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCg              >  2:2677035/1‑150 (MQ=255)
                                                                                                                                        aTCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGTTCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCgg             >  2:4204959/1‑150 (MQ=255)
                                                                                                                                        aTCAGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCgg             >  2:416773/1‑150 (MQ=255)
                                                                                                                                          cAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGAc           >  1:1336563/1‑150 (MQ=255)
                                                                                                                                           aGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGAcg          <  1:1000126/150‑1 (MQ=255)
                                                                                                                                           aGGGCATTATAGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGAcg          <  2:1435578/150‑1 (MQ=255)
                                                                                                                                             ggCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGAcgcc        >  1:816822/1‑150 (MQ=255)
                                                                                                                                                 ttATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAg    >  1:3284917/1‑150 (MQ=255)
                                                                                                                                                  tATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAgg   >  1:4518293/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTTCAGGAAGTCTTTATTTTAAAAGGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGC  >  CP009273/4399155‑4399451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑