Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,401,343 A→T 18.7% S75S (TCA→TCT)  yjfK → DUF2491 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,401,3430AT18.7% 698.6 / 147.7 271S75S (TCA→TCT) yjfKDUF2491 family protein
Reads supporting (aligned to +/- strand):  ref base A (121/99);  new base T (22/29);  total (143/128)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 274 total aligned reads displayed.

CTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAAT  >  CP009273/4401194‑4401492
                                                                                                                                                     |                                                                                                                                                     
ctcAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCa                                                                                                                                                       <  2:1429153/150‑1 (MQ=255)
  cAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAgg                                                                                                                                                     <  2:279713/150‑1 (MQ=255)
   aaTAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGc                                                                                                                                                    >  1:88016/1‑150 (MQ=255)
     tAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGa                                                                                                                                                  >  1:4006914/1‑150 (MQ=255)
         ggCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAg                                                                                                                                              >  2:816273/1‑150 (MQ=255)
          gCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGt                                                                                                                                             >  2:2982285/1‑150 (MQ=255)
           cTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGtt                                                                                                                                            >  2:340903/1‑150 (MQ=255)
           cTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGtt                                                                                                                                            >  2:3222971/1‑150 (MQ=255)
            tttACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGttt                                                                                                                                           <  2:851043/150‑1 (MQ=255)
             ttACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTc                                                                                                                                          <  1:2636466/150‑1 (MQ=255)
             ttACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTc                                                                                                                                          >  1:330435/1‑150 (MQ=255)
              tACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCt                                                                                                                                         <  2:1709457/150‑1 (MQ=255)
              tACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCt                                                                                                                                         <  2:642533/150‑1 (MQ=255)
               aCGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTg                                                                                                                                        >  1:3409068/1‑150 (MQ=255)
                cGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGc                                                                                                                                       >  1:1162783/1‑150 (MQ=255)
                 gCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCa                                                                                                                                      <  2:167325/150‑1 (MQ=255)
                     gACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATc                                                                                                                                  >  1:348427/1‑150 (MQ=255)
                      acacGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGACTTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCACATCa                                                                                                                                 >  1:570677/1‑150 (MQ=255)
                      acacGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCa                                                                                                                                 <  2:47722/150‑1 (MQ=255)
                       cacGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCaa                                                                                                                                >  2:2920260/1‑150 (MQ=255)
                         cGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATa                                                                                                                              >  1:4478993/1‑150 (MQ=255)
                          gTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATac                                                                                                                             >  2:2365121/1‑150 (MQ=255)
                          gTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATac                                                                                                                             >  2:609299/1‑150 (MQ=255)
                          gTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATac                                                                                                                             >  1:1017617/1‑150 (MQ=255)
                           ttAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATacc                                                                                                                            <  1:3604002/150‑1 (MQ=255)
                           ttAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATacc                                                                                                                            <  1:4539975/150‑1 (MQ=255)
                            tAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATacca                                                                                                                           >  2:1254523/1‑150 (MQ=255)
                             aGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATaccac                                                                                                                          >  1:851495/1‑150 (MQ=255)
                              gCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATaccacc                                                                                                                         <  2:705686/150‑1 (MQ=255)
                               cgtttcgtttGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACcg                                                                                                                        <  2:2292069/150‑1 (MQ=255)
                               cgtttcgtttGATGGAAGATGAATTGCTGATTGCGCTGCCGGGTTAGGAATTTTCGGTAGCCGCCGTCAGCCACATCGATCTGGGGGGCTGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATACCACcg                                                                                                                        <  2:291514/150‑1 (MQ=255)
                                     gtttGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGgcggcg                                                                                                                  >  1:3264095/1‑150 (MQ=255)
                                       ttGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGaa                                                                                                                >  1:142096/1‑150 (MQ=255)
                                         gCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGa                                                                                                              <  1:4636139/150‑1 (MQ=255)
                                           tGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGata                                                                                                            >  1:4548214/1‑150 (MQ=255)
                                              aaGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATtg                                                                                                         <  1:3831008/150‑1 (MQ=255)
                                               aGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATtga                                                                                                        >  2:4101907/1‑150 (MQ=255)
                                                gATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATtgat                                                                                                       <  1:3682535/150‑1 (MQ=255)
                                                gATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATtgat                                                                                                       <  1:2130301/150‑1 (MQ=255)
                                                 aTGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATtgatg                                                                                                      <  2:2057247/150‑1 (MQ=255)
                                                 aTGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATtgatg                                                                                                      >  1:4160926/1‑150 (MQ=255)
                                                 aTGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATtgatg                                                                                                      >  1:4160764/1‑150 (MQ=255)
                                                   gAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGAc                                                                                                    >  2:1749798/1‑150 (MQ=255)
                                                   gAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGAc                                                                                                    <  2:263826/150‑1 (MQ=255)
                                                    aaTTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACa                                                                                                   >  1:1713299/1‑150 (MQ=255)
                                                     aTTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACAt                                                                                                  >  2:2077271/1‑150 (MQ=255)
                                                        gCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATtga                                                                                               >  2:928825/1‑150 (MQ=255)
                                                         cTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATtgat                                                                                              <  1:4080869/150‑1 (MQ=255)
                                                          tGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCGCCGGCGGCGAAGATATTGATGACATtgatg                                                                                             >  1:2725027/1‑150 (MQ=255)
                                                          tGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATtgatg                                                                                             >  2:4118285/1‑150 (MQ=255)
                                                           gATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATtgatga                                                                                            >  1:2371468/1‑150 (MQ=255)
                                                            aTTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTgatgat                                                                                           <  1:2448223/150‑1 (MQ=255)
                                                              tGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGatat                                                                                         >  1:41463/1‑150 (MQ=255)
                                                                cgcTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCa                                                                                       <  2:4370816/150‑1 (MQ=255)
                                                                   tGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGc                                                                                    <  1:2599245/150‑1 (MQ=255)
                                                                    gCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGct                                                                                   <  1:1991417/150‑1 (MQ=255)
                                                                     ccGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGctc                                                                                  >  1:1759054/1‑150 (MQ=255)
                                                                      cGGGTGAGGAAATTACGGTAGCCGCCGTCAGCCACATCTATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGctct                                                                                 >  1:3232468/1‑150 (MQ=255)
                                                                       gggTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCtt                                                                                >  1:2202457/1‑150 (MQ=255)
                                                                        ggTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCttt                                                                               <  2:2822701/150‑1 (MQ=255)
                                                                            aGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCt                                                                           <  2:1144441/150‑1 (MQ=255)
                                                                            aGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCt                                                                           <  2:4217212/150‑1 (MQ=255)
                                                                              gAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGGAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACCTTGATGATATCAAGCTCTTTGTCTAt                                                                         >  1:121330/1‑150 (MQ=255)
                                                                               aaTTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATg                                                                        >  1:2226370/1‑150 (MQ=255)
                                                                                        tAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCt                                                               <  2:1285542/150‑1 (MQ=255)
                                                                                         aGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTa                                                              <  1:2045742/150‑1 (MQ=255)
                                                                                          gccgccGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTAc                                                             <  1:2329417/150‑1 (MQ=255)
                                                                                           ccgccgTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACg                                                            <  2:408310/150‑1 (MQ=255)
                                                                                             gccgTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGt                                                          <  2:4024097/150‑1 (MQ=255)
                                                                                              ccgTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTa                                                         <  1:70311/150‑1 (MQ=255)
                                                                                              ccgTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTa                                                         <  1:4321842/150‑1 (MQ=255)
                                                                                                gTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATc                                                       <  1:2978725/150‑1 (MQ=255)
                                                                                                gTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATc                                                       <  2:369207/150‑1 (MQ=255)
                                                                                                 tCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCa                                                      >  1:3090028/1‑150 (MQ=255)
                                                                                                   aGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGt                                                    >  1:3203933/1‑150 (MQ=255)
                                                                                                      cacaTCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTaaa                                                 >  1:424654/1‑150 (MQ=255)
                                                                                                       acaTCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAag                                                >  1:542758/1‑150 (MQ=255)
                                                                                                         aTCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAagag                                              >  2:2600682/1‑150 (MQ=255)
                                                                                                                       cggTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGa                                <  2:3550953/150‑1 (MQ=255)
                                                                                                                         gTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTGTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAgg                              >  1:1593812/1‑150 (MQ=255)
                                                                                                                          tAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGc                             >  2:3104923/1‑150 (MQ=255)
                                                                                                                          tAGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGc                             >  2:1953388/1‑150 (MQ=255)
                                                                                                                           aGTCAGATTTTCCGCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCg                            <  2:3826380/150‑1 (MQ=255)
                                                                                                                            gTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGa                           >  2:2575219/1‑150 (MQ=255)
                                                                                                                              cAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATc                         >  2:3928001/1‑150 (MQ=255)
                                                                                                                                  ttttCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACg                     >  2:1479767/1‑150 (MQ=255)
                                                                                                                                    ttCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGcc                   >  1:1426058/1‑150 (MQ=255)
                                                                                                                                    ttCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGcc                   <  1:627471/150‑1 (MQ=255)
                                                                                                                                     tCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCa                  <  1:4405398/150‑1 (MQ=255)
                                                                                                                                     tCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCa                  <  1:2177280/150‑1 (MQ=255)
                                                                                                                                        gCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAg               >  1:2512535/1‑150 (MQ=255)
                                                                                                                                        gCTACTACACTTCTGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAg               >  1:286482/1‑150 (MQ=255)
                                                                                                                                        gCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAg               <  1:2545366/150‑1 (MQ=255)
                                                                                                                                         ctactaCACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGc              <  1:673115/150‑1 (MQ=255)
                                                                                                                                           actacACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGa            <  1:3222971/150‑1 (MQ=255)
                                                                                                                                           actacACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGa            <  1:1926197/150‑1 (MQ=255)
                                                                                                                                            ctacACTTCTGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGAt           <  2:4478934/150‑1 (MQ=255)
                                                                                                                                              acacTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATgg         >  2:453960/1‑150 (MQ=255)
                                                                                                                                               cacTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATggg        >  2:3016769/1‑150 (MQ=255)
                                                                                                                                                acTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGa       <  1:1332397/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACATTGATGATATCAAGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAAT  >  CP009273/4401194‑4401492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑