Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,458,254 T→A 100% V271V (GTT→GTA)  mgtA → magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,458,2540TA97.1% 1086.2 / 14.1 317V271V (GTT→GTA) mgtAmagnesium transporter
Reads supporting (aligned to +/- strand):  ref base T (1/9);  new base A (155/152);  total (156/161)
Fisher's exact test for biased strand distribution p-value = 1.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 317 total aligned reads displayed.

CGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTT  >  CP009273/4458107‑4458400
                                                                                                                                                   |                                                                                                                                                  
cGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACACGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAg                                                                                                                                                  <  2:1870851/150‑1 (MQ=255)
 gCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGt                                                                                                                                                 >  1:1603705/1‑150 (MQ=255)
  cTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACTCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTg                                                                                                                                                >  1:100942/1‑150 (MQ=255)
   tACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGa                                                                                                                                               <  1:79649/150‑1 (MQ=255)
   tACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGa                                                                                                                                               <  1:4451004/150‑1 (MQ=255)
      cACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCa                                                                                                                                            >  2:3803630/1‑150 (MQ=255)
      cACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCa                                                                                                                                            >  2:4556596/1‑150 (MQ=255)
       aCTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAg                                                                                                                                           >  1:3968733/1‑150 (MQ=255)
       aCTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAg                                                                                                                                           <  1:3620435/150‑1 (MQ=255)
          cGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGaa                                                                                                                                        <  2:2871578/150‑1 (MQ=255)
            ccAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAg                                                                                                                                      >  1:3838122/1‑150 (MQ=255)
            ccAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAg                                                                                                                                      <  1:4487285/150‑1 (MQ=255)
            ccAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAg                                                                                                                                      >  2:2294953/1‑150 (MQ=255)
             cAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGc                                                                                                                                     <  1:882400/150‑1 (MQ=255)
             cAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGc                                                                                                                                     >  2:346714/1‑150 (MQ=255)
             cAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGc                                                                                                                                     >  2:346688/1‑150 (MQ=255)
              aGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCg                                                                                                                                    <  1:191304/150‑1 (MQ=255)
              aGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCg                                                                                                                                    <  2:3039715/150‑1 (MQ=255)
               gCCGGAGCACAGCACTCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGa                                                                                                                                   <  1:1389639/150‑1 (MQ=255)
               gCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGa                                                                                                                                   <  1:2332058/150‑1 (MQ=255)
                 cGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGc                                                                                                                                 >  2:2697639/1‑150 (MQ=255)
                  ggAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGcc                                                                                                                                >  2:602389/1‑150 (MQ=255)
                   gAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCg                                                                                                                               >  2:3876684/1‑150 (MQ=255)
                      cacaGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAAt                                                                                                                            <  1:2207803/150‑1 (MQ=255)
                      cacaGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCAGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAAt                                                                                                                            <  1:3143239/150‑1 (MQ=255)
                          gCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCACCTGGCGGGGCGTGTAAGTGAGCAGGACAGCGAGCCGAATGCCt                                                                                                                        >  2:2549607/1‑150 (MQ=255)
                            aaTCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGTCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGACttt                                                                                                                      >  1:3744942/1‑150 (MQ=255)
                             aTCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTc                                                                                                                     <  1:4448193/150‑1 (MQ=255)
                             aTCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTc                                                                                                                     <  1:3243379/150‑1 (MQ=255)
                             aTCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTc                                                                                                                     >  2:402606/1‑150 (MQ=255)
                               ccGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAg                                                                                                                   >  2:2664009/1‑150 (MQ=255)
                                 gCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCa                                                                                                                 <  2:2812965/150‑1 (MQ=255)
                                   tGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAg                                                                                                               >  1:95048/1‑150 (MQ=255)
                                    ggAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAgg                                                                                                              <  2:1424359/150‑1 (MQ=255)
                                      aGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGa                                                                                                            >  2:2594938/1‑150 (MQ=255)
                                       gTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAt                                                                                                           >  2:3588695/1‑150 (MQ=255)
                                        tGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATc                                                                                                          >  1:3576310/1‑150 (MQ=255)
                                         gCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCa                                                                                                         <  1:1411902/150‑1 (MQ=255)
                                          cGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAg                                                                                                        >  2:4073793/1‑150 (MQ=255)
                                             cacGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCcg                                                                                                     <  2:1828936/150‑1 (MQ=255)
                                                gCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGt                                                                                                  >  1:1228198/1‑150 (MQ=255)
                                                gCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGt                                                                                                  <  1:1576459/150‑1 (MQ=255)
                                                 cTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTc                                                                                                 <  1:4208271/150‑1 (MQ=255)
                                                     gtTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTa                                                                                             <  1:4337147/150‑1 (MQ=255)
                                                      ttttATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAt                                                                                            >  1:2734932/1‑150 (MQ=255)
                                                      ttttATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAt                                                                                            <  1:1694090/150‑1 (MQ=255)
                                                      ttttATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAt                                                                                            <  1:1306713/150‑1 (MQ=255)
                                                      ttttATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAt                                                                                            <  1:3626802/150‑1 (MQ=255)
                                                        ttATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAtgc                                                                                          >  2:1585001/1‑150 (MQ=255)
                                                         tATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAtgct                                                                                         >  1:3756253/1‑150 (MQ=255)
                                                         tATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAtgct                                                                                         <  1:2838156/150‑1 (MQ=255)
                                                          aTGGGCACCACCGTGGTGAGCGGCACGTCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGATCAGGAAAGCGAGCCGCATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAtgctg                                                                                        <  2:813043/150‑1 (MQ=255)
                                                          aTGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAtgctg                                                                                        >  1:2772710/1‑150 (MQ=255)
                                                            gggCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATgctgct                                                                                      <  1:17523/150‑1 (MQ=255)
                                                              gCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGa                                                                                    <  2:2923339/150‑1 (MQ=255)
                                                                accaccGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTATCAACAAGGGATCAGCCGCGACAGTATGCTGCTGAtt                                                                                  >  1:4593585/1‑150 (MQ=255)
                                                                      gtggtgAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCttt                                                                            >  2:3216776/1‑150 (MQ=255)
                                                                      gtggtgAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCttt                                                                            <  2:2422532/150‑1 (MQ=255)
                                                                       tggtgAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTa                                                                           <  1:678159/150‑1 (MQ=255)
                                                                           gAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCt                                                                       <  2:65102/150‑1 (MQ=255)
                                                                                gcacggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGACTGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGa                                                                  <  1:2707236/150‑1 (MQ=255)
                                                                                 cacggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGAt                                                                 <  2:1815790/150‑1 (MQ=255)
                                                                                   cggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATgg                                                               >  2:4365550/1‑150 (MQ=255)
                                                                                    ggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGc                                                              <  1:4077322/150‑1 (MQ=255)
                                                                                        caAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCg                                                          >  2:2241116/1‑150 (MQ=255)
                                                                                        caAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCg                                                          <  2:247006/150‑1 (MQ=255)
                                                                                          aGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCggt                                                        <  1:4390841/150‑1 (MQ=255)
                                                                                            cAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCggtgg                                                      <  2:3690477/150‑1 (MQ=255)
                                                                                             aaTGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCggtggt                                                     >  1:3816061/1‑150 (MQ=255)
                                                                                             aaTGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCggtggt                                                     >  1:950793/1‑150 (MQ=255)
                                                                                               tGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGc                                                   >  2:938398/1‑150 (MQ=255)
                                                                                                    aTTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTa                                              >  1:2114566/1‑150 (MQ=255)
                                                                                                    aTTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTa                                              >  1:906253/1‑150 (MQ=255)
                                                                                                      tGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAAt                                            >  2:1393636/1‑150 (MQ=255)
                                                                                                          aCAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAAt                                        <  2:2401947/150‑1 (MQ=255)
                                                                                                          aCAGGTGCCAAGACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAAt                                        <  2:283091/150‑1 (MQ=255)
                                                                                                           cAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATg                                       >  1:1266756/1‑150 (MQ=255)
                                                                                                            aGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGTAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATgg                                      >  2:4592534/1‑150 (MQ=255)
                                                                                                            aGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGTAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATgg                                      >  2:4086025/1‑150 (MQ=255)
                                                                                                              gTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGtt                                    <  2:4275653/150‑1 (MQ=255)
                                                                                                                gCCAATACCTGGTTTGGTCAACTGGCGTGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTac                                  <  2:4443672/150‑1 (MQ=255)
                                                                                                                 ccAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTaca                                 >  2:4497873/1‑150 (MQ=255)
                                                                                                                  cAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTacac                                <  2:4075953/150‑1 (MQ=255)
                                                                                                                   aaTACCTGGTTTGGTCAACTTGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACAcc                               <  1:967081/150‑1 (MQ=255)
                                                                                                                   aaTACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACAcc                               <  1:1133701/150‑1 (MQ=255)
                                                                                                                    aTACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCa                              <  1:2928278/150‑1 (MQ=255)
                                                                                                                    aTACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCa                              >  2:1729186/1‑150 (MQ=255)
                                                                                                                           gTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGa                       <  2:1187724/150‑1 (MQ=255)
                                                                                                                            tttGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGAc                      >  1:2216586/1‑150 (MQ=255)
                                                                                                                            tttGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGAc                      >  2:3341270/1‑150 (MQ=255)
                                                                                                                              tGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACtg                    >  1:607707/1‑150 (MQ=255)
                                                                                                                               ggTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACtgg                   >  1:2000891/1‑150 (MQ=255)
                                                                                                                                 tCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACtggtg                 >  1:3409866/1‑150 (MQ=255)
                                                                                                                                   aaCTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTggg               >  2:3109675/1‑150 (MQ=255)
                                                                                                                                    aCTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGa              <  1:125174/150‑1 (MQ=255)
                                                                                                                                       ggCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAgc           <  1:3433695/150‑1 (MQ=255)
                                                                                                                                       ggCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAgc           <  1:4630134/150‑1 (MQ=255)
                                                                                                                                        gCGGGGAGTGTAAGTGAGAAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGATGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATTGTTACACCAAAGGCGACTTGTGGGAAgcg          <  1:2501702/150‑1 (MQ=255)
                                                                                                                                          ggggCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAgcggc        >  2:4220622/1‑150 (MQ=255)
                                                                                                                                                tgtAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGtt  >  2:2016147/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTT  >  CP009273/4458107‑4458400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑