Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,465,836 C→T 12.2% L195L (CTA→TTA)  yjgL → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,465,8360CT12.2% 862.3 / 79.4 279L195L (CTA→TTA) yjgLuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (126/119);  new base T (20/14);  total (146/133)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

GTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAG  >  CP009273/4465687‑4465985
                                                                                                                                                     |                                                                                                                                                     
gTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATc                                                                                                                                                       >  1:2430758/1‑150 (MQ=255)
  tCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTa                                                                                                                                                     <  2:1228513/150‑1 (MQ=255)
    gACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACa                                                                                                                                                   <  2:2144984/150‑1 (MQ=255)
     aCTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAg                                                                                                                                                  <  2:4328909/150‑1 (MQ=255)
       tAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGaa                                                                                                                                                <  2:1069045/150‑1 (MQ=255)
       tAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTACATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGaa                                                                                                                                                <  2:138928/150‑1 (MQ=255)
        aaCCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGaaa                                                                                                                                               >  1:2262728/1‑150 (MQ=255)
         aCCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAAt                                                                                                                                              <  2:1950174/150‑1 (MQ=255)
         aCCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAAt                                                                                                                                              <  1:1554007/150‑1 (MQ=255)
              tATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAaat                                                                                                                                         >  1:1504002/1‑150 (MQ=255)
               aTGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAaata                                                                                                                                        <  1:2547173/150‑1 (MQ=255)
               aTGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAaata                                                                                                                                        <  2:3009185/150‑1 (MQ=255)
               aTGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGAGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAAGAATAATCAGGAGTTAAATAATCTACAGAAATTAaata                                                                                                                                        <  2:694759/150‑1 (MQ=255)
                  aaGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATc                                                                                                                                     >  2:3088277/1‑150 (MQ=255)
                   aGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCt                                                                                                                                    >  2:3706308/1‑150 (MQ=255)
                   aGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCt                                                                                                                                    <  1:1780211/150‑1 (MQ=255)
                     ggAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTAc                                                                                                                                  <  2:1768506/150‑1 (MQ=255)
                      gAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACa                                                                                                                                 >  2:2937374/1‑150 (MQ=255)
                       aggTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAg                                                                                                                                <  2:3154780/148‑1 (MQ=255)
                       aaGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAg                                                                                                                                >  1:3731250/1‑150 (MQ=255)
                          tCCTTTGAAAGAAAAAATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAg                                                                                                                             >  2:148765/1‑150 (MQ=255)
                               tGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTaaa                                                                                                                        <  2:2195630/150‑1 (MQ=255)
                               tGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTaaa                                                                                                                        >  2:3112169/1‑150 (MQ=255)
                                gaaagaaaAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAaat                                                                                                                       <  1:2904750/150‑1 (MQ=255)
                                gaaagaaaAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAaat                                                                                                                       <  2:4345071/150‑1 (MQ=255)
                                   agaaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAaataat                                                                                                                    >  1:952859/1‑150 (MQ=255)
                                   agaaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTAAAGAAATTAAATAATCTACAGAAGTTAaataat                                                                                                                    >  2:3985470/1‑150 (MQ=255)
                                     aaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCt                                                                                                                  <  1:3599005/150‑1 (MQ=255)
                                        agaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACt                                                                                                               >  2:2970483/1‑150 (MQ=255)
                                          aTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGa                                                                                                             <  1:2854987/150‑1 (MQ=255)
                                             agagTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGt                                                                                                          >  1:2068827/1‑150 (MQ=255)
                                                  tCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAaat                                                                                                     <  2:2577948/150‑1 (MQ=255)
                                                   cATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAaata                                                                                                    >  1:2984874/1‑150 (MQ=255)
                                                     ttACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAaataat                                                                                                  <  2:896001/150‑1 (MQ=255)
                                                      tACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAataata                                                                                                 >  2:1446775/1‑150 (MQ=255)
                                                       acacCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAatat                                                                                                >  2:3638968/1‑150 (MQ=255)
                                                       acacCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAatat                                                                                                <  2:222574/150‑1 (MQ=255)
                                                       acacCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAAGAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAatat                                                                                                <  2:222325/150‑1 (MQ=255)
                                                         acCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATAc                                                                                              <  1:3261086/150‑1 (MQ=255)
                                                         acCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATAc                                                                                              >  1:3892467/1‑150 (MQ=255)
                                                            tGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAgg                                                                                           >  1:3365664/1‑150 (MQ=255)
                                                            tGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGATAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACATAAATTAAATAATCTACAGAAGTTAAATAATATAATGAAGTAAAATAATATACAgg                                                                                           >  1:748966/1‑150 (MQ=255)
                                                             gATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAggg                                                                                          >  2:4606059/1‑150 (MQ=255)
                                                             gATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAggg                                                                                          >  2:1903844/1‑150 (MQ=255)
                                                               ttACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGt                                                                                        <  2:4396273/150‑1 (MQ=255)
                                                                 aCTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTa                                                                                      <  2:1102997/150‑1 (MQ=255)
                                                                  cTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTaa                                                                                     <  1:2166084/150‑1 (MQ=255)
                                                                  cTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTaa                                                                                     <  1:2789071/150‑1 (MQ=255)
                                                                  cTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTaa                                                                                     <  1:332357/150‑1 (MQ=255)
                                                                   tAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTaaa                                                                                    >  2:3367393/1‑150 (MQ=255)
                                                                    aaCAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAaat                                                                                   <  1:4115543/150‑1 (MQ=255)
                                                                       aaGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAaataat                                                                                <  2:779282/150‑1 (MQ=255)
                                                                        aGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATc                                                                               >  1:1568252/1‑150 (MQ=255)
                                                                          gTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCt                                                                             >  2:544499/1‑150 (MQ=255)
                                                                            tCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCa                                                                           <  1:4500082/150‑1 (MQ=255)
                                                                            tCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCa                                                                           <  2:1844103/150‑1 (MQ=255)
                                                                                 cGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGt                                                                      <  2:3764646/150‑1 (MQ=255)
                                                                                 cGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGt                                                                      <  1:4601979/150‑1 (MQ=255)
                                                                                  gtgtTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGtt                                                                     >  2:108216/1‑150 (MQ=255)
                                                                                       aCCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAaata                                                                >  2:68616/1‑150 (MQ=255)
                                                                                       aCCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAaata                                                                <  2:2982964/150‑1 (MQ=255)
                                                                                        ccAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACNGGGGTTAAATAATCCTCAGGAGTTAaataa                                                               <  1:4284009/150‑1 (MQ=255)
                                                                                        ccAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAaataa                                                               >  1:3064248/1‑150 (MQ=255)
                                                                                            aGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCg                                                           >  2:1454040/1‑150 (MQ=255)
                                                                                              tGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCa                                                         <  1:1777308/150‑1 (MQ=255)
                                                                                              tGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCa                                                         >  1:4426377/1‑150 (MQ=255)
                                                                                               gAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAg                                                        >  1:2575589/1‑150 (MQ=255)
                                                                                               gAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAg                                                        <  1:3135584/150‑1 (MQ=255)
                                                                                                   cTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAAtt                                                    <  1:1810113/150‑1 (MQ=255)
                                                                                                   cTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAAtt                                                    >  2:102607/1‑150 (MQ=255)
                                                                                                    tAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAAttt                                                   <  2:550115/150‑1 (MQ=255)
                                                                                                    tAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAAttt                                                   >  1:2695154/1‑150 (MQ=255)
                                                                                                       cAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTaaa                                                <  2:3876273/150‑1 (MQ=255)
                                                                                                       cAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTaaa                                                >  1:2073451/1‑150 (MQ=255)
                                                                                                       cAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTaaa                                                >  1:850644/1‑150 (MQ=255)
                                                                                                         aGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATg                                              <  1:4230694/150‑1 (MQ=255)
                                                                                                          gAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGa                                             >  2:3907161/1‑150 (MQ=255)
                                                                                                           aaaaTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGAt                                            >  2:1987478/1‑150 (MQ=255)
                                                                                                            aaaTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGAtt                                           >  2:4178768/1‑150 (MQ=255)
                                                                                                            aaaTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGAtt                                           >  2:3473793/1‑150 (MQ=255)
                                                                                                              aTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATtct                                         <  1:1364771/150‑1 (MQ=255)
                                                                                                              aTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATtct                                         <  2:164010/150‑1 (MQ=255)
                                                                                                                    cgTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAg                                   >  1:2205542/1‑150 (MQ=255)
                                                                                                                       cTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTa                                <  1:3987049/150‑1 (MQ=255)
                                                                                                                         aTTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTaaa                              >  1:1071549/1‑150 (MQ=255)
                                                                                                                           tAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATa                            <  1:4014415/150‑1 (MQ=255)
                                                                                                                              ataataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACt                         >  1:3298264/1‑150 (MQ=255)
                                                                                                                                 ataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGc                      >  2:4167573/1‑150 (MQ=255)
                                                                                                                                   aatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAg                    <  2:3265939/150‑1 (MQ=255)
                                                                                                                                    atCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAgg                   >  2:2701960/1‑150 (MQ=255)
                                                                                                                                       aGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAAt                >  1:552687/1‑150 (MQ=255)
                                                                                                                                          aGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTaa             >  2:2292037/1‑150 (MQ=255)
                                                                                                                                           gTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTaaa            <  1:3008364/150‑1 (MQ=255)
                                                                                                                                            ttAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAAt           <  1:1859354/150‑1 (MQ=255)
                                                                                                                                             tAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATa          >  2:3331216/1‑150 (MQ=255)
                                                                                                                                              aaaTAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGACTTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAg         >  2:1542827/1‑150 (MQ=255)
                                                                                                                                               aataatCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGt        <  2:3723902/150‑1 (MQ=255)
                                                                                                                                                 taatCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTcc      >  2:4127889/1‑150 (MQ=255)
                                                                                                                                                     cTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAg  <  1:3723955/150‑1 (MQ=255)
                                                                                                                                                     cTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAg  <  1:606193/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAG  >  CP009273/4465687‑4465985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑