BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,465,936	T→A	7.5%	L228* (TTA→TAA)	yjgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,465,936	0	T	A	7.5%	830.3 / 45.1	252	L228* (TTA→TAA)	yjgL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (138/95); new base A (9/10); total (147/105)
Fisher's exact test for biased strand distribution p-value = 3.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 260 total aligned reads displayed.

TAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGTTTATTAA  >  CP009273/4465787‑4466085

tAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAAttt                                                                                                                                                       >  1:2695154/1‑150 (MQ=255)

tAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAAttt                                                                                                                                                       >  1:3325917/1‑150 (MQ=255)

gaaCAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAAttt                                                                                                                                                       <  2:550111/149‑1 (MQ=255)

 aaCAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTa                                                                                                                                                      <  1:2260194/150‑1 (MQ=255)

   cAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTaaa                                                                                                                                                    <  2:3876273/150‑1 (MQ=255)

   cAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTaaa                                                                                                                                                    >  1:850644/1‑150 (MQ=255)

    aaGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAAt                                                                                                                                                   >  2:3570675/1‑150 (MQ=255)

      gAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGa                                                                                                                                                 >  2:1943885/1‑150 (MQ=255)

      gAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGa                                                                                                                                                 >  2:3907161/1‑150 (MQ=255)

       aaaaTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGAt                                                                                                                                                >  2:2205523/1‑150 (MQ=255)

        aaaTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGAtt                                                                                                                                               >  2:4178768/1‑150 (MQ=255)

         aaTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATtc                                                                                                                                              >  1:503285/1‑150 (MQ=255)

          aTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATtct                                                                                                                                             >  2:1480732/1‑150 (MQ=255)

           tAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATtctc                                                                                                                                            >  1:2069402/1‑150 (MQ=255)

                cgTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAg                                                                                                                                       >  1:2569252/1‑150 (MQ=255)

                  tCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGtt                                                                                                                                     >  1:430712/1‑150 (MQ=255)

                   cTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTa                                                                                                                                    <  1:3987046/150‑1 (MQ=255)

                     aTTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTaaa                                                                                                                                  >  1:1071549/1‑150 (MQ=255)

                     aTTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTaaa                                                                                                                                  >  1:82673/1‑150 (MQ=255)

                      ttAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAAt                                                                                                                                 <  2:3055317/150‑1 (MQ=255)

                       tAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATa                                                                                                                                <  1:4014415/150‑1 (MQ=255)

                        aaaTAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATaa                                                                                                                               >  1:2201415/1‑150 (MQ=255)

                        aaaTAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATaa                                                                                                                               >  2:4053894/1‑150 (MQ=255)

                            aataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCg                                                                                                                           >  1:4636564/1‑150 (MQ=255)

                               aatCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAg                                                                                                                        >  2:3463073/1‑150 (MQ=255)

                                atCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAgg                                                                                                                       <  2:4254777/150‑1 (MQ=255)

                                   aGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAAt                                                                                                                    >  1:552687/1‑150 (MQ=255)

                                    ggAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAAtt                                                                                                                   <  1:2317878/150‑1 (MQ=255)

                                    ggAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAAtt                                                                                                                   >  2:2706704/1‑150 (MQ=255)

                                       gTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTaaa                                                                                                                <  2:1352826/150‑1 (MQ=255)

                                         tAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATa                                                                                                              >  2:3145964/1‑150 (MQ=255)

                                         tAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATa                                                                                                              >  2:1977522/1‑150 (MQ=255)

                                          aaaTAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGACTTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAg                                                                                                             >  2:1542827/1‑150 (MQ=255)

                                           aataatCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGt                                                                                                            <  2:3723902/150‑1 (MQ=255)

                                             taatCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTcc                                                                                                          >  2:1048812/1‑150 (MQ=255)

                                              aatCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCca                                                                                                         >  1:4065170/1‑150 (MQ=255)

                                                 cTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAg                                                                                                      <  1:606193/150‑1 (MQ=255)

                                                       aaaTTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTa                                                                                                >  1:7708/1‑150 (MQ=255)

                                                        aaTTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTaa                                                                                               <  2:2871829/150‑1 (MQ=255)

                                                        aaTTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTaa                                                                                               <  2:215506/150‑1 (MQ=255)

                                                           tAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATg                                                                                            <  2:66444/150‑1 (MQ=255)

                                                           tAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATg                                                                                            >  1:4256856/1‑150 (MQ=255)

                                                           tAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATg                                                                                            >  2:3063350/1‑150 (MQ=255)

                                                             aataatCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGAt                                                                                          >  1:3023890/1‑150 (MQ=255)

                                                              ataatCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATc                                                                                         >  2:3212340/1‑150 (MQ=255)

                                                              ataatCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATc                                                                                         <  2:2040105/150‑1 (MQ=255)

                                                               taatCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATcc                                                                                        >  2:2677010/1‑150 (MQ=255)

                                                                   cTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAg                                                                                    <  2:2430758/150‑1 (MQ=255)

                                                                      cAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAg                                                                                 >  1:4624247/1‑150 (MQ=255)

                                                                        gAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGtt                                                                               <  1:3088277/150‑1 (MQ=255)

                                                                           gTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTaaa                                                                            >  1:1356342/1‑150 (MQ=255)

                                                                                 taatCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATtc                                                                      <  1:3112169/150‑1 (MQ=255)

                                                                                  aatCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATtct                                                                     >  2:3320318/1‑150 (MQ=255)

                                                                                   atCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATtctc                                                                    >  1:3491427/1‑150 (MQ=255)

                                                                                      tACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAgg                                                                 <  1:93194/150‑1 (MQ=255)

                                                                                          gAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACtt                                                             <  2:676625/150‑1 (MQ=255)

                                                                                              ttAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAAt                                                         <  1:3618663/150‑1 (MQ=255)

                                                                                                aaaTAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATaa                                                       >  1:1214214/1‑150 (MQ=255)

                                                                                                 aataatATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAAc                                                      >  1:1340088/1‑150 (MQ=255)

                                                                                                 aataatATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAAc                                                      >  2:1788500/1‑150 (MQ=255)

                                                                                                   taataTACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAActc                                                    >  2:4413114/1‑150 (MQ=255)

                                                                                                    aataTACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCTGGACTTAATTAActct                                                   >  2:3345990/1‑150 (MQ=255)

                                                                                                     atatACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTa                                                  >  2:1197548/1‑150 (MQ=255)

                                                                                                     atatACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTa                                                  <  2:3771563/150‑1 (MQ=255)

                                                                                                     atatACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTa                                                  >  1:299716/1‑150 (MQ=255)

                                                                                                      tataCAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTaa                                                 >  1:1635869/1‑150 (MQ=255)

                                                                                                          cAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATAATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTg                                             >  1:3216342/1‑150 (MQ=255)

                                                                                                             gggTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGt                                          >  2:1136642/1‑150 (MQ=255)

                                                                                                                ttAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGt                                       >  1:405578/1‑150 (MQ=255)

                                                                                                                  aaaTAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTCAGGTGAGTTGTAc                                     >  1:765808/1‑150 (MQ=255)

                                                                                                                  aaaTAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTAc                                     >  1:3395137/1‑150 (MQ=255)

                                                                                                                  aaaTAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTAc                                     >  1:3299509/1‑150 (MQ=255)

                                                                                                                  aaaTAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTAc                                     >  1:4492622/1‑150 (MQ=255)

                                                                                                                   aataatCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACa                                    <  2:2070768/150‑1 (MQ=255)

                                                                                                                    ataatCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAg                                   >  2:2282356/1‑150 (MQ=255)

                                                                                                                     taatCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGt                                  <  1:1835864/150‑1 (MQ=255)

                                                                                                                     taatCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGt                                  >  1:3686738/1‑150 (MQ=255)

                                                                                                                         ccTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCa                              >  2:3252193/1‑150 (MQ=255)

                                                                                                                           tCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGt                            >  2:4387082/1‑150 (MQ=255)

                                                                                                                             aGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTg                          <  1:3638968/150‑1 (MQ=255)

                                                                                                                             aGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTg                          <  1:1724861/150‑1 (MQ=255)

                                                                                                                              ggAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGa                         >  2:4605647/1‑150 (MQ=255)

                                                                                                                                 gTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTc                      <  1:3706308/150‑1 (MQ=255)

                                                                                                                                  ttAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCt                     >  1:3873669/1‑150 (MQ=255)

                                                                                                                                       taatCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGAt                >  1:3658301/1‑150 (MQ=255)

                                                                                                                                        aatCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGAtt               >  2:3002243/1‑150 (MQ=255)

                                                                                                                                         atCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTa              >  1:2391946/1‑150 (MQ=255)

                                                                                                                                         atCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTa              <  1:221191/150‑1 (MQ=255)

                                                                                                                                         atCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTa              >  2:2833598/1‑150 (MQ=255)

                                                                                                                                          tCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTAc             >  1:732935/1‑150 (MQ=255)

                                                                                                                                          tCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTAc             <  1:2937374/150‑1 (MQ=255)

                                                                                                                                           ccGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACg            >  1:1406406/1‑150 (MQ=255)

                                                                                                                                            cGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACgg           >  2:1027284/1‑150 (MQ=255)

                                                                                                                                              cAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGt         <  1:4333436/150‑1 (MQ=255)

                                                                                                                                                gAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGttt       >  2:1731307/1‑150 (MQ=255)

                                                                                                                                                 aaTTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGTtta      <  2:564569/150‑1 (MQ=255)

                                                                                                                                                  aTTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGTttat     >  2:866040/1‑150 (MQ=255)

                                                                                                                                                  aTTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGTttat     <  2:3298230/150‑1 (MQ=255)

                                                                                                                                                    ttAAATGATTCTGAGGAGTTTTATTAATGGCATGATTTAAATTTTCAACAGGTGTTAAATGATGAGCAGGAGTTAAATAATTCTAATGTCTTAAATAACTCTTAGGTGAGGTGTAAAGTTTCATTTGTTTATACGATTACGGGTttatta   <  1:1857519/150‑1 (MQ=255)

                                                                                                                                                    ttAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGTttatta   <  1:4185050/150‑1 (MQ=255)

TAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGTTTATTAA  >  CP009273/4465787‑4466085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`