Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,470,873 G→T 91.8% R928S (CGT→AGT)  valS ← valyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,470,8730GT91.8% 739.0 / 57.5 256R928S (CGT→AGT) valSvalyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (7/14);  new base T (122/113);  total (129/127)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 261 total aligned reads displayed.

TCTTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGGCCAGCCATC  >  CP009273/4470726‑4471021
                                                                                                                                                   |                                                                                                                                                    
tcttTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCActct                                                                                                                                                    >  1:1609712/1‑150 (MQ=255)
tcttTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGct                                                                                                                                                    <  1:161816/150‑1 (MQ=255)
    tACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCttt                                                                                                                                                <  1:4559005/150‑1 (MQ=255)
    tACAATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCttt                                                                                                                                                <  1:4432493/150‑1 (MQ=255)
     aCCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTc                                                                                                                                               <  2:4347529/150‑1 (MQ=255)
      ccATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTcg                                                                                                                                              >  1:3639423/1‑150 (MQ=255)
       cATTTCTGTAAGAGATAAAATAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTcgc                                                                                                                                             >  2:1123673/1‑150 (MQ=255)
       cATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCTCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTcgc                                                                                                                                             >  2:4157266/1‑150 (MQ=255)
        aTTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTcgcg                                                                                                                                            <  2:3052581/150‑1 (MQ=255)
          ttCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGAt                                                                                                                                          >  1:218429/1‑150 (MQ=255)
            cTGTAAGAGATAAAAATGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGa                                                                                                                                        >  1:1378166/1‑150 (MQ=255)
            cTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGa                                                                                                                                        >  1:3468918/1‑150 (MQ=255)
               tAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCg                                                                                                                                     >  2:2663281/1‑150 (MQ=255)
                   agaTAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTc                                                                                                                                 >  2:2402710/1‑150 (MQ=255)
                    gaTAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTcc                                                                                                                                <  1:1852967/150‑1 (MQ=255)
                     aTAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCg                                                                                                                               >  1:2345941/1‑150 (MQ=255)
                           aaGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGGGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTgcgc                                                                                                                         >  2:4275565/1‑150 (MQ=255)
                                cGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCg                                                                                                                    >  2:2119537/1‑150 (MQ=255)
                                    gCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACaa                                                                                                                <  1:805886/150‑1 (MQ=255)
                                     cATGCTCCGGCCTTCGTTTTCATCTCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACaaa                                                                                                               <  1:4070652/150‑1 (MQ=255)
                                      aTGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAg                                                                                                              >  2:2292595/1‑150 (MQ=255)
                                       tGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGc                                                                                                             >  2:4458771/1‑150 (MQ=255)
                                       tGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGc                                                                                                             >  2:1637548/1‑150 (MQ=255)
                                          tCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCtt                                                                                                          <  1:2672035/150‑1 (MQ=255)
                                          tCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCtt                                                                                                          >  1:3010515/1‑150 (MQ=255)
                                          tCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCtt                                                                                                          <  1:253871/150‑1 (MQ=255)
                                          tCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCtt                                                                                                          >  1:1509354/1‑150 (MQ=255)
                                           ccGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttc                                                                                                         >  1:1300107/1‑150 (MQ=255)
                                            cGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcg                                                                                                        >  1:426925/1‑150 (MQ=255)
                                             ggCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcgt                                                                                                       >  2:3591980/1‑150 (MQ=255)
                                               ccTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcgttc                                                                                                     >  1:2754939/1‑150 (MQ=255)
                                                 ttCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGc                                                                                                   >  2:557040/1‑150 (MQ=255)
                                                    gTTTTCATCACTGTGTTTTGATTACAGCGCAGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAg                                                                                                >  1:2378655/1‑150 (MQ=255)
                                                     ttttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGt                                                                                               <  2:2989672/150‑1 (MQ=255)
                                                      tttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGtt                                                                                              >  2:2518578/1‑150 (MQ=255)
                                                      tttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGtt                                                                                              >  2:2760401/1‑150 (MQ=255)
                                                         catcaCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGATTGt                                                                                           >  2:2799905/1‑150 (MQ=255)
                                                          atcaCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGtt                                                                                          <  1:3906192/150‑1 (MQ=255)
                                                          atcaCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGtt                                                                                          <  1:921512/150‑1 (MQ=255)
                                                           tcaCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTtc                                                                                         >  2:3230058/1‑150 (MQ=255)
                                                                  gtTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATAc                                                                                  <  2:1975628/150‑1 (MQ=255)
                                                                  gtTTTGATTACAGCGCGGAGATAACAGCATGCTGTTCAATAAGTTTCGCTTTCGATTCCTCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCATTAGTTCGCCAGTTTTTTCTCGATAc                                                                                  >  2:240619/1‑150 (MQ=255)
                                                                   ttttGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACg                                                                                 <  1:2915740/150‑1 (MQ=255)
                                                                      tGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCt                                                                              <  1:1274991/150‑1 (MQ=255)
                                                                       gATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTg                                                                             <  1:2429591/150‑1 (MQ=255)
                                                                       gATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTg                                                                             >  2:3491280/1‑150 (MQ=255)
                                                                        aTTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGa                                                                            >  2:410648/1‑150 (MQ=255)
                                                                        aTTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGa                                                                            >  1:3130735/1‑150 (MQ=255)
                                                                           aCAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGAttt                                                                         <  1:3541484/150‑1 (MQ=255)
                                                                               cgcgGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCAcc                                                                     >  1:558070/1‑150 (MQ=255)
                                                                                 cggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCtt                                                                   <  2:1841307/150‑1 (MQ=255)
                                                                                  ggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTc                                                                  >  2:4039454/1‑150 (MQ=255)
                                                                                  ggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTc                                                                  <  1:1994290/150‑1 (MQ=255)
                                                                                   gcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCa                                                                 >  2:215088/1‑150 (MQ=255)
                                                                                     gATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAATGCCTTCGTTCGCCAGTTTGTTCTCGATACGGATGATTTCACCTTCAAt                                                               <  1:2477120/150‑1 (MQ=255)
                                                                                      aTAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATc                                                              <  1:1530507/150‑1 (MQ=255)
                                                                                       tAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCt                                                             <  2:4584567/150‑1 (MQ=255)
                                                                                       tAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCGGATTTCACCTTCAATCt                                                             >  1:4523217/1‑150 (MQ=255)
                                                                                           aGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGc                                                         <  2:671899/150‑1 (MQ=255)
                                                                                             ccTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCa                                                       >  2:3318143/1‑150 (MQ=255)
                                                                                               tgctgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCAct                                                     >  2:3955108/1‑150 (MQ=255)
                                                                                                  tgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCActtct                                                  <  2:3968362/150‑1 (MQ=255)
                                                                                                     tCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTc                                               <  2:4318077/150‑1 (MQ=255)
                                                                                                     tCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTc                                               <  1:2084183/150‑1 (MQ=255)
                                                                                                      cAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCg                                              <  1:446781/150‑1 (MQ=255)
                                                                                                       aaTCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGTGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGc                                             >  2:4034636/1‑150 (MQ=255)
                                                                                                       aaTCAGTTTCGCTATCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGAGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTATGTTCTCGATACTGATGATTTCACCTTCAATCTTCGCCACTTCTTTCGc                                             >  2:309516/1‑150 (MQ=255)
                                                                                                          cAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAg                                          <  2:3135645/150‑1 (MQ=255)
                                                                                                          cAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAg                                          <  2:269451/150‑1 (MQ=255)
                                                                                                           aGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGa                                         <  2:1515245/150‑1 (MQ=255)
                                                                                                             tttcgctttcgcTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGAcg                                       >  1:350173/1‑150 (MQ=255)
                                                                                                               tcgctttcgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGAcgcg                                     >  2:1094854/1‑150 (MQ=255)
                                                                                                               tcgctttcgcgtCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAATGCCTTCGGTCGCCAGTTTGTTCTCGATACGGCTGTTTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGAcgcg                                     <  1:1071907/150‑1 (MQ=255)
                                                                                                                 gctttcgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGcc                                   >  1:103536/1‑150 (MQ=255)
                                                                                                                   tttcgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAg                                 >  2:382645/1‑150 (MQ=255)
                                                                                                                      cgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTc                              <  2:3308339/150‑1 (MQ=255)
                                                                                                                      cgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTACAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTc                              <  2:297711/150‑1 (MQ=255)
                                                                                                                       gcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCa                             <  2:1927629/150‑1 (MQ=255)
                                                                                                                       gcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCa                             <  1:4421883/150‑1 (MQ=255)
                                                                                                                        cttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAt                            >  1:2299825/1‑150 (MQ=255)
                                                                                                                         ttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtc                           >  2:1720051/1‑150 (MQ=255)
                                                                                                                         ttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtc                           >  2:117166/1‑150 (MQ=255)
                                                                                                                          tCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtct                          <  1:3112782/150‑1 (MQ=255)
                                                                                                                               aTAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCttt                     <  1:746092/150‑1 (MQ=255)
                                                                                                                               aTAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCttt                     >  1:414579/1‑150 (MQ=255)
                                                                                                                                tAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttg                    >  2:552030/1‑150 (MQ=255)
                                                                                                                                  gCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttgtt                  >  2:4509128/1‑150 (MQ=255)
                                                                                                                                  gCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttgtt                  <  1:1761467/150‑1 (MQ=255)
                                                                                                                                  gCCTTCCAGCTTCTCACTCTCTTTCGCGAGGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttgtt                  <  1:505654/150‑1 (MQ=255)
                                                                                                                                     ttCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtgat               >  2:3319842/1‑150 (MQ=255)
                                                                                                                                     ttCCAGCTTCTCACTCTCTTTCGCGAGGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtgat               <  2:2622938/150‑1 (MQ=255)
                                                                                                                                      tCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtgatg              >  2:2220846/1‑150 (MQ=255)
                                                                                                                                      tCCAGCTTCTCACTCTCTTTCGCGAAGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTATGTTCTCGAAACGCCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtgatg              >  1:3146505/1‑150 (MQ=255)
                                                                                                                                       ccAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCCTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtgatga             >  2:3700654/1‑150 (MQ=255)
                                                                                                                                        cAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAg            <  1:3228548/150‑1 (MQ=255)
                                                                                                                                         aGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAgg           <  2:218429/150‑1 (MQ=255)
                                                                                                                                             tctcACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgccag       <  2:4225062/150‑1 (MQ=255)
                                                                                                                                              ctcACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgccagc      >  2:3946692/1‑150 (MQ=255)
                                                                                                                                              ctcACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgccagc      >  2:3946590/1‑150 (MQ=255)
                                                                                                                                                cACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgccagcca    <  1:4086187/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCTTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGGCCAGCCATC  >  CP009273/4470726‑4471021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑