Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,482,573 A→T 14.9% L199Q (CTG→CAG)  idnO ← 5‑keto‑D‑gluconate‑5‑reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,482,5730AT14.9% 673.1 / 96.8 235L199Q (CTG→CAG) idnO5‑keto‑D‑gluconate‑5‑reductase
Reads supporting (aligned to +/- strand):  ref base A (84/116);  new base T (21/14);  total (105/130)
Fisher's exact test for biased strand distribution p-value = 6.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 241 total aligned reads displayed.

CGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACGTCCCAGT  >  CP009273/4482424‑4482722
                                                                                                                                                     |                                                                                                                                                     
cgccATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCa                                                                                                                                                       <  1:2229925/150‑1 (MQ=255)
 gccATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAg                                                                                                                                                      <  2:3093701/150‑1 (MQ=255)
    aTCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGc                                                                                                                                                   <  1:1733749/150‑1 (MQ=255)
      caacaaACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCtt                                                                                                                                                 <  1:2666111/150‑1 (MQ=255)
       aacaaacaACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCttt                                                                                                                                                <  1:2009989/150‑1 (MQ=255)
       aacaaacaACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCttt                                                                                                                                                <  2:2453043/150‑1 (MQ=255)
       aacaaacaACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCttt                                                                                                                                                <  2:2745777/150‑1 (MQ=255)
        acaaacaaCAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTa                                                                                                                                               <  2:4340747/150‑1 (MQ=255)
         caaacaaCAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAg                                                                                                                                              <  2:1103061/150‑1 (MQ=255)
          aaacaaCAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGt                                                                                                                                             <  2:1602090/150‑1 (MQ=255)
           aacaacAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTc                                                                                                                                            >  1:2851422/1‑150 (MQ=255)
              aacaGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCAtt                                                                                                                                         <  1:893967/150‑1 (MQ=255)
                caGGTGGCCGATTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCACTCAGTTCCTGCGGATCTCCCCAGCGAGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTc                                                                                                                                       <  2:3007741/150‑1 (MQ=255)
                 aGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCt                                                                                                                                      >  2:3359731/1‑150 (MQ=255)
                 aGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCt                                                                                                                                      <  1:1542055/150‑1 (MQ=255)
                   gTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGt                                                                                                                                    >  2:1440734/1‑150 (MQ=255)
                    tGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGtt                                                                                                                                   <  2:3488603/150‑1 (MQ=255)
                     ggCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAAAAAAGAAGCACCAATAATTTACAGCGAATATACCCAGAGTGAGGCGGGGGTACTATTGCACAACAAGGCTGTGAAGGCTTCTTAATAAACCTGTGATATAGTCAATTCTGttt                                                                                                                                  >  1:3593984/1‑150 (MQ=255)
                         gTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGaa                                                                                                                              <  2:2651058/150‑1 (MQ=255)
                         gTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGaa                                                                                                                              <  2:2651187/150‑1 (MQ=255)
                          tttACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGaaa                                                                                                                             >  2:3135488/1‑150 (MQ=255)
                           ttACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAAt                                                                                                                            >  1:1627651/1‑150 (MQ=255)
                           ttACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAAt                                                                                                                            <  1:805475/150‑1 (MQ=255)
                           ttACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTAATTTCTGTTTTGAAAt                                                                                                                            <  1:41630/150‑1 (MQ=255)
                             aCGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAg                                                                                                                          >  2:3694795/1‑150 (MQ=255)
                             aCGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAg                                                                                                                          <  2:922599/150‑1 (MQ=255)
                               gAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGcc                                                                                                                        <  1:3419011/150‑1 (MQ=255)
                                 aaTCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCg                                                                                                                      >  2:4626471/1‑150 (MQ=255)
                                 aaTCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCg                                                                                                                      >  1:3852704/1‑150 (MQ=255)
                                      gagGCTTTTGAAGAAAGGAACACCGCAGCACCACTCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCa                                                                                                                 <  1:3092490/150‑1 (MQ=255)
                                         gCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATa                                                                                                              >  1:2162703/1‑150 (MQ=255)
                                         gCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATa                                                                                                              <  2:2029986/150‑1 (MQ=255)
                                           ttttGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATAcc                                                                                                            <  1:541333/150‑1 (MQ=255)
                                           ttttGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATAcc                                                                                                            >  1:1776968/1‑150 (MQ=255)
                                            tttGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCg                                                                                                           >  2:1188034/1‑150 (MQ=255)
                                              tGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGtt                                                                                                         >  1:4432827/1‑150 (MQ=255)
                                               gaagaaAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTg                                                                                                        >  2:580689/1‑150 (MQ=255)
                                               gaagaaAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTg                                                                                                        <  2:444364/150‑1 (MQ=255)
                                                   aaaGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCt                                                                                                    >  2:4254267/1‑150 (MQ=255)
                                                    aaGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCACTACCGTTGACCTg                                                                                                   >  2:4162969/1‑150 (MQ=255)
                                                          cacCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATAtt                                                                                             <  2:3048958/150‑1 (MQ=255)
                                                           acCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATtg                                                                                            >  2:1456936/1‑150 (MQ=255)
                                                            ccGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATtgt                                                                                           <  1:2957401/150‑1 (MQ=255)
                                                             cGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATtgtg                                                                                          <  1:2802488/150‑1 (MQ=255)
                                                              gcagcaCCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTgg                                                                                         <  2:3299109/150‑1 (MQ=255)
                                                               cagcaCCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGgc                                                                                        <  1:2247761/150‑1 (MQ=255)
                                                                 gcaCCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGgcgc                                                                                      >  1:1879590/1‑150 (MQ=255)
                                                                    ccAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGcc                                                                                   <  2:4361572/150‑1 (MQ=255)
                                                                     cAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCa                                                                                  <  2:1356752/150‑1 (MQ=255)
                                                                      aaTCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAg                                                                                 <  1:1288036/150‑1 (MQ=255)
                                                                       aTCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGc                                                                                >  2:234344/1‑150 (MQ=255)
                                                                        tCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCt                                                                               >  1:231041/1‑150 (MQ=255)
                                                                          aGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCg                                                                             >  1:4371490/1‑150 (MQ=255)
                                                                          aGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCg                                                                             >  2:3883987/1‑150 (MQ=255)
                                                                           gTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGa                                                                            >  1:1116815/1‑150 (MQ=255)
                                                                                tGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGcac                                                                       <  2:296901/150‑1 (MQ=255)
                                                                                tGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGcac                                                                       >  1:2300775/1‑150 (MQ=255)
                                                                                  cGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACAt                                                                     <  1:217256/150‑1 (MQ=255)
                                                                                      tctcCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCcg                                                                 <  2:2561769/150‑1 (MQ=255)
                                                                                         ccccAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCgg                                                              >  2:1624459/1‑150 (MQ=255)
                                                                                          cccAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCggg                                                             >  1:1074848/1‑150 (MQ=255)
                                                                                          cccAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCggg                                                             <  2:4078260/150‑1 (MQ=255)
                                                                                           ccAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGt                                                            <  2:2861711/150‑1 (MQ=255)
                                                                                            cAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTg                                                           <  1:2517816/150‑1 (MQ=255)
                                                                                            cAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTg                                                           >  2:4559702/1‑150 (MQ=255)
                                                                                             aGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCACTACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGa                                                          >  1:3149467/1‑150 (MQ=255)
                                                                                               cGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGc                                                        >  1:3351444/1‑150 (MQ=255)
                                                                                                  gcggcgGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCAtt                                                     <  2:3198314/150‑1 (MQ=255)
                                                                                                   cggcggGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCAttt                                                    >  2:2884253/1‑150 (MQ=255)
                                                                                                              cGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCt                                         <  1:59668/150‑1 (MQ=255)
                                                                                                                 ttGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTc                                      <  2:590256/150‑1 (MQ=255)
                                                                                                                 ttGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTc                                      <  2:3672875/150‑1 (MQ=255)
                                                                                                                 ttGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTc                                      <  1:3253737/150‑1 (MQ=255)
                                                                                                                  tGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCTTTGACCTGAATATTGTTGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCg                                     <  2:3961706/150‑1 (MQ=255)
                                                                                                                  tGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCg                                     <  1:3759485/150‑1 (MQ=255)
                                                                                                                    cacaACCAGGCGGTGAATGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGAt                                   >  2:1013579/1‑150 (MQ=255)
                                                                                                                    cacaACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGAt                                   <  2:2217649/150‑1 (MQ=255)
                                                                                                                    cacaACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGAt                                   <  1:2255751/150‑1 (MQ=255)
                                                                                                                      caACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGc                                 >  1:622266/1‑150 (MQ=255)
                                                                                                                       aaCCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCg                                <  2:1856012/150‑1 (MQ=255)
                                                                                                                        aCCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCgg                               <  1:2714993/150‑1 (MQ=255)
                                                                                                                          cAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCa                             <  2:2111200/150‑1 (MQ=255)
                                                                                                                            ggCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATa                           >  2:1807834/1‑150 (MQ=255)
                                                                                                                              cGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAg                         >  1:3957966/1‑150 (MQ=255)
                                                                                                                               ggTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAgg                        >  1:531100/1‑150 (MQ=255)
                                                                                                                                gTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAggg                       <  1:2928170/150‑1 (MQ=255)
                                                                                                                                 tGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAgggg                      <  1:2213612/150‑1 (MQ=255)
                                                                                                                                 tGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAgggg                      <  1:2553794/150‑1 (MQ=255)
                                                                                                                                 tGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAgggg                      <  1:4541157/150‑1 (MQ=255)
                                                                                                                                       cTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATgg                <  1:3701958/150‑1 (MQ=255)
                                                                                                                                        ttCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGgt               <  1:2977362/150‑1 (MQ=255)
                                                                                                                                           gTCATCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTc            >  2:780591/1‑150 (MQ=255)
                                                                                                                                            tCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCa           <  2:4517819/150‑1 (MQ=255)
                                                                                                                                                cAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACGTc       <  1:1252385/150‑1 (MQ=255)
                                                                                                                                                cAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACGTc       >  1:710189/1‑150 (MQ=255)
                                                                                                                                                 aaCCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACGTcc      <  2:4441063/150‑1 (MQ=255)
                                                                                                                                                 aaCCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACGTcc      <  1:4457695/150‑1 (MQ=255)
                                                                                                                                                 aaCCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACGTcc      >  1:3278408/1‑150 (MQ=255)
                                                                                                                                                 aaCCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACGTcc      <  1:2130367/150‑1 (MQ=255)
                                                                                                                                                     aGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACGTCCCAGt  <  2:4289189/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACGTCCCAGT  >  CP009273/4482424‑4482722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑