Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,494,721 G→A 91.9% A283T (GCC→ACC)  yjhB → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,494,7210GA91.9% 933.0 / 57.3 298A283T (GCC→ACC) yjhBputative transporter
Reads supporting (aligned to +/- strand):  ref base G (11/13);  new base A (138/135);  total (149/149)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

Only 100 of 302 total aligned reads displayed.

CCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTATATA  >  CP009273/4494574‑4494869
                                                                                                                                                   |                                                                                                                                                     
 cGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCt                                                                                                                                                    <  1:1172513/150‑1 (MQ=255)
 cGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCt                                                                                                                                                    <  1:2594940/150‑1 (MQ=255)
   aTAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCttt                                                                                                                                                  >  2:1020106/1‑150 (MQ=255)
     aaaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGt                                                                                                                                                <  2:4620875/150‑1 (MQ=255)
      aaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCGTTGTa                                                                                                                                               >  2:719577/1‑150 (MQ=255)
        cGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGt                                                                                                                                             <  1:2864143/150‑1 (MQ=255)
            ctactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGt                                                                                                                                         <  1:2114/150‑1 (MQ=255)
            ctactTCCTTCCTACCTGGCAGATAATGGAGTTAAGACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGt                                                                                                                                         <  1:2200/150‑1 (MQ=255)
              actTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCt                                                                                                                                       <  1:1317291/150‑1 (MQ=255)
               cttccttcCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCta                                                                                                                                      <  2:1235782/150‑1 (MQ=255)
                  ccttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCtaata                                                                                                                                   <  2:3024821/150‑1 (MQ=255)
                       ctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTc                                                                                                                              >  1:105094/1‑150 (MQ=255)
                       ctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTc                                                                                                                              <  1:4497573/150‑1 (MQ=255)
                        tACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCa                                                                                                                             <  1:1447122/150‑1 (MQ=255)
                            tGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCattta                                                                                                                         <  1:60766/150‑1 (MQ=255)
                             ggCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCatttat                                                                                                                        <  1:4178618/150‑1 (MQ=255)
                              gCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCatttatt                                                                                                                       <  2:1341692/150‑1 (MQ=255)
                                 gATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTc                                                                                                                    >  1:3830177/1‑150 (MQ=255)
                                  aTAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTcc                                                                                                                   <  2:116086/150‑1 (MQ=255)
                                   tAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCt                                                                                                                  >  2:665396/1‑150 (MQ=255)
                                   tAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGAAAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTAATTTATTTTCCt                                                                                                                  >  2:1814812/1‑150 (MQ=255)
                                   tAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATGTTTTGGTTTTGGTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCt                                                                                                                  >  2:1268693/1‑150 (MQ=255)
                                    aaTGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCtt                                                                                                                 <  1:3105099/150‑1 (MQ=255)
                                      tGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTg                                                                                                               >  1:3020285/1‑150 (MQ=255)
                                      tGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTg                                                                                                               >  1:705612/1‑150 (MQ=255)
                                       ggAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGt                                                                                                              <  1:1650488/150‑1 (MQ=255)
                                        gAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTc                                                                                                             <  1:1908010/150‑1 (MQ=255)
                                         aGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTcc                                                                                                            >  1:3577559/1‑150 (MQ=255)
                                            tAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAGCTTCATTTATTTTCCTTTGTCctc‑t                                                                                                        >  1:4068082/1‑150 (MQ=255)
                                                aCAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑ttttt                                                                                                    >  1:4058787/1‑150 (MQ=255)
                                                  aGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑ttttttt                                                                                                  >  2:3332658/1‑150 (MQ=255)
                                                   gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑tttttttt                                                                                                 <  2:1473383/150‑1 (MQ=255)
                                                       tCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTAttt                                                                                             >  1:3522838/1‑150 (MQ=255)
                                                        cATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTc                                                                                            >  2:3403337/1‑150 (MQ=255)
                                                           ttCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTTATTTCtg                                                                                          >  2:2914288/1‑150 (MQ=255)
                                                            tCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCtgtg                                                                                        <  1:1225174/150‑1 (MQ=255)
                                                             cAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGa                                                                                       <  2:3828186/150‑1 (MQ=255)
                                                              aaCTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGaa                                                                                      >  1:3197705/1‑150 (MQ=255)
                                                                ctctGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGaaaa                                                                                    >  1:3432924/1‑150 (MQ=255)
                                                                 tctGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGaaaaa                                                                                   >  2:3702614/1‑150 (MQ=255)
                                                                  ctGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAAc                                                                                  >  1:4269681/1‑150 (MQ=255)
                                                                   tgatgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAAct                                                                                 >  1:402628/1‑150 (MQ=255)
                                                                   tgatgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAAct                                                                                 >  1:94101/1‑150 (MQ=255)
                                                                    gatgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAActc                                                                                >  2:808997/1‑150 (MQ=255)
                                                                      tgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAActctt                                                                              >  2:4116418/1‑150 (MQ=255)
                                                                      tgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAActctt                                                                              <  1:3769592/150‑1 (MQ=255)
                                                                       gaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAActcttc                                                                             <  2:3951451/150‑1 (MQ=255)
                                                                       gaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAActcttc                                                                             <  1:2084724/150‑1 (MQ=255)
                                                                          aaTAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAActcttctct                                                                          <  2:1794165/150‑1 (MQ=255)
                                                                           aTAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTTATTTCTGTGAAAAActcttctct                                                                          <  2:1925367/150‑1 (MQ=255)
                                                                              gCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTtata                                                                      <  1:3460462/150‑1 (MQ=255)
                                                                               cAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAg                                                                     >  1:3036408/1‑150 (MQ=255)
                                                                                aGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAgg                                                                    <  1:1996075/150‑1 (MQ=255)
                                                                                     tAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTAt                                                               >  2:2043218/1‑150 (MQ=255)
                                                                                      aGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATg                                                              <  2:2531712/150‑1 (MQ=255)
                                                                                      aGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATg                                                              >  1:1136296/1‑150 (MQ=255)
                                                                                       ggAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGt                                                             <  2:2749423/150‑1 (MQ=255)
                                                                                        gAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtc                                                            >  1:644233/1‑150 (MQ=255)
                                                                                        gAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtc                                                            >  2:1379622/1‑150 (MQ=255)
                                                                                            acTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCtt                                                        >  1:4404023/1‑150 (MQ=255)
                                                                                             cTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCttt                                                       <  1:3274850/150‑1 (MQ=255)
                                                                                             cTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCttt                                                       >  1:458185/1‑150 (MQ=255)
                                                                                              tGACAGGTACAATATTTTGTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTg                                                      <  2:3125324/150‑1 (MQ=255)
                                                                                               gACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTgg                                                     <  2:3223900/150‑1 (MQ=255)
                                                                                                 cAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGAt                                                   >  1:1953801/1‑150 (MQ=255)
                                                                                                    gTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTaa                                                <  2:907527/150‑1 (MQ=255)
                                                                                                     tACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTATTCGGTATAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTATGTGAAAAAATCTTCTCTTATAGGATTATTTCTCTTTGGATTAAt                                               >  1:206035/1‑150 (MQ=255)
                                                                                                      aCAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATg                                              >  2:4326860/1‑150 (MQ=255)
                                                                                                        aaTATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGtt                                            >  2:4253135/1‑150 (MQ=255)
                                                                                                        aaTATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCATC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGtt                                            >  2:3991479/1‑150 (MQ=255)
                                                                                                          tatTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTa                                          <  2:2096030/150‑1 (MQ=255)
                                                                                                              ttttGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACaaa                                      >  1:642135/1‑150 (MQ=255)
                                                                                                                 tGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATACCTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAAttt                                   <  2:3536037/150‑1 (MQ=255)
                                                                                                                  ggTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTa                                  >  1:3235987/1‑150 (MQ=255)
                                                                                                                  ggTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTa                                  >  1:2636722/1‑150 (MQ=255)
                                                                                                                   gTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAg                                 <  2:2168497/150‑1 (MQ=255)
                                                                                                                     tttGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTTTCTTTGGATTAATGTTTACAAATTTAGGt                               <  2:295481/150‑1 (MQ=255)
                                                                                                                     tttGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTTTCTTTGGATTAATGTTTACAAATTTAGGt                               <  2:4458929/150‑1 (MQ=255)
                                                                                                                     tttGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGt                               <  2:2140661/150‑1 (MQ=255)
                                                                                                                      ttgttgGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTa                              >  2:2099433/1‑150 (MQ=255)
                                                                                                                        gttgGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAtt                            >  1:3346714/1‑150 (MQ=255)
                                                                                                                        gttgGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAtt                            >  1:3176194/1‑150 (MQ=255)
                                                                                                                        gttgGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAtt                            <  2:4552647/150‑1 (MQ=255)
                                                                                                                        gttgGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAtt                            <  1:1798285/150‑1 (MQ=255)
                                                                                                                         ttgGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTg                           <  1:2360991/150‑1 (MQ=255)
                                                                                                                           ggTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCa                         >  1:769081/1‑150 (MQ=255)
                                                                                                                           ggTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCa                         >  1:1742024/1‑150 (MQ=255)
                                                                                                                               aTAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggt                     >  2:4088937/1‑150 (MQ=255)
                                                                                                                                tAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggtt                    <  2:3655624/150‑1 (MQ=255)
                                                                                                                                tAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggtt                    <  2:1610784/150‑1 (MQ=255)
                                                                                                                                  aGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttgg                  <  1:2824031/150‑1 (MQ=255)
                                                                                                                                  aGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttgg                  >  1:2659494/1‑150 (MQ=255)
                                                                                                                                  aGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttgg                  >  1:4595984/1‑150 (MQ=255)
                                                                                                                                   gATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGTTTAATGTTTACAAATTTAGGTATTGCAGggttggt                 >  2:3666793/1‑150 (MQ=255)
                                                                                                                                       ggTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTATGTATTGCAGGGTTGGTTCCa             >  2:2611936/1‑150 (MQ=255)
                                                                                                                                       ggTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCa             >  2:1665049/1‑150 (MQ=255)
                                                                                                                                        gtgtAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCaa            <  1:3972078/150‑1 (MQ=255)
                                                                                                                                              aaaaaaCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTta      >  1:2418156/1‑150 (MQ=255)
                                                                                                                                                aaaaCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTtata    <  2:2244184/150‑1 (MQ=255)
                                                                                                                                                  aGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTtatata  >  2:119599/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTATATA  >  CP009273/4494574‑4494869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑