Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,495,859 T→G 6.8% D252E (GAT→GAG)  yjhC → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,495,8590TG 6.8% 654.9 / 39.2 221D252E (GAT→GAG) yjhCputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (100/106);  new base G (9/6);  total (109/112)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 228 total aligned reads displayed.

GGTCCAGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAA  >  CP009273/4495710‑4496006
                                                                                                                                                     |                                                                                                                                                   
  tCCAGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGatat                                                                                                                                                   <  1:4256709/150‑1 (MQ=255)
  tCCAGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGatat                                                                                                                                                   <  1:3570543/150‑1 (MQ=255)
     aGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCa                                                                                                                                                >  2:960388/1‑150 (MQ=255)
     aGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCa                                                                                                                                                >  1:1057075/1‑150 (MQ=255)
     aGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCa                                                                                                                                                >  1:1623622/1‑150 (MQ=255)
      ggATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGATTTCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCaa                                                                                                                                               >  1:1123103/1‑150 (MQ=255)
        aTTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGa                                                                                                                                             >  1:3374742/1‑150 (MQ=255)
             gCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAAcag                                                                                                                                        <  1:3722888/150‑1 (MQ=255)
              cAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAAcagc                                                                                                                                       <  2:1451746/150‑1 (MQ=255)
               aaTGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAAcagca                                                                                                                                      <  2:1141847/150‑1 (MQ=255)
               aaTGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAAcagca                                                                                                                                      >  2:1643222/1‑150 (MQ=255)
               aaTGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAAcagca                                                                                                                                      >  1:2820958/1‑150 (MQ=255)
                   aaGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGt                                                                                                                                  >  1:4194399/1‑150 (MQ=255)
                    aGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGACCTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTc                                                                                                                                 >  1:887661/1‑150 (MQ=255)
                     gatgatATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCa                                                                                                                                <  1:48874/150‑1 (MQ=255)
                       tgatATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACt                                                                                                                              <  2:2118716/150‑1 (MQ=255)
                        gatATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACtt                                                                                                                             <  2:3209981/150‑1 (MQ=255)
                        gatATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACtt                                                                                                                             <  1:1208175/150‑1 (MQ=255)
                            tGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGa                                                                                                                         >  1:4310902/1‑150 (MQ=255)
                             gTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGAt                                                                                                                        <  2:1392293/150‑1 (MQ=255)
                              ttatttatGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGAtt                                                                                                                       <  2:3314930/150‑1 (MQ=255)
                               tatttatGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTg                                                                                                                      <  2:3631152/150‑1 (MQ=255)
                                atttatGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGTGTCACTTAGGATTgg                                                                                                                     >  2:1786885/1‑150 (MQ=255)
                                   tatGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCATAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCgg                                                                                                                  <  2:3057504/150‑1 (MQ=255)
                                      gACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCa                                                                                                               >  2:3512743/1‑150 (MQ=255)
                                      gACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCa                                                                                                               >  2:470414/1‑150 (MQ=255)
                                        ccTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGa                                                                                                             >  1:3710899/1‑150 (MQ=255)
                                        ccTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGa                                                                                                             >  2:4072122/1‑150 (MQ=255)
                                        ccTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGa                                                                                                             <  2:1890479/150‑1 (MQ=255)
                                          ttGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATGTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAGAGGCTCTATTAAAATTGATATGCAAGAAACAGCCGGGTCACTTAGGATTGGCGGTCAGACa                                                                                                           >  1:1392118/1‑150 (MQ=255)
                                           tGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACaa                                                                                                          <  2:3806881/150‑1 (MQ=255)
                                              aaTTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGc                                                                                                       >  2:1229348/1‑150 (MQ=255)
                                                ttCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAt                                                                                                     >  2:3444914/1‑150 (MQ=255)
                                                    cGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAttttt                                                                                                 >  1:3143637/1‑150 (MQ=255)
                                                    cGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAttttt                                                                                                 <  1:2902564/150‑1 (MQ=255)
                                                    cGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAttttt                                                                                                 <  1:2799927/150‑1 (MQ=255)
                                                     gTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAtttttt                                                                                                <  1:3483677/150‑1 (MQ=255)
                                                     gTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAtttttt                                                                                                <  2:1478695/150‑1 (MQ=255)
                                                      tCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTg                                                                                               <  1:2772432/150‑1 (MQ=255)
                                                       cAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTgg                                                                                              >  1:902836/1‑150 (MQ=255)
                                                        aGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGt                                                                                             <  1:2487726/150‑1 (MQ=255)
                                                         ggAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTAAACAGGCCGGAACATAAAGACATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATAGGCGGTCAGACAAAGCATTTTTAGGTc                                                                                            >  2:2060406/1‑150 (MQ=255)
                                                              aCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGa                                                                                       >  2:2163129/1‑150 (MQ=255)
                                                                 aGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAac                                                                                    <  1:1176690/150‑1 (MQ=255)
                                                                  gCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAaca                                                                                   <  2:3449741/150‑1 (MQ=255)
                                                                  gCAACCTTAGAGTGGGGGAGTGAATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAaca                                                                                   <  2:2964753/150‑1 (MQ=255)
                                                                     aCCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaa                                                                                >  1:2630921/1‑150 (MQ=255)
                                                                       cTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaaga                                                                              >  2:3556000/1‑150 (MQ=255)
                                                                         tAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaagaag                                                                            <  1:496400/150‑1 (MQ=255)
                                                                          agagTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaagaaga                                                                           >  1:2846295/1‑150 (MQ=255)
                                                                             gTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAagaagaaga                                                                        <  1:339410/150‑1 (MQ=255)
                                                                               gggggAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatg                                                                      >  1:1108845/1‑150 (MQ=255)
                                                                               gggggAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatg                                                                      >  1:2885942/1‑150 (MQ=255)
                                                                               gggggAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatg                                                                      >  1:3190168/1‑150 (MQ=255)
                                                                                ggggAGTGCATTTAACTGGCCGTAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatga                                                                     <  2:1800055/150‑1 (MQ=255)
                                                                                ggggAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatga                                                                     <  1:3884313/150‑1 (MQ=255)
                                                                                        cATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGAtcgtc                                                             <  1:4568392/150‑1 (MQ=255)
                                                                                          tttAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCgg                                                           <  2:4356270/150‑1 (MQ=255)
                                                                                             aaCTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGaaa                                                        >  2:2679559/1‑150 (MQ=255)
                                                                                              aCTGGCCGGAACATTATGTCATCATCAATGTAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAg                                                       <  1:3218267/150‑1 (MQ=255)
                                                                                              aCTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAg                                                       <  2:2598761/150‑1 (MQ=255)
                                                                                                   ccGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAat                                                  <  2:1213644/150‑1 (MQ=255)
                                                                                                    cGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTATGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAAAAGATGATGATCGGCGGAAAGGCAata                                                 >  1:4625491/1‑150 (MQ=255)
                                                                                                    cGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAata                                                 >  1:3606806/1‑150 (MQ=255)
                                                                                                      gAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATg                                               <  2:3655191/150‑1 (MQ=255)
                                                                                                      gAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATg                                               >  1:3230400/1‑150 (MQ=255)
                                                                                                          aTTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCt                                           >  1:3977144/1‑150 (MQ=255)
                                                                                                            tATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCa                                         <  1:209004/150‑1 (MQ=255)
                                                                                                            tATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCa                                         <  1:797268/150‑1 (MQ=255)
                                                                                                            tATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCa                                         >  1:164139/1‑150 (MQ=255)
                                                                                                             aTGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGATACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAg                                        <  1:375579/150‑1 (MQ=255)
                                                                                                               gTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGaa                                      <  2:3908425/150‑1 (MQ=255)
                                                                                                                tcatcatcaATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGaaa                                     <  1:1449853/150‑1 (MQ=255)
                                                                                                                 catcatcaATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAat                                    >  2:1079346/1‑150 (MQ=255)
                                                                                                                  atcatcaATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatg                                   <  2:1976372/150‑1 (MQ=255)
                                                                                                                  atcatcaATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCAATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAAAATGACCTCAGAAatg                                   >  1:570884/1‑150 (MQ=255)
                                                                                                                       caATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggatgg                              >  1:530782/1‑150 (MQ=255)
                                                                                                                        aaTGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAAAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGgc                             >  1:1288467/1‑150 (MQ=255)
                                                                                                                        aaTGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAAAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGgc                             >  1:358921/1‑150 (MQ=255)
                                                                                                                           ggAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCt                          <  1:4061982/150‑1 (MQ=255)
                                                                                                                           ggAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCt                          >  1:3071487/1‑150 (MQ=255)
                                                                                                                           ggAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCt                          <  1:1602464/150‑1 (MQ=255)
                                                                                                                             aaCTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCtat                        <  1:1977651/150‑1 (MQ=255)
                                                                                                                             aaCTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCtat                        >  1:4019707/1‑150 (MQ=255)
                                                                                                                               cTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAg                      >  2:3168751/1‑150 (MQ=255)
                                                                                                                               cTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAg                      >  2:850533/1‑150 (MQ=255)
                                                                                                                                     gCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATg                <  2:3034192/150‑1 (MQ=255)
                                                                                                                                      ctctATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATgg               >  1:2195075/1‑150 (MQ=255)
                                                                                                                                      ctctATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATgg               <  2:3029261/150‑1 (MQ=255)
                                                                                                                                        ctATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTc             <  2:3374742/150‑1 (MQ=255)
                                                                                                                                          aTTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCAt           <  1:4173133/150‑1 (MQ=255)
                                                                                                                                           ttAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATc          <  2:2458308/150‑1 (MQ=255)
                                                                                                                                           ttAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATc          <  2:759136/150‑1 (MQ=255)
                                                                                                                                              aaaTTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTACATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGTTCATCCAg       >  1:2116639/1‑150 (MQ=255)
                                                                                                                                               aaTTGATATTCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGACGGCGCTATAGCATATGGTCATCCAgg      <  2:4222452/150‑1 (MQ=255)
                                                                                                                                               aaTTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAgg      >  1:547736/1‑150 (MQ=255)
                                                                                                                                                aTTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGa     <  1:3445100/150‑1 (MQ=255)
                                                                                                                                                   gATATGCAAGAAACAGCAGGGTCACTTATGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGaaaa  >  1:1788860/1‑150 (MQ=255)
                                                                                                                                                   gATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGaaaa  >  2:3742663/1‑150 (MQ=255)
                                                                                                                                                   gATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGaaaa  >  2:4632109/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGTCCAGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAA  >  CP009273/4495710‑4496006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑