Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,514,180 A→T 11.5% A216A (GCT→GCA)  yjhH ← putative lyase/synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,514,1800AT11.5% 863.3 / 77.8 282A216A (GCT→GCA) yjhHputative lyase/synthase
Reads supporting (aligned to +/- strand):  ref base A (122/127);  new base T (11/22);  total (133/149)
Fisher's exact test for biased strand distribution p-value = 9.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

ACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGAT  >  CP009273/4514031‑4514329
                                                                                                                                                     |                                                                                                                                                     
 cacacTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAg                                                                                                                                                      <  1:3359721/150‑1 (MQ=255)
  acacTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGc                                                                                                                                                     >  1:3731251/1‑150 (MQ=255)
      tGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAg                                                                                                                                                 <  1:1061710/150‑1 (MQ=255)
      tGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAg                                                                                                                                                 >  1:504325/1‑150 (MQ=255)
        cATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGtt                                                                                                                                               >  2:4559764/1‑150 (MQ=255)
        cATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGtt                                                                                                                                               >  2:4491381/1‑150 (MQ=255)
        cATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGtt                                                                                                                                               <  2:3031700/150‑1 (MQ=255)
         aTGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTa                                                                                                                                              <  1:2999294/150‑1 (MQ=255)
             tGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAgcgc                                                                                                                                          >  1:1631802/1‑150 (MQ=255)
               tATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTg                                                                                                                                        <  1:1457784/150‑1 (MQ=255)
                 tttGATCAGTGAGTGAATCGGTGTTTCGAGGGCGTATATAGCGGGCAGTTGGTGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGGCTTCACGCCAGGCGCGGTAGTTGCCGTCGGAGTGTTCCGGTGCATAGTTAGCGCTGGc                                                                                                                                      <  2:4348995/150‑1 (MQ=255)
                  ttGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCg                                                                                                                                     <  2:1474665/150‑1 (MQ=255)
                   tGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCgg                                                                                                                                    >  2:1087488/1‑150 (MQ=255)
                    gATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGt                                                                                                                                   <  1:257136/150‑1 (MQ=255)
                      tCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTa                                                                                                                                 >  2:1697336/1‑150 (MQ=255)
                        aGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATc                                                                                                                               >  2:4099198/1‑150 (MQ=255)
                        aGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATc                                                                                                                               >  1:4352150/1‑150 (MQ=255)
                          tGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGc                                                                                                                             <  2:1892993/150‑1 (MQ=255)
                          tGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGc                                                                                                                             >  1:3505758/1‑150 (MQ=255)
                           gagaCAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCa                                                                                                                            <  2:720684/150‑1 (MQ=255)
                           gagaCAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCa                                                                                                                            <  1:4287835/150‑1 (MQ=255)
                                aaaCGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTc                                                                                                                       >  2:3866714/1‑150 (MQ=255)
                                   cGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTcgcc                                                                                                                    >  1:4322549/1‑150 (MQ=255)
                                   cGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTcgcc                                                                                                                    >  2:2810211/1‑150 (MQ=255)
                                         ttCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCcag                                                                                                              >  1:1799818/1‑150 (MQ=255)
                                           cGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCcagca                                                                                                            <  1:1205975/150‑1 (MQ=255)
                                            gAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCcagcag                                                                                                           <  2:1032927/150‑1 (MQ=255)
                                             aGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCagcagc                                                                                                          >  1:4197586/1‑150 (MQ=255)
                                              gggCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAgcagca                                                                                                         >  2:877254/1‑150 (MQ=255)
                                                    aaaTAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTAt                                                                                                   >  2:3840904/1‑150 (MQ=255)
                                                     taTAGCGGGCAGTTGTATTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGACTTAACGCCAGGCGCTGTAGATGCCGACGGAGAGTTCCGGTGCTAAGTTAGCGCTGGCGTTTATCGCACAGTCGCAGCCCAGAAGCATCGTAtt                                                                                                  <  2:530490/149‑1 (MQ=255)
                                                      aTAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTc                                                                                                 >  2:163168/1‑150 (MQ=255)
                                                            ggCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCaaa                                                                                           >  1:4250750/1‑150 (MQ=255)
                                                             gCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAAt                                                                                          <  2:2724608/150‑1 (MQ=255)
                                                               aGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGa                                                                                        >  2:3253704/1‑150 (MQ=255)
                                                               aGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGa                                                                                        <  2:1730153/150‑1 (MQ=255)
                                                                gTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGat                                                                                       <  2:1556450/150‑1 (MQ=255)
                                                                gTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGat                                                                                       <  1:596319/150‑1 (MQ=255)
                                                                gTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGat                                                                                       <  1:2302741/150‑1 (MQ=255)
                                                                gTTGAAGTAGTTATTTATTCAGCGTCGCAGCTGTCGCCAGATCGCCTTCACGCCATCCGCGGTAGATGCCGACCGAGACTTCCCGTGCAAAGTTAGCGCTGGCGGTTATCTCACCTTCCCCGCCCAACAGCATCGTATTCAGCACATGat                                                                                       >  1:2091812/1‑150 (MQ=255)
                                                                  tGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGatca                                                                                     >  1:2271770/1‑150 (MQ=255)
                                                                    tagtagTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGatcatc                                                                                   <  2:3966712/150‑1 (MQ=255)
                                                                     agtagtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCg                                                                                  <  1:3176827/150‑1 (MQ=255)
                                                                      gtagtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGt                                                                                 >  1:2487715/1‑150 (MQ=255)
                                                                      gtagtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCACCCAGCAGCATCGTATTCAGCAAATGATCATCGt                                                                                 <  1:939295/150‑1 (MQ=255)
                                                                           tttttATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCACATGATCAGCGTAACCg                                                                            >  1:1596785/1‑150 (MQ=255)
                                                                           tttttATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCg                                                                            >  1:3287636/1‑150 (MQ=255)
                                                                             tttATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCa                                                                          <  2:3418842/150‑1 (MQ=255)
                                                                              ttATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAg                                                                         <  1:392147/150‑1 (MQ=255)
                                                                               tATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGa                                                                        >  1:3797146/1‑150 (MQ=255)
                                                                                aTTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCTCTGGCTGTTATCGCACCGTCGCCGCCCAGCAGCATCTTATTCAGCAAATGATCATCGTAACCGCAGaa                                                                       <  2:2137927/150‑1 (MQ=255)
                                                                                  tCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCACATGATCATCGTAACCGCAGAATa                                                                     >  1:3397457/1‑150 (MQ=255)
                                                                                  tCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATa                                                                     <  2:750410/150‑1 (MQ=255)
                                                                                   cAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATAc                                                                    >  2:608972/1‑150 (MQ=255)
                                                                                   cAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATAc                                                                    >  2:233454/1‑150 (MQ=255)
                                                                                     gCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCg                                                                  <  2:967384/150‑1 (MQ=255)
                                                                                      cGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGa                                                                 >  2:3940897/1‑150 (MQ=255)
                                                                                      cGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGa                                                                 >  2:3600989/1‑150 (MQ=255)
                                                                                       gTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGaa                                                                <  1:3182069/150‑1 (MQ=255)
                                                                                       gTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGaa                                                                >  2:793510/1‑150 (MQ=255)
                                                                                       gTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGaa                                                                >  1:3381917/1‑150 (MQ=255)
                                                                                       gTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGaa                                                                >  1:1857377/1‑150 (MQ=255)
                                                                                           cAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacg                                                            >  1:1294604/1‑150 (MQ=255)
                                                                                           cAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacg                                                            <  2:2280748/150‑1 (MQ=255)
                                                                                           cAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacg                                                            >  1:3151886/1‑150 (MQ=255)
                                                                                           cAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacg                                                            <  2:2798057/150‑1 (MQ=255)
                                                                                              cGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacgacg                                                         >  2:3829662/1‑150 (MQ=255)
                                                                                               ggTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCCTCGTAACCGCAGAATACCGAAAACGACgg                                                        >  2:2878422/1‑150 (MQ=255)
                                                                                                  cGCCAGTTCGCCTTCACGCCAGGCGCGGTATATGCCGACGGATTGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACGGTCGCCGCCCAGCAGAATCGTATTCTGAAATTTATCATCGTACCCGCAGAATACCGAAAACGACGGGCg                                                     <  1:4106495/150‑1 (MQ=255)
                                                                                                   gCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGt                                                    >  2:4435204/1‑150 (MQ=255)
                                                                                                   gCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGt                                                    >  2:3799319/1‑150 (MQ=255)
                                                                                                   gCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGt                                                    >  1:3334730/1‑150 (MQ=255)
                                                                                                    ccAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAATTGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTa                                                   >  1:3249760/1‑150 (MQ=255)
                                                                                                     cAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTAc                                                  >  1:2639459/1‑150 (MQ=255)
                                                                                                      agagCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTAcc                                                 <  2:3477886/150‑1 (MQ=255)
                                                                                                       gATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCg                                                <  2:1839925/150‑1 (MQ=255)
                                                                                                       gATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCg                                                >  1:4135978/1‑150 (MQ=255)
                                                                                                       gATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCACACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCg                                                >  1:388737/1‑150 (MQ=255)
                                                                                                            ccTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTa                                           >  2:1852207/1‑150 (MQ=255)
                                                                                                               tCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTCGCGCTGGCGTTTATCCCCCCGTCGCCGCCCAGCAGAATCGTATTCAGCAAATGAACATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACt                                        <  1:3489565/150‑1 (MQ=255)
                                                                                                                cACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACtg                                       <  1:3629299/150‑1 (MQ=255)
                                                                                                                cACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACtg                                       >  1:1945501/1‑150 (MQ=255)
                                                                                                                 aCGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAACACGACGGGCGTACCGACTTAACtgt                                      <  1:1013058/150‑1 (MQ=255)
                                                                                                                     cAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTg                                  >  2:3722731/1‑150 (MQ=255)
                                                                                                                      aGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGa                                 >  1:881495/1‑150 (MQ=255)
                                                                                                                       ggCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCCAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGat                                >  1:272242/1‑150 (MQ=255)
                                                                                                                         cgcgGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGatca                              >  1:1998502/1‑150 (MQ=255)
                                                                                                                           cgGTAGAGGCCGACGGAGAGTTCCGGAGCAAAGTTCGCGCGGGCGTTTATCGCACCGTCGCCGCCCCGCAGCATCGTTTTCACCAAAGGATAATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGatcatc                            <  1:766419/150‑1 (MQ=255)
                                                                                                                              tAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTa                         >  1:3472521/1‑150 (MQ=255)
                                                                                                                                   gCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCaa                    <  1:2936260/150‑1 (MQ=255)
                                                                                                                                     cGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGt                  <  2:2951332/150‑1 (MQ=255)
                                                                                                                                         ggAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGAcc              >  2:2553307/1‑150 (MQ=255)
                                                                                                                                          gagaGTTCCGGAGAAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCa             <  1:84049/150‑1 (MQ=255)
                                                                                                                                            gagTTCCGGAGCAAAGTTAGCGCGGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAAc           <  2:830533/150‑1 (MQ=255)
                                                                                                                                             agTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCACATGAACATCGTAACCGCAGAATACCGAAAACGACGGGCGAACCGACAAAACTGTGTAGATCATCGTACGCAAGTGACCAACg          >  1:1289532/1‑150 (MQ=255)
                                                                                                                                                tCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTg       <  2:944585/150‑1 (MQ=255)
                                                                                                                                                 ccGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGt      <  2:4123405/150‑1 (MQ=255)
                                                                                                                                                  cGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTc     <  2:4555745/150‑1 (MQ=255)
                                                                                                                                                  cGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTc     <  2:1839586/150‑1 (MQ=255)
                                                                                                                                                   ggAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCg    >  2:4455502/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGAT  >  CP009273/4514031‑4514329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑