BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,596,008	C→A	46.2%	intergenic (‑26/+9)	leuV ← / ← leuP	tRNA‑Leu/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,596,008	0	C	A	46.2%	139.3 / 225.1	200	intergenic (‑26/+9)	leuV/leuP	tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base C (42/64); new base A (41/48); total (86/114)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 204 total aligned reads displayed.

AAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCC  >  CP009273/4595859‑4596157

aaaTCAAGGACGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATc                                                                                                                                                        <  1:3324097/150‑1 (MQ=255)

aaaTCAAGGAAGAAACAAGAACGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATc                                                                                                                                                        <  1:4419938/150‑1 (MQ=255)

 aaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCg                                                                                                                                                       <  1:3166375/150‑1 (MQ=255)

  aTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGc                                                                                                                                                      <  1:1944212/150‑1 (MQ=255)

  aTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                                                                                                                                                      >  1:3740551/1‑150 (MQ=255)

  aTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGc                                                                                                                                                      <  1:4507560/150‑1 (MQ=255)

    cAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCt                                                                                                                                                    <  2:1388061/150‑1 (MQ=255)

     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGACATCTTACTTTATTTGATATCgacac                                                                                                                                                   >  2:3273944/1‑146 (MQ=255)

      aGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCg                                                                                                                                                  <  2:858793/150‑1 (MQ=255)

       ggAAGAAAAAAGAATGGTAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGt                                                                                                                                                 <  1:1069442/150‑1 (MQ=255)

         aagaaACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTAGATATCTCATCGttt                                                                                                                                               >  2:2772363/1‑150 (MQ=255)

           gaaACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTgg                                                                                                                                             <  2:1522797/150‑1 (MQ=255)

            aaaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGt                                                                                                                                            <  2:1484295/150‑1 (MQ=255)

             aaCAAGAAAGGAAGTAAAGATAATTGGTGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg                                                                                                                                           <  1:1289347/150‑1 (MQ=255)

               cAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGGCAAATTACTTTATTTGATATAGACTCGATTGGTGC‑g                                                                                                                                        >  2:21563/1‑150 (MQ=18)

                  gAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAgg                                                                                                                                     >  1:1702336/1‑150 (MQ=255)

                       gAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGACATCATACTTTTTATGATATTGCCACGATTGGTGC‑GAgtggggg                                                                                                                                >  1:3068311/1‑150 (MQ=18)

                               gATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGaa                                                                                                                        <  2:4537302/150‑1 (MQ=255)

                               gATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGaa                                                                                                                        <  1:2683232/150‑1 (MQ=255)

                                      ggTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCAc                                                                                                                 <  1:2357004/150‑1 (MQ=255)

                                      ggTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCAc                                                                                                                 <  1:2132147/150‑1 (MQ=255)

                                       gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACg                                                                                                                <  1:348310/150‑1 (MQ=255)

                                          cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTTATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTcc                                                                                                             <  1:144698/150‑1 (MQ=255)

                                          cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTcc                                                                                                             <  1:655623/150‑1 (MQ=255)

                                           gAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCg                                                                                                            <  1:3070042/150‑1 (MQ=255)

                                                gtgggTCTAGAACCCCCACTTCATTAAGTACACTAACACATGAAGATATCTCGTCTACCTATTCAGCCACCTACGCACAGTCATCTAAATTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGa                                                                                                       <  2:1818369/148‑1 (MQ=25)

                                                gggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTNAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAANCCCCACGTCCGTAAgn                                                                                                       <  2:1818480/150‑2 (MQ=255)

                                                   ttaCTTTAACCCCCACGTCCTTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAaca                                                                                                    <  1:1178466/148‑1 (MQ=255)

                                                   ggACTTGAACCCCCACGTCCTTAAGGACACTAACACCTGAAGCTAGCGCGTATACCAATTCCGCCACCTTCCAACAGTCATCTTACTTTTTTTGATTTAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAaca                                                                                                    <  2:814518/150‑1 (MQ=25)

                                                   ggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAaca                                                                                                    <  1:1916957/150‑1 (MQ=255)

                                                     aCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGACTCGGGTGGTGC‑GAGTGGGGGGAGTTGAACCCCCACGTGCGTAAGAtcaca                                                                                                  >  2:2533496/1‑149 (MQ=21)

                                                      cTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTa                                                                                                 <  2:2765435/150‑1 (MQ=255)

                                                      cTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTa                                                                                                 >  1:956451/1‑150 (MQ=255)

                                                       ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTAATTGATATAGCATCTGTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACaaa                                                                                                >  1:3519318/1‑150 (MQ=25)

                                                         gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAaca                                                                                              >  1:2784448/1‑150 (MQ=255)

                                                         gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGGAAGAACACTAaca                                                                                              >  1:2784423/1‑150 (MQ=255)

                                                           aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                            >  1:3277809/1‑150 (MQ=255)

                                                           aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                            >  1:1785670/1‑150 (MQ=255)

                                                           aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAGCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                            >  2:1483060/1‑150 (MQ=255)

                                                            cccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                                                           <  1:4465270/150‑1 (MQ=255)

                                                             ccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                                                          <  1:4358629/150‑1 (MQ=255)

                                                              cccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                                                         <  2:4101717/150‑1 (MQ=255)

                                                              cccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAGATTACTTGGTTTGAGATAGCCTCGGATGGTGG‑GAGGGGGGGGACGTGAACCACCACGTCCGGAAGAACACAAACACCTgg                                                                                         >  2:578759/1‑149 (MQ=21)

                                                              cccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAACAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCTTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                                                         <  2:1146113/150‑1 (MQ=255)

                                                                    tCCGTAAGGACACTAACACCTGAAGCTATCTCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCTTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                                   <  2:2706958/150‑1 (MQ=255)

                                                                     ccttattgACACTTACACATGATGATAGCGCTTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATTTAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                                                                  <  2:1226932/143‑1 (MQ=25)

                                                                        tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGGCAGCTTACTTTTTTTGATATAGCATCGTTTGGTGC‑GAGGCGGGGGACTTGAACCACCACGTCCGTAAGAACACAAACACCTGAAGCTAgatcg                                                                               >  2:3607863/1‑146 (MQ=25)

                                                                         aaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                                                              >  2:167421/1‑150 (MQ=255)

                                                                          aGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                                                             >  2:3400294/1‑150 (MQ=255)

                                                                            gACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                                                           <  2:2409423/150‑1 (MQ=255)

                                                                             acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                                                          <  2:956451/150‑1 (MQ=255)

                                                                               acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                        >  1:4338727/1‑150 (MQ=255)

                                                                                cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                                       >  2:2274604/1‑150 (MQ=255)

                                                                                     acCTGATGCTAGCCCGTATAGAATTTCCGCGACCTTCTCACAGTCATTTTACTTTTTTGGATATTGGCTCTTTTGGTGC‑GAGGGGGGGGACTTGAACCACCACTTGAGTACGTACACTCACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                  <  2:1823607/150‑1 (MQ=12)

                                                                                     acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATTACTTTAAAAGATTTCGGATCGTTTTGTGC‑GAGGGGGGGGACTTGAACCACCACTTCCATAAGAACACAAACACCTTAAGCTAGAACGTATACCaaaacct                                                                  >  1:2449595/1‑145 (MQ=18)

                                                                                      ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAGCTTACTTTAAAAGATATGGAAGCTGTTTGTGC‑GAGGGGGGGGACTTTAACCACCACGTCCGTACGAACACAAAAACCTGGAGCTAGAGCGAATACCAAAACCGc                                                                 >  2:3199291/1‑150 (MQ=12)

                                                                                          aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                             >  1:3372097/1‑150 (MQ=255)

                                                                                          aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                             >  1:1638436/1‑150 (MQ=255)

                                                                                          aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGACAGCTTACTTTTAATGATATTGACGCGAGTGGGGC‑GAGGGGGGGGACTTGCACCCCCACGTCCGTAGGTAAACTATCACCTGAGGCTAGCGCGTCTACCGATTCCGCCAcc                                                             >  2:3470529/1‑150 (MQ=18)

                                                                                            gCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCGAGCGCGTCTACCAATTCCGCCACCtt                                                           <  2:1762043/150‑1 (MQ=255)

                                                                                             cgaGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATTTAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACATGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                          <  2:4373172/148‑1 (MQ=255)

                                                                                             cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCTTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                          <  2:3414223/150‑1 (MQ=255)

                                                                                             cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                          <  1:1149820/150‑1 (MQ=255)

                                                                                             cTAGCGCGTCTACCAATTCCGCCACCTTCGAACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                          <  2:3919201/150‑1 (MQ=255)

                                                                                             cTAGCGCGTATACCAATTCCGCCACCTTCGAACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                          <  2:4071056/150‑1 (MQ=255)

                                                                                                    gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                   >  2:4272418/1‑150 (MQ=255)

                                                                                                     ttttacAAATCCGGCAACTTCGCACATTGATCTTAATTTTTTTGTTTTTGCCTCGTTTGGTGCGGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                   <  2:4198183/145‑1 (MQ=17)

                                                                                                       tACCATTTCCGCCACCTTCGAACAGTAATCTTACTTTTTTTGTTATAGCCTCTTTTGGTGC‑GTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                <  1:607162/150‑1 (MQ=18)

                                                                                                         ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                              >  1:3674411/1‑150 (MQ=255)

                                                                                                          cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAgagcgagc                                             >  1:1218146/1‑150 (MQ=255)

                                                                                                          cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACACTGCGAGc                                             >  2:1432193/1‑150 (MQ=255)

                                                                                                              tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACACAGCGAGCAata                                         >  2:3461466/1‑150 (MQ=255)

                                                                                                               ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATCTCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAatat                                        <  2:2724856/150‑1 (MQ=255)

                                                                                                               ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAAAGAGCGAGGAatat                                        >  2:3955163/1‑150 (MQ=21)

                                                                                                                 gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa                                      <  1:4074134/150‑1 (MQ=255)

                                                                                                                 gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa                                      >  1:701817/1‑150 (MQ=255)

                                                                                                                 gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATCa                                      >  1:3924309/1‑150 (MQ=255)

                                                                                                                 gCCAACTTAGCACATTCATGTTACTTTTTTTGATCTATCCTAGATTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa                                      <  2:1911277/150‑1 (MQ=18)

                                                                                                                       ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTgg                                >  2:4229641/1‑150 (MQ=255)

                                                                                                                       ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTgg                                >  1:4236303/1‑150 (MQ=255)

                                                                                                                        tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGt                               >  2:3306347/1‑150 (MQ=255)

                                                                                                                         cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCNTCGCCCAGTGCGAGCAATATCAACGTGGtt                              <  1:4275702/150‑1 (MQ=255)

                                                                                                                         cGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtt                              >  1:246696/1‑150 (MQ=255)

                                                                                                                         atatcattCAACTTACTATTATTGATTTATACTTTTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtt                              <  2:2644010/143‑1 (MQ=17)

                                                                                                                           cacaTTCATGTTACATTTTTTGATTTAGAGTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt                            <  1:1477113/150‑1 (MQ=18)

                                                                                                                           cacaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAGCAACGTGGtttt                            >  1:3479919/1‑150 (MQ=255)

                                                                                                                            acaGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttttt                           <  1:153678/150‑1 (MQ=255)

                                                                                                                             caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGGGCGAGCAATATCAACGTGGTTTTTg                          >  1:777472/1‑150 (MQ=255)

                                                                                                                              aGTCATCTTACTTTTTTTGATCTATCCTCCTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                         <  2:304825/150‑1 (MQ=255)

                                                                                                                                tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGTg                       >  2:2930329/1‑150 (MQ=255)

                                                                                                                                tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                       <  2:877693/150‑1 (MQ=255)

                                                                                                                                   tCTTACTTTTTTTGATATCGCGTCGTTTGGTGC‑TAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                    <  1:274580/150‑1 (MQ=255)

                                                                                                                                   tCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATCAACGTGGATTTTGGTGCGa                    >  2:3767980/1‑150 (MQ=255)

                                                                                                                                   tCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                    >  1:526792/1‑150 (MQ=255)

                                                                                                                                        cTTTTTTTGATATAGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg               >  2:3637062/1‑150 (MQ=255)

                                                                                                                                            ttttGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa           <  2:3731211/150‑1 (MQ=255)

                                                                                                                                                gATATAGCGTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTg       <  2:132507/150‑1 (MQ=255)

                                                                                                                                                 atatCTCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGa      >  1:188808/1‑150 (MQ=255)

                                                                                                                                                 atatCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGa      >  1:2092527/1‑150 (MQ=255)

                                                                                                                                                    tCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAATTGAAcc   >  2:1099417/1‑150 (MQ=255)

AAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCC  >  CP009273/4595859‑4596157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`