Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 411,027 G→T 100% L61L (CTC→CTA)  sbcC ← exonuclease, dsDNA, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273411,0270GT96.9% 829.1 / 16.1 255L61L (CTC→CTA) sbcCexonuclease, dsDNA, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (3/5);  new base T (111/136);  total (114/141)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 260 total aligned reads displayed.

CAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGA  >  CP009273/410883‑411173
                                                                                                                                                |                                                                                                                                                  
cAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGAcg                                                                                                                                               >  1:3471649/1‑150 (MQ=255)
cAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGAcg                                                                                                                                               >  1:3471822/1‑150 (MQ=255)
cAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGAcg                                                                                                                                               <  1:544144/150‑1 (MQ=255)
cAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCAGGAGATCATTTTGCGATTGTGAAACGTTAGATAGAcg                                                                                                                                               >  1:1986414/1‑150 (MQ=255)
  aaTTACCGTCGGGTTGGTTACGCGCCCGAGTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGAcgcg                                                                                                                                             <  2:4454650/150‑1 (MQ=255)
   aTTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCgg                                                                                                                                            <  2:2074287/150‑1 (MQ=255)
    ttACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGa                                                                                                                                           >  1:1307312/1‑150 (MQ=255)
        cGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGttt                                                                                                                                       <  1:1938080/150‑1 (MQ=255)
        cGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGttt                                                                                                                                       >  2:397929/1‑150 (MQ=255)
           cGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGt                                                                                                                                    >  2:3801920/1‑150 (MQ=255)
           cGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGt                                                                                                                                    <  2:3456021/150‑1 (MQ=255)
            gggTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTg                                                                                                                                   >  1:3381425/1‑150 (MQ=255)
              gttggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGAt                                                                                                                                 <  2:2930291/150‑1 (MQ=255)
              gttggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTGTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGAt                                                                                                                                 <  2:1273231/150‑1 (MQ=255)
                  gttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAg                                                                                                                             >  2:1550580/1‑150 (MQ=255)
                   ttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAgc                                                                                                                            <  2:1163241/150‑1 (MQ=255)
                    tACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGGTTCGTGATACAgcg                                                                                                                           >  2:560489/1‑150 (MQ=255)
                      cgcgCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGcc                                                                                                                         >  2:2255217/1‑150 (MQ=255)
                       gcgcCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCa                                                                                                                        <  2:1994195/150‑1 (MQ=255)
                       gcgcCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCa                                                                                                                        <  2:3177522/150‑1 (MQ=255)
                        cgcCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAg                                                                                                                       >  1:4431167/1‑150 (MQ=255)
                        cgcCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAg                                                                                                                       <  2:4447176/150‑1 (MQ=255)
                            cGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACaa                                                                                                                   <  1:3038026/150‑1 (MQ=255)
                            cGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACaa                                                                                                                   >  2:1611952/1‑150 (MQ=255)
                             gATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAATCGTTAGATAGACGCGGAGTTTCGTGAGACAGCGCCAGACact                                                                                                                  >  2:67641/1‑148 (MQ=255)
                             gATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACaaa                                                                                                                  >  1:2884150/1‑150 (MQ=255)
                                tCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATgg                                                                                                               <  1:1844929/150‑1 (MQ=255)
                                     cTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTcc                                                                                                          >  2:4536054/1‑150 (MQ=255)
                                     cTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTcc                                                                                                          >  2:2207868/1‑150 (MQ=255)
                                     cTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTcc                                                                                                          <  1:1815581/150‑1 (MQ=255)
                                     cTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTCTCGTGATACAGCGCCAGACAAATGGCGTcc                                                                                                          <  1:4285412/150‑1 (MQ=255)
                                       ccAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCcag                                                                                                        <  1:3340160/150‑1 (MQ=255)
                                            aTGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAggg                                                                                                   >  1:3140233/1‑150 (MQ=255)
                                            aTGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAggg                                                                                                   >  1:4134832/1‑150 (MQ=255)
                                               cACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGggtgg                                                                                                <  2:3264115/150‑1 (MQ=255)
                                                aCGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGggtggt                                                                                               >  2:3196114/1‑150 (MQ=255)
                                                 cGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGtt                                                                                              >  1:2047330/1‑150 (MQ=255)
                                                  ggTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGttt                                                                                             >  2:2983476/1‑150 (MQ=255)
                                                   gTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGtttt                                                                                            <  1:2945685/150‑1 (MQ=255)
                                                     aCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTcc                                                                                          <  2:934349/150‑1 (MQ=255)
                                                       gCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTcccc                                                                                        >  1:689366/1‑150 (MQ=255)
                                                         ttCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATGTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGc                                                                                      >  2:3052256/1‑150 (MQ=255)
                                                          tCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCa                                                                                     >  1:1424793/1‑150 (MQ=255)
                                                               tttCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGt                                                                                <  2:3497997/150‑1 (MQ=255)
                                                               tttCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGt                                                                                >  1:2506232/1‑150 (MQ=255)
                                                               tttCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGt                                                                                >  1:787952/1‑150 (MQ=255)
                                                                    cTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGc                                                                           >  2:4182337/1‑150 (MQ=255)
                                                                     ttCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGcc                                                                          <  1:973190/150‑1 (MQ=255)
                                                                     ttCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGcc                                                                          <  2:1457776/150‑1 (MQ=255)
                                                                        aaaCTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGt                                                                       <  2:1816201/150‑1 (MQ=255)
                                                                          aCTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTaa                                                                     >  2:2293051/1‑150 (MQ=255)
                                                                              cACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGc                                                                 >  2:2511269/1‑150 (MQ=255)
                                                                                 cTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCaaa                                                              >  2:1006545/1‑150 (MQ=255)
                                                                                     gCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGc                                                          >  1:3744877/1‑150 (MQ=255)
                                                                                       cAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGccc                                                        <  2:109008/150‑1 (MQ=255)
                                                                                       cAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGccc                                                        <  2:109010/150‑1 (MQ=255)
                                                                                       cAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGccc                                                        >  1:454269/1‑150 (MQ=255)
                                                                                         gACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGt                                                      >  1:2290950/1‑150 (MQ=255)
                                                                                          aCATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGtt                                                     <  1:2153018/150‑1 (MQ=255)
                                                                                            aTTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGc                                                   >  1:3859052/1‑150 (MQ=255)
                                                                                               cggcggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTgg                                                >  2:2411600/1‑150 (MQ=255)
                                                                                                ggcggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGc                                               >  2:4409201/1‑150 (MQ=255)
                                                                                                  cggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGa                                             >  2:4515019/1‑150 (MQ=255)
                                                                                                   ggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGaa                                            >  1:799816/1‑150 (MQ=255)
                                                                                                   ggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGaa                                            <  1:4081050/150‑1 (MQ=255)
                                                                                                    gTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAAc                                           <  1:2741481/150‑1 (MQ=255)
                                                                                                    gTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAAc                                           >  1:1470003/1‑150 (MQ=255)
                                                                                                    gTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAAc                                           <  1:367132/150‑1 (MQ=255)
                                                                                                      aTCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACgg                                         <  2:4226878/150‑1 (MQ=255)
                                                                                                              tCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCggg                                 <  1:1181317/150‑1 (MQ=255)
                                                                                                               cATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGt                                >  2:14416/1‑150 (MQ=255)
                                                                                                                aTGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTg                               <  1:3073421/150‑1 (MQ=255)
                                                                                                                aTGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTg                               <  1:3932054/150‑1 (MQ=255)
                                                                                                                  gagaTCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGaa                             <  2:2654468/150‑1 (MQ=255)
                                                                                                                   agaTCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGaaa                            <  2:2148389/150‑1 (MQ=255)
                                                                                                                      tCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatca                         <  2:877902/150‑1 (MQ=255)
                                                                                                                       cATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaa                        >  1:2444296/1‑150 (MQ=255)
                                                                                                                         ttttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaatc                      <  2:981373/150‑1 (MQ=255)
                                                                                                                         ttttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaatc                      >  1:1478593/1‑150 (MQ=255)
                                                                                                                          tttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCt                     <  2:494573/150‑1 (MQ=255)
                                                                                                                               gATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAt                >  1:980384/1‑150 (MQ=255)
                                                                                                                                aTTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAtt               >  2:1170858/1‑150 (MQ=255)
                                                                                                                                aTTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAtt               >  2:333208/1‑150 (MQ=255)
                                                                                                                                aTTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAtt               <  1:1611952/150‑1 (MQ=255)
                                                                                                                                 ttGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTc              <  2:1488844/150‑1 (MQ=255)
                                                                                                                                  tgtgAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCg             <  2:4365744/150‑1 (MQ=255)
                                                                                                                                  tgtgAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCg             <  1:2604893/150‑1 (MQ=255)
                                                                                                                                  tgtgAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCg             <  2:3415462/150‑1 (MQ=255)
                                                                                                                                    tgAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGcc           <  1:1007178/150‑1 (MQ=255)
                                                                                                                                     gAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCt          <  1:2920874/150‑1 (MQ=255)
                                                                                                                                      aaaCGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCACACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCtt         >  2:2376891/1‑150 (MQ=255)
                                                                                                                                      aaaCGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCtt         <  1:4546874/150‑1 (MQ=255)
                                                                                                                                      aaaCGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCtt         >  2:755787/1‑150 (MQ=255)
                                                                                                                                      aaaCGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCtt         <  1:4103243/150‑1 (MQ=255)
                                                                                                                                        aCGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCtttt       <  1:638136/150‑1 (MQ=255)
                                                                                                                                         cGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTa      >  1:3546207/1‑150 (MQ=255)
                                                                                                                                         cGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTa      <  2:3574363/150‑1 (MQ=255)
                                                                                                                                           ttAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAAt    >  1:2121476/1‑150 (MQ=255)
                                                                                                                                            tagatagaCGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATg   >  2:1670767/1‑150 (MQ=255)
                                                                                                                                             agatagACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGa  <  1:4232510/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGA  >  CP009273/410883‑411173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑