Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 538,153 A→C 14.3% E270D (GAA→GAC)  glxK → glycerate kinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273538,1530AC14.3% 636.5 / 118.8 271E270D (GAA→GAC) glxKglycerate kinase II
Reads supporting (aligned to +/- strand):  ref base A (115/116);  new base C (19/20);  total (134/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 277 total aligned reads displayed.

GAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCGGTGA  >  CP009273/538004‑538301
                                                                                                                                                     |                                                                                                                                                    
gaaCTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGaa                                                                                                                                                      >  2:4485397/1‑150 (MQ=255)
gaaCTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGaa                                                                                                                                                      <  1:1855066/150‑1 (MQ=255)
  aCTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACAt                                                                                                                                                    <  1:3684419/150‑1 (MQ=255)
  aCTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAAt                                                                                                                                                    >  1:206970/1‑150 (MQ=255)
     tGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATtgt                                                                                                                                                 >  2:3459482/1‑150 (MQ=255)
        aCAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGtt                                                                                                                                              <  1:1137985/150‑1 (MQ=255)
        aCAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGtt                                                                                                                                              <  2:359912/150‑1 (MQ=255)
        aCAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGtt                                                                                                                                              <  2:3025699/150‑1 (MQ=255)
          aGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGa                                                                                                                                            >  1:4134294/1‑150 (MQ=255)
            gggCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAAt                                                                                                                                          >  1:1404083/1‑150 (MQ=255)
               cTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGTGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCg                                                                                                                                       >  1:3097474/1‑150 (MQ=255)
               cTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCg                                                                                                                                       >  1:1076678/1‑150 (MQ=255)
                 gCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGt                                                                                                                                     <  1:4234995/150‑1 (MQ=255)
                   aaaaTTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCa                                                                                                                                   >  2:4032955/1‑150 (MQ=255)
                     aaTTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAAt                                                                                                                                 >  2:1866221/1‑150 (MQ=255)
                       ttACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCt                                                                                                                               >  1:2685021/1‑150 (MQ=255)
                       ttACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCt                                                                                                                               <  2:2519771/150‑1 (MQ=255)
                          cGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTgc                                                                                                                            <  2:1539421/150‑1 (MQ=255)
                              cGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCgcag                                                                                                                        <  2:1427621/150‑1 (MQ=255)
                                 gtTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGTCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCgaaggca                                                                                                                     >  2:2075379/1‑150 (MQ=255)
                                 gtTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCgcaggca                                                                                                                     <  2:3077630/150‑1 (MQ=255)
                                 gtTATACAACAGCAAACTGAAAATAATGTCTGCCATATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATTCGGTCAATCTTGCgcaggca                                                                                                                     >  2:660951/1‑150 (MQ=255)
                                    ttACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCTGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTg                                                                                                                  >  2:1246700/1‑150 (MQ=255)
                                    ttACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTg                                                                                                                  >  1:3847694/1‑150 (MQ=255)
                                     tACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGc                                                                                                                 >  2:1375798/1‑150 (MQ=255)
                                      acaacaGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCa                                                                                                                <  2:3327109/150‑1 (MQ=255)
                                      acaacaGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCa                                                                                                                <  2:1382262/150‑1 (MQ=255)
                                       caacaGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAg                                                                                                               <  2:1643095/150‑1 (MQ=255)
                                        aacaGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAgg                                                                                                              <  2:2840161/150‑1 (MQ=255)
                                             cAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCgca                                                                                                         <  1:328017/150‑1 (MQ=255)
                                              aaaCTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCgcag                                                                                                        <  2:4157619/150‑1 (MQ=255)
                                                  tGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACt                                                                                                    >  1:853148/1‑150 (MQ=255)
                                                      aTTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTtgtg                                                                                                <  1:2793747/150‑1 (MQ=255)
                                                      aTTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTg                                                                                                >  1:2703860/1‑150 (MQ=255)
                                                      aTTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTg                                                                                                <  1:2793875/150‑1 (MQ=255)
                                                        tAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGAt                                                                                              >  1:2989274/1‑150 (MQ=255)
                                                          aTGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTa                                                                                            <  2:2891127/150‑1 (MQ=255)
                                                            gTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTAcc                                                                                          <  2:4539058/150‑1 (MQ=255)
                                                               tGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCggg                                                                                       <  1:3531609/150‑1 (MQ=255)
                                                                gCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCgggg                                                                                      <  1:1037380/150‑1 (MQ=255)
                                                                gCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCgggg                                                                                      >  1:3982561/1‑150 (MQ=255)
                                                                  cAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGaa                                                                                    >  1:4412577/1‑150 (MQ=255)
                                                                   aGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAg                                                                                   >  1:543545/1‑150 (MQ=255)
                                                                   aGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAg                                                                                   <  1:622143/150‑1 (MQ=255)
                                                                    gATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAgg                                                                                  >  1:3059845/1‑150 (MQ=255)
                                                                     aTGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAggg                                                                                 <  1:1801184/150‑1 (MQ=255)
                                                                     aTGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAggg                                                                                 >  1:2562579/1‑150 (MQ=255)
                                                                     aTGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAggg                                                                                 >  1:331267/1‑150 (MQ=255)
                                                                      tGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGTGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGgc                                                                                <  2:1139052/150‑1 (MQ=255)
                                                                       ggcgggcgGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGAGGACCGGGGAAGGgcg                                                                               <  2:4275729/150‑1 (MQ=255)
                                                                          gggcggCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCTCTGGTGATTACCGGGGAAGGGCGCAt                                                                            >  2:2350293/1‑150 (MQ=255)
                                                                            gcggcggcgCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCg                                                                          <  2:2839995/150‑1 (MQ=255)
                                                                              ggcggcgCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGAc                                                                        >  1:2598269/1‑150 (MQ=255)
                                                                              ggcggcgCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGAc                                                                        <  1:3628716/150‑1 (MQ=255)
                                                                              ggcggcgCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGAc                                                                        >  2:3509825/1‑150 (MQ=255)
                                                                              ggcggcgCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGAc                                                                        <  1:4620598/150‑1 (MQ=255)
                                                                              ggcggcgCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGAc                                                                        >  2:3901481/1‑150 (MQ=255)
                                                                                 ggcgCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCg                                                                     <  1:4359589/150‑1 (MQ=255)
                                                                                 ggcgCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCg                                                                     >  1:679688/1‑150 (MQ=255)
                                                                                   cgcTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCa                                                                   <  1:2406588/150‑1 (MQ=255)
                                                                                    gcTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCaa                                                                  <  1:691840/150‑1 (MQ=255)
                                                                                     cTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCaaa                                                                 >  1:863275/1‑150 (MQ=255)
                                                                                         gggcggTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGc                                                             >  1:459561/1‑150 (MQ=255)
                                                                                           gcggTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAg                                                           <  2:3205953/150‑1 (MQ=255)
                                                                                            cggTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAgg                                                          <  1:3249936/150‑1 (MQ=255)
                                                                                             ggTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGc                                                         <  1:91417/150‑1 (MQ=255)
                                                                                                 tGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTa                                                     <  1:232153/150‑1 (MQ=255)
                                                                                                   ggTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTaaa                                                   >  1:1041666/1‑150 (MQ=255)
                                                                                                   ggTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTaaa                                                   <  1:2183002/150‑1 (MQ=255)
                                                                                                     tATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAgc                                                 <  2:3524487/150‑1 (MQ=255)
                                                                                                      aTTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAgcg                                                <  2:4324681/150‑1 (MQ=255)
                                                                                                      aTTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAgcg                                                <  2:1998305/150‑1 (MQ=255)
                                                                                                          cggcggcggTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCACACGGCAGGCGGTAAAGcgccgc                                            >  2:4299529/1‑150 (MQ=255)
                                                                                                           ggcggcggTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCt                                           >  2:333123/1‑150 (MQ=255)
                                                                                                              ggcggTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTggg                                        >  1:756653/1‑150 (MQ=255)
                                                                                                                 ggTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCTACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGgtgt                                     >  1:1626646/1‑150 (MQ=255)
                                                                                                                  gTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGtgtg                                    <  2:918510/150‑1 (MQ=255)
                                                                                                                  gTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGtgtg                                    <  1:2444554/150‑1 (MQ=255)
                                                                                                                  gTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGtgtg                                    >  2:2710212/1‑150 (MQ=255)
                                                                                                                   tATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTgg                                   >  1:1537935/1‑150 (MQ=255)
                                                                                                                   tATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTgg                                   <  1:2474604/150‑1 (MQ=255)
                                                                                                                    aTTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGc                                  <  2:548395/150‑1 (MQ=255)
                                                                                                                     tttCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCg                                 <  1:4316408/150‑1 (MQ=255)
                                                                                                                       tctcAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTc                               <  2:83013/150‑1 (MQ=255)
                                                                                                                       tctcAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTc                               >  1:1749611/1‑150 (MQ=255)
                                                                                                                        ctcAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCg                              <  2:2369641/150‑1 (MQ=255)
                                                                                                                            aTGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTgg                          <  2:3817132/150‑1 (MQ=255)
                                                                                                                                 gATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCCTTGCAGGGCGCAGCAATGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAg                     <  2:1083523/150‑1 (MQ=255)
                                                                                                                                   tatTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTGGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCa                   <  2:1792504/150‑1 (MQ=255)
                                                                                                                                    atTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCCGTGGCGAAGCAg                  <  2:2169919/150‑1 (MQ=255)
                                                                                                                                        aaCCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTa              >  1:4001875/1‑150 (MQ=255)
                                                                                                                                        aaCCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTa              <  1:3257552/150‑1 (MQ=255)
                                                                                                                                         aCCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTaa             >  1:3989434/1‑150 (MQ=255)
                                                                                                                                            gggCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGt          <  1:1386204/150‑1 (MQ=255)
                                                                                                                                              gCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTAc        <  1:4221193/150‑1 (MQ=255)
                                                                                                                                               cATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTAcc       <  2:3295820/150‑1 (MQ=255)
                                                                                                                                                aTTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCg      >  2:2387161/1‑150 (MQ=255)
                                                                                                                                                 ttGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGACGGGCGCATCGACTCGCAAACGGCAGGTGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCgg     >  2:1156471/1‑150 (MQ=255)
                                                                                                                                                  tGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCGGt    <  2:1820442/150‑1 (MQ=255)
                                                                                                                                                   gAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCGGTg   <  2:4629509/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCGGTGA  >  CP009273/538004‑538301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑