Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 557,592 G→T 93.1% D814Y (GAC→TAC)  sfmD → putative outer membrane export usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273557,5920GT93.1% 771.3 / 46.2 257D814Y (GAC→TAC) sfmDputative outer membrane export usher protein
Reads supporting (aligned to +/- strand):  ref base G (8/10);  new base T (96/143);  total (104/153)
Fisher's exact test for biased strand distribution p-value = 8.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

CCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGC  >  CP009273/557445‑557738
                                                                                                                                                   |                                                                                                                                                  
ccGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGAc                                                                                                                                                  >  1:17172/1‑150 (MQ=255)
 cGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACg                                                                                                                                                 <  2:3868111/150‑1 (MQ=255)
 cGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACg                                                                                                                                                 <  2:2524395/150‑1 (MQ=255)
  gACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACgg                                                                                                                                                <  1:2496692/150‑1 (MQ=255)
      cAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCaa                                                                                                                                            >  1:2336584/1‑150 (MQ=255)
        aGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGt                                                                                                                                          >  2:1947676/1‑150 (MQ=255)
         ggCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGtt                                                                                                                                         <  1:3336997/150‑1 (MQ=255)
            gcgTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCTCTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGtttat                                                                                                                                      <  1:4402763/150‑1 (MQ=255)
            gcgGTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCAATACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGAATGGTGGGTGATTACGGGCAAGtttat                                                                                                                                      >  1:1999650/1‑150 (MQ=255)
              gTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGtttattt                                                                                                                                    >  2:3212251/1‑150 (MQ=255)
                tGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTaa                                                                                                                                  >  2:2637146/1‑150 (MQ=255)
                tGGTTCGTGCCAATTTTGATACCCGCATAGGCGGGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTaa                                                                                                                                  >  2:1370672/1‑150 (MQ=255)
                  gTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgt                                                                                                                                <  1:4092950/150‑1 (MQ=255)
                    tCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtgg                                                                                                                              <  2:4273881/150‑1 (MQ=255)
                    tCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtgg                                                                                                                              <  1:4010438/150‑1 (MQ=255)
                       tGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTgc                                                                                                                           <  1:2983716/150‑1 (MQ=255)
                       tGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTgc                                                                                                                           <  1:2984162/150‑1 (MQ=255)
                           aaTTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCa                                                                                                                       >  2:1909385/1‑150 (MQ=255)
                            aTTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCAt                                                                                                                      >  2:221281/1‑150 (MQ=255)
                            aTTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCAt                                                                                                                      <  1:718006/150‑1 (MQ=255)
                            aTTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCAt                                                                                                                      <  1:2270618/150‑1 (MQ=255)
                             ttttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCAtt                                                                                                                     <  1:176889/150‑1 (MQ=255)
                             ttttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCAtt                                                                                                                     <  1:2630588/150‑1 (MQ=255)
                               ttGATACCCGCATAGGCGTGCGGGCGCTAATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGt                                                                                                                   <  2:4252014/150‑1 (MQ=255)
                                   tACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTgg                                                                                                               >  2:2695171/1‑150 (MQ=255)
                                      ccGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGa                                                                                                            <  2:2868538/150‑1 (MQ=255)
                                      ccGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGa                                                                                                            <  1:700277/150‑1 (MQ=255)
                                        gCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAAt                                                                                                          >  1:1649740/1‑150 (MQ=255)
                                        gCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAAt                                                                                                          <  2:1548514/150‑1 (MQ=255)
                                          aTAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTa                                                                                                        <  2:228394/150‑1 (MQ=255)
                                          aTAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTa                                                                                                        <  1:3775352/150‑1 (MQ=255)
                                            aGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACt                                                                                                      >  1:2617153/1‑150 (MQ=255)
                                                gTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGtt                                                                                                  <  1:1174402/150‑1 (MQ=255)
                                                 tGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTc                                                                                                 >  2:1605893/1‑150 (MQ=255)
                                                 tGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTc                                                                                                 <  2:3456571/150‑1 (MQ=255)
                                                    gggCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGt                                                                                              <  2:1062286/150‑1 (MQ=255)
                                                    gggCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGt                                                                                              <  2:4452309/150‑1 (MQ=255)
                                                       cgcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTggg                                                                                           <  1:4469663/150‑1 (MQ=255)
                                                        gcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTgggg                                                                                          <  1:4605025/150‑1 (MQ=255)
                                                        gcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTgggg                                                                                          >  1:4249632/1‑150 (MQ=255)
                                                         cTCATTACCGGTCCCCAGGGCGGAAAACCGGTGCCGTGTGGATCACTGGTACGGGAAAACAGTACCGGCATAACCAGGATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGga                                                                                         <  2:3680084/150‑1 (MQ=255)
                                                            aTTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGAc                                                                                      <  1:1688043/150‑1 (MQ=255)
                                                                ccGTTACCCAGGGCGGAAAAGCGGTGCAGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACTgcg                                                                                  <  2:3257461/150‑1 (MQ=255)
                                                                  gTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGcgcg                                                                                <  2:4011357/150‑1 (MQ=255)
                                                                     aCCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAAc                                                                             <  2:60950/150‑1 (MQ=255)
                                                                        cAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCa                                                                          <  1:3652863/150‑1 (MQ=255)
                                                                        cAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCa                                                                          >  2:1728202/1‑150 (MQ=255)
                                                                         aGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCAc                                                                         <  2:2786010/150‑1 (MQ=255)
                                                                            gCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCt                                                                      <  2:342751/150‑1 (MQ=255)
                                                                              ggAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGc                                                                    >  1:1286343/1‑150 (MQ=255)
                                                                              ggAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGc                                                                    >  1:2407416/1‑150 (MQ=255)
                                                                               gAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCa                                                                   >  2:2158783/1‑150 (MQ=255)
                                                                                        tGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACt                                                          <  1:875588/150‑1 (MQ=255)
                                                                                          ccGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTAt                                                        <  1:101221/150‑1 (MQ=255)
                                                                                            gTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGt                                                      <  1:4243683/150‑1 (MQ=255)
                                                                                              ttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAt                                                    >  2:1058195/1‑150 (MQ=255)
                                                                                              ttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAt                                                    >  2:3373428/1‑150 (MQ=255)
                                                                                              ttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAt                                                    <  1:1558863/150‑1 (MQ=255)
                                                                                              ttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAt                                                    >  2:4530131/1‑150 (MQ=255)
                                                                                               tGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAtt                                                   >  2:3946491/1‑150 (MQ=255)
                                                                                                ggATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTg                                                  >  1:156669/1‑150 (MQ=255)
                                                                                                  aTCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGcc                                                <  1:115581/150‑1 (MQ=255)
                                                                                                  aTCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGcc                                                >  2:2179767/1‑150 (MQ=255)
                                                                                                    cACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGa                                              <  1:3823876/150‑1 (MQ=255)
                                                                                                    cACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGa                                              <  1:331747/150‑1 (MQ=255)
                                                                                                     aCTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGaa                                             >  1:4448655/1‑150 (MQ=255)
                                                                                                      cTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAg                                            <  1:4599198/150‑1 (MQ=255)
                                                                                                       tGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGc                                           >  1:1930370/1‑150 (MQ=255)
                                                                                                        ggTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCa                                          <  2:2490251/150‑1 (MQ=255)
                                                                                                        ggTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCa                                          <  2:2961838/150‑1 (MQ=255)
                                                                                                        ggTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCa                                          <  1:313291/150‑1 (MQ=255)
                                                                                                         gTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCaa                                         >  1:1215182/1‑150 (MQ=255)
                                                                                                          tACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCaaa                                        >  2:1245176/1‑150 (MQ=255)
                                                                                                          tACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCaaa                                        >  2:3140490/1‑150 (MQ=255)
                                                                                                             gggAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCt                                     >  2:222384/1‑150 (MQ=255)
                                                                                                             gggAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCt                                     <  1:3902010/150‑1 (MQ=255)
                                                                                                              ggAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCtt                                    <  1:3081559/150‑1 (MQ=255)
                                                                                                              ggAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCtt                                    >  1:3446651/1‑150 (MQ=255)
                                                                                                              ggAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCtt                                    >  2:3974106/1‑150 (MQ=255)
                                                                                                               gAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTa                                   >  1:2285600/1‑150 (MQ=255)
                                                                                                                    cAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCa                              <  1:2071618/150‑1 (MQ=255)
                                                                                                                    cAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCa                              >  1:4374191/1‑150 (MQ=255)
                                                                                                                      gTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAg                            <  1:1086728/150‑1 (MQ=255)
                                                                                                                        aCCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGcc                          >  1:4149449/1‑150 (MQ=255)
                                                                                                                        aCCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGcc                          >  2:471603/1‑150 (MQ=255)
                                                                                                                          cGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGt                        >  1:2757119/1‑150 (MQ=255)
                                                                                                                          cGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGt                        <  1:2566975/150‑1 (MQ=255)
                                                                                                                           ggAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTc                       <  1:808246/150‑1 (MQ=255)
                                                                                                                           ggAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTc                       <  1:3958359/150‑1 (MQ=255)
                                                                                                                              aTAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACt                    <  2:762859/150‑1 (MQ=255)
                                                                                                                               tAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCACTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTg                   >  1:2930298/1‑150 (MQ=255)
                                                                                                                                 aCCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGtt                 <  1:1756155/150‑1 (MQ=255)
                                                                                                                                  ccAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTa                <  2:858991/150‑1 (MQ=255)
                                                                                                                                       aTGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCg           <  1:2577495/150‑1 (MQ=255)
                                                                                                                                        tggtggGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCgg          >  1:1422871/1‑150 (MQ=255)
                                                                                                                                        tggtggGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCgg          <  2:839548/150‑1 (MQ=255)
                                                                                                                                          gtgggtgATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCa        <  2:4445300/150‑1 (MQ=255)
                                                                                                                                            gggtgATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGt      <  1:3299487/150‑1 (MQ=255)
                                                                                                                                               tgattaCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTg   <  2:3888519/150‑1 (MQ=255)
                                                                                                                                               tgattaCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTg   <  2:3570373/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGC  >  CP009273/557445‑557738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑