Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 632,286 C→A 46.9% E263D (GAG→GAT)  ybdO ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273632,2860CA46.9% 175.7 / inf 264E263D (GAG→GAT) ybdOputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (68/72);  new base A (58/66);  total (126/138)
Fisher's exact test for biased strand distribution p-value = 8.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 275 total aligned reads displayed.

AGATGAAAGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTC  >  CP009273/632137‑632435
                                                                                                                                                     |                                                                                                                                                     
aGATGAAAGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTAtc                                                                                                                                                       >  1:1652655/1‑150 (MQ=255)
 gATGAAAGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTAtct                                                                                                                                                      >  1:3567734/1‑150 (MQ=255)
  aTGAAAGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTAtctc                                                                                                                                                     >  1:85015/1‑150 (MQ=255)
  aTGAAAGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTAtatc                                                                                                                                                     >  1:3779616/1‑150 (MQ=255)
   tGAAAGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATatct                                                                                                                                                    >  1:3536650/1‑150 (MQ=255)
     aaaGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCttt                                                                                                                                                  >  1:677342/1‑150 (MQ=255)
        gTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTttaa                                                                                                                                               >  1:1123452/1‑150 (MQ=255)
           ataGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTttaagtt                                                                                                                                            <  2:2841544/150‑1 (MQ=255)
            tagtagCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTttaagtta                                                                                                                                           <  2:1824179/150‑1 (MQ=255)
             agtagCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTttaagttaa                                                                                                                                          <  1:4015460/150‑1 (MQ=255)
                agCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGaa                                                                                                                                       <  2:2086007/150‑1 (MQ=255)
                agCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGaa                                                                                                                                       <  2:3912242/150‑1 (MQ=255)
                agCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGaa                                                                                                                                       >  1:3376364/1‑150 (MQ=255)
                  ctgtctgtAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAg                                                                                                                                     <  2:3443754/150‑1 (MQ=255)
                    gtctgtAGTTATATCTCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAACGCTTTCTTGAAGGTAACGGATGTTCGAGTTTTATATCATTTAAGTTAAGAAAGTc                                                                                                                                   <  2:3748826/150‑1 (MQ=255)
                    gtctgtAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTc                                                                                                                                   <  1:1798088/150‑1 (MQ=255)
                         tAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGt                                                                                                                              >  1:3419408/1‑150 (MQ=255)
                          aGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGACATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTa                                                                                                                             >  2:2319633/1‑150 (MQ=255)
                          aGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTa                                                                                                                             <  2:452879/150‑1 (MQ=255)
                          aGTTATATATCAAAAAATCTCATTTAGACGGTCAATAAAACGGGAGAACACCAAATTATATAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATATTATAAGAAAAGAAAGTCAccgaa                                                                                                                             >  2:3064696/1‑146 (MQ=255)
                           gTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAg                                                                                                                            <  1:4181866/150‑1 (MQ=255)
                           gTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAg                                                                                                                            >  1:366992/1‑150 (MQ=255)
                              atatatCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGaa                                                                                                                         >  2:530278/1‑150 (MQ=255)
                                atatCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAAtt                                                                                                                       <  1:4203920/150‑1 (MQ=255)
                                   tCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATtata                                                                                                                    <  1:4432179/150‑1 (MQ=255)
                                     aaaaaCTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAATTCACGGTAGGAATTATaaa                                                                                                                  >  1:1218891/1‑150 (MQ=255)
                                       aaaCTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGt                                                                                                                >  1:2253414/1‑150 (MQ=255)
                                       aaaCTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGt                                                                                                                <  1:4332818/150‑1 (MQ=255)
                                        aaCTCTCATTTAGACTGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGtt                                                                                                               <  2:2524401/150‑1 (MQ=255)
                                          ctctCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCa                                                                                                             >  2:920207/1‑150 (MQ=255)
                                          ctctCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCa                                                                                                             >  2:920264/1‑150 (MQ=255)
                                           tctcATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCAt                                                                                                            >  1:4149020/1‑150 (MQ=255)
                                           tctcATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCAt                                                                                                            >  1:2838879/1‑150 (MQ=255)
                                               aTTTAGACGTTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAggg                                                                                                        <  2:1174364/150‑1 (MQ=255)
                                               aTTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAATGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAggg                                                                                                        >  2:3286191/1‑150 (MQ=255)
                                               aTTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAggg                                                                                                        <  1:329718/150‑1 (MQ=255)
                                               aTTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAggg                                                                                                        <  1:813137/150‑1 (MQ=255)
                                               aTTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAggg                                                                                                        <  2:3576115/150‑1 (MQ=255)
                                               aTTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAggg                                                                                                        <  2:1507976/150‑1 (MQ=255)
                                               aTTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAggg                                                                                                        <  2:964606/150‑1 (MQ=255)
                                                tttAGACGGTCAATAAATCGGGAGAACACCACATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAgggg                                                                                                       <  2:2193527/150‑1 (MQ=255)
                                                   aGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACt                                                                                                    <  2:2968658/150‑1 (MQ=255)
                                                   aGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACt                                                                                                    <  2:2270366/150‑1 (MQ=255)
                                                   aGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACt                                                                                                    <  2:1550928/150‑1 (MQ=255)
                                                   aGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACt                                                                                                    >  2:2481830/1‑150 (MQ=255)
                                                      cGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTAtt                                                                                                 <  2:945807/150‑1 (MQ=255)
                                                      cGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTAtt                                                                                                 <  1:2791433/150‑1 (MQ=255)
                                                      cGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTAtt                                                                                                 >  2:4035737/1‑150 (MQ=255)
                                                         tCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTccc                                                                                              <  2:841872/150‑1 (MQ=255)
                                                          cAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCa                                                                                             >  1:823608/1‑150 (MQ=255)
                                                          cAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCa                                                                                             >  2:1695445/1‑150 (MQ=255)
                                                           aaTAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAg                                                                                            <  2:1783303/150‑1 (MQ=255)
                                                           aaTAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAg                                                                                            <  2:3225898/150‑1 (MQ=255)
                                                                aTCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAAt                                                                                       <  2:674041/150‑1 (MQ=255)
                                                                  cGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCt                                                                                     <  1:536521/150‑1 (MQ=255)
                                                                   gggAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTg                                                                                    <  1:634289/150‑1 (MQ=255)
                                                                   gggAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTg                                                                                    <  1:3152051/150‑1 (MQ=255)
                                                                    ggAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGt                                                                                   >  1:4583085/1‑150 (MQ=255)
                                                                    ggAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATATTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGt                                                                                   >  2:1697328/1‑150 (MQ=255)
                                                                       gaACAACAAATTATTTAAAGAAGATTTATTATAGGTAATGTAAAGCTTTATTGAATGTAAAGGATGTTCTTGTTTTATATCTTTTAATTTAAGAAAGTCACGGTAGTAATTATATAGTTCATAGGGGACTATTCCCAGTAAATCTGTAAc                                                                                <  1:2913007/150‑1 (MQ=255)
                                                                        aaCACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACt                                                                               >  1:4215272/1‑150 (MQ=255)
                                                                           acCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCa                                                                            >  2:2509142/1‑150 (MQ=255)
                                                                             cAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAAt                                                                          >  2:428432/1‑150 (MQ=255)
                                                                                attattTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCt                                                                       >  2:3013450/1‑150 (MQ=255)
                                                                                attattTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAAGTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAAATGCAATGCt                                                                       >  2:3886377/1‑150 (MQ=255)
                                                                                attattTAAAGAAGATATATTAAAGGACATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATATGTACCTGCAATGCt                                                                       >  2:3886230/1‑150 (MQ=255)
                                                                                     ttAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTaa                                                                  >  2:1052251/1‑150 (MQ=255)
                                                                                      tAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAAt                                                                 <  2:609767/150‑1 (MQ=255)
                                                                                       aaaGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATg                                                                >  2:625129/1‑150 (MQ=255)
                                                                                        aagaagATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGa                                                               >  1:3911743/1‑150 (MQ=255)
                                                                                        aagaagATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGa                                                               <  2:2819179/150‑1 (MQ=255)
                                                                                          gaagaTTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGAtt                                                             <  1:1312731/150‑1 (MQ=255)
                                                                                           aagaTTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTg                                                            >  2:1416673/1‑150 (MQ=255)
                                                                                              aTTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCa                                                         <  2:3430458/150‑1 (MQ=255)
                                                                                               tttATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAg                                                        >  1:234353/1‑150 (MQ=255)
                                                                                                  attatAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCaa                                                     <  1:1414868/150‑1 (MQ=255)
                                                                                                    tataGGAAATGTAAATCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAag                                                   <  2:3144183/150‑1 (MQ=255)
                                                                                                       aGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAgg                                                >  2:4482372/1‑150 (MQ=255)
                                                                                                        ggAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGa                                               <  2:4148383/150‑1 (MQ=255)
                                                                                                           aaTGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAAcc                                            >  1:962539/1‑150 (MQ=255)
                                                                                                           aaTGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAAcc                                            >  1:46440/1‑150 (MQ=255)
                                                                                                                  aGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAAt                                     <  2:3574777/150‑1 (MQ=255)
                                                                                                                         ttGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAttt                              >  1:1002239/1‑150 (MQ=255)
                                                                                                                         ttGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAttt                              >  2:2646260/1‑150 (MQ=255)
                                                                                                                         ttGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAttt                              <  1:3050212/150‑1 (MQ=255)
                                                                                                                         ttGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAttt                              >  2:1445067/1‑150 (MQ=255)
                                                                                                                          tGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAtttt                             <  2:2883808/150‑1 (MQ=255)
                                                                                                                          tGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAtttt                             <  2:4543744/150‑1 (MQ=255)
                                                                                                                          tGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAtttt                             >  2:4582183/1‑150 (MQ=255)
                                                                                                                          tGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAtttt                             <  2:2767504/150‑1 (MQ=255)
                                                                                                                                 aaCGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTc                      >  2:4126149/1‑150 (MQ=255)
                                                                                                                                 aaCGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTc                      >  1:3233701/1‑150 (MQ=255)
                                                                                                                                 aaCGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTc                      <  1:1904283/150‑1 (MQ=255)
                                                                                                                                   cGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCAt                    >  2:1326441/1‑150 (MQ=255)
                                                                                                                                    ggATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATa                   >  1:1661072/1‑150 (MQ=255)
                                                                                                                                     gATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATaa                  >  2:2039289/1‑150 (MQ=255)
                                                                                                                                        gTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAAtc               >  2:1308453/1‑150 (MQ=255)
                                                                                                                                          tCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAAtctc             <  1:2488979/150‑1 (MQ=255)
                                                                                                                                               ttttATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTc        <  2:3311890/150‑1 (MQ=255)
                                                                                                                                                 ttATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTa      >  2:4064507/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGATGAAAGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTC  >  CP009273/632137‑632435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑