Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 639,231 G→A 100% L65F (CTT→TTT)  rnk ← regulator of nucleoside diphosphate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273639,2310GA97.1% 972.2 / 11.8 281L65F (CTT→TTT) rnkregulator of nucleoside diphosphate kinase
Reads supporting (aligned to +/- strand):  ref base G (6/2);  new base A (141/130);  total (148/133)
Fisher's exact test for biased strand distribution p-value = 2.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 281 total aligned reads displayed.

CGCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCG  >  CP009273/639084‑639376
                                                                                                                                                   |                                                                                                                                                 
cgccCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAAt                                                                                                                                                 >  1:1620822/1‑150 (MQ=255)
cgccCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAAt                                                                                                                                                 <  2:2347761/150‑1 (MQ=255)
cgccCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAAt                                                                                                                                                 <  2:4071478/150‑1 (MQ=255)
   ccGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGc                                                                                                                                              >  1:3996908/1‑150 (MQ=255)
   ccGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGc                                                                                                                                              >  1:3472602/1‑150 (MQ=255)
       aaGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGaa                                                                                                                                          >  2:1894051/1‑150 (MQ=255)
        aGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAt                                                                                                                                         >  2:804493/1‑150 (MQ=255)
          ttCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAttt                                                                                                                                       >  1:4584125/1‑150 (MQ=255)
          ttCCCAGTGAATGGAATCGCCAACGCGCAGCCCCACCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAttt                                                                                                                                       <  2:3007865/150‑1 (MQ=255)
             ccAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAc                                                                                                                                    >  1:4129794/1‑150 (MQ=255)
               aGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAccc                                                                                                                                  >  1:1001113/1‑150 (MQ=255)
               aGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAccc                                                                                                                                  <  1:246618/150‑1 (MQ=255)
                 tGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCgg                                                                                                                                >  1:543101/1‑150 (MQ=255)
                   aaTGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCt                                                                                                                              <  2:3169740/150‑1 (MQ=255)
                    aTGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTg                                                                                                                             >  2:1719227/1‑150 (MQ=255)
                       gAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTc                                                                                                                          <  1:3484507/150‑1 (MQ=255)
                         aTCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCAt                                                                                                                        <  1:2513121/150‑1 (MQ=255)
                          tCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCAtt                                                                                                                       <  1:1033337/150‑1 (MQ=255)
                            gCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGTCTGTTCATTGt                                                                                                                     <  1:2673001/150‑1 (MQ=255)
                            gCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGt                                                                                                                     >  2:2980809/1‑150 (MQ=255)
                            gCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGt                                                                                                                     <  2:2198533/150‑1 (MQ=255)
                             ccAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTc                                                                                                                    <  2:137475/150‑1 (MQ=255)
                               aaCGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTcac                                                                                                                  <  2:1014409/150‑1 (MQ=255)
                                aCGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTcacc                                                                                                                 <  1:3761388/150‑1 (MQ=255)
                                    gcAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCAcgt                                                                                                             >  1:4540531/1‑150 (MQ=255)
                                      aGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCAcgtcg                                                                                                           <  2:2546140/150‑1 (MQ=255)
                                       gCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCAcgtcgt                                                                                                          <  2:2000506/150‑1 (MQ=255)
                                        ccccAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGACTTTAACCCGGCTGTTCATTGTCACCACGTCgtg                                                                                                         >  2:1968635/1‑150 (MQ=255)
                                        ccccAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCgtg                                                                                                         <  2:2686181/150‑1 (MQ=255)
                                        ccccAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCgtg                                                                                                         >  2:4051909/1‑150 (MQ=255)
                                           cagcagTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTgg                                                                                                      >  2:2434734/1‑150 (MQ=255)
                                             gcagTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCa                                                                                                    <  2:3460246/150‑1 (MQ=255)
                                               agTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGGTCATTGTCACCACGTCGGGTGGCAtc                                                                                                  >  1:75927/1‑150 (MQ=255)
                                                gTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtct                                                                                                 <  2:3200415/150‑1 (MQ=255)
                                                gTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtct                                                                                                 <  1:241789/150‑1 (MQ=255)
                                                    ggCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCtcttc                                                                                             <  1:3248067/150‑1 (MQ=255)
                                                      cACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTg                                                                                           <  2:2572496/150‑1 (MQ=255)
                                                           aCCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGaa                                                                                      >  1:1688691/1‑150 (MQ=255)
                                                              ggAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAacac                                                                                   >  1:1132191/1‑150 (MQ=255)
                                                               gAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAAcaca                                                                                  >  1:4268580/1‑150 (MQ=255)
                                                               gAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAAcaca                                                                                  >  2:359922/1‑150 (MQ=255)
                                                                 gCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAtt                                                                                <  1:1519473/150‑1 (MQ=255)
                                                                  ccATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAttt                                                                               >  2:2279272/1‑150 (MQ=255)
                                                                  acATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAttt                                                                               <  1:1392689/149‑1 (MQ=255)
                                                                   cATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTg                                                                              <  2:2728404/150‑1 (MQ=255)
                                                                   cATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTg                                                                              >  2:835138/1‑150 (MQ=255)
                                                                      aaCGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGgc                                                                           >  2:614498/1‑150 (MQ=255)
                                                                          gAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGa                                                                       >  2:4527140/1‑150 (MQ=255)
                                                                            aaGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATc                                                                     >  2:4220277/1‑150 (MQ=255)
                                                                               cTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCaa                                                                  <  1:1812769/150‑1 (MQ=255)
                                                                                tGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAAc                                                                 >  1:797946/1‑150 (MQ=255)
                                                                                tGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAAc                                                                 >  1:3334840/1‑150 (MQ=255)
                                                                                 gAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAAct                                                                <  2:3408173/150‑1 (MQ=255)
                                                                                  aGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAActc                                                               >  1:3860464/1‑150 (MQ=255)
                                                                                          tATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTa                                                       >  1:58049/1‑150 (MQ=255)
                                                                                              ggTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAAcgc                                                   >  2:2770046/1‑150 (MQ=255)
                                                                                               gTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAAcgcg                                                  <  2:1156305/150‑1 (MQ=255)
                                                                                                 cATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCTATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTc                                                <  1:3522230/150‑1 (MQ=255)
                                                                                                 cATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTc                                                <  2:4442417/150‑1 (MQ=255)
                                                                                                 cATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTc                                                <  2:2667252/150‑1 (MQ=255)
                                                                                                  aTTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCg                                               >  1:2768433/1‑150 (MQ=255)
                                                                                                   tttttGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCgg                                              >  1:3483858/1‑150 (MQ=255)
                                                                                                    ttttGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGc                                             <  1:562050/150‑1 (MQ=255)
                                                                                                       tGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGAt                                          >  2:3893853/1‑150 (MQ=255)
                                                                                                       tGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGAt                                          >  1:3745522/1‑150 (MQ=255)
                                                                                                          cGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGTGATTgg                                       <  2:4301321/150‑1 (MQ=255)
                                                                                                          cGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTgg                                       >  2:1220248/1‑150 (MQ=255)
                                                                                                            gATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCa                                     >  2:3295146/1‑150 (MQ=255)
                                                                                                            gATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCa                                     >  2:4282100/1‑150 (MQ=255)
                                                                                                            gATACACCAGAGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCa                                     <  1:4199877/150‑1 (MQ=255)
                                                                                                              tACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCaaa                                   <  2:1749508/150‑1 (MQ=255)
                                                                                                                 acCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCa                                >  1:4396004/1‑150 (MQ=255)
                                                                                                                  ccAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAg                               <  2:3738683/150‑1 (MQ=255)
                                                                                                                    aGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCa                             >  1:1071862/1‑150 (MQ=255)
                                                                                                                    aGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCa                             >  1:2542779/1‑150 (MQ=255)
                                                                                                                     gCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCAt                            <  1:196114/150‑1 (MQ=255)
                                                                                                                      cGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATa                           <  2:4013412/150‑1 (MQ=255)
                                                                                                                       gTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAg                          <  2:1625358/150‑1 (MQ=255)
                                                                                                                       gTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAg                          <  2:397347/150‑1 (MQ=255)
                                                                                                                        tGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAgg                         >  1:1877079/1‑150 (MQ=255)
                                                                                                                        tGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAgg                         <  1:4411340/150‑1 (MQ=255)
                                                                                                                         gcgcACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGc                        >  2:3805514/1‑150 (MQ=255)
                                                                                                                         gcgcACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGc                        <  1:540005/150‑1 (MQ=255)
                                                                                                                          cgcACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCg                       >  1:945970/1‑150 (MQ=255)
                                                                                                                            cacaCGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCggg                     >  2:1563074/1‑150 (MQ=255)
                                                                                                                             acacGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGt                    >  1:3015206/1‑150 (MQ=255)
                                                                                                                               acGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTg                  <  2:1690397/150‑1 (MQ=255)
                                                                                                                                  cACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTc               >  2:2187744/1‑150 (MQ=255)
                                                                                                                                  cACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTc               <  2:563443/150‑1 (MQ=255)
                                                                                                                                  cACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTc               >  2:1258285/1‑150 (MQ=255)
                                                                                                                                      tCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCcagc           >  1:2079728/1‑150 (MQ=255)
                                                                                                                                       cGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCcagca          >  2:818959/1‑150 (MQ=255)
                                                                                                                                        gCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCcagcag         >  2:1464930/1‑150 (MQ=255)
                                                                                                                                        gCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCcagcag         >  2:689522/1‑150 (MQ=255)
                                                                                                                                        gCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCAGAGGCGGGTTGCTCcagcag         >  2:2068751/1‑150 (MQ=255)
                                                                                                                                         ccATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGa        >  2:1394054/1‑150 (MQ=255)
                                                                                                                                         ccATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGa        >  1:855908/1‑150 (MQ=255)
                                                                                                                                           aTCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAat      <  2:638152/150‑1 (MQ=255)
                                                                                                                                              gCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATc   <  2:289897/150‑1 (MQ=255)
                                                                                                                                               cTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCg  <  1:931362/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCG  >  CP009273/639084‑639376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑