Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 647,234 C→T 100% E27K (GAA→AAA)  citC ← [citrate [pro‑3S]‑lyase] ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273647,2340CT96.0% 951.1 / 20.0 273E27K (GAA→AAA) citC[citrate [pro‑3S]‑lyase] ligase
Reads supporting (aligned to +/- strand):  ref base C (1/10);  new base T (98/164);  total (99/174)
Fisher's exact test for biased strand distribution p-value = 6.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 280 total aligned reads displayed.

TCAGCGCCAGTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACT  >  CP009273/647087‑647380
                                                                                                                                                   |                                                                                                                                                   
 cAGCGCCAGTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATg                                                                                                                                                  >  1:3000492/1‑150 (MQ=255)
  aGCGCCAGTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGc                                                                                                                                                 >  2:4161877/1‑150 (MQ=255)
    cgcCAGTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAg                                                                                                                                               >  2:3200721/1‑150 (MQ=255)
    cgcCAGTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAg                                                                                                                                               <  2:2785073/150‑1 (MQ=255)
     gcCAGTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAgg                                                                                                                                              >  1:1582662/1‑150 (MQ=255)
     gcCAGTCCTTCACCGCGGACGGATTCACTGATAGCAAAGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAgg                                                                                                                                              <  1:4033829/150‑1 (MQ=255)
         gTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGAGAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAAtt                                                                                                                                          >  1:3875272/1‑150 (MQ=255)
           ccTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTgg                                                                                                                                        <  2:3784618/150‑1 (MQ=255)
           ccTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTgg                                                                                                                                        <  2:2201145/150‑1 (MQ=255)
              tCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCg                                                                                                                                     >  1:2161560/1‑150 (MQ=255)
                aCCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGAt                                                                                                                                   <  1:1353626/150‑1 (MQ=255)
                     ggacggaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCg                                                                                                                              <  2:4120095/150‑1 (MQ=255)
                      gacggaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTTTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGc                                                                                                                             <  1:2703993/150‑1 (MQ=255)
                         ggaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑t                                                                                                                         >  2:3758116/1‑150 (MQ=255)
                          gagtCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑tt                                                                                                                        <  2:3727463/147‑1 (MQ=255)
                          gaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑tt                                                                                                                        <  2:4169234/150‑1 (MQ=255)
                          gaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑tt                                                                                                                        <  1:3690637/150‑1 (MQ=255)
                            ttCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑tttt                                                                                                                      <  2:1973635/150‑1 (MQ=255)
                             tCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑ttttt                                                                                                                     <  1:3287048/150‑1 (MQ=255)
                             tCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCA‑ttttt                                                                                                                     <  2:4462430/150‑1 (MQ=255)
                              cACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑tttttt                                                                                                                    >  2:3470490/1‑150 (MQ=255)
                              cACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑tttttt                                                                                                                    >  1:4081688/1‑150 (MQ=255)
                              cACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCAATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCA‑tttttt                                                                                                                    >  2:3519984/1‑150 (MQ=255)
                                 tGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTAt                                                                                                                 <  1:741273/150‑1 (MQ=255)
                                   aTAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTAttt                                                                                                               <  2:1008491/150‑1 (MQ=255)
                                       cAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTg                                                                                                           >  2:1820748/1‑150 (MQ=255)
                                       cAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTg                                                                                                           <  2:1242728/150‑1 (MQ=255)
                                        aaCGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGa                                                                                                          <  2:1061893/150‑1 (MQ=255)
                                          cGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAAc                                                                                                        <  2:1944481/150‑1 (MQ=255)
                                          cGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAAc                                                                                                        <  1:2873142/150‑1 (MQ=255)
                                           gCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACg                                                                                                       <  1:3025573/150‑1 (MQ=255)
                                           gCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACg                                                                                                       >  2:132501/1‑150 (MQ=255)
                                            cATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGt                                                                                                      >  1:4553952/1‑150 (MQ=255)
                                             aTTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGtt                                                                                                     <  2:1361508/150‑1 (MQ=255)
                                             aTTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGtt                                                                                                     <  2:2643913/150‑1 (MQ=255)
                                             aTTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGtt                                                                                                     <  1:3612715/150‑1 (MQ=255)
                                              tttAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGttt                                                                                                    <  1:292275/150‑1 (MQ=255)
                                              tttAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGttt                                                                                                    <  1:1739151/150‑1 (MQ=255)
                                               ttAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGtttt                                                                                                   >  1:2502901/1‑150 (MQ=255)
                                               ttAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGtttt                                                                                                   <  2:1023914/150‑1 (MQ=255)
                                                taataaTATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTa                                                                                                  >  1:4149958/1‑150 (MQ=255)
                                                        tttCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTg                                                                                          <  1:3198772/150‑1 (MQ=255)
                                                        tttCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTg                                                                                          <  2:1670382/150‑1 (MQ=255)
                                                        tttCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTg                                                                                          >  2:1387698/1‑150 (MQ=255)
                                                         ttCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGa                                                                                         <  2:2398719/150‑1 (MQ=255)
                                                          tCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGaa                                                                                        >  2:4322500/1‑150 (MQ=255)
                                                           ccGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGaaa                                                                                       <  1:2237213/150‑1 (MQ=255)
                                                             ggCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAat                                                                                     >  1:679387/1‑150 (MQ=255)
                                                              gCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAata                                                                                    <  2:3314701/150‑1 (MQ=255)
                                                              gCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAata                                                                                    <  1:3681887/150‑1 (MQ=255)
                                                               cAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAatat                                                                                   >  1:2512048/1‑150 (MQ=255)
                                                                aaTTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATc                                                                                  >  1:4338843/1‑150 (MQ=255)
                                                                    ccACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTg                                                                              <  2:3049378/150‑1 (MQ=255)
                                                                     cACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGc                                                                             >  2:4460307/1‑150 (MQ=255)
                                                                      aCCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGcc                                                                            <  2:2934410/150‑1 (MQ=255)
                                                                        cGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGa                                                                          >  2:2559728/1‑150 (MQ=255)
                                                                         gCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGaa                                                                         >  1:1117881/1‑150 (MQ=255)
                                                                          cACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAAc                                                                        <  2:2735245/150‑1 (MQ=255)
                                                                           aCGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACa                                                                       >  1:3355893/1‑150 (MQ=255)
                                                                            cgcgATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACat                                                                      <  2:3620036/150‑1 (MQ=255)
                                                                               gATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACataat                                                                   <  2:3739551/150‑1 (MQ=255)
                                                                                 tAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTTTTTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACataata                                                                  >  2:4527540/1‑150 (MQ=255)
                                                                                  aaGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATaataaa                                                                >  2:67381/1‑150 (MQ=255)
                                                                                  aaGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATaataaa                                                                <  1:125527/150‑1 (MQ=255)
                                                                                   aGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATaataaat                                                               >  2:4196060/1‑150 (MQ=255)
                                                                                    gCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATaataaata                                                              <  1:632054/150‑1 (MQ=255)
                                                                                      tttcCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGt                                                            <  2:2279924/150‑1 (MQ=255)
                                                                                        ttCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTAt                                                          <  2:1478161/150‑1 (MQ=255)
                                                                                          cATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATcc                                                        <  2:932612/150‑1 (MQ=255)
                                                                                           aTCTCGGGTTACTGTAATAAATACTTCGACTGTGTTGTAAACGCTCAAATCATTTTTATGCTGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCt                                                       <  2:451159/150‑1 (MQ=255)
                                                                                           aTCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCt                                                       <  1:1186199/150‑1 (MQ=255)
                                                                                              gcgGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGAGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGaa                                                    <  2:1201989/150‑1 (MQ=255)
                                                                                               cgGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAg                                                   <  2:51106/150‑1 (MQ=255)
                                                                                                  gTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTg                                                >  2:3862047/1‑150 (MQ=255)
                                                                                                    tACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCa                                              <  1:2015444/150‑1 (MQ=255)
                                                                                                          aataaataCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgttgt                                        <  1:1514867/150‑1 (MQ=255)
                                                                                                           ataaataCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATgttgtt                                       <  2:3819523/150‑1 (MQ=255)
                                                                                                                 aCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAt                                 <  2:2659347/150‑1 (MQ=255)
                                                                                                                 aCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAt                                 <  1:2309339/150‑1 (MQ=255)
                                                                                                                    tCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTg                              <  2:3025862/150‑1 (MQ=255)
                                                                                                                      gACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCa                            >  1:1869162/1‑150 (MQ=255)
                                                                                                                         tgtgGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACg                         <  2:4074274/150‑1 (MQ=255)
                                                                                                                            ggtgTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAt                      <  1:1021955/150‑1 (MQ=255)
                                                                                                                             gtgtCAACGCTCAAATCATTTTTATGCAGGATTTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGTACGTTTTACGCGGGTGAAAATATCATTGCCGAAAATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAtg                     <  1:3798310/150‑1 (MQ=255)
                                                                                                                             gtgtCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAtg                     >  1:2288039/1‑150 (MQ=255)
                                                                                                                                tCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGTTGCATGTTGTTATCGATTTGCAACGAAtgttg                  <  1:842907/150‑1 (MQ=255)
                                                                                                                                  aaCGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATgttgtt                <  1:4165120/150‑1 (MQ=255)
                                                                                                                                    cGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCa              <  1:342511/150‑1 (MQ=255)
                                                                                                                                    cGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCa              <  1:3935936/150‑1 (MQ=255)
                                                                                                                                    cGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCa              <  1:4496739/150‑1 (MQ=255)
                                                                                                                                       tCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATg           >  2:1834730/1‑150 (MQ=255)
                                                                                                                                        cAACTCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGt          <  1:1647238/150‑1 (MQ=255)
                                                                                                                                        cAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGt          >  2:2075104/1‑150 (MQ=255)
                                                                                                                                          aaTCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTg        <  2:2679204/150‑1 (MQ=255)
                                                                                                                                           aTCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGc       >  2:623583/1‑150 (MQ=255)
                                                                                                                                            tCATTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCa      <  1:4416134/150‑1 (MQ=255)
                                                                                                                                              aTTTTTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCaaa    <  2:2177932/150‑1 (MQ=255)
                                                                                                                                               ttttTATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAAc   <  1:978322/150‑1 (MQ=255)
                                                                                                                                                ttttATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACt  <  2:926974/150‑1 (MQ=255)
                                                                                                                                                ttttATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACt  <  2:927024/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCAGCGCCAGTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCA‑TTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACT  >  CP009273/647087‑647380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑