Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 662,631 C→A 100% R348L (CGC→CTC)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273662,6310CA95.6% 961.8 / 29.8 298R348L (CGC→CTC) mrdAtranspeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand):  ref base C (6/7);  new base A (158/126);  total (165/133)
Fisher's exact test for biased strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 307 total aligned reads displayed.

TAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACC  >  CP009273/662484‑662776
                                                                                                                                                   |                                                                                                                                                 
tAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTgagc                                                                                                                                                 >  1:3854926/1‑150 (MQ=255)
tAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTgagc                                                                                                                                                 <  1:2745343/150‑1 (MQ=255)
tAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTgagc                                                                                                                                                 >  1:4467432/1‑150 (MQ=255)
 aGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGagcg                                                                                                                                                <  1:2678529/150‑1 (MQ=255)
   gCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGAGAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTg                                                                                                                                              <  2:2420359/150‑1 (MQ=255)
    ccACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGa                                                                                                                                             >  2:2601852/1‑150 (MQ=255)
     cACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGAt                                                                                                                                            >  1:859/1‑150 (MQ=255)
      aCCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATc                                                                                                                                           <  2:4437796/150‑1 (MQ=255)
          gATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCAccc                                                                                                                                       >  2:3200538/1‑150 (MQ=255)
           aTAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCAcccc                                                                                                                                      <  2:3858719/150‑1 (MQ=255)
           aTAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCAcccc                                                                                                                                      >  2:1442019/1‑150 (MQ=255)
               aagaatGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTTGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGgcg                                                                                                                                  <  2:637609/150‑1 (MQ=255)
               aagaagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGgcg                                                                                                                                  <  1:509109/150‑1 (MQ=255)
                agaagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGgcgc                                                                                                                                 >  2:2352528/1‑150 (MQ=255)
                 gaagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCt                                                                                                                                <  1:2871379/150‑1 (MQ=255)
                 gaagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCt                                                                                                                                >  2:3454417/1‑150 (MQ=255)
                  aagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTc                                                                                                                               >  2:2159239/1‑150 (MQ=255)
                  aagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTc                                                                                                                               >  2:2898723/1‑150 (MQ=255)
                   agGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCa                                                                                                                              >  1:2429798/1‑150 (MQ=255)
                          cGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGa                                                                                                                       <  1:1113391/150‑1 (MQ=255)
                              gATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAAcc                                                                                                                   <  2:3889580/150‑1 (MQ=255)
                                ttcttcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGc                                                                                                                 >  2:2833474/1‑150 (MQ=255)
                                ttcttcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGc                                                                                                                 >  2:1567196/1‑150 (MQ=255)
                                  cttcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCca                                                                                                               >  1:1518631/1‑150 (MQ=255)
                                   ttcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCcac                                                                                                              >  2:2659686/1‑150 (MQ=255)
                                   ttcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCcac                                                                                                              >  2:542490/1‑150 (MQ=255)
                                    tcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCcaca                                                                                                             >  2:73263/1‑150 (MQ=255)
                                    tcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCcaca                                                                                                             <  1:2766520/150‑1 (MQ=255)
                                     ccAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACAt                                                                                                            >  1:2992638/1‑150 (MQ=255)
                                          gATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGt                                                                                                       >  1:3278619/1‑150 (MQ=255)
                                               tgtgACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAAc                                                                                                  >  2:4614632/1‑150 (MQ=255)
                                               tgtgACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAAc                                                                                                  <  2:3714992/150‑1 (MQ=255)
                                                gtgACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACt                                                                                                 >  2:527523/1‑150 (MQ=255)
                                                   aCATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTa                                                                                              <  1:2628404/150‑1 (MQ=255)
                                                     aTTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGa                                                                                            >  2:4167190/1‑150 (MQ=255)
                                                       tCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAcg                                                                                          >  2:2803115/1‑150 (MQ=255)
                                                          gACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATATGGTTTAACTGTAGACGCgg                                                                                       >  1:4532040/1‑150 (MQ=255)
                                                          gACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCgg                                                                                       <  1:422902/150‑1 (MQ=255)
                                                           aCGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCggg                                                                                      <  2:4218906/150‑1 (MQ=255)
                                                             gCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGggag                                                                                    <  2:4531184/150‑1 (MQ=255)
                                                                cGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGAt                                                                                 >  1:3663839/1‑150 (MQ=255)
                                                                 gTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATa                                                                                >  1:991905/1‑150 (MQ=255)
                                                                  tGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATaa                                                                               >  2:823853/1‑150 (MQ=255)
                                                                     ccccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAcc                                                                            <  2:1644296/150‑1 (MQ=255)
                                                                      cccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAccc                                                                           <  2:3479779/150‑1 (MQ=255)
                                                                      cccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAccc                                                                           <  1:2539903/150‑1 (MQ=255)
                                                                      cccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAccc                                                                           >  1:3079184/1‑150 (MQ=255)
                                                                        cATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACAGTAGACGCGGGAGGATAAAccccc                                                                         >  1:1558765/1‑150 (MQ=255)
                                                                         aTTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCt                                                                        <  1:2486863/150‑1 (MQ=255)
                                                                         aTTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTAGTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCt                                                                        >  1:2017647/1‑150 (MQ=255)
                                                                          ttttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtg                                                                       <  1:1830274/150‑1 (MQ=255)
                                                                            ttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtg                                                                     >  2:2408796/1‑150 (MQ=255)
                                                                             tttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtgt                                                                    >  1:3920462/1‑150 (MQ=255)
                                                                             tttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtgt                                                                    <  2:2535661/150‑1 (MQ=255)
                                                                              ttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtgtg                                                                   <  2:802872/150‑1 (MQ=255)
                                                                              ttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtgtg                                                                   <  1:1399253/150‑1 (MQ=255)
                                                                                ccAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGgc                                                                 >  1:4402330/1‑150 (MQ=255)
                                                                                 cAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGgcg                                                                >  1:843564/1‑150 (MQ=255)
                                                                                  aGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGgcgc                                                               >  2:2020884/1‑150 (MQ=255)
                                                                                  aGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGgcgc                                                               >  2:2981032/1‑150 (MQ=255)
                                                                                  aGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGgcgc                                                               >  1:351767/1‑150 (MQ=255)
                                                                                     cACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGt                                                            >  2:2223994/1‑150 (MQ=255)
                                                                                     cACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGt                                                            >  2:1177430/1‑150 (MQ=255)
                                                                                      aCGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGtt                                                           <  2:2757192/150‑1 (MQ=255)
                                                                                       cGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTc                                                          >  1:1209538/1‑150 (MQ=255)
                                                                                         aTAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCAc                                                        <  1:1416241/150‑1 (MQ=255)
                                                                                           aaCGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCa                                                      <  2:2440477/150‑1 (MQ=255)
                                                                                            aCGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAg                                                     <  2:1932169/150‑1 (MQ=255)
                                                                                            aCGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAg                                                     <  2:2024995/150‑1 (MQ=255)
                                                                                             cGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGc                                                    >  1:643/1‑150 (MQ=255)
                                                                                                    ccGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTAt                                             >  2:4015719/1‑150 (MQ=255)
                                                                                                     cGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTAtt                                            >  2:2472099/1‑150 (MQ=255)
                                                                                                       aaCCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCg                                          >  1:1280408/1‑150 (MQ=255)
                                                                                                          cTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGAt                                       <  2:3248150/150‑1 (MQ=255)
                                                                                                          cTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGAt                                       <  1:7719/150‑1 (MQ=255)
                                                                                                           tGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATc                                      <  2:4370366/150‑1 (MQ=255)
                                                                                                             gCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGt                                    >  1:2288815/1‑150 (MQ=255)
                                                                                                               aGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTc                                  >  2:1782315/1‑150 (MQ=255)
                                                                                                                gTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTca                                 >  2:383172/1‑150 (MQ=255)
                                                                                                                        caGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCg                         >  2:2295099/1‑150 (MQ=255)
                                                                                                                            cTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGaat                     >  2:3645914/1‑150 (MQ=255)
                                                                                                                             tGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGaata                    >  2:1077777/1‑150 (MQ=255)
                                                                                                                              gggTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGaataa                   <  1:3163591/150‑1 (MQ=255)
                                                                                                                               ggTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGaataat                  <  2:3663968/150‑1 (MQ=255)
                                                                                                                               ggTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGaataat                  <  1:1943355/150‑1 (MQ=255)
                                                                                                                               ggTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGaataat                  >  1:4249638/1‑150 (MQ=255)
                                                                                                                                 tCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCt                >  2:3987664/1‑150 (MQ=255)
                                                                                                                                  cAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCtt               >  2:3340082/1‑150 (MQ=255)
                                                                                                                                  cAAACAGCGTCGTATTGAGAGTGATCACCCCGGCGCTCAATGCCGAAACCTCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCtt               <  1:1725637/150‑1 (MQ=255)
                                                                                                                                   aaaCAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCttt              <  1:3594048/150‑1 (MQ=255)
                                                                                                                                    aaCAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTg             <  2:3446324/150‑1 (MQ=255)
                                                                                                                                     aCAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGc            <  1:3038794/150‑1 (MQ=255)
                                                                                                                                      cAGCGTCGTATTGAGCGTGATCACCCCTGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCt           <  2:3587873/150‑1 (MQ=255)
                                                                                                                                       aGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTg          <  1:1016932/150‑1 (MQ=255)
                                                                                                                                         cgtcgtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGga        >  1:561474/1‑150 (MQ=255)
                                                                                                                                         cgtcgtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGga        >  1:3744056/1‑150 (MQ=255)
                                                                                                                                         cgtcgtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGga        >  1:1624381/1‑150 (MQ=255)
                                                                                                                                             gtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGTGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATa    <  2:2311165/150‑1 (MQ=255)
                                                                                                                                               aTTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAcc  <  2:1098523/150‑1 (MQ=255)
                                                                                                                                               aTTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAcc  <  1:2217680/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACC  >  CP009273/662484‑662776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑