Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 667,348 G→T 100% Q99K (CAG→AAG)  lptE ← LPS assembly OM complex LptDE, lipoprotein component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273667,3480GT95.1% 828.8 / 32.4 263Q99K (CAG→AAG) lptELPS assembly OM complex LptDE, lipoprotein component
Reads supporting (aligned to +/- strand):  ref base G (7/6);  new base T (139/111);  total (146/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 264 total aligned reads displayed.

ACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCG  >  CP009273/667201‑667494
                                                                                                                                                   |                                                                                                                                                  
    ctctTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATActc                                                                                                                                              >  1:3283245/1‑150 (MQ=255)
      ctTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctg                                                                                                                                            >  1:3880642/1‑150 (MQ=255)
       ttttACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctgc                                                                                                                                           >  2:1841343/1‑150 (MQ=255)
         ttACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctgctg                                                                                                                                         <  2:2554098/150‑1 (MQ=255)
         ttACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctgctg                                                                                                                                         <  1:1548534/150‑1 (MQ=255)
          tACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGCACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGt                                                                                                                                        <  1:2064769/150‑1 (MQ=255)
           aCGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGtt                                                                                                                                       <  1:575223/150‑1 (MQ=255)
            cGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATGTGCGGGTTATCGACGAACGAACGGAAGACTTTGGCGCTACTCGGGTAGTTATCACGGCCGGGGAGCAACACGGTCGCAGTAACCGTCATGATCATCTTATACGCTGCTGtgt                                                                                                                                      >  1:3928053/1‑148 (MQ=255)
              aTCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGa                                                                                                                                    <  1:2188062/150‑1 (MQ=255)
               tCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGAc                                                                                                                                   >  1:1762222/1‑150 (MQ=255)
               tCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGAc                                                                                                                                   >  2:459359/1‑150 (MQ=255)
               tCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGAc                                                                                                                                   <  1:2411927/150‑1 (MQ=255)
                     cTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTAc                                                                                                                             <  2:900596/150‑1 (MQ=255)
                      ttGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACg                                                                                                                            <  2:298547/150‑1 (MQ=255)
                        gTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGa                                                                                                                          >  1:2007811/1‑150 (MQ=255)
                        gTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGa                                                                                                                          <  2:715901/150‑1 (MQ=255)
                          tCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAAt                                                                                                                        <  2:218711/150‑1 (MQ=255)
                            gTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCATGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATAc                                                                                                                      >  2:1332804/1‑150 (MQ=255)
                            gTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATAc                                                                                                                      >  1:462798/1‑150 (MQ=255)
                              tATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCg                                                                                                                    <  2:283366/150‑1 (MQ=255)
                                tCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGAt                                                                                                                  >  2:3235958/1‑150 (MQ=255)
                                 cTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATg                                                                                                                 <  1:3942388/150‑1 (MQ=255)
                                   ttCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCg                                                                                                               >  2:2329172/1‑150 (MQ=255)
                                   ttCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCg                                                                                                               <  2:4246796/150‑1 (MQ=255)
                                    tCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGTCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCgg                                                                                                              >  2:1439294/1‑150 (MQ=255)
                                     cGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGt                                                                                                             <  2:1748649/150‑1 (MQ=255)
                                       cTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGCTCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTAt                                                                                                           >  2:3874300/1‑150 (MQ=255)
                                       cTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTAt                                                                                                           <  2:3326660/150‑1 (MQ=255)
                                        tAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATc                                                                                                          >  2:2679407/1‑150 (MQ=255)
                                        tAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATc                                                                                                          >  1:1380895/1‑150 (MQ=255)
                                          ccGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCtt                                                                                                        <  2:838676/149‑1 (MQ=255)
                                            gCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTc                                                                                                      <  2:1911334/150‑1 (MQ=255)
                                            gCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTc                                                                                                      <  1:1027162/150‑1 (MQ=255)
                                              cATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTcgc                                                                                                    >  2:865148/1‑150 (MQ=255)
                                               aTTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTcgct                                                                                                   <  2:4029411/150‑2 (MQ=255)
                                                tttGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGa                                                                                                  >  2:3446402/1‑150 (MQ=255)
                                                 ttGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTAt                                                                                                 <  1:985572/150‑1 (MQ=255)
                                                    cGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCt                                                                                              <  2:558153/150‑1 (MQ=255)
                                                    cGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCt                                                                                              >  2:3982335/1‑150 (MQ=255)
                                                    cGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCt                                                                                              <  2:1685443/150‑1 (MQ=255)
                                                    cGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTGTCGCTATGCt                                                                                              >  1:4310806/1‑150 (MQ=255)
                                                     gggTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTc                                                                                             <  1:1007975/150‑1 (MQ=255)
                                                      ggTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCa                                                                                            <  1:1058463/150‑1 (MQ=255)
                                                         tATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACta                                                                                         >  2:2306001/1‑149 (MQ=255)
                                                          aTCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACttt                                                                                        <  1:884134/150‑1 (MQ=255)
                                                           tCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTa                                                                                       >  2:2541148/1‑150 (MQ=255)
                                                            cGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTAc                                                                                      <  1:363966/150‑1 (MQ=255)
                                                            cGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAc                                                                                      >  2:3385842/1‑150 (MQ=255)
                                                             gaagaaCGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAcc                                                                                     >  2:4477683/1‑150 (MQ=255)
                                                              aagaaCGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAccc                                                                                    >  1:2837648/1‑150 (MQ=255)
                                                              aagaaCGAACGGAAGACTGTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGCCCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAccc                                                                                    <  2:4405720/150‑1 (MQ=255)
                                                                gaacgaacGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGACTACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCaa                                                                                  >  1:3068935/1‑150 (MQ=255)
                                                                       cgGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAg                                                                           >  2:1627366/1‑150 (MQ=255)
                                                                          aaGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGAt                                                                        <  2:2470998/150‑1 (MQ=255)
                                                                            gACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATgg                                                                      >  2:1258299/1‑150 (MQ=255)
                                                                               tttGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAAc                                                                   <  2:2991235/150‑1 (MQ=255)
                                                                               tttGGCGCTAATCGGGTAGATAGCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAAc                                                                   <  1:2382553/150‑1 (MQ=255)
                                                                                ttGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACg                                                                  >  2:1077170/1‑150 (MQ=255)
                                                                                 tGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGt                                                                 >  2:2607840/1‑150 (MQ=255)
                                                                                    cgcTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCt                                                              >  2:3209261/1‑150 (MQ=255)
                                                                                     gcTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCtt                                                             <  2:4434849/150‑1 (MQ=255)
                                                                                     gcTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCtt                                                             <  1:2701289/150‑1 (MQ=255)
                                                                                       tAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAc                                                           >  1:4188130/1‑150 (MQ=255)
                                                                                       tAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAc                                                           >  2:3371781/1‑150 (MQ=255)
                                                                                        aaTCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAcg                                                          >  1:535309/1‑150 (MQ=255)
                                                                                        aaTCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAcg                                                          <  2:2854928/150‑1 (MQ=255)
                                                                                        aaTCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAcg                                                          >  1:2847953/1‑150 (MQ=255)
                                                                                           cGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGt                                                       >  1:1920629/1‑150 (MQ=255)
                                                                                            gggTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTg                                                      <  1:4037284/150‑1 (MQ=255)
                                                                                            gggTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTg                                                      >  2:1780041/1‑150 (MQ=255)
                                                                                             ggTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTgg                                                     >  1:119786/1‑150 (MQ=255)
                                                                                             ggTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTgg                                                     <  1:2239030/150‑1 (MQ=255)
                                                                                               tAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGtt                                                   <  1:1582636/150‑1 (MQ=255)
                                                                                               tAGATATCACGGCCGGGGATCAACAAGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGtt                                                   <  1:2573791/150‑1 (MQ=255)
                                                                                                  atatCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCt                                                <  1:3739816/150‑1 (MQ=255)
                                                                                                        cGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCa                                          >  1:1703447/1‑150 (MQ=255)
                                                                                                          gCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAg                                        <  2:4345196/150‑1 (MQ=255)
                                                                                                             ggggATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCaa                                     >  1:1254293/1‑150 (MQ=255)
                                                                                                             ggggATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCaa                                     <  1:2130235/150‑1 (MQ=255)
                                                                                                               ggATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACt                                   <  1:997134/150‑1 (MQ=255)
                                                                                                               ggATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTCTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACt                                   <  1:437256/150‑1 (MQ=255)
                                                                                                                 aTCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCg                                 >  1:4467340/1‑150 (MQ=255)
                                                                                                                    aaCACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGaca                              <  1:144389/150‑1 (MQ=255)
                                                                                                                    aaCACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGaca                              <  2:821082/150‑1 (MQ=255)
                                                                                                                      cacGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACAcc                            >  2:792016/1‑150 (MQ=255)
                                                                                                                         ggTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCAtt                         >  2:3440626/1‑150 (MQ=255)
                                                                                                                           tCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCa                       <  2:220259/150‑1 (MQ=255)
                                                                                                                              cATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGAc                    <  2:4558559/150‑1 (MQ=255)
                                                                                                                               aTTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACg                   <  2:4190920/150‑1 (MQ=255)
                                                                                                                               aTTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACg                   >  1:334981/1‑150 (MQ=255)
                                                                                                                                 tAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTa                 >  2:1327365/1‑150 (MQ=255)
                                                                                                                                 tAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTa                 >  1:3162168/1‑150 (MQ=255)
                                                                                                                                 tAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTa                 >  1:3162390/1‑150 (MQ=255)
                                                                                                                                  aaCCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTaa                >  1:787759/1‑150 (MQ=255)
                                                                                                                                   aCCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAAc               >  2:1866963/1‑150 (MQ=255)
                                                                                                                                    ccGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACt              <  1:4413945/150‑1 (MQ=255)
                                                                                                                                        cATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGtt          >  1:4518680/1‑150 (MQ=255)
                                                                                                                                           gATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcg       >  2:3660427/1‑150 (MQ=255)
                                                                                                                                             tcatcTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcgca     >  1:1073088/1‑150 (MQ=255)
                                                                                                                                              catcTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTATCGCTATGCTCACTTTAACCAAACTCAAGGATGGAACGTCCTTACGCGAGGTTTCTTTATCACGCAACTCGACACCATTCAGACGTAACTGGTTAcgcac    >  2:2042815/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCG  >  CP009273/667201‑667494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑