BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	693,739	G→T	89.0%	H299N (CAC→AAC)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	693,739	0	G	T	89.0%	694.7 / 86.5	262	H299N (CAC→AAC)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand): ref base G (7/22); new base T (114/118); total (122/140)
Fisher's exact test for biased strand distribution p-value = 1.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 279 total aligned reads displayed.

CGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGT  >  CP009273/693592‑693885

cGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAgtgaagttaa                                                                                                                                                  >  2:78006/1‑150 (MQ=255)

 ggACAGCACCATTTTAATGCCCATCGCCTTNATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAt                                                                                                                                                 >  1:4281493/1‑150 (MQ=255)

 ggACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAt                                                                                                                                                 >  1:39399/1‑150 (MQ=255)

  gACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAtt                                                                                                                                                <  1:2019853/150‑1 (MQ=255)

   aCAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAttt                                                                                                                                               <  1:2382699/150‑1 (MQ=255)

     aGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCg                                                                                                                                             >  2:1267823/1‑150 (MQ=255)

     aGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCg                                                                                                                                             >  1:2555640/1‑150 (MQ=255)

     aGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCg                                                                                                                                             <  1:2291535/150‑1 (MQ=255)

       cACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTg                                                                                                                                           >  2:462686/1‑150 (MQ=255)

       cACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTg                                                                                                                                           >  1:3000639/1‑150 (MQ=255)

               tAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCg                                                                                                                                   >  1:474033/1‑150 (MQ=255)

               tAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCg                                                                                                                                   >  2:3985978/1‑150 (MQ=255)

               tAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCg                                                                                                                                   >  1:2151644/1‑150 (MQ=255)

                 aTGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTg                                                                                                                                 <  2:2203202/150‑1 (MQ=255)

                   gCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCTTGTTGAAACGTGcc                                                                                                                               >  1:3273068/1‑150 (MQ=255)

                   gCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGcc                                                                                                                               <  1:439085/150‑1 (MQ=255)

                   gCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGcc                                                                                                                               <  1:555832/150‑1 (MQ=255)

                    cccATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGccc                                                                                                                              <  2:2737568/150‑1 (MQ=255)

                     ccATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCa                                                                                                                             >  1:1332168/1‑150 (MQ=255)

                        tCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGAt                                                                                                                          >  2:1667982/1‑150 (MQ=255)

                         cGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATg                                                                                                                         <  1:981541/150‑1 (MQ=255)

                          gCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATgg                                                                                                                        >  1:194208/1‑150 (MQ=255)

                          gCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATgg                                                                                                                        >  1:2915360/1‑150 (MQ=255)

                             ttGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGttt                                                                                                                     <  2:4354898/150‑1 (MQ=255)

                             ttGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGttt                                                                                                                     <  1:3672923/150‑1 (MQ=255)

                              tGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTg                                                                                                                    >  2:3541254/1‑150 (MQ=255)

                                aTCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGcc                                                                                                                  <  1:4364388/150‑1 (MQ=255)

                                  cTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCAc                                                                                                                >  1:4393155/1‑150 (MQ=255)

                                    tACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACtt                                                                                                              >  1:3412813/1‑150 (MQ=255)

                                    tACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACtt                                                                                                              <  2:1449791/150‑1 (MQ=255)

                                      cgcgACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTcc                                                                                                            <  1:1961396/150‑1 (MQ=255)

                                       gcgACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCt                                                                                                           <  1:3656022/150‑1 (MQ=255)

                                       gcgACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCt                                                                                                           <  1:2624146/150‑1 (MQ=255)

                                        cgACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTg                                                                                                          >  1:1002095/1‑150 (MQ=255)

                                        cgACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTg                                                                                                          <  1:4164436/150‑1 (MQ=255)

                                          aCATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTggg                                                                                                        <  1:1062355/150‑1 (MQ=255)

                                           cATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgc                                                                                                       >  1:602558/1‑150 (MQ=255)

                                               aaaTACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgctgct                                                                                                   <  2:3303129/150‑1 (MQ=255)

                                                aaTACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCtt                                                                                                  >  2:4575515/1‑150 (MQ=255)

                                                  tACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTc                                                                                                >  1:901685/1‑150 (MQ=255)

                                                   aCATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCa                                                                                               >  2:3691661/1‑150 (MQ=255)

                                                   aCATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGACATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCa                                                                                               <  1:4229930/150‑1 (MQ=255)

                                                      tCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGAt                                                                                            <  1:3273608/150‑1 (MQ=255)

                                                      tCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGAt                                                                                            <  2:2148061/150‑1 (MQ=255)

                                                       cGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATc                                                                                           <  1:1166395/150‑1 (MQ=255)

                                                         gtgtTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCg                                                                                         <  1:3510283/150‑1 (MQ=255)

                                                          tgtTGAAGCGCTAATAGTGGTCACATAATAAGTTTCGATGTGGTAAAACACGTCGCGGAAGTAATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGAAGGTTTGCCACTTCCAGGCCTGCTTTCAGATCCgg                                                                                        >  2:1008412/1‑150 (MQ=255)

                                                           gtTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGt                                                                                       >  1:673200/1‑150 (MQ=255)

                                                            ttGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTg                                                                                      <  1:885783/150‑1 (MQ=255)

                                                                aGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAAcc                                                                                  <  2:1181824/150‑1 (MQ=255)

                                                                 gcgcGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAAccc                                                                                 <  1:3111290/150‑1 (MQ=255)

                                                                  cgcgAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCg                                                                                >  1:501983/1‑150 (MQ=255)

                                                                  cgcgAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCg                                                                                >  1:1295449/1‑150 (MQ=255)

                                                                    cgAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGc                                                                              >  2:3863046/1‑150 (MQ=255)

                                                                     gAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCa                                                                             <  1:305293/150‑1 (MQ=255)

                                                                      aaTAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAg                                                                            <  1:2707531/150‑1 (MQ=255)

                                                                      aaTAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAg                                                                            >  1:73579/1‑150 (MQ=255)

                                                                       aTAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGa                                                                           >  2:2463491/1‑150 (MQ=255)

                                                                        tAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGAc                                                                          >  2:2290582/1‑150 (MQ=255)

                                                                        tAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGAc                                                                          >  2:1583567/1‑150 (MQ=255)

                                                                         aGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGAcc                                                                         >  1:3629033/1‑150 (MQ=255)

                                                                          gTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCt                                                                        <  1:2826465/150‑1 (MQ=255)

                                                                          gTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCt                                                                        <  2:1605769/150‑1 (MQ=255)

                                                                             gTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACa                                                                     <  2:3110794/150‑1 (MQ=255)

                                                                             gTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACa                                                                     <  2:1041176/150‑1 (MQ=255)

                                                                             gTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACa                                                                     <  1:4336591/150‑1 (MQ=255)

                                                                                      tAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAg                                                            <  2:3181536/150‑1 (MQ=255)

                                                                                        aGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAgtg                                                          <  1:2462238/150‑1 (MQ=255)

                                                                                             cGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACt                                                     <  2:4164991/150‑1 (MQ=255)

                                                                                              gATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTg                                                    >  2:1967843/1‑150 (MQ=255)

                                                                                              gATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTg                                                    >  2:2680159/1‑150 (MQ=255)

                                                                                               aTGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCTCTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGc                                                   <  2:4252176/150‑1 (MQ=255)

                                                                                                tgtgGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCg                                                  <  2:1211945/150‑1 (MQ=255)

                                                                                                     tAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGccac                                             >  1:3807840/1‑150 (MQ=255)

                                                                                                       aaTCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGccacca                                           >  1:4122941/1‑150 (MQ=255)

                                                                                                       aaTCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGccacca                                           <  2:3787198/150‑1 (MQ=255)

                                                                                                       aaTCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGccacca                                           <  2:1303975/150‑1 (MQ=255)

                                                                                                         tCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAgg                                         <  1:1095738/150‑1 (MQ=255)

                                                                                                            cGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCtt                                      >  1:165905/1‑150 (MQ=255)

                                                                                                               cgcgGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCag                                   <  1:1533402/150‑1 (MQ=255)

                                                                                                                gcgGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCaga                                  >  2:3052544/1‑150 (MQ=255)

                                                                                                                      ggCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTc                            >  1:4426987/1‑150 (MQ=255)

                                                                                                                      ggCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTc                            >  1:2837647/1‑150 (MQ=255)

                                                                                                                       gCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTcc                           <  1:2746386/150‑1 (MQ=255)

                                                                                                                         aTCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTACCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTg                         >  1:3047986/1‑150 (MQ=255)

                                                                                                                            cAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATc                      >  2:2934599/1‑150 (MQ=255)

                                                                                                                            cAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATc                      >  2:4473221/1‑150 (MQ=255)

                                                                                                                             aGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCt                     <  2:3403536/150‑1 (MQ=255)

                                                                                                                             aGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCt                     <  2:1784280/150‑1 (MQ=255)

                                                                                                                              gACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCtt                    <  2:655895/150‑1 (MQ=255)

                                                                                                                               aCCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTc                   >  1:3715379/1‑150 (MQ=255)

                                                                                                                               aCCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTc                   >  1:3855459/1‑150 (MQ=255)

                                                                                                                                  ttCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCAc                >  2:4023257/1‑150 (MQ=255)

                                                                                                                                  ttCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCATCAGAGCGTTCCTGATCTTCCAc                >  1:1864760/1‑150 (MQ=255)

                                                                                                                                      tGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGa            >  2:4423757/1‑150 (MQ=255)

                                                                                                                                         aCAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACgg         <  2:543269/150‑1 (MQ=255)

                                                                                                                                          cAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACggg        <  1:1464857/150‑1 (MQ=255)

                                                                                                                                               aagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCg   <  1:4363098/150‑1 (MQ=255)

                                                                                                                                                agttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGt  >  2:4501087/1‑150 (MQ=255)

                                                                                                                                                agttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGt  >  1:2224334/1‑150 (MQ=255)

CGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGT  >  CP009273/693592‑693885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`