Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 754,572 G→T 100% E137D (GAG→GAT)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273754,5720GT97.6% 942.1 / 12.2 287E137D (GAG→GAT) sucA2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (1/6);  new base T (135/144);  total (137/150)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 287 total aligned reads displayed.

AGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACA  >  CP009273/754427‑754718
                                                                                                                                                 |                                                                                                                                                  
aGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCt                                                                                                                                                <  2:29368/150‑1 (MQ=255)
  gTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTc                                                                                                                                              <  2:2584531/150‑1 (MQ=255)
     aaaGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAAc                                                                                                                                           <  1:3048922/150‑1 (MQ=255)
        gTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTc                                                                                                                                        <  1:4420593/150‑1 (MQ=255)
         tCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCg                                                                                                                                       >  2:1103170/1‑150 (MQ=255)
         tCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTACCAGGATACCTTCAACGTCg                                                                                                                                       >  1:546471/1‑150 (MQ=255)
           cTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGt                                                                                                                                     <  1:2254340/150‑1 (MQ=255)
            tGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGtt                                                                                                                                    >  1:463075/1‑150 (MQ=255)
            tGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGtt                                                                                                                                    <  2:3506132/150‑1 (MQ=255)
              cAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCa                                                                                                                                  >  2:3755026/1‑150 (MQ=255)
               aGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCAt                                                                                                                                 <  1:1962873/150‑1 (MQ=255)
                gCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCAtt                                                                                                                                <  2:657459/150‑1 (MQ=255)
                    aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGcc                                                                                                                            >  2:2268715/1‑150 (MQ=255)
                      tAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAg                                                                                                                          <  2:2878171/150‑1 (MQ=255)
                      tAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAg                                                                                                                          <  2:3678854/150‑1 (MQ=255)
                          gCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGc                                                                                                                      <  2:3024505/150‑1 (MQ=255)
                          gCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGc                                                                                                                      <  2:2379680/150‑1 (MQ=255)
                           cATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCa                                                                                                                     >  2:4244040/1‑150 (MQ=255)
                             tACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCaaa                                                                                                                   >  2:3387642/1‑150 (MQ=255)
                              aCCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAg                                                                                                                  <  1:140145/150‑1 (MQ=255)
                              aCCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAg                                                                                                                  <  2:2079705/150‑1 (MQ=255)
                                 gCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGaaa                                                                                                               >  1:934338/1‑150 (MQ=255)
                                 gCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGaaa                                                                                                               <  2:3949279/150‑1 (MQ=255)
                                  cTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAc                                                                                                              >  1:3523831/1‑150 (MQ=255)
                                  cTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAc                                                                                                              >  1:2417807/1‑150 (MQ=255)
                                   ttCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAcc                                                                                                             >  1:3766872/1‑150 (MQ=255)
                                    tCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCa                                                                                                            >  1:1239987/1‑150 (MQ=255)
                                       gTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGa                                                                                                         >  1:4381289/1‑150 (MQ=255)
                                       gTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGa                                                                                                         <  1:3951988/150‑1 (MQ=255)
                                        tGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGTCAGCAAGATAAAGTGGCCGATCTGGATCCGTCATTCCACGATCTGACCGAAGCAGACTTCAAGGATAACTTCAAAGTCGGTTCATTTGCAAGCGGCAAAGAAACCATGaa                                                                                                        >  1:2339069/1‑150 (MQ=255)
                                        tGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGaa                                                                                                        <  2:3982173/150‑1 (MQ=255)
                                         ggTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGaaa                                                                                                       <  1:2544921/150‑1 (MQ=255)
                                            cACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTc                                                                                                    >  2:2163564/1‑150 (MQ=255)
                                            cACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTc                                                                                                    <  2:1482187/150‑1 (MQ=255)
                                             aCCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCg                                                                                                   >  2:1972379/1‑150 (MQ=255)
                                                 gCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGa                                                                                               <  1:1553790/150‑1 (MQ=255)
                                                 gCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGa                                                                                               >  2:3263868/1‑150 (MQ=255)
                                                   aTGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgc                                                                                             >  1:3193807/1‑150 (MQ=255)
                                                   aTGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgc                                                                                             >  1:18877/1‑150 (MQ=255)
                                                    tGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgct                                                                                            <  2:1570745/150‑1 (MQ=255)
                                                    tGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCT‑GATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgctg                                                                                           >  2:1901959/1‑150 (MQ=255)
                                                      cGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgctgc                                                                                          >  1:3076875/1‑150 (MQ=255)
                                                       gAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgctgct                                                                                         <  2:4228829/150‑1 (MQ=255)
                                                       gAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgctgct                                                                                         >  2:454757/1‑150 (MQ=255)
                                                         aTCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTgg                                                                                       >  2:2293534/1‑150 (MQ=255)
                                                          tctcGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGa                                                                                      >  2:2745926/1‑150 (MQ=255)
                                                           ctcGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGaa                                                                                     <  1:4118911/150‑1 (MQ=255)
                                                           ctcGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGaa                                                                                     >  2:1042865/1‑150 (MQ=255)
                                                           ctcGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGaa                                                                                     <  1:866501/150‑1 (MQ=255)
                                                              gATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGcc                                                                                  >  1:2394300/1‑150 (MQ=255)
                                                               aTCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGccc                                                                                 <  2:441874/150‑1 (MQ=255)
                                                                tCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCt                                                                                <  2:3064039/150‑1 (MQ=255)
                                                                   gCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCaa                                                                             <  2:1365410/150‑1 (MQ=255)
                                                                        gACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCaaa                                                                        >  2:4123479/1‑150 (MQ=255)
                                                                          cTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAAcc                                                                      >  1:522668/1‑150 (MQ=255)
                                                                           tgtgGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCt                                                                     <  1:2198011/150‑1 (MQ=255)
                                                                           tgtgGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCt                                                                     <  1:4554863/150‑1 (MQ=255)
                                                                            gtgGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTa                                                                    >  1:3989415/1‑150 (MQ=255)
                                                                             tgGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTAc                                                                   >  1:2698976/1‑150 (MQ=255)
                                                                                  gcaAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCgg                                                              >  1:3816989/1‑150 (MQ=255)
                                                                                     aGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGccc                                                           >  2:181021/1‑150 (MQ=255)
                                                                                         aaaGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGAtt                                                       >  2:3391979/1‑150 (MQ=255)
                                                                                          aaGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTg                                                      >  1:1042566/1‑150 (MQ=255)
                                                                                          aaGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTg                                                      <  2:3608615/150‑1 (MQ=255)
                                                                                          aaGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTg                                                      <  2:3878617/150‑1 (MQ=255)
                                                                                             tGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTg                                                   <  2:481756/150‑1 (MQ=255)
                                                                                                 cGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGa                                               >  2:2706764/1‑150 (MQ=255)
                                                                                                  gATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAg                                              <  2:3731898/150‑1 (MQ=255)
                                                                                                  gATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAg                                              <  2:2199057/150‑1 (MQ=255)
                                                                                                         aTCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAACATGAAACTCGGCGAGCTGCTGGAATCCCTCAAGCAAACCTACTGCGGACCGATTGGTGCCGAGTATATGc                                       >  1:3382724/1‑150 (MQ=255)
                                                                                                           ccGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGcac                                     <  2:2471456/150‑1 (MQ=255)
                                                                                                           ccGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGcac                                     <  2:312672/150‑1 (MQ=255)
                                                                                                            cGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGcaca                                    <  1:1816615/150‑1 (MQ=255)
                                                                                                             gTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACAt                                   <  2:3404058/150‑1 (MQ=255)
                                                                                                                  tCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCa                              <  2:1285186/150‑1 (MQ=255)
                                                                                                                       gATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCAcc                         >  1:3244017/1‑150 (MQ=255)
                                                                                                                        aTCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCg                        <  2:30879/150‑1 (MQ=255)
                                                                                                                         tCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCga                       <  2:2356426/150‑1 (MQ=255)
                                                                                                                         tCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCga                       <  1:2028864/150‑1 (MQ=255)
                                                                                                                          cTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaa                      >  2:4135152/1‑150 (MQ=255)
                                                                                                                           tGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaag                     <  2:4622744/150‑1 (MQ=255)
                                                                                                                               cGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaa                 >  1:2617419/1‑150 (MQ=255)
                                                                                                                                gAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                >  2:1703038/1‑150 (MQ=255)
                                                                                                                                gAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                >  1:1678276/1‑150 (MQ=255)
                                                                                                                                  aGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACg              <  1:3787584/150‑1 (MQ=255)
                                                                                                                                    cAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCt            <  2:3837096/150‑1 (MQ=255)
                                                                                                                                     ataCTTCCACGAAACCTTCAACGTCCGTTCAATTGCCACCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGCCCCGATTGGTGCCGAGAATATGAACATTACCAGCACCGAATAAAAACGCTg           >  2:2758907/3‑150 (MQ=255)
                                                                                                                                     aGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTg           >  1:3697638/1‑150 (MQ=255)
                                                                                                                                      gACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTgg          <  1:2508880/150‑1 (MQ=255)
                                                                                                                                      gACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTgg          <  2:3994011/150‑1 (MQ=255)
                                                                                                                                      gACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTgg          <  1:2896301/150‑1 (MQ=255)
                                                                                                                                      gACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTgg          <  2:354490/150‑1 (MQ=255)
                                                                                                                                      gACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTgg          >  1:606130/1‑150 (MQ=255)
                                                                                                                                        cTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGAt        >  2:728503/1‑150 (MQ=255)
                                                                                                                                         ttCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATc       >  2:2126934/1‑150 (MQ=255)
                                                                                                                                         ttCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATc       >  1:419390/1‑150 (MQ=255)
                                                                                                                                         ttCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACAGTACCAGCACCGAAGAAAAACGCTGGATc       >  1:419474/1‑150 (MQ=255)
                                                                                                                                          tCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATcc      <  1:1142586/150‑1 (MQ=255)
                                                                                                                                           ccAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCa     <  1:755384/150‑1 (MQ=255)
                                                                                                                                             aGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAAc   >  2:2977324/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACA  >  CP009273/754427‑754718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑