Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 772,664 A→C 9.6% K289N (AAA→AAC)  tolA → membrane anchored protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273772,6640AC 9.6% 564.8 / 15.9 241K289N (AAA→AAC) tolAmembrane anchored protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base A (142/71);  new base C (3/25);  total (145/96)
Fisher's exact test for biased strand distribution p-value = 1.24e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAA  >  CP009273/772515‑772813
                                                                                                                                                     |                                                                                                                                                     
gcagAGAAAGCTGAAGCCTAAAAAAAAGACCAATAAAACGCGGCTGATGAAAAGGAAGCCGCTTATAAGAACGCAGCGGAATAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCaaaa                                                                                                                                                       <  1:1988200/150‑1 (MQ=255)
  agacAACGCTGCTGCCTACAAAAACGCCGCACAAACCGCGGGTGCTTAAAAGCCAGCAGCTTATAAGAACGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGc                                                                                                                                                     <  1:884785/150‑1 (MQ=255)
   gagaACGCTGCAGCCGACAAAAAAGCAGCATAAAAAGCGGCTGCTGAAAAGGCAGCAGCTTATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCg                                                                                                                                                    <  2:871787/150‑1 (MQ=255)
    agaAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCgg                                                                                                                                                   >  1:1890172/1‑150 (MQ=255)
      aaaGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGcc                                                                                                                                                 >  2:2311589/1‑150 (MQ=255)
       aaGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCACAAGCGGCCg                                                                                                                                                >  1:292606/1‑150 (MQ=255)
        aGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCCAAAGCGGCCGc                                                                                                                                               >  2:1224097/1‑150 (MQ=255)
          cTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCag                                                                                                                                             >  1:4617224/1‑150 (MQ=255)
                cctaCAAAAACCCAGAATAAAATGCGGCTGCTTAAACGGCAGCTGCTGATAAGAACGCTGCGGCATAAAAAGCCGCCGCAGAAAAAAAAGCGGCAGCGGAAAACGCTGCTGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAg                                                                                                                                       <  1:4226107/147‑1 (MQ=255)
                ccGAAAAAAAAGCACCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATCAGAAATCAGCGGCAGAAAAATCCGCCGCAGACCAAAAAGCGGCAGCGGCAAATGCTGCTGCTGAAATAGACGCTGCAGAACTCGCGGCCGAAGTGGCAg                                                                                                                                       >  2:3980308/1‑150 (MQ=25)
                  gACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgat                                                                                                                                     >  1:665084/1‑150 (MQ=255)
                   aaaaaaaaGCAGCATAAAATGCGGCTGCTTAAATGGCTGCTGCTTATAATAAAGCCGGGGATGAAAAAGCCGCCGCAGAAAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgatg                                                                                                                                    <  2:2408927/148‑1 (MQ=255)
                   aCAAAAAATCCGAAGAAAACGCGGCTGCTGAAACGGCTGAAGCTTATAAGAAAGCAGCGGCACAAACAGCCGCCGCAGAAAAAAAAGCGGCAGCGGCAACAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgatg                                                                                                                                    <  2:1706892/150‑1 (MQ=255)
                   aCAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgatg                                                                                                                                    >  2:2486020/1‑150 (MQ=255)
                    cAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGATGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgatga                                                                                                                                   >  2:3113277/1‑150 (MQ=255)
                     aaaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgatgat                                                                                                                                  >  2:3159342/1‑150 (MQ=255)
                     aaaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgatgat                                                                                                                                  >  1:3623244/1‑150 (MQ=255)
                      aaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGata                                                                                                                                 <  2:4604366/150‑1 (MQ=255)
                      aaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGata                                                                                                                                 >  1:1212660/1‑150 (MQ=255)
                      aaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGata                                                                                                                                 >  1:3530748/1‑150 (MQ=255)
                          cccagcagAAAACGCGGCTGCTGAAAAGGCAGCAGCTGATAACAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATAtttt                                                                                                                             <  2:2104957/148‑1 (MQ=255)
                          agcagcAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATAtttt                                                                                                                             >  1:610921/1‑150 (MQ=255)
                           gcagcaGAAAAAGCGGCTGCTTAAAAGGCAGAAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTc                                                                                                                            <  2:3821387/150‑1 (MQ=255)
                            cagcagAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCg                                                                                                                           >  1:970207/1‑150 (MQ=255)
                             agcagAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAATAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCgg                                                                                                                          <  2:2287026/150‑1 (MQ=255)
                               cagAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTg                                                                                                                        >  2:203997/1‑150 (MQ=255)
                                   aaacGCGGCTGCTTAACTGGCTGCTGCTTATACTAACGCCGCGGCAGAAAACGCCGCCGCACACAAAAACGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCt                                                                                                                    <  1:3341191/150‑1 (MQ=255)
                                   aaaaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCt                                                                                                                    >  1:2126366/1‑150 (MQ=255)
                                   aaaaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCt                                                                                                                    >  2:1974201/1‑150 (MQ=255)
                                    aaaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTa                                                                                                                   >  1:2823484/1‑150 (MQ=255)
                                      cgCGGCTGCTTAAAAGGCAGCAGCTGATAATAACGCAGGGGCATAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGAAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAg                                                                                                                 <  2:488921/149‑1 (MQ=255)
                                      aGCGGCTGCTGAAACGGCAGCAGCTGATAAGAACGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAACGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAg                                                                                                                 <  2:1115106/150‑1 (MQ=255)
                                       gCGGCTGCTGAAAAGGCAGCAGCTGATAAGTAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAACGCGGCCGCAGAGGCAGATGATCTTTTCGGTGAGCTAAGc                                                                                                                >  2:4165920/1‑150 (MQ=255)
                                       gCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGc                                                                                                                >  2:2064559/1‑150 (MQ=255)
                                         ggCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGctc                                                                                                              >  2:109202/1‑150 (MQ=255)
                                         ggCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGctc                                                                                                              >  2:3669123/1‑150 (MQ=255)
                                           ctgctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTg                                                                                                            >  1:4098355/1‑150 (MQ=255)
                                               tgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTaa                                                                                                        >  1:2339183/1‑150 (MQ=255)
                                                gAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAg                                                                                                       >  1:3798123/1‑150 (MQ=255)
                                                 aaaaGGCCGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGa                                                                                                      >  2:4565527/1‑150 (MQ=255)
                                                       cagcagCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCACTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCAc                                                                                                >  1:1024827/1‑150 (MQ=255)
                                                           agcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGaa                                                                                            >  1:3742151/1‑150 (MQ=255)
                                                               taTAAGAAAGCACCGGCATAAAACGCCGCCCCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTTAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACg                                                                                        <  1:1079243/149‑1 (MQ=255)
                                                                    gAAAGCAGCGGCAGAAAACGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAACGCTGCAGCTGAAAAACCCGCTGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggggg                                                                                   <  2:613245/150‑1 (MQ=255)
                                                                       agcagcGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAgg                                                                                >  2:3731174/1‑150 (MQ=255)
                                                                             ggCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCACAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGaa                                                                          >  2:3166385/1‑150 (MQ=255)
                                                                             ggCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGaa                                                                          >  2:1822189/1‑150 (MQ=255)
                                                                                 taaaaaGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAACGCCGCTGCAGCAAACGCGGCCGCAGCGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAggg                                                                      <  2:1665777/149‑1 (MQ=255)
                                                                                   aaaaGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGaa                                                                    >  2:55410/1‑150 (MQ=255)
                                                                                   aaaaGCCCCCGCAGACAAAAAAGCGGCAGCGTCAAAAGCTGCAGATTAAAAAGCCGCTTAAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGaa                                                                    <  2:433891/150‑1 (MQ=255)
                                                                                   aaaaCCCGCCGCACACAAAAAAGCGGCAGGGTCAAAAGCTGCAGCTTAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGaa                                                                    <  2:433847/150‑1 (MQ=255)
                                                                                        ccgccgCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATg                                                               >  2:1591044/1‑150 (MQ=255)
                                                                                         cgccgcAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGc                                                              >  2:3341011/1‑150 (MQ=255)
                                                                                          gccgcAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCt                                                             >  1:3097588/1‑150 (MQ=255)
                                                                                           ccgcAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCtt                                                            >  1:3600034/1‑150 (MQ=255)
                                                                                            cgcAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTc                                                           <  2:2619193/150‑1 (MQ=255)
                                                                                            cgaaGAAAAAAAAGGGGCTGCGGAAAACGCTGCAGCTTAAAATGCCGCTGCAGAAAAAGCGGCCGCAGAGGCAGATTATATTTTCGGTGAGCTATGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTc                                                           <  1:661477/147‑1 (MQ=255)
                                                                                              cAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGACCAATGCTTCGc                                                         >  2:1954890/1‑150 (MQ=255)
                                                                                               ataaaaaaaaGCGGCAGCGTCAAAAGCTGCAGCTTAAAAACCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGcc                                                        <  2:1335975/146‑1 (MQ=255)
                                                                                                caaaaaaacGGGGCCGCGGCAAACCGTGCCGCTCAAAACGCCGCTGCAGAAACCGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCt                                                       <  1:3708287/147‑1 (MQ=255)
                                                                                                 aCAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTg                                                      >  1:168769/1‑150 (MQ=255)
                                                                                                  cAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGC‑AAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCTCCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                    <  2:2674976/150‑1 (MQ=255)
                                                                                                   aaaaacGGGGCAGCGCAAAAAGCTCCAGCTTAAAACGCCGCGGCAGAAACCGCGGCCGCAGTGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                    <  1:4385493/150‑1 (MQ=255)
                                                                                                   aaaaaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                    >  1:1309051/1‑150 (MQ=255)
                                                                                                    aaaaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCg                                                   >  1:3455233/1‑150 (MQ=255)
                                                                                                         gcggcagcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCGGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGt                                              <  2:1787296/150‑1 (MQ=255)
                                                                                                         gcgccagccccaAACGCTGCCCTTAAAAACCCCCCTCCACCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAATAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGt                                              <  1:2693765/150‑1 (MQ=255)
                                                                                                          cggcagcggcaAAAGCTGCAGCTTAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTg                                             <  2:1205778/150‑1 (MQ=255)
                                                                                                              agcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTaa                                         >  1:4557/1‑150 (MQ=255)
                                                                                                                 ggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATAc                                      <  2:3465118/150‑1 (MQ=255)
                                                                                                                   caAACGCTGCAGCTGAAAACGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTa                                    <  2:84344/150‑1 (MQ=255)
                                                                                                                    aaaaGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaa                                   >  1:158766/1‑150 (MQ=255)
                                                                                                                       aGCGGCAGCTGAAAACGCCGCTGCAGCAACCGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaaaa                                <  2:3224917/150‑1 (MQ=255)
                                                                                                                         cggCAGCTTAAAACCCCGCTGCCGAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACa                              <  1:1781324/148‑1 (MQ=255)
                                                                                                                          tGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACaa                             <  2:1890172/150‑1 (MQ=255)
                                                                                                                           ccacctcaaaaaGCCGCGGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAAt                            <  1:243206/143‑1 (MQ=255)
                                                                                                                              gCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgc                         <  2:3033543/150‑1 (MQ=255)
                                                                                                                               cTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgcg                        <  2:2306279/150‑1 (MQ=255)
                                                                                                                                 gAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCa                      >  2:2992713/1‑150 (MQ=255)
                                                                                                                                 caaaaaGCCGCTGCAGCAAAAGCGGCAAAAGAGGCAGAGCAACTATAAGTATAGCTAAGCTCTGATAAGAATGCAAATAAAAAGGTGCGAGGGGAGAAAGGGACCAATTAATCGCCTGAAGGGAGTTCTCATAATAGAAAAAATGGCGCa                      >  2:2206553/2‑150 (MQ=255)
                                                                                                                                  aaaaccccGCTCCACAAAAAGCGGCCGCAGAGGCAGATTATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCAt                     <  1:1166624/144‑1 (MQ=255)
                                                                                                                                  aaaacGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCAt                     <  2:2092477/150‑1 (MQ=255)
                                                                                                                                  aaaaaGCCGCTGCCGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTACGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCAt                     <  1:2180493/150‑1 (MQ=255)
                                                                                                                                  aaaaaGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCAt                     <  2:1499854/150‑1 (MQ=255)
                                                                                                                                  aaaaaGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCAt                     >  2:1266137/1‑150 (MQ=255)
                                                                                                                                   aaaaGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATc                    >  2:4478910/1‑150 (MQ=255)
                                                                                                                                    aaaGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCa                   >  2:3387109/1‑150 (MQ=255)
                                                                                                                                     aaGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAg                  >  1:1233017/1‑150 (MQ=255)
                                                                                                                                      aGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAgg                 <  2:1589935/150‑1 (MQ=255)
                                                                                                                                      aGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAgg                 >  2:1280337/1‑150 (MQ=255)
                                                                                                                                      aGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAgg                 >  1:2546820/1‑150 (MQ=255)
                                                                                                                                       gCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCTGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGTGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAggg                >  1:4618048/1‑150 (MQ=255)
                                                                                                                                           cTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCg            <  2:2818772/150‑1 (MQ=255)
                                                                                                                                              cagcaAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGata         >  2:3528226/1‑150 (MQ=255)
                                                                                                                                              cagcaAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGata         >  2:4066687/1‑150 (MQ=255)
                                                                                                                                              cagcaAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGata         >  2:652684/1‑150 (MQ=255)
                                                                                                                                               agcaAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGatat        >  2:924678/1‑150 (MQ=255)
                                                                                                                                                 caAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCa      <  1:1477220/150‑1 (MQ=255)
                                                                                                                                                   aaaGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAAAGCATCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAAt    >  1:2074562/1‑150 (MQ=255)
                                                                                                                                                     aGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAATAATGCACCTAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATaa  <  1:396605/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAA  >  CP009273/772515‑772813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑