Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 776,049 C→A 17.6% noncoding (40/76 nt) lysT → tRNA‑Lys

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273776,0490CA17.6% 609.3 / 80.3 208noncoding (40/76 nt)lysTtRNA‑Lys
Reads supporting (aligned to +/- strand):  ref base C (70/101);  new base A (20/17);  total (90/118)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

AAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGT  >  CP009273/775900‑776195
                                                                                                                                                     |                                                                                                                                                  
aaGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATc                                                                                                                                                    >  2:2014061/1‑150 (MQ=255)
aaGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATc                                                                                                                                                    >  2:3221690/1‑150 (MQ=255)
  gCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCaa                                                                                                                                                  >  1:4597271/1‑150 (MQ=255)
   cAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAt                                                                                                                                                 <  1:2902547/150‑1 (MQ=255)
    aGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAtt                                                                                                                                                <  2:64701/150‑1 (MQ=255)
    aGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAtt                                                                                                                                                <  2:1076746/150‑1 (MQ=255)
     gTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTg                                                                                                                                               <  1:4266694/150‑1 (MQ=255)
      ttGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTgg                                                                                                                                              <  1:2436192/150‑1 (MQ=255)
         aGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCg                                                                                                                                           <  2:3268180/150‑1 (MQ=255)
         aGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCg                                                                                                                                           <  1:1950617/150‑1 (MQ=255)
         aGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCg                                                                                                                                           >  1:1053984/1‑150 (MQ=255)
            gATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAg                                                                                                                                        >  2:1452715/1‑150 (MQ=255)
             aTCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAgg                                                                                                                                       <  2:3681190/150‑1 (MQ=255)
                tACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTc                                                                                                                                    <  1:4207814/150‑1 (MQ=255)
                 aCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCg                                                                                                                                   >  2:169755/1‑150 (MQ=255)
                   aTCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGaa                                                                                                                                 >  1:783545/1‑150 (MQ=255)
                    tCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAAt                                                                                                                                <  1:3662576/150‑1 (MQ=255)
                    tCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAAt                                                                                                                                <  1:3662456/150‑1 (MQ=255)
                    tCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAAt                                                                                                                                <  1:742272/150‑1 (MQ=255)
                        aaTTTTTGTTGCGCTCAAGTCTGATATCAGTAATATATGCCGCCGTTTCCACGGGATATCAAACAAACCGAAAGAAACGATAAAGTGGGTAGTTAGCTCAGTTGGTAGAGCAGTTGAGTTTTAATCAATTGGTCGCTGGTTCGAATCCTg                                                                                                                            <  2:4223198/150‑1 (MQ=37)
                        aaTTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTg                                                                                                                            <  1:3993762/150‑1 (MQ=255)
                         aTTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACG‑AAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTgaa                                                                                                                          >  2:4617383/1‑148 (MQ=255)
                           ttttGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCAc                                                                                                                         >  1:4434811/1‑150 (MQ=255)
                             ttgttgCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCAGCACGa                                                                                                                       >  2:3608791/1‑150 (MQ=255)
                                 tgCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACcca                                                                                                                   >  1:2337040/1‑150 (MQ=255)
                                     cTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACTACCCACCaa                                                                                                               >  1:3634237/1‑150 (MQ=255)
                                      tCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAAt                                                                                                              <  2:2089678/150‑1 (MQ=255)
                                        aaGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCg                                                                                                            >  1:3888920/1‑150 (MQ=255)
                                        aaGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCg                                                                                                            >  1:3952177/1‑150 (MQ=255)
                                           tCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTa                                                                                                         >  2:4456612/1‑150 (MQ=255)
                                             tGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAg                                                                                                       >  2:1723208/1‑150 (MQ=255)
                                              gAAATCAGTAATATATGCCGCCGTTGCCACGTGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCATTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAgg                                                                                                      <  2:3998979/150‑1 (MQ=255)
                                                aaTCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGATGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTg                                                                                                    >  1:247913/1‑150 (MQ=255)
                                                 aTCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTgg                                                                                                   <  1:1197989/150‑1 (MQ=255)
                                                 aTCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTgg                                                                                                   <  1:1197859/150‑1 (MQ=255)
                                                  tCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGa                                                                                                  <  1:298196/150‑1 (MQ=255)
                                                  tCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGa                                                                                                  <  1:3587104/150‑1 (MQ=255)
                                                  tCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGa                                                                                                  <  1:4035826/150‑1 (MQ=255)
                                                   cAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTGTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGaa                                                                                                 >  2:1324372/1‑150 (MQ=255)
                                                     gTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGc                                                                                               <  2:67187/150‑1 (MQ=255)
                                                      tAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCg                                                                                              <  1:10678/150‑1 (MQ=255)
                                                       aaTATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCgg                                                                                             <  2:804082/150‑1 (MQ=255)
                                                           tatGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGgtagt                                                                                         <  2:2647379/150‑1 (MQ=255)
                                                           tatGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAAACCTGCACGACCCACCAATCGCTAAGGTGGAAGCGgtagt                                                                                         >  1:2497275/1‑150 (MQ=255)
                                                             tGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTaa                                                                                       <  2:4625889/150‑1 (MQ=255)
                                                               ccgccgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTaaaa                                                                                     <  1:415693/150‑1 (MQ=255)
                                                                cgccgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAAc                                                                                    >  1:3447397/1‑150 (MQ=255)
                                                                cgccgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAAc                                                                                    <  2:4213030/150‑1 (MQ=255)
                                                                cgccgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAAc                                                                                    >  1:1640417/1‑150 (MQ=255)
                                                                cgccgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAAc                                                                                    <  2:4121839/150‑1 (MQ=255)
                                                                cgccgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAACCAATTGGTCGCAGGTTTGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAAc                                                                                    <  2:11924/150‑1 (MQ=255)
                                                                 gccgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACg                                                                                   >  2:617254/1‑150 (MQ=255)
                                                                 gccgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACg                                                                                   <  2:726285/150‑1 (MQ=255)
                                                                 gccgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACg                                                                                   <  1:1142594/150‑1 (MQ=255)
                                                                    gTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTATGTACGGTGCGAGCGGGAGTaaagagggg                                                                                >  2:1394015/1‑144 (MQ=18)
                                                                     ttGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGaa                                                                               <  1:26391/150‑1 (MQ=255)
                                                                      tGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAg                                                                              <  2:2739919/150‑1 (MQ=255)
                                                                         cACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGAt                                                                           <  2:3732617/150‑1 (MQ=255)
                                                                            gggATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAAc                                                                        <  2:3392501/150‑1 (MQ=255)
                                                                            gggATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAAc                                                                        >  1:4144910/1‑150 (MQ=255)
                                                                                        aaacCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCa                                                            <  1:1356799/150‑1 (MQ=255)
                                                                                         aacCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCTAAAAGTGGTAATCGGTAGTAAATCGTGAAGGATAACGTTGCGTGAGCac                                                           >  2:1731592/1‑149 (MQ=17)
                                                                                          acCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAAc                                                          >  2:3668194/1‑150 (MQ=255)
                                                                                           ccGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACg                                                         <  2:1526791/150‑1 (MQ=255)
                                                                                             gAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCTTGAGCAACGGc                                                       >  1:1460587/1‑150 (MQ=255)
                                                                                               aaGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGccc                                                     >  1:581682/1‑150 (MQ=255)
                                                                                                aGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCg                                                    >  1:128979/1‑150 (MQ=255)
                                                                                                aGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCg                                                    >  2:3539545/1‑150 (MQ=255)
                                                                                                aGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCg                                                    >  2:459278/1‑150 (MQ=255)
                                                                                                aGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGAATGAGCAACGGCCCg                                                    >  1:1764521/1‑150 (MQ=255)
                                                                                                   aaCGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAg                                                 >  1:1717623/1‑150 (MQ=255)
                                                                                                   aaCGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAg                                                 <  2:3846316/150‑1 (MQ=255)
                                                                                                    aCGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAgg                                                <  1:815478/150‑1 (MQ=255)
                                                                                                     cGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAggg                                               <  2:1861602/150‑1 (MQ=255)
                                                                                                      gAAAAAGTGGGTCGTTAGCTCATTTGGTAGAGCAGTTGACTTTTAATCATTTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGTAGAACGTTGCATGAGCAACGGCCCGAAGGGc                                              <  1:4549923/150‑1 (MQ=21)
                                                                                                         aaaGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCgag                                           <  1:2227603/150‑1 (MQ=255)
                                                                                                         aaaGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAAGTGGAAGCGGTAGTAAAACGTGAAGGATAAAGTTGCATGAGCAACGGCCCGAAGGGCgag                                           >  1:2023228/1‑150 (MQ=255)
                                                                                                          aaGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCgaga                                          <  1:1219854/150‑1 (MQ=255)
                                                                                                            gTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGTTGGAAGCGGTAGTAAAACGTGTAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACg                                        >  2:1589622/1‑150 (MQ=255)
                                                                                                              gggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGaa                                      <  1:1881128/150‑1 (MQ=255)
                                                                                                               ggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAGTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGGAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAg                                     >  2:2983868/1‑150 (MQ=255)
                                                                                                                 tCGTTAGCTCAGTTGGTAGAGCATTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTc                                   <  2:248279/150‑1 (MQ=255)
                                                                                                                  cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCg                                  <  2:133805/150‑1 (MQ=255)
                                                                                                                  cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCg                                  <  1:3334621/150‑1 (MQ=255)
                                                                                                                  cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCg                                  <  2:2912635/150‑1 (MQ=255)
                                                                                                                  cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCg                                  <  2:2912502/150‑1 (MQ=255)
                                                                                                                     tAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGt                               >  2:4392902/1‑150 (MQ=255)
                                                                                                                        cTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATAGCTAAGTAGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACTGACCTAAGGGCGAGACGAAGTCGATTCAt                            >  2:870954/1‑150 (MQ=18)
                                                                                                                         tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATc                           >  1:3094209/1‑150 (MQ=255)
                                                                                                                           aGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCt                         <  2:2695611/150‑1 (MQ=255)
                                                                                                                               ggTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCAc                     <  1:877198/150‑1 (MQ=255)
                                                                                                                                gTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACg                    <  2:4374388/150‑1 (MQ=255)
                                                                                                                                gTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACg                    <  2:3235082/150‑1 (MQ=255)
                                                                                                                                      cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACccac              >  1:687429/1‑150 (MQ=255)
                                                                                                                                           gACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATACCGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTa         <  1:1359065/150‑1 (MQ=255)
                                                                                                                                            aCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATAGATAAGGTGGATGCGGTAGTAAAACGTGAAGGATAACGTTGCGTGAGCAACGGCCCGTAGGGCGAGACGAAGTAGTCTCCAACAGCACGACACACCACTaa        >  2:1003073/1‑150 (MQ=17)
                                                                                                                                             cTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAAc       <  2:696803/150‑1 (MQ=255)
                                                                                                                                             cTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAAc       <  2:697371/150‑1 (MQ=255)
                                                                                                                                             cTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAAc       <  1:2820645/150‑1 (MQ=255)
                                                                                                                                               tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACAt     <  1:3221690/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGT  >  CP009273/775900‑776195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑