Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 784,482 G→T 13.0% I318I (ATC→ATA)  galK ← galactokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273784,4820GT13.0% 817.1 / 65.2 238I318I (ATC→ATA) galKgalactokinase
Reads supporting (aligned to +/- strand):  ref base G (111/96);  new base T (12/19);  total (123/115)
Fisher's exact test for biased strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 240 total aligned reads displayed.

TCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAG  >  CP009273/784333‑784631
                                                                                                                                                     |                                                                                                                                                     
 ctTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGa                                                                                                                                                      >  1:1346179/1‑150 (MQ=255)
 ctTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGa                                                                                                                                                      <  2:3697691/150‑1 (MQ=255)
 ctTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGa                                                                                                                                                      >  1:2563587/1‑150 (MQ=255)
 ctTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGa                                                                                                                                                      >  1:2170789/1‑150 (MQ=255)
   ttAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGAtt                                                                                                                                                    >  1:4153654/1‑150 (MQ=255)
     aaTACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTc                                                                                                                                                  >  2:1105816/1‑150 (MQ=255)
      aTACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCt                                                                                                                                                 >  2:4101931/1‑150 (MQ=255)
       tACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTa                                                                                                                                                >  2:1415004/1‑150 (MQ=255)
        aCCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTAc                                                                                                                                               >  1:1170469/1‑150 (MQ=255)
          cTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCa                                                                                                                                             >  2:4012019/1‑150 (MQ=255)
          cTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCa                                                                                                                                             <  1:2373328/150‑1 (MQ=255)
           tGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCag                                                                                                                                            >  2:2076775/1‑150 (MQ=255)
             tttttGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCagag                                                                                                                                          <  1:1412219/150‑1 (MQ=255)
              ttttGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAgt                                                                                                                                         <  1:4523349/150‑1 (MQ=255)
                 tGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTc                                                                                                                                      >  2:1241222/1‑150 (MQ=255)
                  gCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCa                                                                                                                                     >  1:95966/1‑150 (MQ=255)
                   cTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCaa                                                                                                                                    >  1:873311/1‑150 (MQ=255)
                   cTTCATAATGTTCAGCGACAGCTAGCAAAACCGCAGCCACCAGCACTACCGCGAACAGCGCGACGATACACCCGCCAAATACGCCCCCGCACATGCGTACGCCACCTTTGTCGCCAATCACAGCTATGACGAATACAACCAGAGTGACaa                                                                                                                                    >  2:2565070/1‑150 (MQ=255)
                     tCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAAtt                                                                                                                                  <  1:2640648/150‑1 (MQ=255)
                     tCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAAtt                                                                                                                                  >  1:2826351/1‑150 (MQ=255)
                      cATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAAttt                                                                                                                                 <  1:3171906/150‑1 (MQ=255)
                      cATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAAttt                                                                                                                                 >  2:237149/1‑150 (MQ=255)
                      cATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAAttt                                                                                                                                 >  1:312593/1‑150 (MQ=255)
                       atatTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTg                                                                                                                                <  2:4140558/150‑1 (MQ=255)
                         atTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCg                                                                                                                              <  1:350836/150‑1 (MQ=255)
                         atTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCg                                                                                                                              >  2:2610574/1‑150 (MQ=255)
                         atTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCg                                                                                                                              <  2:2831662/150‑1 (MQ=255)
                         atTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCg                                                                                                                              >  1:3387450/1‑150 (MQ=255)
                           tGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGc                                                                                                                            >  1:1508584/1‑150 (MQ=255)
                              tCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACg                                                                                                                         >  1:2589113/1‑150 (MQ=255)
                               cAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAATTTGCGGCACgg                                                                                                                        >  1:1613427/1‑150 (MQ=255)
                                aGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGt                                                                                                                       <  1:1400943/150‑1 (MQ=255)
                                 gCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTg                                                                                                                      <  1:3259521/150‑1 (MQ=255)
                                  cGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGa                                                                                                                     <  1:924339/150‑1 (MQ=255)
                                   gACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAATTTGCGGCACGGTGAt                                                                                                                    <  2:1612981/150‑1 (MQ=255)
                                   gACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGAt                                                                                                                    <  2:3734178/150‑1 (MQ=255)
                                    aCAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGAtt                                                                                                                   <  1:290267/150‑1 (MQ=255)
                                     cAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAATTTGCGGCACGGTGAttt                                                                                                                  <  1:2993502/150‑1 (MQ=255)
                                               tACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCCATTTGCGGCACGGTGATTTCGAAatcatc                                                                                                        >  2:4503596/1‑150 (MQ=255)
                                               tACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAatcatc                                                                                                        <  1:1847335/150‑1 (MQ=255)
                                                aCGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATcg                                                                                                       <  1:356183/150‑1 (MQ=255)
                                                 cGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATcgc                                                                                                      >  1:2921106/1‑150 (MQ=255)
                                                 cGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATcgc                                                                                                      >  1:2277163/1‑150 (MQ=255)
                                                    caggcaCCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCa                                                                                                   <  1:2451452/150‑1 (MQ=255)
                                                     aggcaCCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCAt                                                                                                  <  2:1544672/150‑1 (MQ=255)
                                                         aCCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATagag                                                                                              >  1:2393892/1‑150 (MQ=255)
                                                           cAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGc                                                                                            <  1:4143854/150‑1 (MQ=255)
                                                             gCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCAt                                                                                          <  2:1745600/150‑1 (MQ=255)
                                                             gCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCAt                                                                                          >  1:858488/1‑150 (MQ=255)
                                                               tctTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATga                                                                                        >  1:3947314/1‑150 (MQ=255)
                                                                ctTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTGCCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATgag                                                                                       >  2:967296/1‑150 (MQ=255)
                                                                ctTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATgag                                                                                       <  2:1671898/150‑1 (MQ=255)
                                                                   ccGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACt                                                                                    <  2:3345522/150‑1 (MQ=255)
                                                                     gggATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTcc                                                                                  >  2:4310387/1‑150 (MQ=255)
                                                                     gggATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTcc                                                                                  >  2:4310449/1‑150 (MQ=255)
                                                                     gggATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTcc                                                                                  <  1:154189/150‑1 (MQ=255)
                                                                          cAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCAt                                                                             <  2:2303153/150‑1 (MQ=255)
                                                                            gcgcGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCa                                                                           <  2:1401113/150‑1 (MQ=255)
                                                                               cgacgaTACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACt                                                                        <  1:2854177/150‑1 (MQ=255)
                                                                                 acgaTACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCg                                                                      <  2:2046058/150‑1 (MQ=255)
                                                                                   gaTACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGcc                                                                    <  2:3813870/150‑1 (MQ=255)
                                                                                     tACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCa                                                                  >  2:3877293/1‑150 (MQ=255)
                                                                                       cAGCCGCCNAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATa                                                                >  1:4225667/1‑150 (MQ=255)
                                                                                        aGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATAc                                                               >  2:3843127/1‑150 (MQ=255)
                                                                                        aGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATAc                                                               >  2:1689/1‑150 (MQ=255)
                                                                                          ccgccAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGt                                                             >  1:1917719/1‑150 (MQ=255)
                                                                                             ccAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTc                                                          <  1:666237/150‑1 (MQ=255)
                                                                                             ccAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTc                                                          >  2:2532036/1‑150 (MQ=255)
                                                                                              cAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCa                                                         <  2:1769097/150‑1 (MQ=255)
                                                                                                 aTCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGt                                                      >  1:522785/1‑150 (MQ=255)
                                                                                                 aTCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGt                                                      >  2:3496142/1‑150 (MQ=255)
                                                                                                   ccgccgccgGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCg                                                    <  2:616017/150‑1 (MQ=255)
                                                                                                   ccgccgccgGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCg                                                    <  2:1333239/150‑1 (MQ=255)
                                                                                                   ccgccgccgGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCg                                                    >  2:874258/1‑150 (MQ=255)
                                                                                                       cgccgGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCtt                                                <  1:1119487/150‑1 (MQ=255)
                                                                                                         ccgGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGc                                              <  1:482991/150‑1 (MQ=255)
                                                                                                          cgGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCt                                             >  1:4442155/1‑150 (MQ=255)
                                                                                                          cgGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCt                                             <  2:1066849/150‑1 (MQ=255)
                                                                                                            gTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTcc                                           >  1:3886655/1‑150 (MQ=255)
                                                                                                             tCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCa                                          <  2:4610374/150‑1 (MQ=255)
                                                                                                                    tACGCCACCTTTGTCGCCAATCACAGCTTTGACTATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCt                                   >  1:3211769/1‑150 (MQ=255)
                                                                                                                     aCGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAgcgcgcgg                                  <  2:1930522/150‑3 (MQ=255)
                                                                                                                     aCGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTg                                  <  2:3272050/150‑1 (MQ=255)
                                                                                                                     aCGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTg                                  <  2:2457508/150‑1 (MQ=255)
                                                                                                                      cGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTgg                                 >  1:2150947/1‑150 (MQ=255)
                                                                                                                         cACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAg                              >  1:759035/1‑150 (MQ=255)
                                                                                                                            cTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCtt                           >  2:27852/1‑150 (MQ=255)
                                                                                                                                gTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAAc                       >  1:649060/1‑150 (MQ=255)
                                                                                                                                gTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAAc                       <  2:69015/150‑1 (MQ=255)
                                                                                                                                gTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAAc                       >  1:3452633/1‑150 (MQ=255)
                                                                                                                                      aaTCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGGTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCg                 >  2:772756/1‑150 (MQ=255)
                                                                                                                                          acaGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCg             <  2:2952175/150‑1 (MQ=255)
                                                                                                                                          acaGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCg             >  2:1147675/1‑150 (MQ=255)
                                                                                                                                            aGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGtt           >  1:1751529/1‑150 (MQ=255)
                                                                                                                                             gCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGttt          >  2:3092317/1‑150 (MQ=255)
                                                                                                                                             gCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGttt          <  2:2273228/150‑1 (MQ=255)
                                                                                                                                              cTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGtttt         >  2:3620033/1‑150 (MQ=255)
                                                                                                                                                ttGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTtca       <  1:526265/150‑1 (MQ=255)
                                                                                                                                                  gACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTtcagt     <  2:3337517/150‑1 (MQ=255)
                                                                                                                                                     gATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTtcagtcag  <  2:312593/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAG  >  CP009273/784333‑784631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑