Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 816,292 A→T 5.4% H15L (CAC→CTC)  ybhM → UPF0005 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273816,2920AT 5.4% 875.8 / 29.9 266H15L (CAC→CTC) ybhMUPF0005 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (157/94);  new base T (11/4);  total (168/98)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

AGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTA  >  CP009273/816144‑816440
                                                                                                                                                    |                                                                                                                                                    
aGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCAc                                                                                                                                                     <  1:2213699/150‑1 (MQ=255)
 gCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACg                                                                                                                                                    <  1:1007887/150‑1 (MQ=255)
  cGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGa                                                                                                                                                   >  1:4121055/1‑150 (MQ=255)
  cGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGa                                                                                                                                                   <  2:3188646/150‑1 (MQ=255)
      aGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGcc                                                                                                                                               >  2:501002/1‑150 (MQ=255)
        cGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAg                                                                                                                                             >  1:3283505/1‑150 (MQ=255)
          ttttCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGa                                                                                                                                           >  1:3685964/1‑150 (MQ=255)
           tttCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGat                                                                                                                                          >  2:257109/1‑150 (MQ=255)
               tGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATGAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTa                                                                                                                                      >  1:1689185/1‑150 (MQ=255)
                gTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTaa                                                                                                                                     <  2:1305901/150‑1 (MQ=255)
                  aTCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAAc                                                                                                                                   >  2:4077464/1‑150 (MQ=255)
                    ccATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACgg                                                                                                                                 >  1:516637/1‑150 (MQ=255)
                    ccATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACgg                                                                                                                                 >  2:4473824/1‑150 (MQ=255)
                        aaaTGCAAATGTATTTTGTATGCGTGTTTTTATATTTCTTGTTGGTGAATATTAATATTTTTTCTAAGTATAACTTCAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACTGtata                                                                                                                             <  2:4548707/150‑1 (MQ=255)
                          aTGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCTCGAGGCCAGGATATTAAACGGTATATg                                                                                                                           <  1:1051886/150‑1 (MQ=255)
                          aTGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATg                                                                                                                           <  2:2508557/150‑1 (MQ=255)
                             cAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCt                                                                                                                        >  2:225159/1‑150 (MQ=255)
                              aaaaGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTc                                                                                                                       <  2:2092340/150‑1 (MQ=255)
                                aaGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCAt                                                                                                                     >  1:3191579/1‑150 (MQ=255)
                                  gTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTa                                                                                                                   >  1:690597/1‑150 (MQ=255)
                                  gTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTa                                                                                                                   >  1:2849001/1‑150 (MQ=255)
                                    aTTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTAcc                                                                                                                 >  2:764150/1‑150 (MQ=255)
                                           aTGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAg                                                                                                          >  1:280741/1‑150 (MQ=255)
                                           aTGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAg                                                                                                          <  2:175651/150‑1 (MQ=255)
                                           aTGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAg                                                                                                          <  2:1807508/150‑1 (MQ=255)
                                              cGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGtt                                                                                                       >  2:836773/1‑150 (MQ=255)
                                                tGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTtg                                                                                                     >  1:1829829/1‑150 (MQ=255)
                                                  gTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTtggt                                                                                                   >  2:161778/1‑150 (MQ=255)
                                                  gTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTtggt                                                                                                   >  1:3898097/1‑150 (MQ=255)
                                                   ttttAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTtggtg                                                                                                  >  1:818646/1‑150 (MQ=255)
                                                      tAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCa                                                                                               >  2:1562001/1‑150 (MQ=255)
                                                      tAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCa                                                                                               >  2:1142176/1‑150 (MQ=255)
                                                       aaaTTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCaa                                                                                              >  1:1927810/1‑150 (MQ=255)
                                                        aaTTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAAc                                                                                             >  1:2363963/1‑150 (MQ=255)
                                                        aaTTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAAc                                                                                             >  2:3763187/1‑150 (MQ=255)
                                                          tttATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACTAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCg                                                                                           >  2:444917/1‑150 (MQ=255)
                                                          tttATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCg                                                                                           >  2:3082270/1‑150 (MQ=255)
                                                           ttATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGc                                                                                          >  1:1420441/1‑150 (MQ=255)
                                                             aTTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAg                                                                                        >  1:94636/1‑150 (MQ=255)
                                                             aTTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAg                                                                                        >  2:3583090/1‑150 (MQ=255)
                                                                gATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGAc                                                                                     >  1:4422820/1‑150 (MQ=255)
                                                                 aTGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACt                                                                                    >  2:3268938/1‑150 (MQ=255)
                                                                  tGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTa                                                                                   <  2:3683078/150‑1 (MQ=255)
                                                                  tGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTa                                                                                   <  1:3273215/150‑1 (MQ=255)
                                                                        atattaatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTgg                                                                             <  1:2054490/150‑1 (MQ=255)
                                                                          attaatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTgggg                                                                           >  1:300882/1‑150 (MQ=255)
                                                                           ttaatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGa                                                                          >  1:2297450/1‑150 (MQ=255)
                                                                            taatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAg                                                                         >  1:3639753/1‑150 (MQ=255)
                                                                            taatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATAATAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCATGGGGAg                                                                         >  1:1319533/1‑150 (MQ=255)
                                                                             aatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGc                                                                        >  1:26194/1‑150 (MQ=255)
                                                                                  tttttCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGt                                                                   >  2:2384415/1‑150 (MQ=255)
                                                                                    tttCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGtat                                                                 >  2:3001216/1‑150 (MQ=255)
                                                                                    tttCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGtat                                                                 <  2:923774/150‑1 (MQ=255)
                                                                                      tCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCTCGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTatat                                                               >  2:2729963/1‑150 (MQ=255)
                                                                                      tCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTatat                                                               >  2:3815388/1‑150 (MQ=255)
                                                                                       cTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGGATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATc                                                              >  2:4370352/1‑150 (MQ=255)
                                                                                         aaGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGa                                                            >  2:695082/1‑150 (MQ=255)
                                                                                          aGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGaa                                                           <  1:3586200/150‑1 (MQ=255)
                                                                                             aTAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAAtt                                                        <  2:4250007/150‑1 (MQ=255)
                                                                                               aaCTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTAc                                                      >  2:1236398/1‑150 (MQ=255)
                                                                                               aaCTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTAc                                                      >  1:598047/1‑150 (MQ=255)
                                                                                                   aaaaGAATGGACTCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCa                                                  >  2:2155213/1‑150 (MQ=255)
                                                                                                   aaaaGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCa                                                  >  1:1661297/1‑150 (MQ=255)
                                                                                                       gAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCa                                              >  2:4349251/1‑150 (MQ=255)
                                                                                                        aaTGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCaa                                             >  2:367008/1‑150 (MQ=255)
                                                                                                         aTGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATAATAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGAAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCaaa                                            >  2:4484381/1‑150 (MQ=255)
                                                                                                            gAATCATACTCGCAAAACAGCAATTAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAAACAAatat                                         <  2:412662/150‑1 (MQ=255)
                                                                                                             aaTCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATg                                        <  1:3421796/150‑1 (MQ=255)
                                                                                                                 aTACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTc                                    >  1:3818711/1‑150 (MQ=255)
                                                                                                                 aTACTCGCAAAACAGAAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTc                                    <  1:4146785/150‑1 (MQ=255)
                                                                                                                  tACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTca                                   >  1:3453572/1‑150 (MQ=255)
                                                                                                                  tACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTca                                   >  1:2740844/1‑150 (MQ=255)
                                                                                                                   aCTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcac                                  >  1:3845298/1‑150 (MQ=255)
                                                                                                                    cTCGCAACACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCGAAGTATATCGAAATTACGGCAACCAAATATGATTcacc                                 <  1:3378551/150‑1 (MQ=255)
                                                                                                                      cGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcaccac                               >  2:659510/1‑150 (MQ=255)
                                                                                                                        cAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCAccacca                             >  1:1605583/1‑150 (MQ=255)
                                                                                                                          aaaCAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAat                           <  2:1451289/150‑1 (MQ=255)
                                                                                                                          aaaCAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAat                           >  1:782363/1‑150 (MQ=255)
                                                                                                                           aaCAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatg                          >  1:2211293/1‑150 (MQ=255)
                                                                                                                            aCAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgt                         >  2:1488819/1‑150 (MQ=255)
                                                                                                                              aGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgtat                       <  2:2880830/150‑1 (MQ=255)
                                                                                                                              aGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgtat                       >  2:420229/1‑150 (MQ=255)
                                                                                                                                  aTAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCg                   >  1:507232/1‑150 (MQ=255)
                                                                                                                                  aTAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCg                   >  1:2830681/1‑150 (MQ=255)
                                                                                                                                    aaaTTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGcc                 <  2:3649557/150‑1 (MQ=255)
                                                                                                                                      aTTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCAt               <  2:516865/150‑1 (MQ=255)
                                                                                                                                         aGATTTTCAGCTCGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAgg            >  1:3218839/1‑150 (MQ=255)
                                                                                                                                         aGATTTTCAGCTCGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAgg            >  1:4052558/1‑150 (MQ=255)
                                                                                                                                         aGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAgg            <  2:3549872/150‑1 (MQ=255)
                                                                                                                                          gATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAggg           <  2:335119/150‑1 (MQ=255)
                                                                                                                                           aTTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGt          >  1:4515635/1‑150 (MQ=255)
                                                                                                                                             tttCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCAAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGtta        >  1:660308/1‑150 (MQ=255)
                                                                                                                                              ttCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttat       >  2:3014194/1‑150 (MQ=255)
                                                                                                                                               tCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttatt      >  2:4130945/1‑150 (MQ=255)
                                                                                                                                               tCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttatt      <  1:3814314/150‑1 (MQ=255)
                                                                                                                                                cAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttatta     >  1:1240440/1‑150 (MQ=255)
                                                                                                                                                 aGCACGAGGCCAGGATATTAACCGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttattat    <  2:2316416/150‑1 (MQ=255)
                                                                                                                                                 aGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttattat    <  1:2944890/150‑1 (MQ=255)
                                                                                                                                                 aGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttattat    <  1:3497921/150‑1 (MQ=255)
                                                                                                                                                 aGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttattat    >  1:553439/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTA  >  CP009273/816144‑816440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑