Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 816,302 G→T 8.6% R18S (AGG→AGT)  ybhM → UPF0005 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273816,3020GT 8.6% 993.1 / 42.9 266R18S (AGG→AGT) ybhMUPF0005 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (152/91);  new base T (18/5);  total (170/96)
Fisher's exact test for biased strand distribution p-value = 1.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 272 total aligned reads displayed.

GTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTATGTA  >  CP009273/816153‑816451
                                                                                                                                                     |                                                                                                                                                     
  tttCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGat                                                                                                                                                     <  1:4088918/150‑1 (MQ=255)
  tttCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGat                                                                                                                                                     <  2:1890631/150‑1 (MQ=255)
     cTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCTCGAGGCCAGGATAtt                                                                                                                                                  <  1:2175626/150‑1 (MQ=255)
       gTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTaa                                                                                                                                                <  2:1305901/150‑1 (MQ=255)
         aTCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAAc                                                                                                                                              >  2:4077464/1‑150 (MQ=255)
           ccATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACgg                                                                                                                                            >  1:516653/1‑150 (MQ=255)
           ccATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACgg                                                                                                                                            >  2:4473825/1‑150 (MQ=255)
           ccATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACgg                                                                                                                                            >  2:4473580/1‑150 (MQ=255)
           ccATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACgg                                                                                                                                            >  2:2321494/1‑150 (MQ=255)
             aTAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCTCGAGGCCAGGATATTAAACGGta                                                                                                                                          >  1:1822303/1‑150 (MQ=255)
               aaaTGCAAATGTATTTTGTATGCGTGTTTTTATATTTCTTGTTGGTGAATATTAATATTTTTTCTAAGTATAACTTCAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACTGtata                                                                                                                                        <  2:4548707/150‑1 (MQ=255)
               aaaTGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGtata                                                                                                                                        <  2:2220304/150‑1 (MQ=255)
                aaTGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTatat                                                                                                                                       <  1:2955454/150‑1 (MQ=255)
                aaTGCAAAAGTATTTTGTATGCGTGGATTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTatat                                                                                                                                       <  2:3753084/150‑1 (MQ=255)
                 aTGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCTCGAGGCCAGGATATTAAACGGTATATg                                                                                                                                      <  1:1051885/150‑1 (MQ=255)
                 aTGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATg                                                                                                                                      <  2:247309/150‑1 (MQ=255)
                   gCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGc                                                                                                                                    >  1:3670059/1‑150 (MQ=255)
                         gTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTa                                                                                                                              >  1:690606/1‑150 (MQ=255)
                           aTTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTAcc                                                                                                                            >  2:764129/1‑150 (MQ=255)
                                  aTGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAg                                                                                                                     <  2:175651/150‑1 (MQ=255)
                                  aTGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAg                                                                                                                     <  2:1442371/150‑1 (MQ=255)
                                        ggTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTtgg                                                                                                               >  2:2513459/1‑150 (MQ=255)
                                         gTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGTTATATGGCTCATTACCGCTTTAGGTTtggt                                                                                                              <  1:2377635/150‑1 (MQ=255)
                                         gTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTtggt                                                                                                              >  2:161778/1‑150 (MQ=255)
                                         gTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTtggt                                                                                                              >  1:3898097/1‑150 (MQ=255)
                                             tAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCa                                                                                                          >  2:1562001/1‑150 (MQ=255)
                                              aaaTTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCaa                                                                                                         <  1:1850576/150‑1 (MQ=255)
                                              aaaTTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCaa                                                                                                         >  1:935642/1‑150 (MQ=255)
                                                  ttATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGc                                                                                                     >  2:4444393/1‑150 (MQ=255)
                                                  ttATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGc                                                                                                     >  1:1420441/1‑150 (MQ=255)
                                                    aTTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAg                                                                                                   >  2:67825/1‑150 (MQ=255)
                                                    aTTGATGCTGAATATTAATATTATTTCTAAGGATAACTAAAAGACTGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAg                                                                                                   <  1:2310849/150‑1 (MQ=255)
                                                        aTGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACt                                                                                               <  1:1113225/150‑1 (MQ=255)
                                                        aTGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACt                                                                                               >  2:3268938/1‑150 (MQ=255)
                                                         tGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTa                                                                                              >  2:2129671/1‑150 (MQ=255)
                                                               atattaatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTgg                                                                                        <  1:2054490/150‑1 (MQ=255)
                                                                 attaatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTgggg                                                                                      >  1:300882/1‑150 (MQ=255)
                                                                   taatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAg                                                                                    >  1:3639753/1‑150 (MQ=255)
                                                                   taatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATAATAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCATGGGGAg                                                                                    >  1:1319533/1‑150 (MQ=255)
                                                                    aatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGc                                                                                   >  1:26194/1‑150 (MQ=255)
                                                                    aatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGc                                                                                   >  1:4144296/1‑150 (MQ=255)
                                                                     atattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCt                                                                                  <  2:3149243/150‑1 (MQ=255)
                                                                      tattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTa                                                                                 >  1:574093/1‑150 (MQ=255)
                                                                           tttCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGtat                                                                            <  2:923825/150‑1 (MQ=255)
                                                                           tttCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGtat                                                                            >  2:3001216/1‑150 (MQ=255)
                                                                             tCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCTCGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTatat                                                                          >  2:2728813/1‑150 (MQ=255)
                                                                              cTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGGATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATc                                                                         >  2:4370352/1‑150 (MQ=255)
                                                                                    aTAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAAtt                                                                   >  2:798186/1‑150 (MQ=255)
                                                                                      aaCTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTAc                                                                 >  2:516350/1‑150 (MQ=255)
                                                                                       aCTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACg                                                                <  2:3777865/150‑1 (MQ=255)
                                                                                         tAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGc                                                              >  1:3571233/1‑150 (MQ=255)
                                                                                          aaaaGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCa                                                             >  1:1661297/1‑150 (MQ=255)
                                                                                          aaaaGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCa                                                             >  1:61553/1‑150 (MQ=255)
                                                                                           aaaGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCaa                                                            >  1:3720736/1‑150 (MQ=255)
                                                                                           aaaGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCaa                                                            <  2:3666865/150‑1 (MQ=255)
                                                                                           aaaGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCaa                                                            >  1:4544054/1‑150 (MQ=255)
                                                                                            aaGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAAc                                                           <  2:4242875/150‑1 (MQ=255)
                                                                                               aaTGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCACCCaa                                                        >  2:4330940/1‑150 (MQ=255)
                                                                                               aaTGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCaa                                                        >  2:367302/1‑150 (MQ=255)
                                                                                                aTGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCaaa                                                       >  2:3554119/1‑150 (MQ=255)
                                                                                                   gAATCATACTCTCAAAACAGCTATAAATTAGATTTTCAGCACGAGGCCACGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGATAAGTATATCGAAATTACGGCATCCAAttat                                                    <  2:412992/150‑1 (MQ=255)
                                                                                                   gAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAatat                                                    >  2:225937/1‑150 (MQ=255)
                                                                                                    aaTCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATg                                                   <  1:3421796/150‑1 (MQ=255)
                                                                                                      tCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGAt                                                 >  2:1204680/1‑150 (MQ=255)
                                                                                                       cATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGAtt                                                >  1:4157725/1‑150 (MQ=255)
                                                                                                        aTACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTc                                               >  1:3818711/1‑150 (MQ=255)
                                                                                                         tACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTca                                              >  1:3453572/1‑150 (MQ=255)
                                                                                                          aCTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcac                                             >  1:3845298/1‑150 (MQ=255)
                                                                                                           cTCGCAACACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCGAAGTATATCGAAATTACGGCAACCAAATATGATTcacc                                            <  1:3378551/150‑1 (MQ=255)
                                                                                                             cGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcaccac                                          >  1:4460025/1‑150 (MQ=255)
                                                                                                             cGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcaccac                                          >  2:659489/1‑150 (MQ=255)
                                                                                                             cGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcaccac                                          >  1:289717/1‑150 (MQ=255)
                                                                                                             cGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcaccac                                          <  1:3274125/150‑1 (MQ=255)
                                                                                                               cAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCAccacca                                        >  1:1605583/1‑150 (MQ=255)
                                                                                                                 aaaCAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAat                                      >  1:1753146/1‑150 (MQ=255)
                                                                                                                 aaaCAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAat                                      >  1:782363/1‑150 (MQ=255)
                                                                                                                  aaCAGCAATAAATTAGATTTTCAGCTCGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatg                                     >  2:3076136/1‑150 (MQ=255)
                                                                                                                   aCAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgt                                    >  2:1488819/1‑150 (MQ=255)
                                                                                                                     aGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgtat                                  <  2:2880830/150‑1 (MQ=255)
                                                                                                                     aGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgtat                                  >  2:4116507/1‑150 (MQ=255)
                                                                                                                     aGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgtat                                  <  2:886539/150‑1 (MQ=255)
                                                                                                                     aGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgtat                                  >  2:420229/1‑150 (MQ=255)
                                                                                                                     aGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgtat                                  >  2:3649337/1‑150 (MQ=255)
                                                                                                                       cAATAAATTAGATTTTCAGCTCGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgtatgt                                >  1:2827241/1‑150 (MQ=255)
                                                                                                                        aaTAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTc                               >  1:323603/1‑150 (MQ=255)
                                                                                                                         aTAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCg                              >  1:2830681/1‑150 (MQ=255)
                                                                                                                         aTAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCg                              >  1:507232/1‑150 (MQ=255)
                                                                                                                                aGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAgg                       <  1:2763017/150‑1 (MQ=255)
                                                                                                                                 gATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAggg                      <  2:335119/150‑1 (MQ=255)
                                                                                                                                    tttCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGtta                   >  1:660341/1‑150 (MQ=255)
                                                                                                                                      tCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttatt                 >  2:2865335/1‑150 (MQ=255)
                                                                                                                                        aGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGTCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttattat               >  1:2211929/1‑150 (MQ=255)
                                                                                                                                        aGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttattat               <  1:3497919/150‑1 (MQ=255)
                                                                                                                                         gCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttattatt              <  1:3202044/150‑1 (MQ=255)
                                                                                                                                              aGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACttt         >  1:999187/1‑150 (MQ=255)
                                                                                                                                               ggCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTtg        <  2:3955153/150‑1 (MQ=255)
                                                                                                                                                gCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTtgt       >  2:3979191/1‑150 (MQ=255)
                                                                                                                                                   aGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTtgtatg    >  1:1267619/1‑150 (MQ=255)
                                                                                                                                                   aGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTtgtatg    <  1:286843/150‑1 (MQ=255)
                                                                                                                                                   aGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTtgtatg    >  1:3345399/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTATGTA  >  CP009273/816153‑816451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑