Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 825,641 G→T 37.4% A153A (GCC→GCA)  ybiH ← DUF1956 domain‑containing tetR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273825,6410GT37.4% 345.3 / 238.8 232A153A (GCC→GCA) ybiHDUF1956 domain‑containing tetR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (61/84);  new base T (42/44);  total (104/128)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

ATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTC  >  CP009273/825492‑825788
                                                                                                                                                     |                                                                                                                                                   
aTCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAggcg                                                                                                                                                     >  2:1344875/1‑150 (MQ=255)
aTCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAggcg                                                                                                                                                     >  2:3664917/1‑150 (MQ=255)
  cAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAggcggc                                                                                                                                                   >  2:603356/1‑150 (MQ=255)
   aGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCa                                                                                                                                                  >  1:2632613/1‑150 (MQ=255)
     ttCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCCCCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAAt                                                                                                                                                <  1:4513681/150‑1 (MQ=255)
      tCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATc                                                                                                                                               <  1:431663/150‑1 (MQ=255)
      tCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATc                                                                                                                                               <  2:3644557/150‑1 (MQ=255)
       cGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCa                                                                                                                                              <  1:1280550/150‑1 (MQ=255)
        ggTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGAGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCACTCAg                                                                                                                                             >  1:2387/1‑150 (MQ=255)
               tcttcATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCCCCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTc                                                                                                                                      <  1:4098549/150‑1 (MQ=255)
               tcttcATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTc                                                                                                                                      <  2:4522908/150‑1 (MQ=255)
                  tcATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAgg                                                                                                                                   >  1:1561539/1‑150 (MQ=255)
                   cATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGt                                                                                                                                  <  1:248014/150‑1 (MQ=255)
                    aTCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTg                                                                                                                                 <  2:3873202/150‑1 (MQ=255)
                     tCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTgg                                                                                                                                >  2:4101682/1‑150 (MQ=255)
                        aaCGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCtg                                                                                                                             >  1:1945046/1‑150 (MQ=255)
                         aCGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCtgt                                                                                                                            >  1:2107273/1‑150 (MQ=255)
                                 cAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGa                                                                                                                    >  2:1102650/1‑150 (MQ=255)
                                  aGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGAc                                                                                                                   <  1:839148/150‑1 (MQ=255)
                                   gCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACt                                                                                                                  <  2:1468777/150‑1 (MQ=255)
                                     cGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTaa                                                                                                                >  1:3143939/1‑150 (MQ=255)
                                     cGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTaa                                                                                                                <  1:3114030/150‑1 (MQ=255)
                                      ggTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAAt                                                                                                               >  2:3286751/1‑150 (MQ=255)
                                      ggTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAAt                                                                                                               <  2:3792909/150‑1 (MQ=255)
                                        tACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCa                                                                                                             >  1:2123785/1‑150 (MQ=255)
                                        tACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCa                                                                                                             >  1:3192248/1‑150 (MQ=255)
                                          cGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCCCCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCAGTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCAcc                                                                                                           <  1:469788/150‑1 (MQ=255)
                                          cGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGAACATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCAcc                                                                                                           <  2:692302/150‑1 (MQ=255)
                                            tAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTg                                                                                                         >  1:886443/1‑150 (MQ=255)
                                               cAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTc                                                                                                      <  2:3166082/150‑1 (MQ=255)
                                               cAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTc                                                                                                      >  1:689583/1‑150 (MQ=255)
                                                 gAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGt                                                                                                    >  1:3655441/1‑150 (MQ=255)
                                                   nttGTTTCNTTGCCAANACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGNGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGc                                                                                                  >  2:330444/2‑150 (MQ=255)
                                                        ttCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAg                                                                                             <  1:3467189/150‑1 (MQ=255)
                                                        ttCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAg                                                                                             <  1:1566283/150‑1 (MQ=255)
                                                                cAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAgg                                                                                     <  2:2034928/150‑1 (MQ=255)
                                                                cAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAgg                                                                                     <  2:2461966/150‑1 (MQ=255)
                                                                cAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAgg                                                                                     >  2:4502031/1‑150 (MQ=255)
                                                                 aaGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGc                                                                                    >  2:97491/1‑150 (MQ=255)
                                                                  aGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCt                                                                                   <  1:146441/150‑1 (MQ=255)
                                                                  aGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCt                                                                                   >  2:2785273/1‑150 (MQ=255)
                                                                     cGGAACGCCAGAATCTCCCCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGcc                                                                                <  1:4172459/150‑1 (MQ=255)
                                                                     cGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGcc                                                                                <  2:1546429/150‑1 (MQ=255)
                                                                      ggAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCg                                                                               >  1:1119502/1‑150 (MQ=255)
                                                                      ggAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCg                                                                               >  1:2556007/1‑150 (MQ=255)
                                                                       gAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGt                                                                              >  2:3527461/1‑150 (MQ=255)
                                                                         aCGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTgg                                                                            <  1:1106503/150‑1 (MQ=255)
                                                                           gCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGga                                                                          <  2:2053852/150‑1 (MQ=255)
                                                                             cAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGgaga                                                                        <  1:2300270/150‑1 (MQ=255)
                                                                               gAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGgagaga                                                                      >  1:3819900/1‑150 (MQ=255)
                                                                                 aTCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAgc                                                                    >  1:1073718/1‑150 (MQ=255)
                                                                                  tctcACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAgct                                                                   >  1:2077862/1‑150 (MQ=255)
                                                                                  tctcACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAgct                                                                   <  2:1808070/150‑1 (MQ=255)
                                                                                    tcACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAgctgc                                                                 >  1:3414539/1‑150 (MQ=255)
                                                                                       ccAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCa                                                              >  1:244492/1‑150 (MQ=255)
                                                                                       ccAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCa                                                              <  1:4600255/150‑1 (MQ=255)
                                                                                           tCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACggg                                                          <  1:3368121/150‑1 (MQ=255)
                                                                                            cAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGga                                                         <  2:4306880/150‑1 (MQ=255)
                                                                                              aCGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGgaga                                                       <  2:3751657/150‑1 (MQ=255)
                                                                                                   tGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAAc                                                  <  1:3930715/150‑1 (MQ=255)
                                                                                                    gggTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACt                                                 <  2:728792/150‑1 (MQ=255)
                                                                                                       tATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGc                                              >  2:1374325/1‑150 (MQ=255)
                                                                                                          gAAGGATCCTGCGGGTGTCATAGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTACGTGCACCAGGGGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGCAGATAAACTTGCTGa                                           >  2:12400/1‑150 (MQ=255)
                                                                                                          gAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGa                                           >  1:1843504/1‑150 (MQ=255)
                                                                                                          gAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCAGCAATCAGGCGTGTCAGGTGGCTGTGTATCGGACTAATAACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGa                                           >  1:2070569/1‑150 (MQ=255)
                                                                                                           aaGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAg                                          >  1:985087/1‑150 (MQ=255)
                                                                                                           aaGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAg                                          <  1:2909885/150‑1 (MQ=255)
                                                                                                            aGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAgg                                         <  2:2576298/150‑1 (MQ=255)
                                                                                                            aGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAgg                                         <  2:720170/150‑1 (MQ=255)
                                                                                                            aGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAgg                                         >  1:1420619/1‑150 (MQ=255)
                                                                                                            aGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAgg                                         <  1:1354213/150‑1 (MQ=255)
                                                                                                              gATCCTGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGtt                                       <  2:632093/150‑1 (MQ=255)
                                                                                                              gATCATGCGGGTGTCAGTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGtt                                       >  2:3427690/1‑150 (MQ=255)
                                                                                                                tCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGa                                     >  1:4254012/1‑150 (MQ=255)
                                                                                                                 cATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGAc                                    <  1:4124188/150‑1 (MQ=255)
                                                                                                                 cATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGAc                                    <  1:4410218/150‑1 (MQ=255)
                                                                                                                 cATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGAc                                    >  1:4009894/1‑150 (MQ=255)
                                                                                                                    gCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGgt                                 <  2:2567621/150‑1 (MQ=255)
                                                                                                                         tgtCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCAt                            <  1:2428216/150‑1 (MQ=255)
                                                                                                                             aTTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTg                        >  1:2805568/1‑150 (MQ=255)
                                                                                                                                  cGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGa                   >  2:3014354/1‑150 (MQ=255)
                                                                                                                                  cGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGa                   >  2:3452880/1‑150 (MQ=255)
                                                                                                                                  cGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGa                   >  2:2366312/1‑150 (MQ=255)
                                                                                                                                   gTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGag                  >  2:1805961/1‑150 (MQ=255)
                                                                                                                                   gTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGag                  <  2:415861/150‑1 (MQ=255)
                                                                                                                                   gTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGag                  >  2:44719/1‑150 (MQ=255)
                                                                                                                                   gTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGag                  <  2:61413/150‑1 (MQ=255)
                                                                                                                                      gCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGagcag               >  1:897372/1‑150 (MQ=255)
                                                                                                                                       cAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGagcagc              >  2:1142322/1‑150 (MQ=255)
                                                                                                                                        aGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCt             <  1:1877083/150‑1 (MQ=255)
                                                                                                                                         gCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCtt            <  1:517402/150‑1 (MQ=255)
                                                                                                                                          ccGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTa           <  2:2200516/150‑1 (MQ=255)
                                                                                                                                            ggTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAAt         <  2:2310543/150‑1 (MQ=255)
                                                                                                                                             gTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATc        >  2:3727896/1‑150 (MQ=255)
                                                                                                                                               ccAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCAt      <  1:1737332/150‑1 (MQ=255)
                                                                                                                                                cAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATg     >  1:1226444/1‑150 (MQ=255)
                                                                                                                                                 aGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGt    >  2:93818/1‑150 (MQ=255)
                                                                                                                                                  ggctgcAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGtt   <  1:836703/150‑1 (MQ=255)
                                                                                                                                                  ggcggcAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGtt   <  1:2226568/150‑1 (MQ=255)
                                                                                                                                                   gcggcAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTc  <  1:1933761/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTC  >  CP009273/825492‑825788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑