| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 97,521 | C→A | 100% | A90A (GCC→GCA) | murC → | UDP‑N‑acetylmuramate:L‑alanine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 97,521 | 0 | C | A | 96.9% | 876.0 / 15.9 | 261 | A90A (GCC→GCA) | murC | UDP‑N‑acetylmuramate:L‑alanine ligase |
| Reads supporting (aligned to +/- strand): ref base C (5/3); new base A (121/132); total (126/135) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 261 total aligned reads displayed.
TGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCA > CP009273/97374‑97666 | tGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGa > 1:688918/1‑150 (MQ=255) gTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAAcc > 1:4170038/1‑150 (MQ=255) ttATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAAccc < 2:4229998/150‑1 (MQ=255) ttATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAAccc < 2:2660626/150‑1 (MQ=255) atcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAAtt < 1:4068425/150‑1 (MQ=255) atcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAAtt < 2:3703155/150‑1 (MQ=255) atcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAAtt < 2:262603/150‑1 (MQ=255) tcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTg < 1:3746259/150‑1 (MQ=255) gTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTcgccg < 1:1307011/150‑1 (MQ=255) gTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTcgccg > 1:3223380/1‑150 (MQ=255) gATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCAt > 1:269577/1‑150 (MQ=255) gATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCAt > 1:3052282/1‑150 (MQ=255) aGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAgc > 2:1202591/1‑150 (MQ=255) aGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAgc < 2:3127914/150‑1 (MQ=255) gcgcCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAgcg < 1:2505274/150‑1 (MQ=255) cAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTa > 1:3480537/1‑150 (MQ=255) aaaTCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTAt > 1:469962/1‑150 (MQ=255) tCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTcc < 2:1908840/150‑1 (MQ=255) tCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTcc < 2:3976278/150‑1 (MQ=255) tCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTcc > 1:1485663/1‑150 (MQ=255) ccGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCg < 1:2886780/150‑1 (MQ=255) ccGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCg < 1:3814756/150‑1 (MQ=255) ccGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCg < 2:4163881/150‑1 (MQ=255) ggTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGt > 2:4283943/1‑150 (MQ=255) gTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTg > 2:4355836/1‑150 (MQ=255) tCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGa < 2:2090513/150‑1 (MQ=255) cACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGAt < 1:721207/150‑1 (MQ=255) cGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATcc < 2:138675/150‑1 (MQ=255) gcagcaGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCcg < 1:3967568/150‑1 (MQ=255) agcagTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCcgtc > 1:17383/1‑150 (MQ=255) cagTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCcgtcgt > 2:1780634/1‑150 (MQ=255) agTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTg > 2:2054254/1‑150 (MQ=255) ttAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTgtc > 2:3362107/1‑148 (MQ=255) ttAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGcc > 2:3742251/1‑150 (MQ=255) aaTGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGa < 1:2966491/150‑1 (MQ=255) aTGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGaa > 1:984038/1‑150 (MQ=255) tGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGaaa > 1:4531335/1‑150 (MQ=255) gAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAAt > 2:3324126/1‑150 (MQ=255) aaTCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATg > 1:50319/1‑150 (MQ=255) aaTCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATg < 2:3376142/150‑1 (MQ=255) tCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgct < 1:623158/150‑1 (MQ=255) cTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctg < 2:560576/150‑1 (MQ=255) tGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctgg < 2:3947336/150‑1 (MQ=255) tGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctgg > 1:1864870/1‑150 (MQ=255) gggTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctggc > 2:43779/1‑150 (MQ=255) gTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctggctg < 2:3842830/150‑1 (MQ=255) gTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctggctg < 2:1366441/150‑1 (MQ=255) gCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGGGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAg < 1:3560611/150‑1 (MQ=255) gacgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGtt < 1:3263066/150‑1 (MQ=255) gacgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGtt < 2:3863991/150‑1 (MQ=255) acgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTa < 2:3784684/150‑1 (MQ=255) cgaTTTATTTCAACCCTCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTaa < 1:1560693/150‑1 (MQ=255) cgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACACGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTaa < 1:1253535/150‑1 (MQ=255) tttatttCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGc > 1:283840/1‑150 (MQ=255) ttatttCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCg < 1:3303503/150‑1 (MQ=255) atttCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGtt > 2:149302/1‑150 (MQ=255) tttCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGttt > 2:1956078/1‑150 (MQ=255) ttCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGtttt < 2:2504703/150‑1 (MQ=255) cAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCg < 1:1336947/150‑1 (MQ=255) cATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCAt > 1:817923/1‑150 (MQ=255) aTCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATg > 1:1505688/1‑150 (MQ=255) aTCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATg < 2:2728556/150‑1 (MQ=255) aTCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATg < 1:2247993/150‑1 (MQ=255) cGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGc < 1:3201600/150‑1 (MQ=255) gCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCa < 2:2777793/150‑1 (MQ=255) cGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCg < 1:4223910/150‑1 (MQ=255) aaaaCGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCa > 2:2360278/1‑150 (MQ=255) aaaaCGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCa > 2:4439684/1‑150 (MQ=255) aaaaCGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCa > 2:4198269/1‑150 (MQ=255) accgtacgtGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCAtt > 1:2278893/3‑150 (MQ=255) aaCGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCAtt < 2:582957/150‑1 (MQ=255) acgtacgtGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTg > 1:490030/1‑150 (MQ=255) cgtacgtGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGc < 1:2053643/150‑1 (MQ=255) gtacgtGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGAGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGcc > 2:701593/1‑150 (MQ=255) tGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAAc < 1:2533114/150‑1 (MQ=255) gATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACg > 1:3272412/1‑150 (MQ=255) aTGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGc > 1:889697/1‑150 (MQ=255) gCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCAc > 1:2718090/1‑150 (MQ=255) gCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCAc > 2:1315014/1‑150 (MQ=255) gCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTAATCCGGTGATCCGTCGTGCCCAAAAGCTGGCAGAGTTAATGCGTTTTCGTCATGGCATCGCCATAGCCGGAACGCAc > 2:4255625/1‑150 (MQ=255) gCCAGCGTGGTCGTTGTTTCCAGCGCGAGTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCAc < 2:1636221/150‑1 (MQ=255) cAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACgg < 2:4502866/150‑1 (MQ=255) cAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACgg > 2:3267720/1‑150 (MQ=255) aGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGc < 2:1720477/150‑1 (MQ=255) tGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCaaaa > 2:3484332/1‑150 (MQ=255) cGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGAc < 1:1082650/150‑1 (MQ=255) gttgttTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACa > 1:1729310/1‑150 (MQ=255) ttgttTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACaa > 2:4255268/1‑150 (MQ=255) tgttTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAac > 1:4363640/1‑150 (MQ=255) gttTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAacc < 1:2326156/150‑1 (MQ=255) gttTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAacc > 2:3020215/1‑150 (MQ=255) tttCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAacca < 1:4254445/150‑1 (MQ=255) tttCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAacca > 2:4439502/1‑150 (MQ=255) tCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAaccacc > 2:2024387/1‑150 (MQ=255) tCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAaccacc > 1:1986387/1‑150 (MQ=255) tCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTAATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAaccacc < 2:1588080/150‑1 (MQ=255) aGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACcgcg > 1:514255/1‑150 (MQ=255) aGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACcgcg > 2:2485110/1‑150 (MQ=255) cgcgATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGAt < 2:2870547/150‑1 (MQ=255) cgATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATgg > 2:4072559/1‑150 (MQ=255) | TGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCA > CP009273/97374‑97666 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |