Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,240,865 A→T 14.0% K84* (AAA→TAA)  ycgY → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,240,8650AT14.0% 668.7 / 87.0 228K84* (AAA→TAA) ycgYuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (102/94);  new base T (22/10);  total (124/104)
Fisher's exact test for biased strand distribution p-value = 8.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 248 total aligned reads displayed.

GATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACAT  >  CP009273/1240717‑1241012
                                                                                                                                                    |                                                                                                                                                   
gATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCaaaaa                                                                                                                                                    >  2:1812325/1‑150 (MQ=255)
gATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCaaaaa                                                                                                                                                    >  2:1242563/1‑150 (MQ=255)
  tCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCaaaaaaa                                                                                                                                                  >  1:4369718/1‑150 (MQ=255)
  tCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCaaaaaaa                                                                                                                                                  >  1:2043246/1‑150 (MQ=255)
     aTCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGt                                                                                                                                               >  2:1603915/1‑150 (MQ=255)
         ttCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTtgat                                                                                                                                           <  2:892064/150‑1 (MQ=255)
           cTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTtgatga                                                                                                                                         >  1:1124239/1‑150 (MQ=255)
           cTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTtgatga                                                                                                                                         >  1:4077522/1‑150 (MQ=255)
               cAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGa                                                                                                                                     <  2:4481917/150‑1 (MQ=255)
                aGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGaa                                                                                                                                    >  1:1736746/1‑150 (MQ=255)
                aGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGaa                                                                                                                                    >  2:3233961/1‑150 (MQ=255)
                 gATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAac                                                                                                                                   >  1:1483719/1‑150 (MQ=255)
                 gATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAac                                                                                                                                   <  2:44159/150‑1 (MQ=255)
                 gATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAac                                                                                                                                   >  2:3312857/1‑150 (MQ=255)
                  aTTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTACAGAAAGCAAAAAAACGTTGATGAGTGAaca                                                                                                                                  >  2:4227788/1‑150 (MQ=255)
                    tAAGCCAAACTACCCGTTCGGCAATGCTTCAGTTAAGCGTAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGTTTATGTGTTTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACAcc                                                                                                                                <  1:1297754/150‑1 (MQ=255)
                    tAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACAcc                                                                                                                                >  2:3373107/1‑150 (MQ=255)
                          aaaaTACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACa                                                                                                                          >  2:243063/1‑150 (MQ=255)
                            aaTACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAat                                                                                                                        >  1:74076/1‑150 (MQ=255)
                              tACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAatat                                                                                                                      <  2:1007591/150‑1 (MQ=255)
                                cccGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTc                                                                                                                    >  1:2385542/1‑150 (MQ=255)
                                   gTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAgg                                                                                                                 <  2:3803465/150‑1 (MQ=255)
                                    ttCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGt                                                                                                                >  2:747623/1‑150 (MQ=255)
                                    ttCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGt                                                                                                                <  1:4031213/150‑1 (MQ=255)
                                      cGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAg                                                                                                              <  1:1048733/150‑1 (MQ=255)
                                       ggCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGc                                                                                                             >  1:3915500/1‑150 (MQ=255)
                                          aaTGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAAt                                                                                                          >  2:2782978/1‑150 (MQ=255)
                                            tGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTg                                                                                                        <  1:2234912/150‑1 (MQ=255)
                                                tCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGc                                                                                                    >  1:2848078/1‑150 (MQ=255)
                                                 cAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCt                                                                                                   <  2:1487708/150‑1 (MQ=255)
                                                  aGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCtt                                                                                                  >  1:1779998/1‑150 (MQ=255)
                                                  aGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTCGCAATTGATGCtg                                                                                                  >  1:2898085/1‑149 (MQ=255)
                                                  aGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCtt                                                                                                  <  1:606608/150‑1 (MQ=255)
                                                  aGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCtt                                                                                                  >  1:494817/1‑150 (MQ=255)
                                                  aGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCtt                                                                                                  >  2:2269019/1‑150 (MQ=255)
                                                   gTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCttt                                                                                                 <  2:2118232/150‑1 (MQ=255)
                                                   gTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCttt                                                                                                 <  1:1320104/150‑1 (MQ=255)
                                                     tACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTATCTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTtat                                                                                               <  2:1147318/150‑1 (MQ=255)
                                                     tACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTtat                                                                                               >  1:3752341/1‑150 (MQ=255)
                                                      aCGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTtata                                                                                              >  1:3369360/1‑150 (MQ=255)
                                                         cgAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAg                                                                                           >  2:3162359/1‑150 (MQ=255)
                                                          gAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGc                                                                                          >  1:2795643/1‑150 (MQ=255)
                                                              agGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAAc                                                                                      <  1:3540306/150‑1 (MQ=255)
                                                                gAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGt                                                                                    >  2:2008066/1‑150 (MQ=255)
                                                                 aaTGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTc                                                                                   <  1:2497242/150‑1 (MQ=255)
                                                                 aaTGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTc                                                                                   <  2:1069945/150‑1 (MQ=255)
                                                                   tGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCgg                                                                                 <  1:2838906/150‑1 (MQ=255)
                                                                    ggTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGc                                                                                >  1:522179/1‑150 (MQ=255)
                                                                       cTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTa                                                                             >  1:3745469/1‑150 (MQ=255)
                                                                        tGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTaa                                                                            >  2:3224748/1‑150 (MQ=255)
                                                                         gAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTaaa                                                                           >  1:2331128/1‑150 (MQ=255)
                                                                           aTTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATc                                                                         >  2:3568740/1‑150 (MQ=255)
                                                                            ttCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCg                                                                        >  2:2941948/1‑150 (MQ=255)
                                                                            ttCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCg                                                                        >  2:3132126/1‑150 (MQ=255)
                                                                               tttttttGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCg                                                                     <  1:1001462/150‑1 (MQ=255)
                                                                                 tttttGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCggg                                                                   <  2:3872947/150‑1 (MQ=255)
                                                                                  ttttGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGt                                                                  <  2:1044981/150‑1 (MQ=255)
                                                                                    ttGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGtga                                                                >  1:2922175/1‑150 (MQ=255)
                                                                                       gTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGtgatga                                                             >  2:2625629/1‑150 (MQ=255)
                                                                                          gCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAg                                                          <  2:947700/150‑1 (MQ=255)
                                                                                           cTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGt                                                         <  1:4397808/150‑1 (MQ=255)
                                                                                                 tctcTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAaca                                                   <  2:2850579/150‑1 (MQ=255)
                                                                                                     tGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTa                                               <  2:1999389/150‑1 (MQ=255)
                                                                                                       gAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTAcc                                             <  2:2445631/150‑1 (MQ=255)
                                                                                                         gtgtCTGGATTATGTGATTATTAATGTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCg                                           >  2:2173245/1‑150 (MQ=255)
                                                                                                                   tATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGAc                                 >  1:3422172/1‑150 (MQ=255)
                                                                                                                    aTGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACg                                >  2:2578123/1‑150 (MQ=255)
                                                                                                                     tgtgATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGc                               >  2:463680/1‑150 (MQ=255)
                                                                                                                     tgtgATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGc                               >  2:1823893/1‑150 (MQ=255)
                                                                                                                     tgtgATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGc                               >  2:4279113/1‑150 (MQ=255)
                                                                                                                        gATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTAt                            >  2:3977289/1‑150 (MQ=255)
                                                                                                                        gATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTAt                            >  2:3977210/1‑150 (MQ=255)
                                                                                                                          ttattaATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCt                          <  2:1936176/150‑1 (MQ=255)
                                                                                                                            attaATTTAACTCCAGAAAGCAAAAAAACGATGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTc                        <  1:3234971/150‑1 (MQ=255)
                                                                                                                             ttaATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCg                       >  2:2707240/1‑150 (MQ=255)
                                                                                                                              taATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCgg                      >  1:4190150/1‑150 (MQ=255)
                                                                                                                                aTTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCa                    <  1:3066093/150‑1 (MQ=255)
                                                                                                                                aTTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCa                    >  2:1397139/1‑150 (MQ=255)
                                                                                                                                  ttAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAAc                  >  2:1527389/1‑150 (MQ=255)
                                                                                                                                    aaCTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCa                <  1:2048119/150‑1 (MQ=255)
                                                                                                                                      cTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACg              >  1:3994448/1‑150 (MQ=255)
                                                                                                                                       tCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGt             >  2:586295/1‑150 (MQ=255)
                                                                                                                                       tCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGt             >  1:996557/1‑150 (MQ=255)
                                                                                                                                         cAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTAt           <  1:256223/150‑1 (MQ=255)
                                                                                                                                         cAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTAt           >  2:4213988/1‑150 (MQ=255)
                                                                                                                                          aGAAAGCAAAAAAACGTTGATGAGTGAACTCCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATg          <  2:3685908/150‑1 (MQ=255)
                                                                                                                                          aGAAAGCAAAAAAACGTTGATGAGTGAAAACCGTAACAATATTCAGGTAGCAATTTATGATTTATATAGACAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGAATg          >  1:67020/1‑150 (MQ=255)
                                                                                                                                           gAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATgg         <  2:4634376/150‑1 (MQ=255)
                                                                                                                                             aaGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCa       >  2:2820699/1‑150 (MQ=255)
                                                                                                                                             aaGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCa       <  1:3802039/150‑1 (MQ=255)
                                                                                                                                             aaGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCa       <  2:3553825/150‑1 (MQ=255)
                                                                                                                                             aaGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCa       <  2:4291374/150‑1 (MQ=255)
                                                                                                                                              aGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCaa      >  2:2989022/1‑150 (MQ=255)
                                                                                                                                                 aaaaaaaCGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATATCCAACGTCGGCGTAAATCGCCGGGTGATGAAATTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACa   >  2:2262065/1‑150 (MQ=255)
                                                                                                                                                 aaaaaaaCGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACa   >  2:3591417/1‑150 (MQ=255)
                                                                                                                                                 aaaaaaaCGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACa   >  1:2147611/1‑150 (MQ=255)
                                                                                                                                                 aaaaaaaCGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACa   <  1:877136/150‑1 (MQ=255)
                                                                                                                                                  aaaaaaCGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACAt  >  1:2699956/1‑150 (MQ=255)
                                                                                                                                                  aaaaaaCGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACAt  <  2:3443905/150‑1 (MQ=255)
                                                                                                                                                  aaaaaaCGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACAt  >  1:1598113/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACAT  >  CP009273/1240717‑1241012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑