Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,299,742 C→A 24.5% A244A (GCC→GCA)  oppD → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,299,7420CA24.5% 576.4 / 144.3 232A244A (GCC→GCA) oppDoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (77/98);  new base A (28/29);  total (105/127)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 236 total aligned reads displayed.

CCAACAACTGCGCTGGACGTCACCGTACAGGCGCAGATCATGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTAATCCGCCAAACCTGCTGCG  >  CP009273/1299593‑1299891
                                                                                                                                                     |                                                                                                                                                     
ccAACAACTGCGCTGGACGTCACCGTACAGGCGCAGATCATGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGcc                                                                                                                                                       <  1:2195671/150‑1 (MQ=255)
  aacaacTGCGCTGGACGTCACCGTACAGGCGCAGATCATGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCgg                                                                                                                                                     <  1:2615038/150‑1 (MQ=255)
   acaacTGCGCTGGACGTCACCGTACAGGCGCAGATCATGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCggg                                                                                                                                                    >  2:1518419/1‑150 (MQ=255)
    caacTGCGCTGGACGTCACCGTACAGGCGCAGATCATGACGCTATTAAATGAACTGACGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGgc                                                                                                                                                   <  1:3880978/150‑1 (MQ=255)
    caacTGCGCTGGACGTCACCGTACAGGCGCAGATCATGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGgc                                                                                                                                                   <  1:595094/150‑1 (MQ=255)
    caacTGCGCTGGACGTCACCGTACAGGCGCAGATCATGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGgc                                                                                                                                                   >  1:164959/1‑150 (MQ=255)
     aacTGCGCTGGACGTCACCGTACAGGCGCAGATCATGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCAGCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGgcg                                                                                                                                                  >  2:3730760/1‑150 (MQ=255)
           gcTGGACGTCACCGTACAGGCGCAGATCATGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGAt                                                                                                                                            <  2:347456/150‑1 (MQ=255)
            cTGGACGTCACCGTACAGGCGCAGATCATGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATg                                                                                                                                           <  1:1299853/150‑1 (MQ=255)
                    cACCGTACAGGCGCAGATCATGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATgg                                                                                                                                   >  2:4420590/1‑150 (MQ=255)
                       cGTACAGGCGCAGATCATGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCaa                                                                                                                                <  1:3283712/150‑1 (MQ=255)
                               cgcAGATCATGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAAcgcgcgcg                                                                                                                        >  1:341253/1‑150 (MQ=255)
                                 cAGATCATGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGAt                                                                                                                      >  1:4205663/1‑150 (MQ=255)
                                       aTGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCttt                                                                                                                <  1:2422415/150‑1 (MQ=255)
                                        tGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCtttt                                                                                                               <  2:1170745/150‑1 (MQ=255)
                                          aCGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTAt                                                                                                             <  1:2793114/150‑1 (MQ=255)
                                             cTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCaa                                                                                                          >  2:3769119/1‑150 (MQ=255)
                                               aTTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACACAGTGCTGGTAATTTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAAcc                                                                                                        >  1:906653/1‑150 (MQ=255)
                                                 tacaTGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAAccct                                                                                                      <  2:2724063/147‑2 (MQ=255)
                                                  aaaTGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGt                                                                                                     >  1:3831934/1‑150 (MQ=255)
                                                    aTGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTc                                                                                                   >  1:3948234/1‑150 (MQ=255)
                                                    aTGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTc                                                                                                   <  2:1650492/150‑1 (MQ=255)
                                                       aaCTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATc                                                                                                >  1:2588157/1‑150 (MQ=255)
                                                        aCTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATcc                                                                                               <  1:4629462/150‑1 (MQ=255)
                                                        aCTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATcc                                                                                               <  1:1683958/150‑1 (MQ=255)
                                                         cTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCt                                                                                              <  1:4199551/150‑1 (MQ=255)
                                                          tGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAAGATGGCACCGCGCGCGATGTCTTTTATCAACCCGTTCATCCtt                                                                                             >  2:1034389/1‑150 (MQ=255)
                                                           gAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTa                                                                                            >  2:3267877/1‑150 (MQ=255)
                                                            aaGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTAt                                                                                           <  2:1952060/150‑1 (MQ=255)
                                                             aGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTAtt                                                                                          >  2:2032116/1‑150 (MQ=255)
                                                             aGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTAtt                                                                                          <  2:2191745/150‑1 (MQ=255)
                                                               cGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCt                                                                                        <  1:1712560/150‑1 (MQ=255)
                                                                gggAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTa                                                                                       >  1:1139454/1‑150 (MQ=255)
                                                                gggAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTa                                                                                       >  1:133552/1‑150 (MQ=255)
                                                                gggAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTa                                                                                       >  1:3854411/1‑150 (MQ=255)
                                                                gggAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTa                                                                                       <  1:1869766/150‑1 (MQ=255)
                                                                gggAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTa                                                                                       <  1:3003450/150‑1 (MQ=255)
                                                                   aaTTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCg                                                                                    <  2:358116/150‑1 (MQ=255)
                                                                   aaTTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCg                                                                                    <  1:4411694/150‑1 (MQ=255)
                                                                   aaTTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCg                                                                                    <  1:2636103/150‑1 (MQ=255)
                                                                      ttAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGtt                                                                                 <  2:854480/150‑1 (MQ=255)
                                                                       ttaTACCGACATAATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACTAAGTGCTGGTAATGTACTCAGGGCGCACGATTGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGttt                                                                                <  2:890408/148‑1 (MQ=255)
                                                                       tAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGttt                                                                                <  1:1577359/150‑1 (MQ=255)
                                                                       tAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGttt                                                                                <  2:1871971/150‑1 (MQ=255)
                                                                       tAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGttt                                                                                <  2:1246365/150‑1 (MQ=255)
                                                                        aaTACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTg                                                                               >  2:3904241/1‑150 (MQ=255)
                                                                        aaTACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTg                                                                               <  2:19301/150‑1 (MQ=255)
                                                                           aCCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTc                                                                            >  1:1062599/1‑150 (MQ=255)
                                                                            ccGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCa                                                                           >  2:1633858/1‑150 (MQ=255)
                                                                            ccGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCa                                                                           >  1:1530353/1‑150 (MQ=255)
                                                                              gCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAAc                                                                         >  2:4567224/1‑150 (MQ=255)
                                                                               ccATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAAcg                                                                        <  1:3025290/150‑1 (MQ=255)
                                                                               ccATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAAcg                                                                        <  1:3786601/150‑1 (MQ=255)
                                                                                 atcatTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAAcgcg                                                                      <  1:752600/150‑1 (MQ=255)
                                                                                 atcatTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAAcgcg                                                                      <  1:1806056/150‑1 (MQ=255)
                                                                                   catTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGt                                                                    <  1:1000530/150‑1 (MQ=255)
                                                                                   catTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGt                                                                    <  1:3938075/150‑1 (MQ=255)
                                                                                   catTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGt                                                                    >  1:632507/1‑150 (MQ=255)
                                                                                       aTGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCcg                                                                >  2:2494444/1‑150 (MQ=255)
                                                                                        tGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCcgc                                                               <  1:817010/150‑1 (MQ=255)
                                                                                         gATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCcgcg                                                              >  1:2047682/1‑150 (MQ=255)
                                                                                           tCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGtc                                                            <  1:2583593/150‑1 (MQ=255)
                                                                                           tCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGtc                                                            <  2:3844076/150‑1 (MQ=255)
                                                                                           tCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGtc                                                            >  2:1586729/1‑150 (MQ=255)
                                                                                            cACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGtct                                                           >  1:3857799/1‑150 (MQ=255)
                                                                                              cccACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCg                                                         <  2:3209259/150‑1 (MQ=255)
                                                                                                 aCGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATg                                                      <  1:483724/150‑1 (MQ=255)
                                                                                                 aCGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATg                                                      >  1:2045655/1‑150 (MQ=255)
                                                                                                  cGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGc                                                     <  1:1300497/150‑1 (MQ=255)
                                                                                                   gATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCg                                                    >  2:4166856/1‑150 (MQ=255)
                                                                                                   gATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCg                                                    >  1:445312/1‑150 (MQ=255)
                                                                                                   gATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCg                                                    >  2:194736/1‑150 (MQ=255)
                                                                                                    aTCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCgg                                                   <  1:3495958/150‑1 (MQ=255)
                                                                                                    aTCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCgg                                                   >  1:676071/1‑150 (MQ=255)
                                                                                                    aTCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCgg                                                   <  2:677069/150‑1 (MQ=255)
                                                                                                    aTCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCgg                                                   <  2:2991555/150‑1 (MQ=255)
                                                                                                     tCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGa                                                  >  2:529351/1‑150 (MQ=255)
                                                                                                      cTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGaa                                                 <  1:2987742/150‑1 (MQ=255)
                                                                                                       ttGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAg                                                <  2:3289363/150‑1 (MQ=255)
                                                                                                            gtggtgGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCCTCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGaa                                           >  2:169819/1‑150 (MQ=255)
                                                                                                              ggtggCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAAc                                         >  1:2917023/1‑150 (MQ=255)
                                                                                                                 ggCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAAt                                      >  2:2294702/1‑150 (MQ=255)
                                                                                                                    ggggATCTGCGACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGtt                                   <  1:382054/150‑1 (MQ=255)
                                                                                                                        aTCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGAcc                               <  1:4561004/150‑1 (MQ=255)
                                                                                                                           tGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATc                            <  1:1767555/150‑1 (MQ=255)
                                                                                                                              gACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCg                         <  1:2875388/150‑1 (MQ=255)
                                                                                                                              gACAAAGTGCTGGTAATGTACGCAGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCg                         <  2:2600059/150‑1 (MQ=255)
                                                                                                                                   aGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTaa                    <  2:3445894/150‑1 (MQ=255)
                                                                                                                                   aGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTaa                    <  1:1936552/150‑1 (MQ=255)
                                                                                                                                     tGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTAATc                  <  1:2327463/150‑1 (MQ=255)
                                                                                                                                        tGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTAATCCGc               >  1:53037/1‑150 (MQ=255)
                                                                                                                                         ggTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTAATCCGcc              <  1:3594753/150‑1 (MQ=255)
                                                                                                                                               gTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTAATCCGCCAAACct        <  1:1795296/150‑1 (MQ=255)
                                                                                                                                               gTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTAATCCGCCAAACct        >  1:1541682/1‑150 (MQ=255)
                                                                                                                                               gTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTAATCCGCCAAACct        <  1:1905367/150‑1 (MQ=255)
                                                                                                                                               gTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTAATCCGCCAAACct        >  2:3134204/1‑150 (MQ=255)
                                                                                                                                                tACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTAATCCGCCAAACctg       >  1:4177194/1‑150 (MQ=255)
                                                                                                                                                  cGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTAATCCGCCAAACctgct     >  2:181778/1‑150 (MQ=255)
                                                                                                                                                    ccGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTAATCCGCCAAACCtgctgc   <  1:94258/150‑1 (MQ=255)
                                                                                                                                                     cGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTAATCCGCCAAACCTGCTGCg  >  2:3554787/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAACAACTGCGCTGGACGTCACCGTACAGGCGCAGATCATGACGCTATTAAATGAACTGAAGCGGGAATTTAATACCGCCATCATTATGATCACCCACGATCTTGTCGTGGTGGCGGGGATCTGCGACAAAGTGCTGGTAATGTACGCCGGGCGCACGATGGAATATGGCAACGCGCGCGATGTCTTTTATCAACCCGTTCATCCTTATTCTATCGGTTTGCTCAACGCGGTGCCGCGTCTCGATGCGGAAGGTGAAACAATGTTGACCATCCCGGGTAATCCGCCAAACCTGCTGCG  >  CP009273/1299593‑1299891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑