Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,322,140 T→A 100% T159S (ACG→TCG)  btuR ← cob(I)yrinic acid a,c‑diamide adenosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,322,1400TA97.5% 941.4 / 9.4 277T159S (ACG→TCG) btuRcob(I)yrinic acid a,c‑diamide adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (2/5);  new base A (145/125);  total (147/130)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 277 total aligned reads displayed.

GCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTT  >  CP009273/1321994‑1322286
                                                                                                                                                  |                                                                                                                                                  
gCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGAt                                                                                                                                                 <  2:4329638/150‑1 (MQ=255)
   ggACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAAt                                                                                                                                              <  2:658127/150‑1 (MQ=255)
   ggACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAAt                                                                                                                                              <  2:4189331/150‑1 (MQ=255)
   ggACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAAt                                                                                                                                              <  2:3725873/150‑1 (MQ=255)
   ggACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTCACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAAt                                                                                                                                              >  2:4228887/1‑150 (MQ=255)
    gACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATc                                                                                                                                             <  1:663690/150‑1 (MQ=255)
    gACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATc                                                                                                                                             >  2:1841300/1‑150 (MQ=255)
       gTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCAcc                                                                                                                                          <  2:75388/150‑1 (MQ=255)
       gTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCAcc                                                                                                                                          >  1:2511392/1‑150 (MQ=255)
        tCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCg                                                                                                                                         >  1:3861980/1‑150 (MQ=255)
         cATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGt                                                                                                                                        >  1:3990345/1‑150 (MQ=255)
                 gCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGAt                                                                                                                                <  2:264049/150‑1 (MQ=255)
                       ttACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGAc                                                                                                                          <  2:3409981/150‑1 (MQ=255)
                           aGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTc                                                                                                                      >  2:2174461/1‑150 (MQ=255)
                           aGAATAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCAAGTTTGACGGGGCGTAATTCACTTACCGTATCTGCAAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTc                                                                                                                      <  2:2343307/150‑1 (MQ=255)
                             aTTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCAt                                                                                                                    <  1:1499669/150‑1 (MQ=255)
                              ttAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCAtt                                                                                                                   <  1:1224177/150‑1 (MQ=255)
                              ttAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCAtt                                                                                                                   <  2:1952967/150‑1 (MQ=255)
                                aataatCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTa                                                                                                                 >  1:311662/1‑150 (MQ=255)
                                aataatCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTa                                                                                                                 <  1:210280/150‑1 (MQ=255)
                                   aatCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACg                                                                                                              >  2:3595816/1‑150 (MQ=255)
                                   aatCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACg                                                                                                              >  2:3291988/1‑150 (MQ=255)
                                   aatCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACg                                                                                                              >  2:1137819/1‑150 (MQ=255)
                                       gATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTg                                                                                                          >  1:549731/1‑150 (MQ=255)
                                       gATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTg                                                                                                          >  1:2573261/1‑150 (MQ=255)
                                        aTCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGc                                                                                                         >  1:725834/1‑150 (MQ=255)
                                          cccTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcac                                                                                                       <  2:1033692/150‑1 (MQ=255)
                                          cccTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcac                                                                                                       <  1:3095741/150‑1 (MQ=255)
                                            cTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcacca                                                                                                     <  1:4226623/150‑1 (MQ=255)
                                             tATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcaccac                                                                                                    >  1:893932/1‑150 (MQ=255)
                                              aTCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCAct                                                                                                   >  2:3964410/1‑150 (MQ=255)
                                                cTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActtc                                                                                                 >  2:1336146/1‑150 (MQ=255)
                                                    gcTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGCCGTTCATTTAACGCCTGCACCACTTCTTcc                                                                                             <  1:3367605/150‑1 (MQ=255)
                                                    gcTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTcc                                                                                             >  2:4098017/1‑150 (MQ=255)
                                                    gcTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTcc                                                                                             <  1:1047772/150‑1 (MQ=255)
                                                    gcTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTcc                                                                                             <  1:1047646/150‑1 (MQ=255)
                                                    gcTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTcc                                                                                             >  1:2999944/1‑150 (MQ=255)
                                                       tttACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGt                                                                                          <  1:2454984/150‑1 (MQ=255)
                                                         tACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTgg                                                                                        >  1:1304986/1‑150 (MQ=255)
                                                         tACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTgg                                                                                        >  2:3530122/1‑150 (MQ=255)
                                                         tACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTgg                                                                                        >  1:4525658/1‑150 (MQ=255)
                                                         tACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTgg                                                                                        <  1:3478646/150‑1 (MQ=255)
                                                          acacCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGc                                                                                       >  1:3485438/1‑150 (MQ=255)
                                                              cGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAAt                                                                                   <  2:3836132/150‑1 (MQ=255)
                                                                gCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAg                                                                                 <  2:552288/150‑1 (MQ=255)
                                                                gCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAg                                                                                 <  1:461178/150‑1 (MQ=255)
                                                                 cATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAAAATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGt                                                                                >  1:891035/1‑150 (MQ=255)
                                                                   tCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCa                                                                              <  1:4181447/150‑1 (MQ=255)
                                                                    cAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCAt                                                                             >  2:2820275/1‑150 (MQ=255)
                                                                     aaaCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATa                                                                            >  2:4519265/1‑150 (MQ=255)
                                                                     aaaCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATa                                                                            <  2:1218497/150‑1 (MQ=255)
                                                                       aCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACg                                                                          >  2:4371975/1‑150 (MQ=255)
                                                                       aCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACg                                                                          <  1:1799722/150‑1 (MQ=255)
                                                                       aCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACg                                                                          >  2:1664990/1‑150 (MQ=255)
                                                                         gCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGcc                                                                        <  1:2633853/150‑1 (MQ=255)
                                                                                tGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCatat                                                                 <  1:3646373/150‑1 (MQ=255)
                                                                                tGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCatat                                                                 >  1:2603754/1‑150 (MQ=255)
                                                                                  aCGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTCGATGTGGACGTTCTTTTAACGCCTGCACCACTTCTTCCAGTGGCACATAGTCATACGCCACCATATAc                                                               >  2:3048754/1‑150 (MQ=255)
                                                                                   cGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACg                                                              >  2:2810169/1‑150 (MQ=255)
                                                                                    ggggCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGt                                                             >  2:3970091/1‑150 (MQ=255)
                                                                                     gggCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTc                                                            <  1:2550170/150‑1 (MQ=255)
                                                                                     gggCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTc                                                            >  2:128282/1‑150 (MQ=255)
                                                                                          tAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtc                                                       <  2:2012729/150‑1 (MQ=255)
                                                                                          tAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtc                                                       >  1:3883894/1‑150 (MQ=255)
                                                                                           aaTTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtca                                                      >  1:1748901/1‑150 (MQ=255)
                                                                                             ttCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtcatc                                                    <  1:4047972/150‑1 (MQ=255)
                                                                                              tCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtcatca                                                   >  1:2728073/1‑150 (MQ=255)
                                                                                                  ttACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCa                                               >  2:3454757/1‑150 (MQ=255)
                                                                                                   tACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCaa                                              >  2:1501970/1‑150 (MQ=255)
                                                                                                    aCCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCaaa                                             >  2:1385116/1‑150 (MQ=255)
                                                                                                       gTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAcc                                          >  2:2060020/1‑150 (MQ=255)
                                                                                                            tGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATc                                     <  1:3300432/150‑1 (MQ=255)
                                                                                                             gCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTTCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATcc                                    >  1:3995894/1‑150 (MQ=255)
                                                                                                             gCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATcc                                    <  1:2319361/150‑1 (MQ=255)
                                                                                                              ccAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCa                                   >  1:2679206/1‑150 (MQ=255)
                                                                                                              ccAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCa                                   >  1:4271469/1‑150 (MQ=255)
                                                                                                               cAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAg                                  >  1:1954842/1‑150 (MQ=255)
                                                                                                                 gTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTg                                >  2:4602724/1‑150 (MQ=255)
                                                                                                                    cAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAgg                             >  1:4117529/1‑150 (MQ=255)
                                                                                                                     aataaTATCCCGATGACAACCTCGACTCGAGATAATGACGGTCTGTTGATGTGGACGTTCTTTTAACGCCTGCACCTCTTCTTCCAGTGTCAATTAGTCATACGCCACCATATACGTCAGTTCTTCAAGCATAACCATATACAGTGAGGa                            <  2:2938653/147‑1 (MQ=255)
                                                                                                                     aaGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGa                            >  1:2663865/1‑150 (MQ=255)
                                                                                                                         atatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCATAACCCTATCCAGTGAGGAATCa                        <  2:1083354/150‑1 (MQ=255)
                                                                                                                         atatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCa                        <  1:2327835/150‑1 (MQ=255)
                                                                                                                          tatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCag                       >  2:3008006/1‑150 (MQ=255)
                                                                                                                              ccGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaag                   <  1:2795207/150‑1 (MQ=255)
                                                                                                                              ccGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaag                   <  2:2407683/150‑1 (MQ=255)
                                                                                                                               cGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaagc                  >  2:2479794/1‑150 (MQ=255)
                                                                                                                                gATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaagca                 >  1:3935488/1‑150 (MQ=255)
                                                                                                                                 aTGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCAt                <  1:2312075/150‑1 (MQ=255)
                                                                                                                                  tGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATc               >  1:1849219/1‑150 (MQ=255)
                                                                                                                                  tGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATc               >  1:4465554/1‑150 (MQ=255)
                                                                                                                                   gACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATcc              <  1:4144770/150‑1 (MQ=255)
                                                                                                                                    aCAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCg             >  2:627707/1‑150 (MQ=255)
                                                                                                                                    aCAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCg             >  2:698654/1‑150 (MQ=255)
                                                                                                                                      aaCCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCt           <  2:89590/150‑1 (MQ=255)
                                                                                                                                        ccACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCttt         <  2:1738758/150‑1 (MQ=255)
                                                                                                                                            gACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCAt     >  1:805460/1‑150 (MQ=255)
                                                                                                                                            gACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCAt     >  1:3814956/1‑150 (MQ=255)
                                                                                                                                            gACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGACTGCACCACTTCTTCCATTGGCAAATAGTCATACGACACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCAt     <  1:1354309/150‑1 (MQ=255)
                                                                                                                                             aCCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATg    >  1:2124433/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTT  >  CP009273/1321994‑1322286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑