BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,330,955	G→T	89.3%	D290Y (GAT→TAT)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,330,955	0	G	T	89.3%	680.0 / 82.0	253	D290Y (GAT→TAT)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand): ref base G (9/18); new base T (105/121); total (114/139)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 262 total aligned reads displayed.

CCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTT  >  CP009273/1330808‑1331103

ccGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTAt                                                                                                                                                    >  1:3335728/1‑150 (MQ=255)

   gTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTtatggttatggt                                                                                                                                                 <  1:1000511/150‑1 (MQ=255)

      tCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTg                                                                                                                                              <  2:2386798/150‑1 (MQ=255)

       ccATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTgg                                                                                                                                             <  1:4145363/150‑1 (MQ=255)

         aTGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTGTATGGTTATGGTCTGGAt                                                                                                                                           >  2:671368/1‑150 (MQ=255)

          tGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGAtt                                                                                                                                          <  2:343890/150‑1 (MQ=255)

            cTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCa                                                                                                                                        >  1:1383194/1‑150 (MQ=255)

             ttATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCAc                                                                                                                                       <  1:1361603/150‑1 (MQ=255)

              tATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACt                                                                                                                                      >  1:2136590/1‑150 (MQ=255)

               aTCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTa                                                                                                                                     <  1:1016667/150‑1 (MQ=255)

               aTCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTa                                                                                                                                     >  1:722198/1‑150 (MQ=255)

                  ccGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCg                                                                                                                                  >  1:2947739/1‑150 (MQ=255)

                   cGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGt                                                                                                                                 <  2:4146112/150‑1 (MQ=255)

                    ggATGTAGTGGGCTTCAAACGTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGtt                                                                                                                                <  2:852387/150‑1 (MQ=255)

                        gTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCg                                                                                                                            <  2:3782547/150‑1 (MQ=255)

                         tAGTGGGCTTAAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCgg                                                                                                                           >  2:2541074/1‑150 (MQ=255)

                          aGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGa                                                                                                                          <  2:4399630/150‑1 (MQ=255)

                            tGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATc                                                                                                                        >  1:1343698/1‑150 (MQ=255)

                                cTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCgcgc                                                                                                                    >  2:843370/1‑150 (MQ=255)

                                 ttCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGcc                                                                                                                   >  1:160147/1‑150 (MQ=255)

                                  tCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGcca                                                                                                                  <  2:398775/150‑1 (MQ=255)

                                  tCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGcca                                                                                                                  <  1:639861/150‑1 (MQ=255)

                                   cAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGccac                                                                                                                 >  2:2183052/1‑150 (MQ=255)

                                    aaaCTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGccacc                                                                                                                <  2:902145/150‑1 (MQ=255)

                                    aaaCTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGccacc                                                                                                                <  2:3297236/150‑1 (MQ=255)

                                        ttACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTg                                                                                                            <  1:391296/150‑1 (MQ=255)

                                           ccGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCa                                                                                                         <  2:1982151/150‑1 (MQ=255)

                                            cGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCaa                                                                                                        >  2:3141416/1‑150 (MQ=255)

                                             ggAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAat                                                                                                       <  2:3662625/150‑1 (MQ=255)

                                             ggAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAat                                                                                                       >  2:130865/1‑150 (MQ=255)

                                             ggAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAat                                                                                                       <  1:1783803/150‑1 (MQ=255)

                                             ggAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAat                                                                                                       <  1:1351440/150‑1 (MQ=255)

                                              gAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAata                                                                                                      >  2:2976735/1‑150 (MQ=255)

                                               aaaaTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAatat                                                                                                     <  1:2703608/150‑1 (MQ=255)

                                               aaaaTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAatat                                                                                                     <  2:2783594/150‑1 (MQ=255)

                                                aaaTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTATGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATg                                                                                                    <  1:2674308/150‑1 (MQ=255)

                                                 aaTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGt                                                                                                   >  2:2904052/1‑150 (MQ=255)

                                                   ttACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCg                                                                                                 >  1:2514542/1‑150 (MQ=255)

                                                      cGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCa                                                                                              >  1:4464738/1‑150 (MQ=255)

                                                       gTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAg                                                                                             <  1:2886850/150‑1 (MQ=255)

                                                         gAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGaa                                                                                           >  1:1328878/1‑150 (MQ=255)

                                                          aaGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAat                                                                                          <  2:1354342/150‑1 (MQ=255)

                                                             gTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACCTGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATg                                                                                       <  2:3714785/150‑1 (MQ=255)

                                                               aTTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGTGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGt                                                                                     >  1:1518455/1‑150 (MQ=255)

                                                                ttACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTg                                                                                    >  2:3900724/1‑150 (MQ=255)

                                                                ttACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTg                                                                                    <  2:3942586/150‑1 (MQ=255)

                                                                   ccGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCa                                                                                 <  1:3257756/150‑1 (MQ=255)

                                                                   ccGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCa                                                                                 <  1:1313524/150‑1 (MQ=255)

                                                                   ccGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTACGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCa                                                                                 <  1:4073589/150‑1 (MQ=255)

                                                                    cGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCAc                                                                                <  1:335912/150‑1 (MQ=255)

                                                                     gCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCAcc                                                                               >  1:4271623/1‑150 (MQ=255)

                                                                       cacaGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCtg                                                                             >  2:3613200/1‑150 (MQ=255)

                                                                          aGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTgg                                                                          >  2:2575254/1‑150 (MQ=255)

                                                                          aGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTgg                                                                          >  1:3886755/1‑150 (MQ=255)

                                                                            aCCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGct                                                                        <  2:2344374/150‑1 (MQ=255)

                                                                            aCCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGct                                                                        <  2:4231100/150‑1 (MQ=255)

                                                                             ccTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGctt                                                                       >  2:1011023/1‑150 (MQ=255)

                                                                              cTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttc                                                                      <  1:12487/150‑1 (MQ=255)

                                                                               tGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttct                                                                     <  1:1069992/150‑1 (MQ=255)

                                                                                ggTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttctt                                                                    >  1:2600909/1‑150 (MQ=255)

                                                                                  ttCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTcc                                                                  >  1:2596569/1‑150 (MQ=255)

                                                                                  ttCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTTGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTcc                                                                  <  1:1387047/150‑1 (MQ=255)

                                                                                   tctcACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCATCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTccc                                                                 <  2:2272753/150‑1 (MQ=255)

                                                                                    ctcACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCa                                                                <  1:3774867/150‑1 (MQ=255)

                                                                                    ctcACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCa                                                                <  1:473570/150‑1 (MQ=255)

                                                                                    ctcACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCa                                                                >  2:1255925/1‑150 (MQ=255)

                                                                                     tcACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCaa                                                               <  1:884484/150‑1 (MQ=255)

                                                                                      cACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAAt                                                              >  1:3611577/1‑150 (MQ=255)

                                                                                       aCTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATc                                                             <  1:2155669/150‑1 (MQ=255)

                                                                                       aCTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATc                                                             >  2:489383/1‑150 (MQ=255)

                                                                                            tACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGc                                                        <  1:3160309/150‑1 (MQ=255)

                                                                                             aCCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCt                                                       <  1:1125965/150‑1 (MQ=255)

                                                                                                cAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTa                                                    <  2:3338887/150‑1 (MQ=255)

                                                                                                 aaaTGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTaa                                                   >  1:690214/1‑150 (MQ=255)

                                                                                                  aaTGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAAc                                                  <  1:2541074/150‑1 (MQ=255)

                                                                                                   aTGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAAcc                                                 <  1:2034106/150‑1 (MQ=255)

                                                                                                      ctgcGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTc                                              <  1:39392/150‑1 (MQ=255)

                                                                                                         cgcAAACATGGCGTGGTGGGGAAATTCGTCGAATGTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGAt                                           >  2:4317025/1‑150 (MQ=255)

                                                                                                                  ggCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGt                                  >  1:3534497/1‑150 (MQ=255)

                                                                                                                    cGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGttt                                <  2:2819926/150‑1 (MQ=255)

                                                                                                                         tggGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCg                           <  1:2709923/150‑1 (MQ=255)

                                                                                                                         tggGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCg                           >  1:3640615/1‑150 (MQ=255)

                                                                                                                          ggggAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCgg                          <  2:3462142/150‑1 (MQ=255)

                                                                                                                          ggggAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCgg                          <  1:1914190/150‑1 (MQ=255)

                                                                                                                          ggggAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCgg                          <  1:2360740/150‑1 (MQ=255)

                                                                                                                            ggAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgc                        <  2:2331764/150‑1 (MQ=255)

                                                                                                                               aaTTCGTAGAATTTTTTGGTTATGGTCTGGTTTCTCTACCGTTGGCGGATCGCGCCTCCCTTGCCATTATGTCGCCAGTTTATGGTGCCTCCTGTGGCTTCTTCCCAATCGATGCTGTAAACCTCGTTTACATGCGTTTAAGCGGGCGCa                     <  2:3854800/150‑1 (MQ=255)

                                                                                                                                  tcgtcgAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCg                  >  1:511557/1‑150 (MQ=255)

                                                                                                                                    gtcgAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGaa                >  2:1839938/1‑150 (MQ=255)

                                                                                                                                    gtcgAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCACCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGaa                >  2:211104/1‑150 (MQ=255)

                                                                                                                                     tcgAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAg               <  1:2298310/150‑1 (MQ=255)

                                                                                                                                      cgAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGa              <  2:3078519/150‑1 (MQ=255)

                                                                                                                                       gAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGAt             >  2:179281/1‑150 (MQ=255)

                                                                                                                                       gAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGAt             <  2:3585467/150‑1 (MQ=255)

                                                                                                                                         aTTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCa           <  2:53603/150‑1 (MQ=255)

                                                                                                                                            ttatggttatggTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGt        <  2:170776/150‑1 (MQ=255)

                                                                                                                                               tGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGa     <  2:4534075/150‑1 (MQ=255)

                                                                                                                                               tGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGa     >  1:3475545/1‑150 (MQ=255)

                                                                                                                                               tGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGa     <  2:322016/150‑1 (MQ=255)

                                                                                                                                                ggTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAg    <  1:4080740/150‑1 (MQ=255)

CCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTT  >  CP009273/1330808‑1331103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`