Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 104,561 G→T 100% A124S (GCG→TCG)  secM → regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273104,5610GT95.6% 952.6 / 30.7 293A124S (GCG→TCG) secMregulator of secA translation
Reads supporting (aligned to +/- strand):  ref base G (6/7);  new base T (151/129);  total (157/136)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 296 total aligned reads displayed.

AACTATTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCTAACAA  >  CP009273/104414‑104707
                                                                                                                                                    |                                                                                                                                                  
aaCTATTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAgctcg                                                                                                                                                  >  2:1149597/1‑150 (MQ=255)
    aTTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCgctgc                                                                                                                                              >  2:4222260/1‑150 (MQ=255)
     ttCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCgctgct                                                                                                                                             >  1:4144626/1‑150 (MQ=255)
     ttCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCgctgct                                                                                                                                             >  2:20241/1‑150 (MQ=255)
       ccGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGa                                                                                                                                           <  1:3195941/150‑1 (MQ=255)
       ccGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGa                                                                                                                                           <  1:879197/150‑1 (MQ=255)
        cGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGAc                                                                                                                                          >  2:4460031/1‑150 (MQ=255)
         gTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGAcc                                                                                                                                         <  2:462262/150‑1 (MQ=255)
         gTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGAcc                                                                                                                                         >  2:13106/1‑150 (MQ=255)
           tGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCa                                                                                                                                       >  2:3496478/1‑150 (MQ=255)
           tGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCa                                                                                                                                       >  1:3443262/1‑150 (MQ=255)
             aTTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAgg                                                                                                                                     >  1:2189856/1‑150 (MQ=255)
               tACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGac                                                                                                                                   >  2:3336449/1‑149 (MQ=255)
               tACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGaa                                                                                                                                   >  2:1181461/1‑150 (MQ=255)
               tACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGaa                                                                                                                                   >  2:1536466/1‑150 (MQ=255)
                aCTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAg                                                                                                                                  >  1:308459/1‑150 (MQ=255)
                aCTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAg                                                                                                                                  >  2:1744841/1‑150 (MQ=255)
                aCTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAg                                                                                                                                  >  1:1034313/1‑150 (MQ=255)
                 cTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAgg                                                                                                                                 >  1:285405/1‑150 (MQ=255)
                     cATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACg                                                                                                                             >  1:460383/1‑150 (MQ=255)
                     cATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACg                                                                                                                             >  1:2669940/1‑150 (MQ=255)
                     cATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACg                                                                                                                             >  2:3054439/1‑150 (MQ=255)
                      aTCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGc                                                                                                                            >  1:2809607/1‑150 (MQ=255)
                      aTCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGc                                                                                                                            >  1:3220045/1‑150 (MQ=255)
                       tCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGcc                                                                                                                           >  2:1696086/1‑150 (MQ=255)
                       tCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGcc                                                                                                                           <  2:2313360/150‑1 (MQ=255)
                         aaCATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGt                                                                                                                         <  2:278401/150‑1 (MQ=255)
                           cATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCt                                                                                                                       <  2:1859308/150‑1 (MQ=255)
                           cATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCt                                                                                                                       <  2:2383475/150‑1 (MQ=255)
                            aTGCCATTCGCACGGTAATCCGTCATCTTTCCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCt                                                                                                                       <  2:872550/150‑1 (MQ=255)
                            aTGCCAGTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTg                                                                                                                      <  1:4337943/150‑1 (MQ=255)
                             tGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGa                                                                                                                     <  1:1880866/150‑1 (MQ=255)
                                  ttCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGACAAgg                                                                                                                >  1:1935936/1‑150 (MQ=255)
                                    cGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGt                                                                                                              >  1:1511355/1‑150 (MQ=255)
                                    cGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGt                                                                                                              >  1:4571267/1‑150 (MQ=255)
                                       aCGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCTCGCCGTCTGAAAAGGGTTAt                                                                                                           >  1:3414823/1‑150 (MQ=255)
                                       aCGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTAt                                                                                                           <  2:3715403/150‑1 (MQ=255)
                                         ggTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCg                                                                                                         >  2:1069482/1‑150 (MQ=255)
                                             aTCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAtt                                                                                                     <  1:3067178/150‑1 (MQ=255)
                                              tCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTg                                                                                                    <  1:3589633/150‑1 (MQ=255)
                                                cGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGAt                                                                                                  >  2:46895/1‑150 (MQ=255)
                                                 gTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCTCGCCGTCTGAAAAGGGTTATCGCATTGAtt                                                                                                 >  2:1941140/1‑150 (MQ=255)
                                                 gTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGAtt                                                                                                 <  2:4298247/150‑1 (MQ=255)
                                                    aTCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATg                                                                                              <  1:2098516/150‑1 (MQ=255)
                                                    aTCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATg                                                                                              >  2:1824093/1‑150 (MQ=255)
                                                    aTCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATg                                                                                              <  1:3650967/150‑1 (MQ=255)
                                                       ttt‑ctttcGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATgcgc                                                                                           >  2:2625317/1‑150 (MQ=255)
                                                       ttt‑ctttcGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATgcgc                                                                                           >  2:312903/1‑150 (MQ=255)
                                                        tt‑ctttcGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCa                                                                                          <  1:3132965/150‑1 (MQ=255)
                                                           ctttcGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCAtt                                                                                        <  1:1412400/150‑1 (MQ=255)
                                                             ttcGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCAtttt                                                                                      >  1:1831817/1‑150 (MQ=255)
                                                                 cAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTAccc                                                                                  >  2:4364857/1‑150 (MQ=255)
                                                                    tGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCcac                                                                               <  1:2014048/150‑1 (MQ=255)
                                                                      gCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACaa                                                                             >  2:2896304/1‑150 (MQ=255)
                                                                        aCCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGc                                                                           <  2:3250693/150‑1 (MQ=255)
                                                                             aaaCACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAAt                                                                      <  2:2226533/150‑1 (MQ=255)
                                                                             aaaCACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAAt                                                                      >  1:4312554/1‑150 (MQ=255)
                                                                               acacTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTc                                                                    <  1:178597/150‑1 (MQ=255)
                                                                                cacTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCa                                                                   <  2:4184809/150‑1 (MQ=255)
                                                                                 acTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAg                                                                  <  1:2385219/150‑1 (MQ=255)
                                                                                  cTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGc                                                                 >  1:2585683/1‑150 (MQ=255)
                                                                                    gCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCAc                                                               >  1:360583/1‑150 (MQ=255)
                                                                                     cccGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACg                                                              <  2:4201787/150‑1 (MQ=255)
                                                                                        gTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGccc                                                           <  2:2924111/150‑1 (MQ=255)
                                                                                        gTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGccc                                                           <  2:2580994/150‑1 (MQ=255)
                                                                                           gCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTc                                                        >  1:709581/1‑150 (MQ=255)
                                                                                             tGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTg                                                      <  2:3441660/150‑1 (MQ=255)
                                                                                                 gaaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATa                                                  >  1:3845953/1‑150 (MQ=255)
                                                                                                    tCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGc                                               >  1:101369/1‑150 (MQ=255)
                                                                                                      tttGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCa                                             <  1:2274124/150‑1 (MQ=255)
                                                                                                      tttGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCa                                             >  1:794956/1‑150 (MQ=255)
                                                                                                      tttGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCa                                             >  1:2968493/1‑150 (MQ=255)
                                                                                                      tttGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCa                                             <  1:2329114/150‑1 (MQ=255)
                                                                                                            tctTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCa                                       >  1:2923751/1‑150 (MQ=255)
                                                                                                            tctTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCa                                       >  2:2775/1‑150 (MQ=255)
                                                                                                              ttCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAg                                     >  1:13363/1‑150 (MQ=255)
                                                                                                                cAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGc                                   <  1:4263338/150‑1 (MQ=255)
                                                                                                                 aGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCa                                  >  2:2441431/1‑150 (MQ=255)
                                                                                                                  ggCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCAt                                 >  1:3527392/1‑150 (MQ=255)
                                                                                                                  ggCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCAt                                 >  2:360330/1‑150 (MQ=255)
                                                                                                                    cgcAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATcc                               <  2:3927281/150‑1 (MQ=255)
                                                                                                                    cgcAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATcc                               >  1:1139420/1‑150 (MQ=255)
                                                                                                                     gcAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCg                              >  2:751244/1‑150 (MQ=255)
                                                                                                                          aTCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCCCGCCCGTCTGGATACGCCAGGCGCAAGGCAACCGTGCTg                         <  2:2208934/150‑1 (MQ=255)
                                                                                                                          aTCTTGCATTAATGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAGTGATTATGCGCATTTTACCCCACAAGCAAAATTCATCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTg                         >  1:3724351/1‑150 (MQ=255)
                                                                                                                            cTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGc                       >  2:3061717/1‑150 (MQ=255)
                                                                                                                               gCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCt                    >  1:170942/1‑150 (MQ=255)
                                                                                                                                cATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTc                   >  1:2719964/1‑150 (MQ=255)
                                                                                                                                 aTTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAATGCATCCGTGCTGGCCCTCa                  <  2:3886483/150‑1 (MQ=255)
                                                                                                                                   tACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAAc                <  1:2529890/150‑1 (MQ=255)
                                                                                                                                   tACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAAc                <  1:4082270/150‑1 (MQ=255)
                                                                                                                                      tGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGcc             <  1:4535015/150‑1 (MQ=255)
                                                                                                                                          tACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCAc         <  2:1435500/150‑1 (MQ=255)
                                                                                                                                            cGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCt       <  1:198309/150‑1 (MQ=255)
                                                                                                                                            cGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCt       <  2:1555442/150‑1 (MQ=255)
                                                                                                                                            cGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCt       <  1:2711299/150‑1 (MQ=255)
                                                                                                                                               tCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCTaac    <  1:3208911/150‑1 (MQ=255)
                                                                                                                                                cAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCTaaca   <  1:1108970/150‑1 (MQ=255)
                                                                                                                                                 aGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCTaacaa  >  1:2480244/1‑150 (MQ=255)
                                                                                                                                                 aGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCTaacaa  >  1:3002176/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AACTATTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTT‑CTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCTAACAA  >  CP009273/104414‑104707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑